Chris Denning

Summary

Affiliation: University of Nottingham
Country: UK

Publications

  1. ncbi request reprint Evaluating the utility of cardiomyocytes from human pluripotent stem cells for drug screening
    Emily Dick
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Biochem Soc Trans 38:1037-45. 2010
  2. pmc Drug evaluation in cardiomyocytes derived from human induced pluripotent stem cells carrying a long QT syndrome type 2 mutation
    Elena Matsa
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, University of Nottingham, Nottingham NG7 2RD, UK
    Eur Heart J 32:952-62. 2011
  3. pmc Current status of drug screening and disease modelling in human pluripotent stem cells
    Divya Rajamohan
    Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, UK
    Bioessays 35:281-98. 2013
  4. ncbi request reprint New frontiers in gene targeting and cloning: success, application and challenges in domestic animals and human embryonic stem cells
    Chris Denning
    University of Nottingham, Division of Animal Physiology, School of Biosciences, Sutton Bonington LE12 5RD, UK
    Reproduction 126:1-11. 2003
  5. ncbi request reprint Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7
    Chris Denning
    Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, UK
    Int J Dev Biol 50:27-37. 2006
  6. doi request reprint Two new protocols to enhance the production and isolation of human induced pluripotent stem cell lines
    Emily Dick
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Stem Cell Res 6:158-67. 2011
  7. doi request reprint Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy
    Flavius C Pascut
    School of Physics and Astronomy, University of Nottingham, Nottingham, NG7 2RD, UK
    Biochim Biophys Acta 1830:3517-24. 2013
  8. ncbi request reprint In vitro uses of human pluripotent stem cell-derived cardiomyocytes
    Elena Matsa
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, University of Nottingham, University Park, Nottingham, UK
    J Cardiovasc Transl Res 5:581-92. 2012
  9. pmc Exon skipping and gene transfer restore dystrophin expression in human induced pluripotent stem cells-cardiomyocytes harboring DMD mutations
    Emily Dick
    1 Wolfson Centre for Stem Cells, Tissue Engineering and Modelling STEM, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom
    Stem Cells Dev 22:2714-24. 2013
  10. doi request reprint Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells
    Flavius C Pascut
    University of Nottingham, School of Physics and Astronomy, Nottingham NG7 2RD, United Kingdom
    J Biomed Opt 16:045002. 2011

