Stephen P Diggle

Summary

Affiliation: University of Nottingham
Country: UK

Publications

  1. pmc Quorum-sensing and cheating in bacterial biofilms
    Roman Popat
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Proc Biol Sci 279:4765-71. 2012
  2. pmc Characterisation of two quorum sensing systems in the endophytic Serratia plymuthica strain G3: differential control of motility and biofilm formation according to life-style
    Xiaoguang Liu
    Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China
    BMC Microbiol 11:26. 2011
  3. ncbi request reprint The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Environ Microbiol 8:1095-104. 2006
  4. ncbi request reprint Functional genetic analysis reveals a 2-Alkyl-4-quinolone signaling system in the human pathogen Burkholderia pseudomallei and related bacteria
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, UK
    Chem Biol 13:701-10. 2006
  5. ncbi request reprint Cooperation and conflict in quorum-sensing bacterial populations
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Nature 450:411-4. 2007
  6. ncbi request reprint The Pseudomonas aeruginosa 4-quinolone signal molecules HHQ and PQS play multifunctional roles in quorum sensing and iron entrapment
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    Chem Biol 14:87-96. 2007
  7. pmc Evolutionary theory of bacterial quorum sensing: when is a signal not a signal?
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Philos Trans R Soc Lond B Biol Sci 362:1241-9. 2007
  8. ncbi request reprint 4-quinolone signalling in Pseudomonas aeruginosa: old molecules, new perspectives
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Int J Med Microbiol 296:83-91. 2006
  9. doi request reprint Microbial communication and virulence: lessons from evolutionary theory
    Stephen P Diggle
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University Park, University of Nottingham, NG7 2RD, UK
    Microbiology 156:3503-12. 2010
  10. ncbi request reprint Quorum sensing
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Curr Biol 17:R907-10. 2007

