D W Denning

Summary

Affiliation: University of Manchester
Country: UK

Publications

  1. pmc Mold sensitization is common amongst patients with severe asthma requiring multiple hospital admissions
    B Ronan O'Driscoll
    Department of Respiratory Medicine, Salford Royal Hospitals NHS Trust, Hope Hospital, Salford M6 8HD, UK
    BMC Pulm Med 5:4. 2005
  2. ncbi request reprint Echinocandin antifungal drugs
    David W Denning
    Education and Research Centre, Wythenshawe Hospital, Southmoor Road, M23 9LT, Manchester, UK
    Lancet 362:1142-51. 2003
  3. ncbi request reprint Chronic cavitary and fibrosing pulmonary and pleural aspergillosis: case series, proposed nomenclature change, and review
    David W Denning
    School of Medicine, University of Manchester, Manchester, United Kingdom
    Clin Infect Dis 37:S265-80. 2003
  4. ncbi request reprint British Society for Medical Mycology proposed standards of care for patients with invasive fungal infections
    David W Denning
    School of Medicine, University of Manchester and Wythenshawe Hospital, Manchester, UK
    Lancet Infect Dis 3:230-40. 2003
  5. ncbi request reprint Echinocandins: a new class of antifungal
    David W Denning
    Education and Research Centre, Wythenshawe Hospital, Southmoor Road, Wythenshawe, Manchester M23 9LT, UK
    J Antimicrob Chemother 49:889-91. 2002
  6. ncbi request reprint Sequencing the Aspergillus fumigatus genome
    David W Denning
    School of Medicine, University of Manchester, Manchester, UK
    Lancet Infect Dis 2:251-3. 2002
  7. ncbi request reprint Efficacy and safety of voriconazole in the treatment of acute invasive aspergillosis
    David W Denning
    North Manchester General Hospital, University of Manchester, Manchester, United Kingdom
    Clin Infect Dis 34:563-71. 2002
  8. ncbi request reprint Muco-cutaneous retinoid-effects and facial erythema related to the novel triazole antifungal agent voriconazole
    D W Denning
    University of Manchester School of Medicine, Department of Infectious Diseases and Tropical Medicine, Monsall Unit, North Manchester General Hospital, Manchester, UK
    Clin Exp Dermatol 26:648-53. 2001
  9. doi request reprint Major variations in Aspergillus fumigatus arising within aspergillomas in chronic pulmonary aspergillosis
    S J Howard
    School of Translational Medicine, Manchester Academic Health Science Centre, University Hospital of South Manchester, University of Manchester, UK
    Mycoses 56:434-41. 2013
  10. pmc Susceptibility testing of Aspergillus flavus: inoculum dependence with itraconazole and lack of correlation between susceptibility to amphotericin B in vitro and outcome in vivo
    J Mosquera
    Section of Infectious Diseases, Department of Medicine, Hope Hospital, University of Manchester, United Kingdom
    Antimicrob Agents Chemother 45:1456-62. 2001

Detail Information

Publications94

  1. pmc Mold sensitization is common amongst patients with severe asthma requiring multiple hospital admissions
    B Ronan O'Driscoll
    Department of Respiratory Medicine, Salford Royal Hospitals NHS Trust, Hope Hospital, Salford M6 8HD, UK
    BMC Pulm Med 5:4. 2005
    ..We studied the relationship between asthma severity and immediate type hypersensitivity to mold (fungal) and non-mold allergens in 181 asthmatic subjects...
  2. ncbi request reprint Echinocandin antifungal drugs
    David W Denning
    Education and Research Centre, Wythenshawe Hospital, Southmoor Road, M23 9LT, Manchester, UK
    Lancet 362:1142-51. 2003
    ..Absence of antagonism in combination with other antifungal drugs suggests that combination antifungal therapy could become a general feature of the echinocandins, particularly for invasive aspergillosis...
  3. ncbi request reprint Chronic cavitary and fibrosing pulmonary and pleural aspergillosis: case series, proposed nomenclature change, and review
    David W Denning
    School of Medicine, University of Manchester, Manchester, United Kingdom
    Clin Infect Dis 37:S265-80. 2003
    ..Surgery removed disease but postoperative pleural aspergillosis was inevitable. Indicators of good long-term medical outcomes were mild symptoms, thin-walled quiescent cavities, residual pleural fibrosis, and normal inflammatory markers...
  4. ncbi request reprint British Society for Medical Mycology proposed standards of care for patients with invasive fungal infections
    David W Denning
    School of Medicine, University of Manchester and Wythenshawe Hospital, Manchester, UK
    Lancet Infect Dis 3:230-40. 2003
    ..The standards are absolutes, and, therefore, provide a straightforward basis for improving services to patients if they are all implemented...
  5. ncbi request reprint Echinocandins: a new class of antifungal
    David W Denning
    Education and Research Centre, Wythenshawe Hospital, Southmoor Road, Wythenshawe, Manchester M23 9LT, UK
    J Antimicrob Chemother 49:889-91. 2002
  6. ncbi request reprint Sequencing the Aspergillus fumigatus genome
    David W Denning
    School of Medicine, University of Manchester, Manchester, UK
    Lancet Infect Dis 2:251-3. 2002
    ..Numerous secondary metabolic pathways with biotechnological applications and pharmacological properties are found in the Aspergilli and the genome sequence will facilitate research in this area...
