Matthew John Dalby

Summary

Affiliation: University of Glasgow
Country: UK

Publications

  1. ncbi request reprint Investigating the limits of filopodial sensing: a brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia
    M J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Cell Biol Int 28:229-36. 2004
  2. ncbi request reprint Fibroblast signaling events in response to nanotopography: a gene array study
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, G12 8QQ Glasgow, UK
    IEEE Trans Nanobioscience 1:12-7. 2002
  3. ncbi request reprint Morphological and microarray analysis of human fibroblasts cultured on nanocolumns produced by colloidal lithography
    M J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Eur Cell Mater 9:1-8; discussion 8. 2005
  4. ncbi request reprint Investigating filopodia sensing using arrays of defined nano-pits down to 35 nm diameter in size
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Int J Biochem Cell Biol 36:2005-15. 2004
  5. ncbi request reprint Use of nanotopography to study mechanotransduction in fibroblasts--methods and perspectives
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Eur J Cell Biol 83:159-69. 2004
  6. ncbi request reprint Nanotopographical control of human osteoprogenitor differentiation
    Matthew J Dalby
    Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Curr Stem Cell Res Ther 2:129-38. 2007
  7. pmc Cellular response to low adhesion nanotopographies
    Matthew J Dalby
    Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, UK
    Int J Nanomedicine 2:373-81. 2007
  8. ncbi request reprint Topographically induced direct cell mechanotransduction
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    Med Eng Phys 27:730-42. 2005
  9. ncbi request reprint Osteoprogenitor response to defined topographies with nanoscale depths
    Matthew John Dalby
    Division of Infection and Immunity, Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Biomaterials 27:1306-15. 2006
  10. ncbi request reprint Osteoprogenitor response to semi-ordered and random nanotopographies
    Matthew John Dalby
    Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Biomaterials 27:2980-7. 2006