Collaborators

Detail Information

Publications29

  1. pmc Quorum-sensing and cheating in bacterial biofilms
    Roman Popat
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Proc Biol Sci 279:4765-71. 2012
    ..Finally, we discuss the clinical implications that arise from altering the susceptibility to antibiotics...
  2. pmc Characterisation of two quorum sensing systems in the endophytic Serratia plymuthica strain G3: differential control of motility and biofilm formation according to life-style
    Xiaoguang Liu
    Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China
    BMC Microbiol 11:26. 2011
    ..An endophytic Serratia sp. G3 with biocontrol potential and high levels of AHL signal production was isolated from the stems of wheat and the role of QS in this isolate was determined...
  3. ncbi request reprint The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Environ Microbiol 8:1095-104. 2006
    ..Similar results were also obtained for biofilms grown under dynamic flow conditions on steel coupons, suggesting that LecA contributes to P. aeruginosa biofilm architecture under different environmental conditions...
  4. ncbi request reprint Functional genetic analysis reveals a 2-Alkyl-4-quinolone signaling system in the human pathogen Burkholderia pseudomallei and related bacteria
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, UK
    Chem Biol 13:701-10. 2006
    ..These data reveal a role for AHQs in bacterial cell-to-cell communication beyond that seen in P. aeruginosa...
  5. ncbi request reprint Cooperation and conflict in quorum-sensing bacterial populations
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Nature 450:411-4. 2007
    ..These results show that the problem of exploitation, which has been the focus of considerable attention in animal communication, also arises in bacteria...
  6. ncbi request reprint The Pseudomonas aeruginosa 4-quinolone signal molecules HHQ and PQS play multifunctional roles in quorum sensing and iron entrapment
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    Chem Biol 14:87-96. 2007
    ....
  7. pmc Evolutionary theory of bacterial quorum sensing: when is a signal not a signal?
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Philos Trans R Soc Lond B Biol Sci 362:1241-9. 2007
    ....
  8. ncbi request reprint 4-quinolone signalling in Pseudomonas aeruginosa: old molecules, new perspectives
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Int J Med Microbiol 296:83-91. 2006
    ..Furthermore, 4Q signalling is not restricted to P. aeruginosa raising the possibility of cross-talk with other related bacterial species which occupy similar ecological niches...
  9. doi request reprint Microbial communication and virulence: lessons from evolutionary theory
    Stephen P Diggle
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University Park, University of Nottingham, NG7 2RD, UK
    Microbiology 156:3503-12. 2010
    ..adaptive approaches to microbial behaviours we can begin to address questions such as, what factors influence cooperation and the evolution of virulence in microbes and can we exploit these factors to develop new antimicrobial strategies?..
  10. ncbi request reprint Quorum sensing
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Curr Biol 17:R907-10. 2007
  11. ncbi request reprint The Pseudomonas aeruginosa quinolone signal molecule overcomes the cell density-dependency of the quorum sensing hierarchy, regulates rhl-dependent genes at the onset of stationary phase and can be produced in the absence of LasR
    Stephen P Diggle
    Institute of Infection, Immunity and Inflammation, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2RD, UK
    Mol Microbiol 50:29-43. 2003
    ..Increased expression of lecA in the presence of exogenous PQS can be explained partially by increases in RhlR, RpoS and C4-HSL levels. A refined model for quorum sensing in P. aeruginosa is presented...
  12. ncbi request reprint A dual biosensor for 2-alkyl-4-quinolone quorum-sensing signal molecules
    Matthew P Fletcher
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, UK
    Environ Microbiol 9:2683-93. 2007
    ..We also noted that on TLC plates the biosensor not only produced bioluminescence in response to AHQs but also the green pigment, pyocyanin which offers an alternative visual indicator for AHQ production...
  13. pmc Quinolones: from antibiotics to autoinducers
    Stephan Heeb
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham, UK
    FEMS Microbiol Rev 35:247-74. 2011
    ....
  14. pmc Advancing the quorum in Pseudomonas aeruginosa: MvaT and the regulation of N-acylhomoserine lactone production and virulence gene expression
    Stephen P Diggle
    School of Pharmaceutical Sciences Institute of Infections and Immunity, University Park, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    J Bacteriol 184:2576-86. 2002
    ..Furthermore, addition of exogenous 3O-C12-HSL and C4-HSL to the mvaT mutant significantly advanced lecA expression, suggesting that MvaT is involved in the growth phase-dependent regulation of the lecA gene...
  15. pmc Bursting the bubble on bacterial biofilms: a flow cell methodology
    Shanika A Crusz
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Biofouling 28:835-42. 2012
    ....
  16. ncbi request reprint Biosensor-based assays for PQS, HHQ and related 2-alkyl-4-quinolone quorum sensing signal molecules
    Matthew P Fletcher
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Nat Protoc 2:1254-62. 2007
    ..However, apart from PQS and HHQ in P. aeruginosa, definitive AHQ identification will require additional MS and NMR analyses...
  17. doi request reprint Detection of 2-alkyl-4-quinolones using biosensors
    Stephen P Diggle
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, UK
    Methods Mol Biol 692:21-30. 2011
    ..The assays described are simple to perform, do not require sophisticated instrumentation, and are highly amenable to screening large numbers of bacterial samples...
  18. ncbi request reprint Cooperation, quorum sensing, and evolution of virulence in Staphylococcus aureus
    Eric J G Pollitt
    School of Life Sciences, University of Nottingham, Nottingham, United Kingdom
    Infect Immun 82:1045-51. 2014
    ....
  19. doi request reprint The social behaviours of bacterial pathogens
    Roman Popat
    Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Br Med Bull 87:63-75. 2008
    ..The term quorum sensing (QS) is used to describe communication between bacterial cells, whereby a coordinated population response is controlled by diffusible signal molecules produced by individuals...
  20. ncbi request reprint Quorum sensing by 2-alkyl-4-quinolones in Pseudomonas aeruginosa and other bacterial species
    Jean Frédéric Dubern
    Institute of Infection, Immunity and Inflammation, Centre of Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom
    Mol Biosyst 4:882-8. 2008
    ..Here, we summarize the most recent findings on the biosynthesis and regulation of PQS and other AQs including the discovery of AQs in other bacterial species...
  21. pmc Density-dependent fitness benefits in quorum-sensing bacterial populations
    Sophie E Darch
    School of Molecular Medical Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    Proc Natl Acad Sci U S A 109:8259-63. 2012
    ..Overall, these results support the idea that QS is used to coordinate the switching on of social behaviors at high densities when such behaviors are more efficient and will provide the greatest benefit...
  22. pmc Kin selection, quorum sensing and virulence in pathogenic bacteria
    Kendra P Rumbaugh
    Department of Surgery, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Proc Biol Sci 279:3584-8. 2012
    ....
  23. ncbi request reprint The MexGHI-OpmD multidrug efflux pump controls growth, antibiotic susceptibility and virulence in Pseudomonas aeruginosa via 4-quinolone-dependent cell-to-cell communication
    Séverine Aendekerk
    Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Building E, room 6 6, Pleinlaan 2, B 1050 Brussels, Belgium
    Microbiology 151:1113-25. 2005
    ..Collectively, these results demonstrate an essential function for the MexGHI-OpmD pump in facilitating cell-to-cell communication, antibiotic susceptibility and promoting virulence and growth in P. aeruginosa...
  24. ncbi request reprint Social evolution theory for microorganisms
    Stuart A West
    Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, King s Buildings, Edinburgh, EH9 3JT, UK
    Nat Rev Microbiol 4:597-607. 2006
    ....
  25. doi request reprint Quorum quenching activity in Anabaena sp. PCC 7120: identification of AiiC, a novel AHL-acylase
    Manuel Romero
    Departamento de Microbiologia y Parasitologia, Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago, Spain
    FEMS Microbiol Lett 280:73-80. 2008
    ....
  26. pmc Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1
    Charles F Sio
    Pharmaceutical Biology, University Centre for Pharmacy, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
    Infect Immun 74:1673-82. 2006
    ..The quorum-quenching AHL acylase may enable P. aeruginosa PAO1 to modulate its own quorum-sensing-dependent pathogenic potential and, moreover, offers possibilities for novel antipseudomonal therapies...
  27. ncbi request reprint Quorum sensing regulates dpsA and the oxidative stress response in Burkholderia pseudomallei
    Putthapoom Lumjiaktase
    Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Rama VI Road, Bangkok 10400, Thailand
    Microbiology 152:3651-9. 2006
    ..These data indicate that in B. pseudomallei, QS regulates the response to oxidative stress at least in part via the BpsR/C8-HSL-dependent regulation of DpsA...
  28. pmc Biofilm formation in Pseudomonas aeruginosa: fimbrial cup gene clusters are controlled by the transcriptional regulator MvaT
    Isabelle Vallet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, UPR9027, IBSM CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 186:2880-90. 2004
    ..Thus, MvaT appears to be an important regulatory component within a complex network that controls biofilm formation and maturation in P. aeruginosa...
  29. ncbi request reprint The Pseudomonas aeruginosa global regulator MvaT specifically binds to the ptxS upstream region and enhances ptxS expression
    Landon W Westfall
    Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Microbiology 150:3797-806. 2004
    ..The expression of ptxS was lower in PAODeltamvaT than in PAO1, but no difference was detected in ptxR expression. These results suggest that MvaT positively regulates ptxS expression by binding specifically to the ptxS upstream region...