P J Cullen

Summary

Affiliation: University of Bristol
Country: UK

Publications

  1. doi request reprint Phosphoinositides in the Mammalian Endo-lysosomal Network
    Peter J Cullen
    Henry Wellcome Integrated Signaling Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, BS8 1TD, Bristol, United Kingdom
    Subcell Biochem 59:65-110. 2012
  2. ncbi request reprint Integration of calcium and Ras signalling
    Peter J Cullen
    The Henry Wellcome Laboratories for Integrated Cell Signalling, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Nat Rev Mol Cell Biol 3:339-48. 2002
  3. ncbi request reprint Modular phosphoinositide-binding domains--their role in signalling and membrane trafficking
    P J Cullen
    Inositide Group, Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Biol 11:R882-93. 2001
  4. ncbi request reprint Ras effectors: buying shares in Ras plc
    P J Cullen
    Department of Biochemistry, University of Bristol, BS8 1TD, Bristol, UK
    Curr Biol 11:R342-4. 2001
  5. ncbi request reprint Decoding complex Ca2+ signals through the modulation of Ras signaling
    Peter J Cullen
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Opin Cell Biol 18:157-61. 2006
  6. ncbi request reprint GAP1IP4BP contains a novel group I pleckstrin homology domain that directs constitutive plasma membrane association
    G E Cozier
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 275:28261-8. 2000
  7. ncbi request reprint Molecular cloning and functional characterization of a human homologue of centaurin-alpha
    K Venkateswarlu
    School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, United Kingdom
    Biochem Biophys Res Commun 262:237-44. 1999
  8. pmc Identification of centaurin-alpha1 as a potential in vivo phosphatidylinositol 3,4,5-trisphosphate-binding protein that is functionally homologous to the yeast ADP-ribosylation factor (ARF) GTPase-activating protein, Gcs1
    K Venkateswarlu
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, U K
    Biochem J 340:359-63. 1999
  9. ncbi request reprint EGF-and NGF-stimulated translocation of cytohesin-1 to the plasma membrane of PC12 cells requires PI 3-kinase activation and a functional cytohesin-1 PH domain
    K Venkateswarlu
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 112:1957-65. 1999
  10. ncbi request reprint Identification of the ras GTPase-activating protein GAP1(m) as a phosphatidylinositol-3,4,5-trisphosphate-binding protein in vivo
    P J Lockyer
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Biol 9:265-8. 1999

Collaborators

  • P J Lockyer
  • J M Tavare
  • G Banting
  • T J McNulty
  • J Downward
  • S Vanlingen
  • T R Jackson
  • Zhong Ying Shen
  • G A Rutter
  • David J Stephens
  • P Chardin
  • M R Philips
  • M D Bootman
  • H Mellor
  • H L Roderick
  • J Kremerskothen
  • Sabine Kupzig
  • Simon A Walker
  • K Venkateswarlu
  • Dalila Bouyoucef
  • Gyles E Cozier
  • G E Cozier
  • Qing Liu
  • Trever G Bivona
  • Hongchuan Jin
  • Colin J Traer
  • Giles O C Cory
  • S Yarwood
  • T H Millard
  • James A Taylor
  • Jez Carlton
  • S A Walker
  • S Kupzig
  • P B Oatey
  • Jez G Carlton
  • Jianming Ying
  • Anthony T C Chan
  • En Min Li
  • Gopesh Srivastava
  • Naomi Attar
  • Anna C Rutherford
  • F Gunn-Moore
  • Xian Wang
  • Ada H Y Wong
  • Jacqueline Oakley
  • Thomas Wassmer
  • Jie Jin
  • Yan Cui
  • Qian Tao
  • Qian Zhang
  • Krysten J Palmer
  • Delia Deaconescu
  • Oliver Daumke
  • Alfred Wittinghofer
  • Christian L Polte
  • D Bouyoucef-Cherchalli
  • Toshimasa Ishizaki
  • Yan Feng Dai
  • Dingcheng Gao
  • Rebecca S Arkell
  • Maxine J Allen
  • Alan Buckler
  • Louise C Davies
  • Judith Klumperman
  • Miriam Bujny
  • J Carlton
  • Brian J Peter
  • Takashi Tsuboi
  • D Bouyoucef
  • Anna Rutherford
  • Harvey T McMahon
  • Viola M J Oorschot
  • Angel Pellicer
  • Vi K Chiu
  • Ignacio Perez de Castro
  • Ian M Ahearn
  • Theresa M Grana
  • Adrienne D Cox
  • Sara Bilu
  • Dorit Zharhary
  • J R Bottomley
  • J S Reynolds