Collaborators

Detail Information

Publications31

  1. ncbi request reprint Evaluating the utility of cardiomyocytes from human pluripotent stem cells for drug screening
    Emily Dick
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, UK
    Biochem Soc Trans 38:1037-45. 2010
    ..This suggests that further development and validation of the hPSC-cardiomyocyte model as a tool for assessing cardiotoxicity is warranted...
  2. pmc Drug evaluation in cardiomyocytes derived from human induced pluripotent stem cells carrying a long QT syndrome type 2 mutation
    Elena Matsa
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, University of Nottingham, Nottingham NG7 2RD, UK
    Eur Heart J 32:952-62. 2011
    ..Limitations to existing clinical therapeutic management strategies prompted us to develop a novel human in vitro drug-evaluation system for LQTS type 2 (LQT2) that will complement the existing in vitro and in vivo models...
  3. pmc Current status of drug screening and disease modelling in human pluripotent stem cells
    Divya Rajamohan
    Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, UK
    Bioessays 35:281-98. 2013
    ..Such successes will provide an incentive to overcome bottlenecks in hPSC technology such as improving cell maturity and industrial scalability whilst reducing cost...
  4. ncbi request reprint New frontiers in gene targeting and cloning: success, application and challenges in domestic animals and human embryonic stem cells
    Chris Denning
    University of Nottingham, Division of Animal Physiology, School of Biosciences, Sutton Bonington LE12 5RD, UK
    Reproduction 126:1-11. 2003
    ..This review will discuss some of the successes, applications and challenges facing gene targeting in livestock and hES cells...
  5. ncbi request reprint Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7
    Chris Denning
    Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, UK
    Int J Dev Biol 50:27-37. 2006
    ..Therefore the same culture and differentiation conditions functioned in two independently-derived hESC lines. Similar studies in other lines may facilitate development of universal protocols...
  6. doi request reprint Two new protocols to enhance the production and isolation of human induced pluripotent stem cell lines
    Emily Dick
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Stem Cell Res 6:158-67. 2011
    ..We have used these techniques to produce 8 hiPSC lines from human fibroblasts and we believe that they are of considerable utility to researchers in the hiPSC field...
  7. doi request reprint Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy
    Flavius C Pascut
    School of Physics and Astronomy, University of Nottingham, Nottingham, NG7 2RD, UK
    Biochim Biophys Acta 1830:3517-24. 2013
    ..In this paper we report the use of Raman micro-spectroscopy (RMS) to measure time- and spatially-resolved molecular changes in intact embryoid bodies (EBs) during in-vitro cardiogenic differentiation...
  8. ncbi request reprint In vitro uses of human pluripotent stem cell-derived cardiomyocytes
    Elena Matsa
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, University of Nottingham, University Park, Nottingham, UK
    J Cardiovasc Transl Res 5:581-92. 2012
    ....
  9. pmc Exon skipping and gene transfer restore dystrophin expression in human induced pluripotent stem cells-cardiomyocytes harboring DMD mutations
    Emily Dick
    1 Wolfson Centre for Stem Cells, Tissue Engineering and Modelling STEM, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom
    Stem Cells Dev 22:2714-24. 2013
    ..This demonstrates that DMD hiPSC-cardiomyocytes provide a novel tool to evaluate whether new therapeutics can restore dystrophin expression in the heart. ..
  10. doi request reprint Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells
    Flavius C Pascut
    University of Nottingham, School of Physics and Astronomy, Nottingham NG7 2RD, United Kingdom
    J Biomed Opt 16:045002. 2011
    ..This would start to progress RMS to the field of cell sorting for applications such as enrichment and purification of hESC-derived cardiomyocytes...
  11. ncbi request reprint Molecular and phenotypic analyses of human embryonic stem cell-derived cardiomyocytes: opportunities and challenges for clinical translation
    Gareth Goh
    Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, UK
    Thromb Haemost 94:728-37. 2005
    ..This review evaluates the opportunities presented by hESC-derived cardiomyocytes and the progress towards surmounting the challenges of clinical translation...
  12. ncbi request reprint Improved human embryonic stem cell embryoid body homogeneity and cardiomyocyte differentiation from a novel V-96 plate aggregation system highlights interline variability
    Paul W Burridge
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Human Development, University of Nottingham, Queen s Medical Centre, Nottingham, United Kingdom
    Stem Cells 25:929-38. 2007
    ..Thus, the V-96FA system highlighted interline variability in EB growth and cardiomyocyte differentiation but, under the test conditions described, identified HUES-7 as a line that can respond to cardiomyogenic stimulation...
  13. pmc Directed differentiation of human embryonic stem cells to interrogate the cardiac gene regulatory network
    James E Dixon
    School of Pharmacy, Wolfson Centre for Stem Cells, Tissue Engineering, and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, UK
    Mol Ther 19:1695-703. 2011
    ..This system provides a route to test the effect of different factors on human cardiac differentiation and will be useful in understanding the network failures that underlie disease phenotypes...
  14. doi request reprint 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development
    Rimple D Almeida
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling STEM, School of Clinical Sciences, University of Nottingham, Nottingham, UK
    Epigenetics 7:383-9. 2012
    ..Our findings show that 5-hmC enrichment of non-committed cells is not a universal feature of vertebrate development and give insights both into evolution of embryonic pluripotency and the potential role of 5-hmC in its regulation...
  15. ncbi request reprint Human embryonic stem cell methyl cycle enzyme expression: modelling epigenetic programming in assisted reproduction?
    William Steele
    Division of Obstetrics and Gynaecology, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK
    Reprod Biomed Online 10:755-66. 2005