Detail Information

Publications38

  1. ncbi request reprint Investigating the limits of filopodial sensing: a brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia
    M J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Cell Biol Int 28:229-36. 2004
    ..Fluorescence microscopy has also been used to look at adhesion formation. The results showed distinct filopodial/nano-island interaction and changes in adhesion morphology...
  2. ncbi request reprint Fibroblast signaling events in response to nanotopography: a gene array study
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, G12 8QQ Glasgow, UK
    IEEE Trans Nanobioscience 1:12-7. 2002
    ..The use of microarray has expanded the range of observations possible using established microscopical and biochemical techniques...
  3. ncbi request reprint Morphological and microarray analysis of human fibroblasts cultured on nanocolumns produced by colloidal lithography
    M J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Eur Cell Mater 9:1-8; discussion 8. 2005
    ..The results indicate that changes in cell spreading correlate with a number of gene up- and down-regulations as will be described within the manuscript...
  4. ncbi request reprint Investigating filopodia sensing using arrays of defined nano-pits down to 35 nm diameter in size
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Int J Biochem Cell Biol 36:2005-15. 2004
    ..We suggest that this may occur through nanocontact guidance as filopodia are moved over the pits...
  5. ncbi request reprint Use of nanotopography to study mechanotransduction in fibroblasts--methods and perspectives
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Eur J Cell Biol 83:159-69. 2004
    ....
  6. ncbi request reprint Nanotopographical control of human osteoprogenitor differentiation
    Matthew J Dalby
    Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Curr Stem Cell Res Ther 2:129-38. 2007
    ..Finally, it will make some speculations of the future of nanomaterials and progenitor cells in tissue engineering...
  7. pmc Cellular response to low adhesion nanotopographies
    Matthew J Dalby
    Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, UK
    Int J Nanomedicine 2:373-81. 2007
    ..Finally, it is hypothesized as to how specifically low-adhesion materials may fit into the outlined mechanotransductive mechanisms...
  8. ncbi request reprint Topographically induced direct cell mechanotransduction
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    Med Eng Phys 27:730-42. 2005
    ..Thus, this review also describes cell responses to topography...
  9. ncbi request reprint Osteoprogenitor response to defined topographies with nanoscale depths
    Matthew John Dalby
    Division of Infection and Immunity, Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Biomaterials 27:1306-15. 2006
    ..It is concluded that the human bone marrow stromal cells are highly responsive to nanoscale features...
  10. ncbi request reprint Osteoprogenitor response to semi-ordered and random nanotopographies
    Matthew John Dalby
    Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Biomaterials 27:2980-7. 2006
    ..The results demonstrated that the nanotopographies stimulated the osteoprogenitor cell differentiation towards an osteoblastic phenotype...
  11. ncbi request reprint Interactions of human blood and tissue cell types with 95-nm-high nanotopography
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, G12 8QQ Glasgow, UK
    IEEE Trans Nanobioscience 1:18-23. 2002
    ..This is the normal, in vivo, morphology for vascular endothelium. This result suggests that the nano-features are promoting a more phenotypically correct morphology...
  12. ncbi request reprint Nanotopographical stimulation of mechanotransduction and changes in interphase centromere positioning
    Matthew J Dalby
    Division of Infection and Immunity, Centre for Cell Engineering, Joseph Black Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Cell Biochem 100:326-38. 2007
    ..We have also obtained evidence that these subtrata result in downregulation of gene expression which suggests that this may be due to the changed tension on the nucleus: an intriguing possibility that merits further investigation...
  13. ncbi request reprint The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder
    Matthew J Dalby
    Centre for Cell Engineering, Joseph Black Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, UK
    Nat Mater 6:997-1003. 2007
    ..In addition, the current studies show that topographically treated MSCs have a distinct differentiation profile compared with those treated with osteogenic media, which has implications for cell therapies...
  14. ncbi request reprint The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, Scotland, United Kingdom
    J Biomed Mater Res A 84:973-9. 2008
    ..The results show reduction in cellular adhesion, decrease in spreading, and a broad genomic down-regulation. Also noted was an increase in endocytotic activity in cells on the pits...
  15. ncbi request reprint Group analysis of regulation of fibroblast genome on low-adhesion nanostructures
    Matthew J Dalby
    Centre for Cell Engineering, Joseph Black Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Biomaterials 28:1761-9. 2007
    ..We also feel that the analysis used could be of interest to biomaterials scientists looking for easy ways to display microarray data efficiently...
  16. ncbi request reprint Changes in fibroblast morphology in response to nano-columns produced by colloidal lithography
    Matthew John Dalby
    Centre for Cell Engineering Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Biomaterials 25:5415-22. 2004
    ..By understanding as to which features evoke spatial reactions in cells, it may be possible to design better biomaterials...
  17. ncbi request reprint Attempted endocytosis of nano-environment produced by colloidal lithography by human fibroblasts
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    Exp Cell Res 295:387-94. 2004
    ..These observations could have implications for nanomaterials science in areas such as cell transfection and drug delivery...
  18. ncbi request reprint Increasing hydroxyapatite incorporation into poly(methylmethacrylate) cement increases osteoblast adhesion and response
    M J Dalby
    IRC in Biomedical Materials, Institute of Orthopaedics, Stanmore, Middlesex, UK
    Biomaterials 23:569-76. 2002
    ..Preferential anchorage of HOBs to HA rather than PMMA was a prominent observation...
  19. pmc Genomic expression of mesenchymal stem cells to altered nanoscale topographies
    Matthew J Dalby
    Centre for Cell Engineering, Joseph Black Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    J R Soc Interface 5:1055-65. 2008
    ..The results further demonstrate that perhaps the shape of the extracellular matrix is critical to tissue development...
  20. ncbi request reprint Initial attachment of osteoblasts to an optimised HAPEX topography
    M J Dalby
    IRC in Biomedical Materials, Institute of Orthopaedics, Stanmore, Middlesex, UK
    Biomaterials 23:681-90. 2002
    ..The results show that the cells attach in greater numbers to the optimised surface, and develop notably faster, than cells on machined HAPEX...
  21. ncbi request reprint Increasing fibroblast response to materials using nanotopography: morphological and genetic measurements of cell response to 13-nm-high polymer demixed islands
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, United Kingdom
    Exp Cell Res 276:1-9. 2002