  7. ncbi request reprint Efficacy and safety of voriconazole in the treatment of acute invasive aspergillosis
    David W Denning
    North Manchester General Hospital, University of Manchester, Manchester, United Kingdom
    Clin Infect Dis 34:563-71. 2002
    ..Voriconazole is efficacious in treating acute IA...
  8. ncbi request reprint Muco-cutaneous retinoid-effects and facial erythema related to the novel triazole antifungal agent voriconazole
    D W Denning
    University of Manchester School of Medicine, Department of Infectious Diseases and Tropical Medicine, Monsall Unit, North Manchester General Hospital, Manchester, UK
    Clin Exp Dermatol 26:648-53. 2001
    ..Serum retinoids were elevated in the three patients in whom they were measured. Voriconazole has the potential for retinoid-like side-effects and facial erythema...
  9. doi request reprint Major variations in Aspergillus fumigatus arising within aspergillomas in chronic pulmonary aspergillosis
    S J Howard
    School of Translational Medicine, Manchester Academic Health Science Centre, University Hospital of South Manchester, University of Manchester, UK
    Mycoses 56:434-41. 2013
    ..Importantly, routine sampling from respiratory specimens proved suboptimal in all cases; azole resistance was missed (Patient 1), cultures were negative (Patient 2) and high-level posaconazole resistance was not detected (Patient 3)...
  10. pmc Susceptibility testing of Aspergillus flavus: inoculum dependence with itraconazole and lack of correlation between susceptibility to amphotericin B in vitro and outcome in vivo
    J Mosquera
    Section of Infectious Diseases, Department of Medicine, Hope Hospital, University of Manchester, United Kingdom
    Antimicrob Agents Chemother 45:1456-62. 2001
    ..0 in a microtiter format, incubated for 48 h with no growth end point. Validation of this methodology requires one or more itraconazole-resistant A. flavus isolates, which have yet to be identified...
  11. ncbi request reprint Activity of micafungin (FK463) against an itraconazole-resistant strain of Aspergillus fumigatus and a strain of Aspergillus terreus demonstrating in vivo resistance to amphotericin B
    P A Warn
    School of Medicine, University of Manchester, Manchester, UK
    J Antimicrob Chemother 51:913-9. 2003
    ..terreus infection. No treatment regime was able to totally clear the liver or kidneys in either model. The data indicate that FK463 may have a clinical role in the treatment of life-threatening invasive aspergillosis...
  12. pmc Efficacy of isavuconazole, voriconazole and fluconazole in temporarily neutropenic murine models of disseminated Candida tropicalis and Candida krusei
    J Majithiya
    School of Medicine, University of Manchester, 1 800 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    J Antimicrob Chemother 63:161-6. 2009
    ..The aim of this study was to assess the dose-response of isavuconazole, voriconazole and fluconazole in disseminated Candida tropicalis and Candida krusei infections...
  13. doi request reprint Azole resistance in allergic bronchopulmonary aspergillosis and Aspergillus bronchitis
    S J Howard
    The University of Manchester, The Manchester Academic Health Science Centre, Manchester, UK
    Clin Microbiol Infect 16:683-8. 2010
    ..Both patients responded to posaconazole, with plasma levels of >1.0 mg/L. Subsequently, susceptible strains of different molecular types were cultured from both patients, suggesting eradication and replacement...
  14. doi request reprint High-level expression of cyp51B in azole-resistant clinical Aspergillus fumigatus isolates
    A Buied
    School of Translational Medicine, Manchester Academic Health Science Centre, Faculty of Medicine and Human Sciences, 2nd Floor Education and Research Centre, University Hospital of South Manchester, University of Manchester, Manchester M23 9LT, UK
    J Antimicrob Chemother 68:512-4. 2013
    ..The aim of this study was to investigate possible new mechanisms of resistance in non-target resistance...
  15. ncbi request reprint Flucytosine therapeutic monitoring: 15 years experience from the UK
    A C Pasqualotto
    School of Medicine, The University of Manchester and Wythenshawe Hospital, Manchester, UK
    J Antimicrob Chemother 59:791-3. 2007
    ..There is uniform consensus that flucytosine blood concentrations should be measured to avoid toxicity and ensure adequate efficacy...
  16. ncbi request reprint Atypical eumycetoma caused by Phialophora parasitica successfully treated with itraconazole and flucytosine
    S V Hood
    Department of Infectious Diseases and Tropical Medicine, North Manchester General Hospital, UK
    Br J Dermatol 136:953-6. 1997
    ..She failed to respond to surgical excision and itraconazole, 400 mg daily, but responded to itraconazole, 400 mg daily, and flucytosine, 1 g three times daily, for 12 months. In vitro susceptibility testing predicted a response...
  17. doi request reprint Voriconazole plasma monitoring
    A C Pasqualotto
    Alessandro Pasqualotto, Education and Research Centre, Wythenshawe Hospital, Southmoor Road, Manchester, M23 9LT, UK
    Arch Dis Child 93:578-81. 2008
    ..The purpose of this study was to report the cases of five paediatric patients treated with voriconazole, in which plasma levels were used to monitor therapy...