Detail Information

Publications35

  1. doi request reprint Phosphoinositides in the Mammalian Endo-lysosomal Network
    Peter J Cullen
    Henry Wellcome Integrated Signaling Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, BS8 1TD, Bristol, United Kingdom
    Subcell Biochem 59:65-110. 2012
    ..Herein we discuss regulation of these machineries by phosphoinositides and explore how phosphoinositide-switching contributes toward sorting decisions made at this platform...
  2. ncbi request reprint Integration of calcium and Ras signalling
    Peter J Cullen
    The Henry Wellcome Laboratories for Integrated Cell Signalling, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Nat Rev Mol Cell Biol 3:339-48. 2002
    ..Here, we focus on examining the link between calcium and Ras signalling and, in particular, we speculate as to how the complexity of calcium signalling could regulate Ras activity...
  3. ncbi request reprint Modular phosphoinositide-binding domains--their role in signalling and membrane trafficking
    P J Cullen
    Inositide Group, Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Biol 11:R882-93. 2001
    ..Here, with particular reference to proteins involved in membrane traffic pathways, we discuss recent advances in our understanding of phosphoinositide-binding domains...
  4. ncbi request reprint Ras effectors: buying shares in Ras plc
    P J Cullen
    Department of Biochemistry, University of Bristol, BS8 1TD, Bristol, UK
    Curr Biol 11:R342-4. 2001
    ..The argument may be resolved by the recent identification of a novel Ras-regulated PLC, but some unexpected properties of this protein are sure to stimulate further controversy...
  5. ncbi request reprint Decoding complex Ca2+ signals through the modulation of Ras signaling
    Peter J Cullen
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Opin Cell Biol 18:157-61. 2006
    ..Here I describe some recent advances that have shed light on how cells can decode the spatial and temporal aspects of Ca(2+) signals through the regulation of this important signalling switch...
  6. ncbi request reprint GAP1IP4BP contains a novel group I pleckstrin homology domain that directs constitutive plasma membrane association
    G E Cozier
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 275:28261-8. 2000
    ....
  7. ncbi request reprint Molecular cloning and functional characterization of a human homologue of centaurin-alpha
    K Venkateswarlu
    School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, United Kingdom
    Biochem Biophys Res Commun 262:237-44. 1999
    ..These results suggest that centaurin-alpha(1) can function as an in vivo PtdIns(3, 4,5)P(3) receptor...
  8. pmc Identification of centaurin-alpha1 as a potential in vivo phosphatidylinositol 3,4,5-trisphosphate-binding protein that is functionally homologous to the yeast ADP-ribosylation factor (ARF) GTPase-activating protein, Gcs1
    K Venkateswarlu
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, U K
    Biochem J 340:359-63. 1999
    ....
  9. ncbi request reprint EGF-and NGF-stimulated translocation of cytohesin-1 to the plasma membrane of PC12 cells requires PI 3-kinase activation and a functional cytohesin-1 PH domain
    K Venkateswarlu
    Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 112:1957-65. 1999
    ..This data therefore suggests that in vivo cytohesin-1 can interact with PIP3 via its PH domain...
  10. ncbi request reprint Identification of the ras GTPase-activating protein GAP1(m) as a phosphatidylinositol-3,4,5-trisphosphate-binding protein in vivo
    P J Lockyer
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Biol 9:265-8. 1999
    ..From these results, we conclude that GAP1(m) binds PIP3 in vivo, and it is recruited to the plasma membrane, but does not appear to be activated, following agonist stimulation of PI 3-kinase...
  11. ncbi request reprint Tissue-specific expression and endogenous subcellular distribution of the inositol 1,3,4,5-tetrakisphosphate-binding proteins GAP1(IP4BP) and GAP1(m)
    P J Lockyer
    Department of Biochemistry, School of Medical Sciences, University of Bristol, United Kingdom
    Biochem Biophys Res Commun 255:421-6. 1999
    ..As GAP1(m) is primarily localised to the cytosol of unstimulated cells it may be spatially regulated in order to interact with Ras at the plasma membrane...
  12. pmc Nerve growth factor- and epidermal growth factor-stimulated translocation of the ADP-ribosylation factor-exchange factor GRP1 to the plasma membrane of PC12 cells requires activation of phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology domain
    K Venkateswarlu
