Christopher Dennison

Summary

Affiliation: University of Newcastle
Country: UK

Publications

  1. pmc Crosstalk between Cu(i) and Zn(ii) homeostasis via Atx1 and cognate domains
    Adriana Badarau
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK
    Chem Commun (Camb) 49:8000-2. 2013
  2. ncbi request reprint The active-site structure of umecyanin, the stellacyanin from horseradish roots
    Christopher Dennison
    School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, UK
    J Am Chem Soc 126:2481-9. 2004
  3. ncbi request reprint Paramagnetic 1H NMR spectrum of the cobalt(II) derivative of spinach plastocyanin
    Christopher Dennison
    School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
    Inorg Chem 43:1502-10. 2004
  4. doi request reprint The role of ligand-containing loops at copper sites in proteins
    Christopher Dennison
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, UK
    Nat Prod Rep 25:15-24. 2008
  5. pmc Alkaline transition of phytocyanins: a comparison of stellacyanin and umecyanin
    Christopher Dennison
    School of Natural Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
    Biochem J 371:377-83. 2003
  6. ncbi request reprint Unusual properties of plastocyanin from the fern Dryopteris crassirhizoma
    Christopher Dennison
    Department of Chemistry, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
    Biochemistry 41:552-60. 2002
  7. doi request reprint Pi-interaction tuning of the active site properties of metalloproteins
    Sachiko Yanagisawa
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK
    J Am Chem Soc 130:15420-8. 2008
  8. doi request reprint FutA2 is a ferric binding protein from Synechocystis PCC 6803
    Adriana Badarau
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Biol Chem 283:12520-7. 2008
  9. doi request reprint The importance of the long type 1 copper-binding loop of nitrite reductase for structure and function
    Katsuko Sato
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, NE2 4HH UK
    Chemistry 14:5820-8. 2008
  10. ncbi request reprint Transient homodimer interactions studied using the electron self-exchange reaction
    Katsuko Sato
    Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Biol Chem 280:19281-8. 2005