  18. pmc Mannose-binding lectin genotype and serum levels in patients with chronic and allergic pulmonary aspergillosis
    E Harrison
    The National Aspergillosis Centre, The University Hospital of South Manchester, Manchester Academic Health Science Centre, The University of Manchester, Manchester, UK
    Int J Immunogenet 39:224-32. 2012
    ..023; P = 0.034). Bronchiectasis was not associated with MBL levels in CPA or allergic aspergillosis. MBL genotype and serum level modulate progression of aspergillosis...
  19. ncbi request reprint Cytokine profiling of pulmonary aspergillosis
    H Sambatakou
    The School of Medicine, The University of Manchester, Oxford Road, Manchester, UK
    Int J Immunogenet 33:297-302. 2006
    ..CCPA occurs in patients who are genetically lower producers of both IL-10 and TGF-beta1. As these cytokines are regulatory and anti-inflammatory, CCPA may be a consequence of poor inflammatory response control in the lung...
  20. doi request reprint New and emerging treatments for fungal infections
    A C Pasqualotto
    Education and Research Centre, School of Medicine, The University of Manchester and Wythenshawe Hospital, Southmoor Road, Manchester M23 9LT, UK
    J Antimicrob Chemother 61:i19-30. 2008
    ..Licensure and determination of their place in clinical practice requires randomized clinical studies, which are or will be underway...
  21. ncbi request reprint Treatment of Absidia corymbifera infection in mice with amphotericin B and itraconazole
    J Mosquera
    School of Medicine, Section of Infectious Diseases, University of Manchester, Hope Hospital, Salford, Manchester M6 8HD, UK
    J Antimicrob Chemother 48:583-6. 2001
    ..Amphotericin B MICs were 0.25 mg/L for both strains and itraconazole MICs were 1 and 2 mg/L. Amphotericin B was effective in vivo with both isolates. Itraconazole was less effective...
  22. ncbi request reprint Mannose-binding lectin gene polymorphisms as a susceptibility factor for chronic necrotizing pulmonary aspergillosis
    D J Crosdale
    Arthritis Research Campaign Epidemiology Unit, University of Manchester, Manchester, United Kingdom
    J Infect Dis 184:653-6. 2001
    ..6% of the white control subjects (P=.004). Presence of the codon 52 mutation was particularly common in patients with CNPA (P=.015), which suggests a greater involvement of this mutation...
  23. ncbi request reprint In vitro interaction of terbinafine with itraconazole, fluconazole, amphotericin B and 5-flucytosine against Aspergillus spp
    J Mosquera
    School of Medicine, University of Manchester, Hope Hospital, Salford, Manchester M6 8HD, UK
    J Antimicrob Chemother 50:189-94. 2002
    ..5). FFCs were generally in accord with FICs. The use of terbinafine in combination therapy for Aspergillus infections with azoles seems promising, whereas terbinafine and amphotericin B or flucytosine in combination were less effective...
  24. ncbi request reprint Chronic forms of pulmonary aspergillosis
    D W Denning
    Department of Infectious Diseases and Tropical Medicine, North Manchester General Hospital, UK
    Clin Microbiol Infect 7:25-31. 2001
    ..Surgery is appropriate for isolated aspergillomas, but not pleural or multicavity lesions. Aspergillus empyema is a complication of aspergilloma and CNPA, or surgery for these diseases and is slow to respond to treatment...
  25. ncbi request reprint Molecular genetics in Aspergillus fumigatus
    J L Brookman
    School of Biological Sciences, University of Manchester, 1 800 Stopford Building, Oxford Road, M13 9PT, Manchester, UK
    Curr Opin Microbiol 3:468-74. 2000
    ..fumigatus; for example, the molecular typing of strains during infection. A genome-sequencing programme has begun and will form the basis for future development...
  26. doi request reprint Pathogenicity of Aspergillus fumigatus mutants assessed in Galleria mellonella matches that in mice
    J L Slater
    School of Translational Medicine, The University of Manchester, Manchester Academic Health Sciences Centre, Manchester, UK
    Med Mycol 49:S107-13. 2011
    ..mellonella correlated closely with the historic data generated using mice and Drosophila. Pre-screening Aspergillus mutants using G. mellonella could significantly reduce the number of mammals required to assess changes in virulence...
  27. ncbi request reprint Invasive pulmonary aspergillosis transformed into fatal mucous impaction by immune reconstitution in an AIDS patient
    H Sambatakou
    Education and Research Centre, Wythenshawe University Hospital, Southmoor Road, Manchester, M23 9LT, UK
    Eur J Clin Microbiol Infect Dis 24:628-33. 2005
    ..Invasive pulmonary aspergillosis complicates about 4% of AIDS infections. A search of the medical literature revealed no previous report of this organism's involvement in immune restoration syndrome...
  28. ncbi request reprint An aspergilloma caused by Aspergillus flavus
    A C Pasqualotto
    School of Medicine, The University of Manchester and Wythenshawe Hospital, The Regional Mycology Laboratory, Manchester, UK
    Med Mycol 46:275-8. 2008
    ..Almost all cases are caused by Aspergillus fumigatus. Here we report a patient with an aspergilloma which had Aspergillus flavus recovered from a surgical specimen and serum containing detectable precipitating antibody...
