Research Topics
Genomes and Genes | Jacob Z. DalgaardSummaryAffiliation: University of Warwick Location: Coventry, UK URL: http://www2.warwick.ac.uk/fac/med/research/csri/dnalab Publications
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Publications
Protein-coding introns from the 23S rRNA-encoding gene form stable circles in the hyperthermophilic archaeon Pyrobaculum organotrophumJ Z Dalgaard
Institute of Biological Chemistry B, Copenhagen University, Denmark
Gene 121:103-10. 1992..Putative proteins encoded by the introns and covering most of the intron sequence share a decapeptide motif with proteins encoded by another archaeal intron and by group I introns...
Complex mechanism of site-specific DNA replication termination in fission yeastSandra Codlin
Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
EMBO J 22:3431-40. 2003..Interestingly, RTS1 displays similarities to mammalian rDNA replication barriers...- RNase-sensitive DNA modification(s) initiates S. pombe mating-type switchingSonya Vengrova
Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
Genes Dev 18:794-804. 2004..We propose a model in which a swi1- and swi3-dependent signal during lagging-strand synthesis leads to pausing of leading-strand replication and the introduction of the imprint... - Selective gene expression in multigene families from yeast to mammalsJacob Z Dalgaard
Marie Curie Research Institute, The Chart, Oxted RH8 0TL, Surrey, UK
Sci STKE 2004:re17. 2004.... - Schizosaccharomyces pombe Swi1, Swi3, and Hsk1 are components of a novel S-phase response pathway to alkylation damageElena Sommariva
Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom
Mol Cell Biol 25:2770-84. 2005..In conclusion, Swi1, Swi3, and Hsk1 act in a novel S-phase checkpoint pathway that contributes to replication fork maintenance and to survival of alkylation damage... - SMC5 and SMC6 genes are required for the segregation of repetitive chromosome regionsJordi Torres-Rosell
Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
Nat Cell Biol 7:412-9. 2005..These results point towards a role for the Smc5-Smc6 complex in preventing the formation of sister chromatid junctions, thereby ensuring the correct partitioning of chromosomes during anaphase... - The Schizosaccharomyces pombe imprint--nick or ribonucleotide(s)?S Vengrova
Curr Biol 15:R326-7; author reply R327. 2005 - The wild-type Schizosaccharomyces pombe mat1 imprint consists of two ribonucleotidesSonya Vengrova
Marie Curie Research Institute, The Chart, Oxted, Surrey, UK
EMBO Rep 7:59-65. 2006..Second, using a novel assay based on ligation-mediated PCR, a 3'-terminal ribonucleotide is detected at the hydrolysed imprint. Our observations allow the unification of available data sets characterizing the wild-type imprint... - Schizosaccharomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanismTomoko Yamada Inagawa
Marie Curie Research Institute, Surry RH8 0TL, United Kingdom
Genetics 177:255-65. 2007..We show that in such strains wild-type P-cassette DNA is efficiently generated at mat1 through heteroduplex DNA formation and repair. The present data provide an in vivo genetic test of the proposed molecular recombination mechanism... - Rtf1-mediated eukaryotic site-specific replication terminationT Eydmann
Marie Curie Research Institute, The Chart, Oxted RH8 0TL, United Kingdom
Genetics 180:27-39. 2008..Together our data establish that multiple protein DNA and protein-protein interactions between Rtf1 molecules and both the repeated motifs and the enhancer region of RTS1 are required for site-specific termination at the RTS1 element... - Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restartTakabumi Inagawa
Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom
Proc Natl Acad Sci U S A 106:7927-32. 2009..Our results are consistent with a model in which Rtf2 stabilizes the replication fork stalled at RTS1 until completion of DNA synthesis by a converging replication fork initiated at a flanking origin... 
