Chris Darke

Summary

Affiliation: University Hospital of Wales
Country: UK

Publications

  1. doi request reprint One-tube HLA-B27/B2708 typing by flow cytometry using two "Anti-HLA-B27" monoclonal antibody reagents
    Chris Darke
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Wales, United Kingdom
    Cytometry B Clin Cytom 78:21-30. 2010
  2. ncbi request reprint Immunogenetics of two new HLA-B alleles: B*4414 and B*5708
    C Darke
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Wales, UK
    Tissue Antigens 62:436-41. 2003
  3. ncbi request reprint Immunogenetic study of a new HLA allele, B*2723
    C Darke
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, United Kingdom
    Tissue Antigens 60:400-3. 2002
  4. ncbi request reprint Molecular, serological and genetic studies on two new HLA-DRB1 alleles--HLA-DRB1*0704 and HLA-DRB1*1507
    C Darke
    Regional Tissue Typing Laboratory, Welsh Blood Service, Cardiff, United Kingdom
    Tissue Antigens 56:467-9. 2000
  5. ncbi request reprint HLA-A*24020102L in the UK blood donor population
    P P J Dunn
    British Bone Marrow Registry, DNA Reference Laboratory, National Blood Service, Bristol, UK
    Tissue Antigens 63:589-91. 2004
  6. ncbi request reprint Human leucocyte antigen polymorphisms in nut-allergic patients in South Wales
    S Hand
    Department of Medicine, Prince Charles Hospital, North Glamorgan NHS Trust, Merthyr Tydfi, UK
    Clin Exp Allergy 34:720-4. 2004
  7. ncbi request reprint Five-locus HLA typing of hematopoietic stem cell donor volunteers using PCR sequence specific primers
    J Downing
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Cardiff, Wales, UK
    Genet Test 8:301-12. 2004
  8. ncbi request reprint A typing system for the major histocompatibility complex class I chain related genes A and B using polymerase chain reaction with sequence-specific primers
    M T Rees
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun CF72 9WB, Wales, United Kingdom
    Genet Test 9:93-110. 2005
  9. ncbi request reprint Identification of a novel HLA-A allele (A*1115) in the UK National External Quality Assessment Schemes for Histocompatibility and Immunogenetics' Educational Cell Exchange
    N Bendukidze
    H and I Department, National Blood Service, Bristol, UK
    Tissue Antigens 67:153-6. 2006
  10. ncbi request reprint A high-resolution polymerase chain reaction-sequence-specific primer HLA-B*27 typing set and its application in routine HLA-B27 testing
    J Downing
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Wales, United Kingdom
    Genet Test 10:98-103. 2006