  29. doi request reprint Mixed mould species in laboratory cultures of respiratory specimens: how should they be reported, and what are the indications for susceptibility testing?
    J Purcell
    Department of Microbiology, Clinical Sciences Building, University Hospital of South Manchester, Wythenshawe Hospital, Manchester, UK
    J Clin Pathol 64:543-5. 2011
    ..To investigate how clinical microbiology laboratories should report and interpret mixed mould isolates including Aspergillus species from clinical samples and the criteria for susceptibility testing of the isolates...
  30. ncbi request reprint Infrared body temperature measurement of mice as an early predictor of death in experimental fungal infections
    P A Warn
    School of Medicine, University of Manchester, Hope Hospital, Salford, UK
    Lab Anim 37:126-31. 2003
    ..Adoption of a 33.3 degrees C endpoint in fungal sepsis experiments measured by infrared non-contact thermometer would significantly reduce the suffering in the terminal stages of this type of infection model...
  31. pmc Azole cross-resistance in Aspergillus fumigatus
    J Mosquera
    School of Medicine, University of Manchester, Manchester M13 9PT, United Kingdom
    Antimicrob Agents Chemother 46:556-7. 2002
    ..A complex pattern of cross-resistance and hypersusceptibility was seen with voriconazole and ravuconazole, suggesting marked differences in activity and mechanisms of resistance...
  32. doi request reprint Performance of two Aspergillus IgG EIA assays compared with the precipitin test in chronic and allergic aspergillosis
    C G Baxter
    The National Aspergillosis Centre, University Hospital of South Manchester, Manchester, UK
    Clin Microbiol Infect 19:E197-204. 2013
    ..Both ImmunoCap and Platelia Aspergillus IgG EIAs are sensitive measures of Aspergillus IgG antibodies compared with CIE. However, ImmunoCap appears to have better reproducibility and may be more suitable for monitoring patient disease...
  33. pmc Audit of laboratory mycology services for the management of patients with fungal infections in the northwest of England
    I A Hassan
    Department of Microbiology CSB, SMUHT Wythenshawe Hospital, Manchester, UK
    J Clin Pathol 59:759-63. 2006
    ..Little information exists on laboratory services available and the methods used by general microbiology laboratories to diagnose these important infections...
  34. ncbi request reprint Post-operative aspergillosis
    A C Pasqualotto
    School of Medicine, The University of Manchester and Wythenshawe Hospital, Manchester, UK
    Clin Microbiol Infect 12:1060-76. 2006
    ..In order to improve the outcome, better diagnostic methods are needed, particularly for cases of endocarditis and aortitis...
  35. doi request reprint Underlying conditions in chronic pulmonary aspergillosis including simple aspergilloma
    N L Smith
    The School of Translational Medicine, University of Manchester, Manchester Academic Health Science Centre and University Hospital of South Manchester, Manchester, UK
    Eur Respir J 37:865-72. 2011
    ..Many patients have multiple underlying pulmonary conditions. CPA should be considered when upper lobe cavitary or fibrotic disease and systemic symptoms are present in those with lung disease...
  36. doi request reprint Excess mortality, length of stay and cost attributable to candidaemia
    I Hassan
    Department of Microbiology, University Hospital of South Manchester, Southmoor Road, Manchester, UK
    J Infect 59:360-5. 2009
    ..Such infections are particularly common in the intensive care unit (ICU). The impact of candidaemia on mortality, length of stay (LOS) and cost in a UK hospital was examined...
  37. pmc CADRE: the Central Aspergillus Data REpository
    J E Mabey
    School of Medicine, University of Manchester, Manchester M13 9PT, UK
    Nucleic Acids Res 32:D401-5. 2004
    ..CADRE is accessible at http://www.cadre. man.ac.uk...
  38. ncbi request reprint Sub-cutaneous phaeohyphomycosis caused by Cladophialophora devriesii in a United Kingdom resident
    S J Howard
    Regional Mycology Laboratory, Hope Hospital, Manchester, UK
    Med Mycol 44:553-6. 2006
    ..To date, C. devriesii has been a relatively rare cause of human disease. To our knowledge, this is only the second case to be described, and the first report of infection in a UK resident...
  39. ncbi request reprint Laboratory diagnosis of invasive aspergillosis
    W W Hope
    School of Medicine, University of Manchester and Wythenshawe Hospital, Manchester, UK
    Lancet Infect Dis 5:609-22. 2005
    ..This review considers the application, utility, and limitations of the currently available and investigational diagnostic modalities for invasive aspergillosis...
  40. ncbi request reprint Fungal contamination of bedding
    A A Woodcock
    North West Lung Centre, Wythenshawe Hospital and University of Manchester, Manchester, UK
    Allergy 61:140-2. 2006
    ..It is currently believed that most fungal exposure occurs external to the home...
  41. ncbi request reprint The link between fungi and severe asthma: a summary of the evidence
    D W Denning
    School of Medicine, Mancester University, and Department of Cardiothoracic Medicine, Wythenshawe Hospital, Southmoor Rd, Manchester M23 9LT, UK
    Eur Respir J 27:615-26. 2006
    ..The current authors propose the term "severe asthma with fungal sensitisation". However, it is recognised that enhanced and precise definition of fungal sensitisation will require improvements in diagnostic testing...