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochem J 335:139-46. 1998
    ..Taken together these data strongly suggest that GRP1 interacts in vivo with plasma membrane-located PtdIns(3,4,5)P3 and hence constitutes a true PtdIns(3,4,5)P3 receptor...
  13. ncbi request reprint Membrane targeting by pleckstrin homology domains
    G E Cozier
    Inositide Group, Henry Wellcome Integrated Signaling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Top Microbiol Immunol 282:49-88. 2004
    ....
  14. ncbi request reprint CAPRI regulates Ca(2+)-dependent inactivation of the Ras-MAPK pathway
    P J Lockyer
    Department of Biochemistry, School of Medical Sciences, University of Bristol, BS8 1TD, Bristol, United Kingdom
    Curr Biol 11:981-6. 2001
    ..Analysis of the spatio-temporal dynamics of CAPRI indicates that Ca(2+) regulates the GAP by a fast C2 domain-dependent translocation mechanism...
  15. ncbi request reprint Membrane targeting: what a difference a G makes
    P J Cullen
    Department of Biochemistry, University of Bristol, BS8 1TD, Bristol, UK
    Curr Biol 10:R876-8. 2000
    ....
  16. ncbi request reprint Insulin-dependent translocation of ARNO to the plasma membrane of adipocytes requires phosphatidylinositol 3-kinase
    K Venkateswarlu
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK
    Curr Biol 8:463-6. 1998
    ..Our data strongly suggest that ARNO binds PIP3 in vivo and that this interaction causes a translocation of ARNO to the plasma membrane where it might activate ARF6 and regulate subsequent plasma membrane cycling events...
  17. pmc Effects of elevated expression of inositol 1,4,5-trisphosphate 3-kinase B on Ca2+ homoeostasis in HeLa cells
    T H Millard
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochem J 352:709-15. 2000
    ..These data suggest that IP3KB may play a significant role in the regulation of Ins(1,4,5)P(3) levels, and consequently in Ca(2+) responses following stimulation of cells with Ins(1,4,5)P(3)-elevating agonists...
  18. ncbi request reprint The Ras binary switch: an ideal processor for decoding complex Ca2+ signals?
    S A Walker
    The Henry Wellcome Laboratories for Integrated Cell Signalling, Inositide Group, Department of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    Biochem Soc Trans 31:966-9. 2003
    ..Calcium-regulated GEFs and GAPs have been identified, some with an exquisite sensitivity to [Ca(2+)](i), implicating a potential role of complex calcium signals in regulating Ras...
  19. ncbi request reprint Distinct subcellular localisations of the putative inositol 1,3,4,5-tetrakisphosphate receptors GAP1IP4BP and GAP1m result from the GAP1IP4BP PH domain directing plasma membrane targeting
    P J Lockyer
    Laboratory of Molecular Studies on Cell Regulation, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Biol 7:1007-10. 1997
    ..This difference in localisation has fundamental significance for our understanding of the second messenger functions of IP4...
  20. ncbi request reprint The GAP1 family of GTPase-activating proteins: spatial and temporal regulators of small GTPase signalling
    S Yarwood
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochem Soc Trans 34:846-50. 2006
    ....
  21. ncbi request reprint Phospholipase Cgamma activates Ras on the Golgi apparatus by means of RasGRP1
    Trever G Bivona
    Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, New York 10016, USA
    Nature 424:694-8. 2003
    ..Thus, activation of Ras on Golgi has important biological consequences and proceeds through a pathway distinct from the one that activates Ras on the plasma membrane...
  22. pmc Epigenetic silencing of a Ca(2+)-regulated Ras GTPase-activating protein RASAL defines a new mechanism of Ras activation in human cancers
    Hongchuan Jin
    State Key Laboratory in Oncology in South China, Sir Y K Pao Center for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, Hong Kong
    Proc Natl Acad Sci U S A 104:12353-8. 2007
    ....
  23. ncbi request reprint Analyzing the role of the putative inositol 1,3,4,5-tetrakisphosphate receptor GAP1IP4BP in intracellular Ca2+ homeostasis
    Simon A Walker
    Department of Biochemistry, Inositide Group, Integrated Signalling Laboratories, School of Medical Sciences, University of Bristol, United Kingdom
    J Biol Chem 277:48779-85. 2002
    ..Thus, using various approaches to manipulate the function of endogenous GAP1(IP4BP) in intact HeLa cells, we have been unable to observe any detectable effect of GAP1(IP4BP) on [Ca(2+)](i)...
  24. pmc GAP1 family members constitute bifunctional Ras and Rap GTPase-activating proteins
    Sabine Kupzig