Collaborators

Detail Information

Publications29

  1. doi request reprint One-tube HLA-B27/B2708 typing by flow cytometry using two "Anti-HLA-B27" monoclonal antibody reagents
    Chris Darke
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Wales, United Kingdom
    Cytometry B Clin Cytom 78:21-30. 2010
    ..However, the generally used "HLA-B27" monoclonal antibodies (moabs) cross-react with many HLA specificities, including the common HLA-B7 antigen. Thus, using two "B27" moabs is highly recommended...
  2. ncbi request reprint Immunogenetics of two new HLA-B alleles: B*4414 and B*5708
    C Darke
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Wales, UK
    Tissue Antigens 62:436-41. 2003
    ..00002 (carriage frequency 0.004%). B*4414 and B*5708 specificities both gave 'short' serological reactivity for their expected specificities and Bw4. The likely reasons for this are discussed in relation to the epitopes of B44 and B57...
  3. ncbi request reprint Immunogenetic study of a new HLA allele, B*2723
    C Darke
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, United Kingdom
    Tissue Antigens 60:400-3. 2002
    ..Lack of the ECAKA motif at amino acid positions 63, 67, 69-71 probably accounts for lack of the B27 specificity while the amino acid combination 74Y, 77S, 80T, 81L may cause aberrant Bw4 reactivity...
  4. ncbi request reprint Molecular, serological and genetic studies on two new HLA-DRB1 alleles--HLA-DRB1*0704 and HLA-DRB1*1507
    C Darke
    Regional Tissue Typing Laboratory, Welsh Blood Service, Cardiff, United Kingdom
    Tissue Antigens 56:467-9. 2000
    ..No further examples were found in 19,113 HLA-DR,DQ typed donors from the Welsh Bone Marrow Donor Registry indicating that both these alleles have a phenotype frequency of <0.01% and a gene frequency of <0.00003...
  5. ncbi request reprint HLA-A*24020102L in the UK blood donor population
    P P J Dunn
    British Bone Marrow Registry, DNA Reference Laboratory, National Blood Service, Bristol, UK
    Tissue Antigens 63:589-91. 2004
    ..Consequently, HLA-A*24020102L, B*55, Cw*01 is a principal A*24020102L-bearing haplotype, and the minimum carriage and gene frequencies of A*24020102L are 0.017% and 0.000086, respectively, in UK blood donors...
  6. ncbi request reprint Human leucocyte antigen polymorphisms in nut-allergic patients in South Wales
    S Hand
    Department of Medicine, Prince Charles Hospital, North Glamorgan NHS Trust, Merthyr Tydfi, UK
    Clin Exp Allergy 34:720-4. 2004
    ..Peanuts and tree nuts are among the most common foods provoking severe allergic reactions including fatal anaphylaxis. However, little is known of the underlying genetic and immunological mechanisms involved...
  7. ncbi request reprint Five-locus HLA typing of hematopoietic stem cell donor volunteers using PCR sequence specific primers
    J Downing
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Cardiff, Wales, UK
    Genet Test 8:301-12. 2004
    ..This allows resources to be focused directly on allele level typing of DRB1 and other loci. This strategy decreases overall donor work-up time, which is a major benefit to patients...
  8. ncbi request reprint A typing system for the major histocompatibility complex class I chain related genes A and B using polymerase chain reaction with sequence-specific primers
    M T Rees
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun CF72 9WB, Wales, United Kingdom
    Genet Test 9:93-110. 2005
    ..It is easily updated as new alleles are identified but clearly requires a continuing validation review until all known MICA and MICB alleles have been identified...
  9. ncbi request reprint Identification of a novel HLA-A allele (A*1115) in the UK National External Quality Assessment Schemes for Histocompatibility and Immunogenetics' Educational Cell Exchange
    N Bendukidze
    H and I Department, National Blood Service, Bristol, UK
    Tissue Antigens 67:153-6. 2006
    ..Extensive serological typing indicated that this allele essentially encodes a 'normal' HLA-A11 specificity...
  10. ncbi request reprint A high-resolution polymerase chain reaction-sequence-specific primer HLA-B*27 typing set and its application in routine HLA-B27 testing
    J Downing
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Wales, United Kingdom
    Genet Test 10:98-103. 2006
    ..The distribution of B*27 alleles was determined in a random population of 4020 local blood donors and the use of PCR-SSP B*27 typing in our routine flow cytometry-based HLA-B27/B2708 typing strategy is described...
  11. ncbi request reprint HLA-A*2312--a novel allele found during testing for the UK NEQAS for H&I DNA HLA-typing scheme
    L Hammond
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Wales, UK, and Department of Oncology, Radiology and Clinical Immunology, Rudbeck Laboratory, University Hospital, Uppsala, Sweden
    Tissue Antigens 68:175-6. 2006
  12. ncbi request reprint A new HLA-B*5501 variant--B*550104
    L Hammond
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Wales, UK
    Tissue Antigens 68:182-3. 2006
  13. ncbi request reprint A new HLA-Cw*03 variant - Cw*031102
    L Hammond
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Wales, UK
    Tissue Antigens 68:455-6. 2006
  14. ncbi request reprint Non-B27 MHC associations of ankylosing spondylitis
    A M Sims
    Botnar Research Centre, Nuffield Orthopaedic Centre, Oxford, UK
    Genes Immun 8:115-23. 2007
    ..Interrogation of the data identified a region of 270 kb, lying from 31 952 649 to 32 221 738 base pairs from the p-telomere of chromosome 6 and containing 23 genes, which is likely to include genes involved with susceptibility to AS...