  42. ncbi request reprint Generic substitution of itraconazole resulting in sub-therapeutic levels and resistance
    Alessandro Comaru Pasqualotto
    Int J Antimicrob Agents 30:93-4. 2007
  43. ncbi request reprint Voriconazole treatment for subacute invasive and chronic pulmonary aspergillosis
    Helen Sambatakou
    Department of Medicine and Infectious Diseases, University of Athens, Greece
    Am J Med 119:527.e17-24. 2006
    ..We conducted an open, noncomparative multicenter study to evaluate the efficacy and safety of voriconazole in subacute invasive and chronic pulmonary aspergillosis (CPA)...
  44. pmc Molecular mechanisms of primary resistance to flucytosine in Candida albicans
    William W Hope
    Immunocompromised Host Section POB, NCI, NIH CRC, Room 1 5700, 10 Center Dr, MSC 1100, Bethesda, MD 20892 1100, USA
    Antimicrob Agents Chemother 48:4377-86. 2004
    ..A single resistant isolate, lacking the above polymorphism in FUR1, has a homozygous polymorphism in FCA1 that results in a glycine-to-aspartate substitution at position 28 in cytosine deaminase...
  45. ncbi request reprint Imaging findings in acute invasive pulmonary aspergillosis: clinical significance of the halo sign
    Reginald E Greene
    Massachusetts General Hospital, Boston, MA 02114, USA
    Clin Infect Dis 44:373-9. 2007
    ....
  46. pmc Derivation of an in vivo drug exposure breakpoint for flucytosine against Candida albicans and Impact of the MIC, growth rate, and resistance genotype on the antifungal effect
    William W Hope
    Pediatric Oncology Branch, NCI NIH, CRC Rm 1 5750, 10 Center Dr, MSC 1100, Bethesda MD 20892 1100, USA
    Antimicrob Agents Chemother 50:3680-8. 2006
    ..The in vivo growth rate was a critical additional determinant of the exposure-response relationship. There was a relationship between the 5FC resistance genotype and the exposure-response relationship...
  47. doi request reprint Treatment of aspergillosis: clinical practice guidelines of the Infectious Diseases Society of America
    Thomas J Walsh
    Pediatric Oncology Branch, National Cancer Institute, Bethesda, Maryland, USA
    Clin Infect Dis 46:327-60. 2008
  48. pmc Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus
    Natalie D Fedorova
    The J Craig Venter Institute, Rockville, Maryland, United States of America
    PLoS Genet 4:e1000046. 2008
    ..The role of duplication in the origin of lineage-specific genes is further underlined by the discovery of genomic islands that seem to function as designated "gene dumps" and, perhaps, simultaneously, as "gene factories"...
  49. ncbi request reprint Acute pulmonary aspergillosis in immunocompetent subjects after exposure to bark chippings
    Maiken Cavling Arendrup
    Unit of Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark
    Scand J Infect Dis 38:945-9. 2006
    ..One patient with a fatal outcome was initially diagnosed as allergic alveolitis rather than infection and received steroid treatment illustrating the obvious challenges in the differential diagnosis between these disease entities...
  50. ncbi request reprint Comparison of 2 studies of treatment of invasive aspergillosis
    David W Denning
    Clin Infect Dis 45:1106-8; author reply 1108-10. 2007
  51. ncbi request reprint Multi-azole resistance in Aspergillus fumigatus
    Susan J Howard
    School of Medicine, The University of Manchester, Oxford Road, Manchester M13 9PL, UK
    Int J Antimicrob Agents 28:450-3. 2006
    ..The patient had some response to intravenous caspofungin, which he received six times weekly, without the development of resistance over 9 months...
  52. pmc Optimization of the dosage of flucytosine in combination with amphotericin B for disseminated candidiasis: a pharmacodynamic rationale for reduced dosing
    William W Hope
    Department of Medicine, The University of Manchester, 1 800 Stopford Building, Oxford Road, Manchester, UK
    Antimicrob Agents Chemother 51:3760-2. 2007
    ..Here, we bridge the results of an experimental pharmacodynamic study to humans and demonstrate that a 5FC dosage of 25 mg/kg of body weight/day in four divided doses in combination with amphotericin B produces near-maximal effect...
  53. ncbi request reprint Comparative in vivo activity of BAL4815, the active component of the prodrug BAL8557, in a neutropenic murine model of disseminated Aspergillus flavus
    Peter A Warn
    School of Medicine, University of Manchester, 1 800 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    J Antimicrob Chemother 58:1198-207. 2006
    ..We compared the activity of oral BAL8557 with oral itraconazole, oral voriconazole and intravenous caspofungin in a temporarily neutropenic murine model of disseminated Aspergillus flavus...
  54. pmc Effect of neutropenia and treatment delay on the response to antifungal agents in experimental disseminated candidiasis
    William W Hope
    Pediatric Oncology Branch, NCI NIH, CRC Room 1 5750, 10 Center Dr, MSC 1100, Bethesda, MD 20892 1100, USA
    Antimicrob Agents Chemother 51:285-95. 2007
    ..Neutrophils and the timely initiation of antifungal agents are critical determinants in the treatment of experimental disseminated candidiasis...
  55. pmc Does one voriconazole breakpoint suit all Candida species?