Random and site-specific replication terminationJacob Z Dalgaard
Marie Curie Research Institute, The Chart, Oxted, Surrey, UK
Methods Mol Biol 521:35-53. 2009..This review gives an overview about what is known about replication termination, with a focus on these natural site-specific replication termination sites...- High-resolution mapping of points of site-specific replication stallingSonya Vengrova
Marie Curie Research Institute, The Chart, Oxted, Surrey, UK
Methods Mol Biol 521:215-27. 2009..The data obtained using this method allow determining the position of the 3'-end of the nascent strand at a stalled fork with a one-nucleotide resolution... - The many facets of the Tim-Tipin protein families' roles in chromosome biologyRamsay J McFarlane
North West Cancer Research Fund Institute, College of Natural Sciences, Bangor University, Bangor, Gwynedd, UK
Cell Cycle 9:700-5. 2010..Here we report on our current understanding of the many facets of these protein families in maintaining genome stability and replication checkpoint control... - Identification of a novel type of spacer element required for imprinting in fission yeastSuha Sayrac
Clinical Sciences Research Institute, Gibbet Hill Campus, University of Warwick, Coventry, United Kingdom
PLoS Genet 7:e1001328. 2011..The presented work addresses how differentiated sister chromatids are established during DNA replication through the involvement of replication barriers... - Comparative functional genomics of the fission yeastsNicholas Rhind
Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
Science 332:930-6. 2011..These analyses elucidate the genome structure and gene regulation of fission yeast and provide tools for investigation across the Schizosaccharomyces clade... - A mark in the core: silence no more!Patrick D Varga-Weisz
Marie Curie Research Institute, The Chart, Oxted RH8 0TL, Surrey, UK
Mol Cell 9:1154-6. 2002..Dot1p is a new type of methyltransferase that methylates lysine 79 in the histone H3 core only in its nucleosomal context and has a possible role in marking open chromatin regions... - RTS1-an eukaryotic terminator of replicationSonya Vengrova
Marie Curie Research Institute, The Chart, Oxted, Surrey RH80TL, UK
Int J Biochem Cell Biol 34:1031-4. 2002..This is the first replication terminator shown to play a role in cellular differentiation... - A DNA replication-arrest site RTS1 regulates imprinting by determining the direction of replication at mat1 in S. pombeJ Z Dalgaard
Marie Curie Research Institute MCRI, The Chart, Oxted, Surrey RH8 0TL, UK
Genes Dev 15:2060-8. 2001..Such elements could play a more general role during development and differentiation in higher eukaryotes by regulating the direction of DNA replication at key loci... - Prokaryotic introns and inteins: a panoply of form and functionM Belfort
Molecular Genetics Program, Wadsworth Center and School of Public Health, State University of New York at Albany 12201 2002, USA
J Bacteriol 177:3897-903. 1995 - Purification and characterization of two forms of I-DmoI, a thermophilic site-specific endonuclease encoded by an archaeal intronJ Z Dalgaard
Molecular Genetics Program, Wadsworth Center, New York State Department of Health, Albany 12201 0509
J Biol Chem 269:28885-92. 1994..These results support the view that these phylogenetically diverse enzymes, which function to mobilize the DNA sequences that encode them, share a common ancestry... - Mobile introns and inteins: friend or foe?J Z Dalgaard
Trends Genet 10:306-7. 1994 - A site-specific endonuclease encoded by a typical archaeal intronJ Z Dalgaard
Molecular Genetics Program, Wadsworth Center for Laboratories, New York State Department of Health, Albany 12201 0509
Proc Natl Acad Sci U S A 90:5414-7. 1993..These observations support the independent evolutionary origin of endonucleases and intron core elements and are consistent with the invasive potential of endonuclease genes... - Intercellular mobility and homing of an archaeal rDNA intron confers a selective advantage over intron- cells of Sulfolobus acidocaldariusC Aagaard
Institute of Molecular Biology, Copenhagen University, Denmark
Proc Natl Acad Sci U S A 92:12285-9. 1995..By using a double drug-resistant mutant of S. acidocaldarius, it was shown that spreading resulted partly from a selective advantage of intron+ cells and partly from intercellular mobility of the intron and homing... - A novel Ty1-mediated fragmentation method for native and artificial yeast chromosomes reveals that the mouse steel gene is a hotspot for Ty1 integrationJ Z Dalgaard