Collaborators

Detail Information

Publications43

  1. pmc Crosstalk between Cu(i) and Zn(ii) homeostasis via Atx1 and cognate domains
    Adriana Badarau
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK
    Chem Commun (Camb) 49:8000-2. 2013
    ..Copper and zinc homeostasis could be linked by metal binding to these CXXC-containing proteins. ..
  2. ncbi request reprint The active-site structure of umecyanin, the stellacyanin from horseradish roots
    Christopher Dennison
    School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, UK
    J Am Chem Soc 126:2481-9. 2004
    ....
  3. ncbi request reprint Paramagnetic 1H NMR spectrum of the cobalt(II) derivative of spinach plastocyanin
    Christopher Dennison
    School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
    Inorg Chem 43:1502-10. 2004
    ....
  4. doi request reprint The role of ligand-containing loops at copper sites in proteins
    Christopher Dennison
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, UK
    Nat Prod Rep 25:15-24. 2008
    ..Given the widespread occurrence of -barrel domains in nature, and the array of metal sites in proteins composed of loop regions, the studies described on this model system have implications for a variety of metalloproteins...
  5. pmc Alkaline transition of phytocyanins: a comparison of stellacyanin and umecyanin
    Christopher Dennison
    School of Natural Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
    Biochem J 371:377-83. 2003
    ..This lysine residue is completely conserved in known phytocyanin sequences. Also highlighted in these studies are the remarkable active-site similarities between stellacyanin and umecyanin...
  6. ncbi request reprint Unusual properties of plastocyanin from the fern Dryopteris crassirhizoma
    Christopher Dennison
    Department of Chemistry, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
    Biochemistry 41:552-60. 2002
    ..The self-exchange rate constant increases to 9 x 10(3) M(-1) s(-1) at pH* 5.4, consistent with enhanced protein-protein association at lower pH*, and the absence of His87 protonation in D. crassirhizoma PCu(I) in the accessible pH range...
  7. doi request reprint Pi-interaction tuning of the active site properties of metalloproteins
    Sachiko Yanagisawa
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK
    J Am Chem Soc 130:15420-8. 2008
    ..Protonation of the His ligand is the feature most affected by the introduction of a pi-interaction...
  8. doi request reprint FutA2 is a ferric binding protein from Synechocystis PCC 6803
    Adriana Badarau
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Biol Chem 283:12520-7. 2008
    ..Here, we also discover that there is a reduced level of ferric FutA2 in the periplasm of the DeltafutA1 mutant providing an explanation for its severe iron-uptake phenotype...
  9. doi request reprint The importance of the long type 1 copper-binding loop of nitrite reductase for structure and function
    Katsuko Sato
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, NE2 4HH UK
    Chemistry 14:5820-8. 2008
    ..The extended type 1 copper-binding loop of this enzyme makes a number of interactions that are important for maintaining quaternary structure...
  10. ncbi request reprint Transient homodimer interactions studied using the electron self-exchange reaction
    Katsuko Sato
    Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Biol Chem 280:19281-8. 2005
    ..The physiologically relevant Mg2+ ion has a much more pronounced influence on the k(ese) of spinach plastocyanin, which along with the known properties of the thylakoid lumen suggests a biological role for electron self-exchange...
  11. ncbi request reprint Loop-contraction mutagenesis of type 1 copper sites
    Sachiko Yanagisawa
    School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, UK
    J Am Chem Soc 126:15711-9. 2004
    ..Thus, the ligand-containing loop plays an important role in tuning the entatic nature of a type 1 copper center...
  12. ncbi request reprint Engineering copper sites in proteins: loops confer native structures and properties to chimeric cupredoxins
    Chan Li
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, United Kingdom
    J Am Chem Soc 129:709-18. 2007
    ..This contributes to the observed instability of PCAZ and highlights that loop-scaffold interactions are important for stabilizing the active site of a cupredoxin...
  13. ncbi request reprint The role of hydrogen bonding at the active site of a cupredoxin: the Phe114Pro azurin variant
    Sachiko Yanagisawa
    Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom
    Biochemistry 45:8812-22. 2006
    ..These changes are larger than those seen in the WT protein and contribute to the order of magnitude decrease in the intrinsic electron-transfer capabilities of the Phe114Pro variant...
  14. pmc Variations in methanobactin structure influences copper utilization by methane-oxidizing bacteria
    Abdelnasser El Ghazouani
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    Proc Natl Acad Sci U S A 109:8400-4. 2012
    ..The differences in structures and properties of mbtins, and their influence on copper utilization by methane-oxidizing bacteria, have important implications for the ecology and global function of these environmentally vital organisms...
  15. ncbi request reprint Investigating the cause of the alkaline transition of phytocyanins
    Mark D Harrison
    Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    Biochemistry 44:3056-64. 2005