    Maiken Cavling Arendrup
    J Clin Microbiol 45:2093; author reply 2094. 2007
  56. pmc Statistical analyses of correlation between fluconazole MICs for Candida spp. assessed by standard methods set forth by the European Committee on Antimicrobial Susceptibility Testing (E.Dis. 7.1) and CLSI (M27-A2)
    Juan Luis Rodriguez-Tudela
    Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Ctra Majadahonda Pozuelo km 2, 28220 Majadahonda, Madrid, Spain
    J Clin Microbiol 45:109-11. 2007
    ..In summary, both methods produce very similar MICs, indicating that methodology does not pose any obstacle to obtaining uniform standards for antifungal susceptibility testing of yeasts...
  57. ncbi request reprint Micafungin (FK463), alone or in combination with other systemic antifungal agents, for the treatment of acute invasive aspergillosis
    David W Denning
    Education and Research Centre, Wythenshawe Hospital and University of Manchester, Academic Department of Medicine and Surgery, Southmoor Road, Manchester M23 9LT, UK
    J Infect 53:337-49. 2006
    ..This study evaluated the safety and efficacy of micafungin in patients with proven or probable invasive aspergillosis (IA)...
  58. ncbi request reprint Genomics of Aspergillus fumigatus
    Catherine M Ronning
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Rev Iberoam Micol 22:223-8. 2005
    ..A. fumigatus was compared with the food biotechnology fungus Aspergillus oryzae and sexual fungus Aspergillus nidulans, as well as other fungi, in an attempt to discern key differences between these organisms...
  59. ncbi request reprint Efficacy and safety of caspofungin for treatment of invasive aspergillosis in patients refractory to or intolerant of conventional antifungal therapy
    Johan Maertens
    University Hospital, Gasthuisberg, Leuven, Belgium
    Clin Infect Dis 39:1563-71. 2004
    ..Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised patients. Echinocandins are novel antifungal molecules with in vitro and in vivo activity against Aspergillus species...
  60. ncbi request reprint Adverse reactions to voriconazole
    Aileen E Boyd
    Department of Infectious Diseases and Tropical Medicine, North Manchester General Hospital, Manchester, United Kingdom
    Clin Infect Dis 39:1241-4. 2004
    ..g., hallucinations, hypoglycemia, electrolyte disturbance, and pneumonitis) possibly attributable to high voriconazole concentrations. Measurement of plasma concentrations could be helpful in optimizing voriconazole dosages...
  61. ncbi request reprint Diagnosis of IPA in HIV: the role of the chest X-ray and radiologist
    Uta Zaspel
    Institut fur Radiologie, Charite Universitatsmedizin Berlin, Campus Mitte, Schuhmannstr 20 21, 10117 Berlin, Germany
    Eur Radiol 14:2030-7. 2004
    ..For internists with regular exposure to HIV patients, chest films do not provide information essential for the verification or differentiation of potential IPA...
  62. ncbi request reprint Invasive pulmonary aspergillosis with spontaneous resolution and the diagnostic utility of PCR from tissue specimens
    William W Hope
    Department of Medicine, Education and Research Centre, Wythenshawe Hospital, University of Manchester, Southmoor Road, Manchester M23 9LT, UK
    J Infect 49:136-40. 2004
    ..can occur without profound immunological deficiency. The case also documents the resolution of IPA without specific therapy...
  63. ncbi request reprint Effect of hypoxic conditions on in vitro susceptibility testing of amphotericin B, itraconazole and micafungin against Aspergillus and Candida
    Peter A Warn
    School of Medicine, University of Manchester, Hope Hospital, Salford M6 8HD
    J Antimicrob Chemother 53:743-9. 2004
    ..The aim of this study was to investigate the effect of hypoxic conditions on in vitro susceptibility testing of amphotericin B, itraconazole and micafungin against Aspergillus (four species) and Candida (six species)...
  64. ncbi request reprint Insight into the genome of Aspergillus fumigatus: analysis of a 922 kb region encompassing the nitrate assimilation gene cluster
    Arnab Pain
    The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Fungal Genet Biol 41:443-53. 2004
    ..We observed large scale synteny between A. fumigatus and Aspergillus nidulans by comparing this sequence to the A. nidulans genetic map of linkage group VIII...
  65. pmc Interlaboratory evaluation of hematocytometer method of inoculum preparation for testing antifungal susceptibilities of filamentous fungi
    J L Rodriguez-Tudela
    Unidad de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Spain
    J Clin Microbiol 41:5236-7. 2003
    ..The agreement and correlation coefficient between conidium and colony quantifications were 89.2% and 0.73 (P < 0.01). Correlations with optical density determination were not significant...
  66. ncbi request reprint Increased expression of a novel Aspergillus fumigatus ABC transporter gene, atrF, in the presence of itraconazole in an itraconazole resistant clinical isolate
    John W Slaven
    School of Medicine, 1 800 Stopford Building, University of Manchester, Oxford Road, Manchester, UK
    Fungal Genet Biol 36:199-206. 2002
    ..atrF is the first ABCT gene cloned from A. fumigatus, whose overexpression is correlated with itraconazole resistance...
