Genomes and Genes
C J Daly
Affiliation: University of Glasgow
- Previously unsuspected widespread cellular and tissue distribution of β-adrenoceptors and its relevance to drug actionC J Daly
School of Life Sciences, College of Medical, Veterinary and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, UK
Trends Pharmacol Sci 32:219-26. 2011....
- Fluorescent ligand binding reveals heterogeneous distribution of adrenoceptors and 'cannabinoid-like' receptors in small arteriesC J Daly
Integrative and Systems Biology, Faculty of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow, UK
Br J Pharmacol 159:787-96. 2010..We have now investigated the distribution of alpha-adrenoceptors, beta-adrenoceptors and cannabinoid-like (GPR55) receptors in the mouse arteries...
- A knockout approach indicates a minor vasoconstrictor role for vascular alpha1B-adrenoceptors in mouseCraig J Daly
Institute of Biomedical and Life Sciences, Division of Neuroscience and Biomedical Systems, University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland
Physiol Genomics 9:85-91. 2002....
- Fluorescent ligands, antibodies, and proteins for the study of receptorsCraig J Daly
Faculty of Biomedical and Life Sciences, Division of Neuroscience and Biomedical Systems, University of Glasgow, Wolfson Building Office 448, West Medical Building Lab 440, University Avenue, G12 8QQ, Glasgow, UK
Pharmacol Ther 100:101-18. 2003....
- Importance of agonists in alpha-adrenoceptor classification and localisation of alpha1-adrenoceptors in human prostateJ C McGrath
Autonomic Physiology Unit, Division of Neurosciences, Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, UK
Eur Urol 36:80-8. 1999..Possible errors in antagonist classification arising from complexity in the actions of agonists and the recently developed method of fluorescent ligand binding on isolated living human prostatic smooth muscle cells will be discussed...
- Quantitative imaging in live human cells reveals intracellular alpha(1)-adrenoceptor ligand-binding sitesJ F Mackenzie
Autonomic Physiology Unit, Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, University of Glasgow, United Kingdom
J Pharmacol Exp Ther 294:434-43. 2000..This provides a potential new site for physiological agonism and makes intracellular access a potential differentiator of drug action...
- Endothelium dependent relaxation in rabbit genital resistance arteries is predominantly mediated by endothelial-derived hyperpolarizing factor in females and nitric oxide in malesJ S Morton
University of Glasgow, Glasgow, UK
J Urol 177:786-91. 2007..We compared nitric oxide and endothelial-derived hyperpolarizing factor contributions to acetylcholine induced relaxation in the genital arteries of the 2 sexes...
- Confocal microscopic characterization of a lesion in a cerebral vessel of the stroke-prone spontaneously hypertensive ratS M Arribas
Clinical Research Initiative in Heart Failure, University of Glasgow, UK
Stroke 27:1118-22; discussion 1122-3. 1996..The effect of perindopril treatment on SHRSP structure and function was also assessed...
- Two "knockout" mouse models demonstrate that aortic vasodilatation is mediated via alpha2a-adrenoceptors located on the endotheliumMajid Malekzadeh Shafaroudi
Autonomic Physiology Unit, Institute of Biomedical and Life Sciences, West Medical Bldg, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
J Pharmacol Exp Ther 314:804-10. 2005..Knockout or genetic malfunction of this receptor should increase arterial stiffness, exacerbated by raised catecholamines, and contribute to heart failure...
- Single-cell recombinant pharmacology: bovine alpha(1a)-adrenoceptors in rat-1 fibroblasts release intracellular ca(2+), display subtype-characteristic agonism and antagonism, and exhibit an antagonist-reversible inverse concentration-response phaseJ D Pediani
Autonomic Physiology Unit, Division of Neuroscience and Biomedical Systems, University of Glasgow, UK
J Pharmacol Exp Ther 293:887-95. 2000..The study also showed a form of fade that could be readily explained...
- Beta-arrestin-dependent spontaneous alpha1a-adrenoceptor endocytosis causes intracellular transportation of alpha-blockers via recycling compartmentsJohn D Pediani
Autonomic Physiology Unit, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
Mol Pharmacol 67:992-1004. 2005..We conclude that alpha(1a)-ARs recycle rapidly by an agonist-independent, constitutive, beta-arrestin-dependent process and that this can transport "alpha-blockers" into cells carrying these receptors...