    ..Other possible triggers for the effects seen in the phytocyanins at elevated pH are discussed along with the relevance of the alkaline transition...
  16. doi request reprint Protein-folding location can regulate manganese-binding versus copper- or zinc-binding
    Steve Tottey
    Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle NE2 4HH, UK
    Nature 455:1138-42. 2008
    ..They explain why the cytoplasm must contain only tightly bound and buffered copper and Zn(2+)...
  17. pmc Basic requirements for a metal-binding site in a protein: the influence of loop shortening on the cupredoxin azurin
    Chan Li
    Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom
    Proc Natl Acad Sci U S A 103:7258-63. 2006
    ....
  18. doi request reprint The influence of protein folding on the copper affinities of trafficking and target sites
    Stephen Allen
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
    Dalton Trans 42:3233-9. 2013
    ....
  19. ncbi request reprint Introduction of a pi-pi interaction at the active site of a cupredoxin: characterization of the Met16Phe Pseudoazurin mutant
    Sachiko Yanagisawa
    School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, United Kingdom
    Biochemistry 42:6853-62. 2003
    ..The enhanced electron transfer reactivity of Met16Phe PACu is a consequence of a lower reorganization energy due to additional active site rigidity caused by the pi-pi interaction between His81 and the introduced phenyl ring...
  20. ncbi request reprint Loop-contraction mutagenesis of a type 1 copper site
    Sachiko Yanagisawa
    School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
    J Am Chem Soc 125:4974-5. 2003
    ..The loop contraction results in the increase of the pKa for the detachable His ligand of pseudoazurin by two pH units, similar to the value as found in amicyanin...
  21. ncbi request reprint Pseudospecificity of the acidic patch of plastocyanin for the interaction with cytochrome f
    Katsuko Sato
    School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, UK
    J Am Chem Soc 126:3028-9. 2004
    ..The only requirement of a plant PCu to ensure efficient interaction with its cyt f is the presence of acidic residues on the periphery of the protein's hydrophobic patch...
  22. ncbi request reprint Reduction potential tuning at a type 1 copper site does not compromise electron transfer reactivity
    Sachiko Yanagisawa
    Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    J Am Chem Soc 127:16453-9. 2005
    ..The enhanced reactivity of a three-coordinate T1 copper site will facilitate intramolecular ET in fungal laccases and Fet3p...
  23. ncbi request reprint Effect of histidine 6 protonation on the active site structure and electron-transfer capabilities of pseudoazurin from Achromobacter cycloclastes
    Katsuko Sato
    Department of Chemistry, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, UK
    Biochemistry 41:120-30. 2002
    ..6 (3.7 x 10(3) M(-1) s(-1)). The effect on the self-exchange reactivity is mainly due to an alteration in the reorganization energy of the copper site brought about by the structural change resulting from His6 protonation...
  24. ncbi request reprint Active-site structure and electron-transfer reactivity of plastocyanins
    Katsuko Sato
    Department of Chemistry, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK
    J Am Chem Soc 125:2101-12. 2003
    ..The effect of the protonation of the His87 ligand in PCu(I) on the ESE reactivity has been investigated. When the influence of the acidic patch is minimized, the ESE rate constant decreases at high [H(+)]...
  25. pmc Metal-binding loop length and not sequence dictates structure
    Katsuko Sato
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    Proc Natl Acad Sci U S A 106:5616-21. 2009
    ..Cupredoxins function as redox shuttles and are required to be monomeric; therefore, none have evolved with a metal-binding loop of this length...
  26. ncbi request reprint Active site comparison of CoII blue and green nitrite reductases
    Katsuko Sato
    Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK
    Chemistry 12:6647-59. 2006
    ..This indicates an alteration in the bonding of the axial ligand in these derivatives, which could be influential in the CuII proteins...
  27. ncbi request reprint Characterization of Arabidopsis thaliana stellacyanin: a comparison with umecyanin
    Mark D Harrison
    School of Natural Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
    Proteins 55:426-35. 2004
    ..7 are obtained for the alkaline transitions in both proteins. This study demonstrates that BCB is the A. thaliana stellacyanin and the results form the biochemical basis for a discussion of BCB function in the model vascular plant...
  28. doi request reprint Cu(I) affinities of the domain 1 and 3 sites in the human metallochaperone for Cu,Zn-superoxide dismutase
    Stephen Allen
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    Biochemistry 51:1439-48. 2012
    ..The Cu(I) affinity of domain 1 of CCS is comparable to that of HAH1, another cytosolic copper metallochaperone. CCS and HAH1 readily exchange Cu(I), providing a mechanism whereby cross-talk can occur between copper trafficking pathways...
  29. pmc Thermodynamics of copper and zinc distribution in the cyanobacterium Synechocystis PCC 6803
    Adriana Badarau
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    Proc Natl Acad Sci U S A 108:13007-12. 2011
    ..The narrow range of Cu(I) affinities for the cytoplasmic copper proteins in Synechocystis will facilitate relocation when copper is limiting...