  67. ncbi request reprint Combination and sequential antifungal therapy for invasive aspergillosis: review of published in vitro and in vivo interactions and 6281 clinical cases from 1966 to 2001
    William J Steinbach
    Division of Pediatric Infectious Diseases, Department of Pediatrics, Duke University, Durham, North Carolina, USA
    Clin Infect Dis 37:S188-224. 2003
    ..Improvement was noted with amphotericin B or itraconazole followed by voriconazole but not with itraconazole followed by amphotericin B...
  68. ncbi request reprint Advances against aspergillosis
    William J Steinbach
    Division of Pediatric Infectious Diseases, Department of Pediatrics, Duke University, Durham, North Carolina, USA
    Clin Infect Dis 37:S155-6. 2003
  69. ncbi request reprint Comparison of three methods for in vitro susceptibility testing of Candida species with flucytosine
    Caroline B Moore
    Department of Microbiology, Section of Infectious Diseases, Department of Medicine, Hope Hospital, Eccles Old Road, Salford M6 8HD
    J Antimicrob Chemother 51:297-304. 2003
    ..The methodology for susceptibility testing C. albicans is robust. Additional work to optimize susceptibility testing with flucytosine is necessary for non-albicans Candida species, especially C. krusei...
  70. ncbi request reprint In vivo activity of micafungin in a persistently neutropenic murine model of disseminated infection caused by Candida tropicalis
    Peter A Warn
    School of Medicine, University of Manchester, UK
    J Antimicrob Chemother 50:1071-4. 2002
    ..tropicalis. Micafungin was well tolerated by the mice and was much more effective than amphotericin B or fluconazole against an amphotericin B- and fluconazole-resistant C. tropicalis...
  71. ncbi request reprint Advances against aspergillosis (September 9-11, 2004, San Francisco, California)
    Karl V Clemons
    Department of Medicine, Santa Clara Valley Medical Center, 751 So Bascom Av, San Jose, California 95128 2699, USA
    Mycopathologia 159:315-7. 2005
  72. ncbi request reprint Voriconazole versus amphotericin B for primary therapy of invasive aspergillosis
    Raoul Herbrecht
    Département d Hématologie et d Oncologie, Hopital de Hautepierre, Strasbourg, France
    N Engl J Med 347:408-15. 2002
    ..Voriconazole is a broad-spectrum triazole that is active against aspergillus species. We conducted a randomized trial to compare voriconazole with amphotericin B for primary therapy of invasive aspergillosis...
  73. ncbi request reprint Strategy of following voriconazole versus amphotericin B therapy with other licensed antifungal therapy for primary treatment of invasive aspergillosis: impact of other therapies on outcome
    Thomas F Patterson
    Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229 3900, USA
    Clin Infect Dis 41:1448-52. 2005
    ..In that trial, treatment with voriconazole or amphotericin B deoxycholate could be followed with other licensed antifungal therapies (OLAT). Here, we report the impact of OLAT on the outcome of patients with invasive aspergillosis...
  74. ncbi request reprint In vitro activity of a new triazole BAL4815, the active component of BAL8557 (the water-soluble prodrug), against Aspergillus spp
    Peter A Warn
    School of Medicine, 1 800 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    J Antimicrob Chemother 57:135-8. 2006
    ....
  75. ncbi request reprint Activity of aminocandin (IP960) compared with amphotericin B and fluconazole in a neutropenic murine model of disseminated infection caused by a fluconazole-resistant strain of Candida tropicalis
    Peter A Warn
    School of Medicine, 1 800 Stopford Building, University of Manchester, Manchester M13 9PT, UK
    J Antimicrob Chemother 56:590-3. 2005
    ..with that of amphotericin B and fluconazole in a temporarily immunocompromised murine model of disseminated candidiasis...
  76. ncbi request reprint Surface response modeling to examine the combination of amphotericin B deoxycholate and 5-fluorocytosine for treatment of invasive candidiasis
    William W Hope
    Department of Medicine, University of Manchester, Manchester, UK
    J Infect Dis 192:673-80. 2005
    ..Our methods provide a way in which some of the complex issues surrounding antifungal combination treatment can be addressed...
  77. ncbi request reprint The efficacy and tolerability of voriconazole in the treatment of chronic cavitary pulmonary aspergillosis
    Lakshmi R Jain
    School of Medicine, The University of Manchester, Wythenshawe Hospital, Manchester, UK
    J Infect 52:e133-7. 2006
    ..Voriconazole is a useful alternative therapy for CCPA, with a response rate of 64%, over 3 months, and continuing partial remission of disease for much longer periods...
  78. ncbi request reprint Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae
    James E Galagan
    The Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, Massachusetts 02142, USA
    Nature 438:1105-15. 2005
    ..These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation...
  79. ncbi request reprint Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus
    William C Nierman
    The Institute for Genomic Research, Rockville, Maryland 20850, USA
    Nature 438:1151-6. 2005
    ..The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus...
  80. ncbi request reprint Allergic bronchopulmonary aspergillosis in cystic fibrosis--state of the art: Cystic Fibrosis Foundation Consensus Conference
    David A Stevens
    Department of Medicine, Santa Clara Valley Medical Center, Stanford University Medical School, San Jose, California 95128 2699, USA
    Clin Infect Dis 37:S225-64. 2003
    ..Diagnostic criteria that could provide a framework for monitoring were adopted, and helpful imaging features were identified. New possibilities in therapy produced plans for managing diverse clinical presentations...