- New aspects of vascular remodelling: the involvement of all vascular cell typesJohn C McGrath
Autonomic Physiology Unit, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
Exp Physiol 90:469-75. 2005..The objective of this paper is to show how the functions of blood vessels depend on their architecture and a continuous interaction of different cell types and extracellular proteins...
- Do fluorescent drugs show you more than you wanted to know?J C McGrath
Autonomic Physiology Unit, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland
Br J Pharmacol 139:187-9. 2003
- Alpha(1A)-adrenoceptors mediate contractions to phenylephrine in rabbit penile arteriesJ S Morton
Autonomic Physiology Unit, Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow, UK
Br J Pharmacol 150:112-20. 2007..This study seeks the alpha(1)-AR subtypes involved in order to inform the pro-erectile consequences of subtype selective blockade...
- Evidence for involvement of alpha1D-adrenoceptors in contraction of femoral resistance arteries using knockout miceJoseph Zacharia
Vascular Biology Group, Department of Biological and Biomedical Sciences, School of Life Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, Scotland, UK
Br J Pharmacol 146:942-51. 2005..The present study with alpha(1D)-KO mice shows that alpha(1D)-adrenoceptors contribute to vasoconstrictor responses to exogenous and neurally released noradrenaline in femoral resistance arteries...
- Insights into the functional roles of alpha(1)-adrenoceptor subtypes in mouse carotid arteries using knockout miceClare Deighan
Autonomic Physiology Unit, Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow
Br J Pharmacol 144:558-65. 2005....
- 3-d image analysis of fluorescent drug bindingM Raquel Miquel
Universidad de Valencia, Valencia, Spain
Mol Imaging 4:40-52. 2005..Although our application is specific to adrenergic receptors, the general method could be applied to any volumetric, fluorescence-image-based assay...
- Role of elastin in spontaneously hypertensive rat small mesenteric artery remodellingAna M Briones
Departamento de Fisiologia, Facultad de Medicina, Universidad Autonoma de Madrid, C Arzobispo Morcillo 2, 28029 Madrid, Spain
J Physiol 552:185-95. 2003..Differences in elastin organisation are, therefore, a central element in small artery remodelling in hypertension...
- Confocal myography for the study of hypertensive vascular remodellingSilvia M Arribas
Universidad Autonoma de Madrid, Spain
Clin Hemorheol Microcirc 37:205-10. 2007..Confocal myography offers a new approach to the study of vascular remodelling in intact small arteries from a 3D point of view...
- Direct demonstration of beta1- and evidence against beta2- and beta3-adrenoceptors, in smooth muscle cells of rat small mesenteric arteriesAna M Briones
Departament de Farmacologia, de Terapeutica i de Toxicologia, , Facultat de Medicina, , Bellatera, Spain
Br J Pharmacol 146:679-91. 2005..Furthermore, we have demonstrated, for the first time, the usefulness of BODIPY TMR-CGP 12177 for identifying beta-AR distribution in the 'living' vascular wall...
- Postnatal alterations in elastic fiber organization precede resistance artery narrowing in SHRJose M Gonzalez
Universidad Autonoma de Madrid, C Arzobispo Morcillo 2, 28029 Madrid, Spain
Am J Physiol Heart Circ Physiol 291:H804-12. 2006..These data suggest that abnormal elastic fiber deposition in SHR increases resistance artery stiffness at an early age, which might participate in vessel narrowing later in life...
- The role of the alpha1B-adrenergic receptor in vascular structure and functionCraig J Daly
Johns Hopkins U Sch Med, Baltimore, MD
Hypertension 45:e20; author reply e20-1. 2005
- Imaging the vascular wall using confocal microscopySilvia M Arribas
Departamento de Fisiologia, Facultad de Medicina, Universidad Autonoma de Madrid, Madrid, Spain
J Physiol 584:5-9. 2007..In conclusion, confocal microscopy and image analysis software provide insight into vascular wall structure and function and the active process of vascular remodelling in physiological and pathological situations...
- Visualisation of morphological changes in living intact human microvessels using confocal microscopyShabaz A Hamid
Royal Veterinary College, London NW1 0TU, UK
Microvasc Res 69:173-7. 2005....