    ..These studies indicate that altered methyl group metabolism provides a potential mechanism for inducing epigenetic changes in the preimplantation embryo...
  16. ncbi request reprint Materials for stem cell factories of the future
    Adam D Celiz
    1 Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK 2 Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, Massachusetts 02115, USA
    Nat Mater 13:570-9. 2014
    ..In this Review, we consider the role of materials discovery, an emerging area of materials chemistry that is in large part driven by the challenges posed by biologists to materials scientists. ..
  17. ncbi request reprint Stem-cell consequences of embryo epigenetic defects
    Cinzia Allegrucci
    Division of Obstetrics and Gynaecology, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK
    Lancet 364:206-8. 2004
    ....
  18. pmc Gene-specific vulnerability to imprinting variability in human embryonic stem cell lines
    Kee Pyo Kim
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling STEM, University of Nottingham, Centre for Biomolecular Sciences, Nottingham NG7 2RD, United Kingdom
    Genome Res 17:1731-42. 2007
    ..We conclude the prudence of including comprehensive imprinting analysis in the continued characterization of human embryonic stem cell lines...
  19. ncbi request reprint Rapid micropatterning of cell lines and human pluripotent stem cells on elastomeric membranes
    Isha Paik
    Wolfson Centre for Stem Cells, Tissue Engineering, and Modelling STEM, Centre of Biomolecular Sciences, School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
    Biotechnol Bioeng 109:2630-41. 2012
    ..The ability to rapidly pattern cells at high resolution over macro scales should aid future tissue engineering efforts for regenerative medicine applications and in creating in vitro stem cell niches...
  20. doi request reprint Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection
    Emily Dick
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, UK
    Nat Protoc 6:701-14. 2011
    ..Taken together, these methods accelerate the production of hiPSCs and enable lines to be isolated, expanded to approxiamtely 10⁷ cells and cryopreserved within 6-8 weeks...
  21. ncbi request reprint Transgenic enrichment of cardiomyocytes from human embryonic stem cells
    David Anderson
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, University of Nottingham, Nottingham, UK
    Mol Ther 15:2027-36. 2007
    ..Both transgenic strategies will be generically applicable and should readily translate to the enrichment of many other differentiated lineages derived from hESCs...
  22. pmc Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation
    James E Dixon
    Wolfson Centre for Stem Cells, Tissue Engineering, and Modelling, Centre of Biomolecular Sciences, School of Pharmacy, Medical Engineering Unit, School of Mechanical, Materials and Manufacturing Engineering, Nottingham Nanotechnology and Nanoscience Centre, Faculty of Engineering, and School of Medicine, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    Proc Natl Acad Sci U S A 111:5580-5. 2014
    ..This work will facilitate regenerative medicine by allowing in situ HPSC expansion to be coupled with early lineage specification within defined tissue geometries. ..
  23. pmc Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes
    Elena Matsa
    Wolfson Centre for Stem Cells, Tissue Engineering and Modelling STEM, University of Nottingham, Nottingham NG7 2RD, UK
    Eur Heart J 35:1078-87. 2014
    ..We sought to use cardiomyocytes derived from human-induced pluripotency stem cells (hiPSCs) as an in vitro model to develop and evaluate gene-based therapeutics for the treatment of LQTS...
  24. ncbi request reprint Bioluminescence imaging of human embryonic stem cells transplanted in vivo in murine and chick models
    Helen Priddle
    Division of Human Development, University of Nottingham, Queens Medical Centre, Nottingham, United Kingdom
    Cloning Stem Cells 11:259-67. 2009
    ..These results show the widespread utility of bioluminescent for in vivo imaging of hESCs in a variety of model systems for preclinical research into regenerative medicine and cancer biology...
  25. ncbi request reprint Maintenance of pluripotency in human embryonic stem cells cultured on a synthetic substrate in conditioned medium
    Magdalena M Mahlstedt
    School of Pharmacy, University of Nottingham, Nottingham, UK
    Biotechnol Bioeng 105:130-40. 2010
    ..Generation of cost-effective, easy-to-handle synthetic, defined, stable surfaces for hESC culture will expedite stem cell use in biomedical applications...
  26. pmc Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells
    Flavius C Pascut
    School of Physics and Astronomy, University of Nottingham, Nottingham, UK
    Biophys J 100:251-9. 2011
    ....
  27. ncbi request reprint The patentability of human embryonic stem cells in Europe
    Gerard Porter
    University of Nottingham, School of Law, Nottingham NG7 2RD, UK
    Nat Biotechnol 24:653-5. 2006
    ..Applicants in Europe are left with few options for the patent protection of hES cell-related technology...
  28. ncbi request reprint Genetic modification of sheep by nuclear transfer with gene-targeted somatic cells
    A John Clark
    Roslin Institute, Roslin, Midlothian, UK
    Methods Mol Biol 348:199-212. 2006
    ..This chapter describes protocols for culturing primary sheep fibroblasts, introducing and selecting targeted modifications into them and then using these modified cells in nuclear transfer experiments...
  29. ncbi request reprint Human embryonic stem cells: towards therapies for cardiac disease. Derivation of a Dutch human embryonic stem cell line
    Anja van de Stolpe
    Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Reprod Biomed Online 11:476-85. 2005
    ....
  30. ncbi request reprint Improved genetic manipulation of human embryonic stem cells
    Stefan R Braam
    Hubrecht Institute, Developmental Biology and Stem Cell Research, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nat Methods 5:389-92. 2008
    ..Stably transfected lines retained differentiation potential, and most lines displayed normal karyotypes...
  31. ncbi request reprint Proliferative lifespan is conserved after nuclear transfer
    A John Clark
    Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, UK
    Nat Cell Biol 5:535-8. 2003
    ..The rederived lines had the same proliferative capacity and rate of telomere shortening as the donor cell lines, suggesting that these are innate, genetically determined, properties that are conserved by nuclear transfer...