  81. ncbi request reprint Clinical research in the lay press: irresponsible journalism raises a huge dose of doubt
    Elias J Anaissie
    Myeloma Institute for Research and Therapy, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Clin Infect Dis 43:1031-9. 2006
  82. ncbi request reprint Genome sequencing and analysis of Aspergillus oryzae
    Masayuki Machida
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Nature 438:1157-61. 2005
    ..Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation...
  83. ncbi request reprint Candida glabrata STE12 is required for wild-type levels of virulence and nitrogen starvation induced filamentation
    Ana Maria Calcagno
    Department of Infectious Diseases and Microbiology, Imperial College London, Du Cane Road, London, W12 0NN, UK
    Mol Microbiol 50:1309-18. 2003
    ..Candida glabrata STE12 is therefore the first virulence factor encoding gene to be described in this increasingly important fungal pathogen...
  84. pmc Multilocus sequence typing of Candida glabrata reveals geographically enriched clades
    Andrew R Dodgson
    School of Medicine, University of Manchester, Manchester, United Kingdom
    J Clin Microbiol 41:5709-17. 2003
    ..Our data demonstrate for the first time, with such a large geographically diverse strain collection, that distinct genetic clades of C. glabrata prevail in different geographical regions...
  85. pmc Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) C
    Ben de Pauw
    Department of Blood Transfusion Service and Transplant Immunology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Infect Dis 46:1813-21. 2008
    ..Clarity and uniformity in defining these infections are important factors in improving the quality of clinical studies. A standard set of definitions strengthens the consistency and reproducibility of such studies...
  86. ncbi request reprint Genomic analysis of allergen genes in Aspergillus spp: the relevance of genomics to everyday research
    Paul Bowyer
    Faculty of Medicine and Human Sciences, University of Manchester, Wythenshawe Hospital, Manchester, UK
    Med Mycol 45:17-26. 2007
    ..We also analysed allergens in the 3 sequenced aspergilli to look for internal homologies and this suggests that multi gene families may produce numerous cross-reactive allergens...
  87. ncbi request reprint Evidence for sexuality in the opportunistic fungal pathogen Aspergillus fumigatus
    Mathieu Paoletti
    School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
    Curr Biol 15:1242-8. 2005
    ..The possible presence of a sexual cycle is highly significant for the population biology and disease management of the species...
  88. ncbi request reprint Validation of an assay for voriconazole in serum samples using liquid chromatography-tandem mass spectrometry
    Brian G Keevil
    Department of Clinical Biochemistry, Wythenshawe Hospital, South Manchester University Hospitals, NHS Trust, Manchester M23 9LT, England, UK
    Ther Drug Monit 26:650-7. 2004
    ..38 to 15.3 mg/L. The lower limit of quantification was 0.1 mg/L. Regression analysis showed HPLC-MS/MS = 1.06 +/- 0.02 (HPLC-UV) - 0.07 +/- 0.1, R2 = 0.95, n = 99...
  89. ncbi request reprint Aspergillosis in "nonimmunocompromised" critically ill patients
    David W Denning
    Am J Respir Crit Care Med 170:580-1. 2004
  90. ncbi request reprint Correlation between in vitro growth rate and in vivo virulence in Aspergillus fumigatus
    Derek Paisley
    Clinical Sciences Building, Hope Hospital, Salford, UK
    Med Mycol 43:397-401. 2005
    ..fumigatus isolates for which an LD90 value in immunosuppressed CD-1 mice had previously been obtained. Comparison of the growth rates and LD90 values of these isolates suggests that a correlation exists between the two parameters...
  91. pmc Inactivation of transcription factor gene ACE2 in the fungal pathogen Candida glabrata results in hypervirulence
    Mohammed Kamran
    Department of Infectious Diseases, Imperial College London, London
    Eukaryot Cell 3:546-52. 2004
    ..These data demonstrate that C. glabrata ACE2 encodes a function that plays a critical role in mediating the host-Candida interaction. It is the first virulence-moderating gene to be described for a Candida species...
  92. pmc Comparative genomics of fungal allergens and epitopes shows widespread distribution of closely related allergen and epitope orthologues
    Paul Bowyer
    Faculty of Medicine and Human Sciences, University of Manchester, Wythenshawe Hospital, Manchester, M23 9LT, UK
    BMC Genomics 7:251. 2006
    ..Here we use comparative genomics to survey the occurrence of allergen orthologues in fungi...
  93. ncbi request reprint Comparison of extracellular phospholipase activities in clinical and environmental Aspergillus fumigatus isolates
    Mike Birch
    University of Manchester, Department of Medicine, Hope Hospital, Salford, UK
    Med Mycol 42:81-6. 2004
    ..fumigatus. This study suggests that extracellular phospholipase C activity, but not extracellular acyl hydrolase activity may be important in the pathogenicity of A. fumigatus...
  94. pmc A cautionary tale: Lack of consistency in allele sizes between two laboratories for a published multilocus microsatellite typing system
    Alessandro C Pasqualotto
    School of Medicine, The University of Manchester, Manchester, UK
    J Clin Microbiol 45:522-8. 2007
    ..This study highlights the care required to ensure that an MLMT system undergoes a suitable correction procedure before data can be merged between different laboratories involved in the typing of individual species...