    Henry Wellcome Integrated Signaling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 281:9891-900. 2006
    ..Although GAP1(m) appears to constitute a specific Ras GAP, CAPRI and RASAL display dual activity. For CAPRI, its Rap GAP activity is modulated upon its Ca(2+)-induced association with the plasma membrane...
  25. pmc CAPRI and RASAL impose different modes of information processing on Ras due to contrasting temporal filtering of Ca2+
    Qing Liu
    Laboratory of Molecular Signaling, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, England, UK
    J Cell Biol 170:183-90. 2005
    ....
  26. pmc The frequencies of calcium oscillations are optimized for efficient calcium-mediated activation of Ras and the ERK/MAPK cascade
    Sabine Kupzig
    Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Proc Natl Acad Sci U S A 102:7577-82. 2005
    ..Our results describe a hitherto unrecognized link between complex Ca(2+) signals and the modulation of the Ras/ERK/MAPK signaling cascade...
  27. pmc Identification of a Ras GTPase-activating protein regulated by receptor-mediated Ca2+ oscillations
    Simon A Walker
    Inositide Group, Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, UK
    EMBO J 23:1749-60. 2004
    ..Thus, RASAL senses the frequency of complex Ca2+ signals, decoding them through a regulation of the activation state of Ras. Our data provide a hitherto unrecognised link between complex Ca2+ signals and the regulation of Ras...
  28. doi request reprint Endosomal sorting and signalling: an emerging role for sorting nexins
    Peter J Cullen
    Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD, UK
    Nat Rev Mol Cell Biol 9:574-82. 2008
    ..Here we discuss the sorting nexins family of proteins and propose that they have a fundamental role in orchestrating the formation of protein complexes that are involved in endosomal sorting and signalling...
  29. ncbi request reprint SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment
    Colin J Traer
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Nat Cell Biol 9:1370-80. 2007
    ..Finally, these data suggest that by associating with molecular motors, SNX-BAR proteins may coordinate sorting with carrier transport between donor and recipient membranes...
  30. ncbi request reprint Calcium signalling: the ups and downs of protein kinase C
    Peter J Cullen
    Inositide Group, Henry Wellcome Laboratories for Integrated Signalling, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Biol 13:R699-701. 2003
    ..In a recent study, advances in FRET technology have been used to describe how calcium oscillations are decoded through phase-locked oscillations in substrate phosphorylation catalysed by protein kinase C...
  31. ncbi request reprint Membrane curvature: the power of bananas, zeppelins and boomerangs
    Giles O C Cory
    Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Curr Biol 17:R455-7. 2007
    ..New work on the ability of IRSp53/MIM domains to induce negative membrane curvature sheds light on the mechanisms used to generate actin-rich cell protrusions...
  32. ncbi request reprint Control of Ras cycling by Ca2+
    Simon A Walker
    Inositide Group, Henry Wellcome Laboratories for Integrated Signalling, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    FEBS Lett 546:6-10. 2003
    ..These regulators of Ras cycling are likely to play a key role in the information processing of Ca(2+) and DAG signals...
  33. ncbi request reprint Studying the spatial and temporal regulation of Ras GTPase-activating proteins
    Sabine Kupzig
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, United Kingdom
    Methods Enzymol 407:64-82. 2006
    ..Thus, although each member of the GAP1 family performs the same basic biological function, that is, they function as Ras GAPs, each is designed to respond and decode signals from distinct second messenger pathways...
  34. ncbi request reprint Sorting nexin-1 mediates tubular endosome-to-TGN transport through coincidence sensing of high- curvature membranes and 3-phosphoinositides
    Jez Carlton
    Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    Curr Biol 14:1791-800. 2004
    ..SNX1 is associated with early endosomes, from where it has been proposed to regulate the degradation of internalized epidermal growth factor (EGF) receptors through modulating endosomal-to-lysosomal sorting...
  35. ncbi request reprint Engineering the phosphoinositide-binding profile of a class I pleckstrin homology domain
    Gyles E Cozier
    Inositide Group, Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom
    J Biol Chem 278:39489-96. 2003
    ....