Molecular Genetics Program, Wadsworth Center, David Axelrod Institute, Albany, New York, USA
Genetics 143:673-83. 1996..The accessibility of mammalian transcription units to Ty1 insertion stands in contrast to that of yeast transcription units... - Statistical modeling, phylogenetic analysis and structure prediction of a protein splicing domain common to inteins and hedgehog proteinsJ Z Dalgaard
NCI Frederick Cancer Research and Development Center, ABL Basic Research Program, Frederick, Maryland 21702 1201, USA
J Comput Biol 4:193-214. 1997..This domain may play a role in formation of surface-associated protein complexes... - Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolutionV Derbyshire
New York State Department of Health, School of Public Health, State University of New York at Albany, Albany, NY 12201 2002, USA
Proc Natl Acad Sci U S A 94:11466-71. 1997.... - Statistical modeling and analysis of the LAGLIDADG family of site-specific endonucleases and identification of an intein that encodes a site-specific endonuclease of the HNH familyJ Z Dalgaard
NCI Frederick Cancer Research and Development Center, ABL Basic Research Program, PO Box B, Building 549, Room 154, Frederick, MD 21702 1202, USA
Nucleic Acids Res 25:4626-38. 1997..However, there does appear to have been considerable exchange of endonuclease domains amongst elements of the same type. Such events are suggested to be important for the formation of elements of new specficity... - Multiple epigenetic events regulate mating-type switching of fission yeastA J Klar
National Cancer Institute Frederick Cancer Research, Frederick, MD 21702 1201, USA
Novartis Found Symp 214:87-99; discussion 99-103. 1998..We speculate that the unit of inheritance in the mat2/3 interval is both DNA plus its associated chromatin structure. Such a control is likely to be essential in maintaining particular states of gene expression during development... - Crystal structure of the thermostable archaeal intron-encoded endonuclease I-DmoIG H Silva
Wadsworth Center, New York State Department of Health, Albany, NY, 12201 0509, USA
J Mol Biol 286:1123-36. 1999..This is reversed compared to PI-SceI, where the amino-terminal domain is more similar to carboxy-terminal domain of I-DmoI and to I-CreI, with interesting evolutionary implications... - Crystallization and preliminary crystallographic analysis of the archaeal intron-encoded endonuclease I-DmoIJ Z Dalgaard
Wadsworth Center, New York State Department of Health, PO Box 509, Albany, NY 12201, USA
Acta Crystallogr D Biol Crystallogr 54:1435-6. 1998..03, c = 55.56 A, beta = 113.4 degrees, with one molecule per asymmetric unit (Vm = 2.01 A3 Da-1). The crystals diffract to at least 2.3 A resolution. A complete native data set has been measured and structure determination is on-going... - Orientation of DNA replication establishes mating-type switching pattern in S. pombeJ Z Dalgaard
Gene Regulation and Chromosome Biology Laboratory, ABL Basic Research Program, NCI Frederick Cancer Research and Development Center, Maryland 21702 1201, USA
Nature 400:181-4. 1999..Two-dimensional gel analysis confirmed that mat1 is preferentially replicated by a centromere-distal origin(s). Thus, the DNA replication machinery may confer different developmental potential to sister cells... - swi1 and swi3 perform imprinting, pausing, and termination of DNA replication in S. pombeJ Z Dalgaard
DHHS, NCI, Division of Basic Sciences Gene Regulation and Chromosome Biology Laboratory, NCI Frederick Cancer Research and Development Center, Maryland 21702, USA
Cell 102:745-51. 2000..These results suggest that swi1p and swi3p promote imprinting in novel ways both by pausing replication at mat1 and by terminating replication at RTS1... - Does S. pombe exploit the intrinsic asymmetry of DNA synthesis to imprint daughter cells for mating-type switching?J Z Dalgaard
Marie Curie Research Institute, The Chart, Oxted, Surrey, UK RH8 0TL
Trends Genet 17:153-7. 2001..The discovery raises the possibility that DNA replication might be more generally involved in the establishment of asymmetric cellular differentiation... - Causes and consequences of ribonucleotide incorporation into nuclear DNAJacob Z Dalgaard
Division of Biomedical Cell Biology, Gibbet Hill Campus, University of Warwick, Coventry, CV4 7AL, UK Electronic address
Trends Genet 28:592-7. 2012..Indeed, genomic ribonucleotides might have diverse roles affecting genetic stability, DNA damage repair, heterochromatin formation, cellular differentiation, and development...