    ..Microscopical results provide confirmation of the microarray findings...
  22. ncbi request reprint In vitro reaction of endothelial cells to polymer demixed nanotopography
    M J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, UK
    Biomaterials 23:2945-54. 2002
    ..Of the nanotopographies, the 13 nm islands were seen to give the largest response, with highly spread cell morphologies containing well-defined cytoskeleton...
  23. ncbi request reprint Optimizing HAPEX topography influences osteoblast response
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 800, UK
    Tissue Eng 8:453-67. 2002
    ..Transmission electron microscopy has also been used to look at more long-term morphology. The results show that topography significantly influences cell response, and may be a means of enhancing bone apposition on HAPEX...
  24. ncbi request reprint Fibroblast reaction to island topography: changes in cytoskeleton and morphology with time
    M J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow, Scotland G12 8QQ, UK
    Biomaterials 24:927-35. 2003
    ..The results also show a change in cell response from initial fast organisation of cytoskeleton in reaction to the islands, through to lack of cell spreading and low recruitment of cell numbers on the islands...
  25. ncbi request reprint Polymer-demixed nanotopography: control of fibroblast spreading and proliferation
    M J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Tissue Eng 8:1099-108. 2002
    ..Interaction of filopodia with the islands could been seen to increase as island size was increased...
  26. ncbi request reprint Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography
    Matthew J Dalby
    Centre for Cell Engineering, Faculty of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Exp Cell Res 284:274-82. 2003
    ..5 x 2-microm grooves) with time. The results showed many initial gene changes and also down-regulation of gene response with time. Cell and nucleus morphology were also considered, with nuclear deformation linked to cell signaling...
  27. ncbi request reprint Rapid fibroblast adhesion to 27nm high polymer demixed nano-topography
    M J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow, G12 8QQ, UK
    Biomaterials 25:77-83. 2004
    ..The results showed that an initial rapid adhesion and cytoskeletal formation on the islands at 4 days of culture gave way to poorly formed contacts and vimentin cytoskeleton at 30 days of culture...
  28. ncbi request reprint Nonadhesive nanotopography: fibroblast response to poly(n-butyl methacrylate)-poly(styrene) demixed surface features
    M J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, United Kingdom
    J Biomed Mater Res A 67:1025-32. 2003
    ..The results indicated that the cells do not respond differently to the 10-nm islands compared with planar samples but, in contrast, the 50-nm islands are nonadhesive...
  29. ncbi request reprint Fibroblast response to a controlled nanoenvironment produced by colloidal lithography
    Matthew J Dalby
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    J Biomed Mater Res A 69:314-22. 2004
    ..By studying cell adhesion with time and cytoskeletal (actin, tubulin, and vimentin) maturity, insight has been gained as to how fibroblasts adhere to these nanofeatures...
  30. ncbi request reprint Nanomechanotransduction and interphase nuclear organization influence on genomic control
    Matthew J Dalby
    Centre for Cell Engineering, Joseph Black Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, Scotland, UK
    J Cell Biochem 102:1234-44. 2007
    ..We hypothesize that as changes in cell and thus nuclear morphology occur, that this may alter the probability of transcription through opening or closing areas of the chromosomes to transcription factors...
  31. ncbi request reprint Initial interaction of osteoblasts with the surface of a hydroxyapatite-poly(methylmethacrylate) cement
    M J Dalby
    IRC in Biomedical Materials, Institute of Orthopaedics, Stanmore, Middlesex, UK
    Biomaterials 22:1739-47. 2001
    ..A significantly higher cell proliferation and differentiation was seen on the PMMA/HA cement compared to the PMMA cement alone, with retention of phenotype up to 21 days of culture on both materials...
  32. ncbi request reprint The effects of nanoscale pits on primary human osteoblast adhesion formation and cellular spreading
    M J P Biggs
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK
    J Mater Sci Mater Med 18:399-404. 2007
    ..This study is specifically concerned with the effects these nanotopographies have on adhesion formation in S-phase osteoblasts as identified by BrdU incorporation. Nanopits were found to reduce cellular spreading and adhesion formation...
  33. ncbi request reprint Regulation of implant surface cell adhesion: characterization and quantification of S-phase primary osteoblast adhesions on biomimetic nanoscale substrates
    Manus J P Biggs
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, United Kingdom
    J Orthop Res 25:273-82. 2007
    ..It is postulated that nanopits affect osteoblast-substrate adhesion by directly or indirectly affecting adhesion complex formation, a phenomenon dependent on nanopit dimension and conformation...
  34. ncbi request reprint The interaction of human bone marrow cells with nanotopographical features in three dimensional constructs
    C C Berry
    Department of Electronics and Electrical Engineering, Centre for Cell Engineering, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    J Biomed Mater Res A 79:431-9. 2006
    ..The results show that nanofeatures of size X, diameter Y, and spacing Z decrease cell spreading, reduce cytoskeletal organization, and increase endocytotic activity within the cells...
  35. ncbi request reprint Cells react to nanoscale order and symmetry in their surroundings
    A S G Curtis
    Centre for Cell Engineering, University of Glasgow, Glasgow G12 8QQ, UK
    IEEE Trans Nanobioscience 3:61-5. 2004
    ..The cells appear to be able to distinguish between different symmetries of array. We suggest that interfacial forces may be organized by the nanostructures to affect the cells in the same way as they affect liquid crystal orientations...
  36. pmc Adhesion formation of primary human osteoblasts and the functional response of mesenchymal stem cells to 330nm deep microgrooves
    M J P Biggs
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    J R Soc Interface 5:1231-42. 2008
    ..This study indicates that groove/ridge topographies are important modulators of both cellular adhesion and osteospecific function and, critically, that groove/ridge width is important in determining cellular response...
  37. doi request reprint Focal adhesion interactions with topographical structures: a novel method for immuno-SEM labelling of focal adhesions in S-phase cells
    M J P Biggs
    Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    J Microsc 231:28-37. 2008
    ..Osteoblast adhesion to these nanotopographies was ascertained by quantification of adhesion complex formation...
  38. ncbi request reprint Osteoblast behaviour on HA/PE composite surfaces with different HA volumes
    L Di Silvio
    Institute of Orthopaedics, IRC in Biomedical Materials, Stanmore, Middlesex, UK
    Biomaterials 23:101-7. 2002
    ..The cytoskeletal organisation of the cells was also examined and HOBs cultured on 40% HA/PE were flatter and had an enhanced rate of cytoskeletal organisation and an increase in focal contact points compared to the 20% HA/PE...