  15. ncbi request reprint A new HLA-B*15 variant - B*9507
    L Hammond
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Wales, UK
    Tissue Antigens 70:519-21. 2007
    ..This results in a single amino acid change of serine to arginine (AGC>CGC) at codon 131 and a silent substitution (GCG>GCC - conserved alanine) at codon 135...
  16. ncbi request reprint Provision of Epstein-Barr virus-transformed B-cell lines in a routine tissue typing laboratory: practicalities and applications
    H Bass
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, UK
    Eur J Immunogenet 31:87-92. 2004
    ..Careful adherence to the methods and procedures detailed here should virtually guarantee successful transformation (98.7%) from good quality, sterile whole blood samples...
  17. ncbi request reprint Immunogenetics of a new HLA-B null allele, HLA-B*4423N
    L Hammond
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, UK
    Eur J Immunogenet 30:385-6. 2003
    ..The B*4423N-bearing haplotype was identified as A*0201, Cw*0501, DRB1*0408, DRB4*01, DQA1*03, DQB1*0304 and the frequency of B*4423N estimated as 0.00006 (carriage frequency 0.0121%) in 16 533 subjects resident in Wales...
  18. ncbi request reprint HFE mutations, iron deficiency and overload in 10,500 blood donors
    H A Jackson
    Department of Haematology, University of Wales College of Medicine and University Hospital of Wales, Cardiff, UK
    Br J Haematol 114:474-84. 2001
    ..The Welsh Blood Service collects blood from about 140 000 people each year including an estimated 950 who are homozygous for HFE C282Y. They are probably healthy and unaware of any family history of iron overload...
  19. ncbi request reprint Sequence, genetics and serology of a new HLA-B allele: B*4903
    C Darke
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyklun, United Kingdom
    Tissue Antigens 57:478-80. 2001
    ..No further examples of B*4903 were found in 15,796 consecutive HLA PCR-SSP typed donors from the Welsh Bone Marrow Donor Registry, indicating that this allele has a phenotype frequency of <0.01% and a gene frequency of <0.00004...
  20. ncbi request reprint A new HLA-B44 variant (B44BO [B*4408]) identified by serology
    C Darke
    Regional Tissue Typing Laboratory, Welsh Blood Service, Wales, United Kingdom
    Tissue Antigens 50:32-7. 1997
    ..The sequence and serological reactivity of this rare antigen allowed the identification of two likely epitopes shared by two different groups of HLA-B antigens...
  21. ncbi request reprint Immunogenetics of the rare HLA-B allele B*4408
    J Street
    Welsh Transplantation and Immunology, Welsh Blood Service, Pontyclun, UK
    Eur J Immunogenet 30:129-31. 2003
    ..The phenotype and gene frequencies of B*4408, in a sample of 40 473 subjects residing in Wales, were 0.01235% and 0.00006, respectively...
  22. ncbi request reprint Immunogenetics of two new HLA alleles: A*0108 and B*4031
    J Street
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, UK
    Tissue Antigens 61:322-4. 2003
    ..Both alleles have frequencies of < 0.0002 in the largely north-western European Caucasoid blood donor population resident in Wales...
  23. ncbi request reprint A modified PCR-SSP method for the identification of ABO blood group antigens
    J Downing
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, UK
    Eur J Immunogenet 30:295-8. 2003
    ..PCR-SSP is an appropriate method for ABO typing of cadaveric organ donors and, importantly, enables both ABO and HLA typing to be performed on the same DNA material...
  24. ncbi request reprint HLA-A, B, C, DRB1, DQB1 matching heterogeneity in 'favourably matched' kidney recipients
    M T Rees
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Ely Valley Road, Talbot Green, Pontyclun CF72 9WB, Wales, UK
    Transpl Immunol 12:73-8. 2003
    ..Little is known about the effects of combinations of mismatched specificities on graft survival. Thus, further investigation is merited particularly for HLA-C and -DQ mismatching...
  25. ncbi request reprint Observations on the HLA-A2403 specificity
    J Street
    Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, UK
    Tissue Antigens 61:330-3. 2003
    ..As it is this 'short' A9/A24 serological reactivity that defines HLA-A2403 all these products will be serologically designated as HLA-A2403...
  26. ncbi request reprint An evaluation of Com-B27, a fluorescein-conjugated mouse anti-HLA-B27 reagent
    E Coates
    nsplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, UK
    Eur J Immunogenet 30:271-4. 2003
    ..However, for consistently reliable B27/B2708 typing we continue to recommend the use of a minimum of two B27 reagents in a protocol that includes DNA-based testing of 'equivocal' B27/B2708 assignments...
  27. ncbi request reprint Identification and nucleotide sequence of two novel DRB3 alleles, DRB3*0102 and DRB3*010133
    M G Guttridge
    Regional Tissue Typing Laboratory, Welsh Blood Service, Cardiff, United Kingdom
    Tissue Antigens 49:665-7. 1997
  28. ncbi request reprint Analysis of the distribution of HLA-B alleles in populations from five continents
    F Williams
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Hum Immunol 62:645-50. 2001
    ..HLA-B allele frequencies and HLA-A/B two locus haplotype frequencies were compiled for each population...
  29. ncbi request reprint The HLA-B7Qui antigen is encoded by a new subtype of HLA-B27 (B*2708)
    W H Hildebrand
    Department of Cell Biology, Stanford University, CA
    Tissue Antigens 44:47-51. 1994