  30. doi request reprint Visualizing the metal-binding versatility of copper trafficking sites
    Adriana Badarau
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
    Biochemistry 49:7798-810. 2010
    ..A copper-induced switch in Atx1 dimer structure may have a regulatory role with cluster formation helping to buffer copper...
  31. ncbi request reprint An axial met ligand at a type 1 copper site is preferable for fast electron transfer
    Mark D Harrison
    School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, UK
    Chembiochem 5:1579-81. 2004
  32. doi request reprint Copper-binding properties and structures of methanobactins from Methylosinus trichosporium OB3b
    Abdelnasser El Ghazouani
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom
    Inorg Chem 50:1378-91. 2011
    ..5. The two thiolates can also bind Cu(I), albeit with much lower affinity (∼ 3 × 10(15) M(-1) at pH 7.5). The high affinity of mbs for Cu(I) is consistent with a physiological role in copper uptake and protection...
  33. ncbi request reprint Transient protein interactions between cytochrome f and the phytocyanin umecyanin
    Katsuko Sato
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK
    Chembiochem 8:732-5. 2007
  34. doi request reprint Copper trafficking mechanism of CXXC-containing domains: insight from the pH-dependence of their Cu(I) affinities
    Adriana Badarau
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK
    J Am Chem Soc 133:2983-8. 2011
    ..The thermodynamic gradient for Cu(I) transfer between HAH1 and MNK1 depends on pH. The decrease in the pK(a) of the Cys ligand in HAH1 can also influence the kinetics of Cu(I) transfer...
  35. ncbi request reprint A periplasmic iron-binding protein contributes toward inward copper supply
    Kevin J Waldron
    Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle NE4 2HH, United Kingdom
    J Biol Chem 282:3837-46. 2007
    ..It is proposed that FutA2 contributes to metal partitioning in the periplasm by sequestering Fe(III), which limits aberrant Fe(III) associations with vital binding sites for other metals, including copper...
  36. pmc The parsley plastocyanin-turnip cytochrome f complex: a structurally distorted but kinetically functional acidic patch
    Peter B Crowley
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av da Republica, Apartado 127, 2781 901 Oeiras, Portugal
    Biochem J 378:45-51. 2004
    ..The results of NMR studies and protein-docking simulations indicate that parsley PCu and its spinach analogue adopt different orientations in their complexes with cyt f...
  37. ncbi request reprint Influence of loop shortening on the metal binding site of cupredoxin pseudoazurin
    Milko Velarde
    Abteilung Proteomics und Signaltransduktion, Max Planck Institut fur Biochemie, Am Klopferspitz 18, 82152 Martinsried, Germany
    Biochemistry 46:9981-91. 2007
    ..The structure of PAZAMI demonstrates the importance of loop-scaffold interactions for metal sites in proteins...
  38. ncbi request reprint NMR structural analysis of cadmium sensing by winged helix repressor CmtR
    Lucia Banci
    Department of Chemistry and Centro Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, Florence 50019, Italy
    J Biol Chem 282:30181-8. 2007
    ..Cadmium binding across the dimer interface impairs DNA association by excluding the apo-conformers suited to bind DNA...
  39. ncbi request reprint Thermodynamics of the alkaline transition in phytocyanins
    Gianantonio Battistuzzi
    Department of Chemistry, University of Modena and Reggio Emilia, Via Campi 183, 41100 Modena, Italy
    J Biol Inorg Chem 12:895-900. 2007
    ..The data for axial ligand variants are consistent with the hypothesis of a copper-binding His as the deprotonating residue responsible for this transition...
  40. ncbi request reprint Ultrafast pump-probe study of excited-state charge-transfer dynamics in umecyanin from horseradish root
    Ines Delfino
    Biophysics and Nanoscience Centre, CNISM Università della Tuscia, I 01100 Viterbo, Italy
    J Phys Chem B 110:17252-9. 2006
    ..Finally, the Fourier transform of the oscillatory component of the signal presents additional bands in the low-frequency region which are assigned to collective motions of the protein...
  41. ncbi request reprint Optical spectroscopic investigation of the alkaline transition in umecyanin from horseradish root
    Ines Delfino
    Biophysics and Nanoscience Centre, INFM CNISM, Dipartimento Scienze Ambientali, Universita della Tuscia, Largo dell Universita, I 01100 Viterbo, Italy
    Biochemistry 44:16090-7. 2005
    ..A weakening of the Cu-S(Cys) bond is also observed which, along with a weakened axial interaction, must be due to the enhanced Cu-N(His) interaction...
  42. ncbi request reprint Crystal structures of oxidized and reduced stellacyanin from horseradish roots
    Michael Koch
    Abteilung Strukturforschung, Max Planck Institut fur Biochemie, Am Klopferspitz 18, D 82152 Martinsried, Germany
    J Am Chem Soc 127:158-66. 2005
    ....
  43. ncbi request reprint BACE1 cytoplasmic domain interacts with the copper chaperone for superoxide dismutase-1 and binds copper
    Barbara Angeletti
    Neurology and Gastrointestinal Centre of Excellence for Drug Discovery, GlaxoSmithKline, New Frontiers Science Park, Harlow, Essex CM19 5AW, United Kingdom
    J Biol Chem 280:17930-7. 2005
    ..Finally, we demonstrate that the twenty-four residue C-terminal domain of BACE1 binds a single Cu(I) atom with high affinity through cysteine residues...