P Cohen

Summary

Affiliation: University of Dundee
Country: UK

Publications

  1. pmc The phosphorylation of CapZ-interacting protein (CapZIP) by stress-activated protein kinases triggers its dissociation from CapZ
    Claire E Eyers
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 389:127-35. 2005
  2. pmc A novel UBA and UBX domain protein that binds polyubiquitin and VCP and is a substrate for SAPKs
    Helen McNeill
    MRC Protein Phosphorylation Unit, School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 384:391-400. 2004
  3. pmc Identification of calcium-regulated heat-stable protein of 24 kDa (CRHSP24) as a physiological substrate for PKB and RSK using KESTREL
    Gillian C Auld
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 389:775-83. 2005
  4. pmc Nogo-B is a new physiological substrate for MAPKAP-K2
    Simon Rousseau
    MRC Protein Phosphorylation Unit, Faculty of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 391:433-40. 2005
  5. pmc KESTREL: a powerful method for identifying the physiological substrates of protein kinases
    Philip Cohen
    MRC Protein Phosphorylation Unit, MSI WTB complex, Dow Street, Dundee DD1 5EH, UK
    Biochem J 393:1-6. 2006
  6. pmc The TLR and IL-1 signalling network at a glance
    Philip Cohen
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    J Cell Sci 127:2383-90. 2014
  7. pmc Polyubiquitin binding to ABIN1 is required to prevent autoimmunity
    Sambit K Nanda
    Medical Research Council Protein Phosphorylation Unit, Sir James Black Centre, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Exp Med 208:1215-28. 2011
  8. pmc The anti-inflammatory drug BAY 11-7082 suppresses the MyD88-dependent signalling network by targeting the ubiquitin system
    Sam Strickson
    MRC Protein Phosphorylation Unit, Sir James Black Centre, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 451:427-37. 2013
  9. pmc DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double-stranded RNA
    Alban Ordureau
    MRC Protein Phosphorylation and Ubiquitylation Unit, Sir James Black Centre, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 288:24569-80. 2013
  10. pmc Kinase drug discovery--what's next in the field?
    Philip Cohen
    MRC Protein Phosphorylation Unit, University of Dundee, Dundee DD1 5EH, United Kingdom
    ACS Chem Biol 8:96-104. 2013

Collaborators

Detail Information

Publications144 found, 100 shown here

  1. pmc The phosphorylation of CapZ-interacting protein (CapZIP) by stress-activated protein kinases triggers its dissociation from CapZ
    Claire E Eyers
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 389:127-35. 2005
    ..Osmotic shock or anisomycin triggered the dissociation of CapZIP from CapZ in Jurkat cells, suggesting that phosphorylation of CapZIP may regulate the ability of CapZ to remodel actin filament assembly in vivo...
  2. pmc A novel UBA and UBX domain protein that binds polyubiquitin and VCP and is a substrate for SAPKs
    Helen McNeill
    MRC Protein Phosphorylation Unit, School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 384:391-400. 2004
    ..We suggest that SAKS1 may be an adaptor that directs VCP to polyubiquitinated proteins, and PNGase to misfolded glycoproteins, facilitating their destruction by the proteasome...
  3. pmc Identification of calcium-regulated heat-stable protein of 24 kDa (CRHSP24) as a physiological substrate for PKB and RSK using KESTREL
    Gillian C Auld
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 389:775-83. 2005
    ....
  4. pmc Nogo-B is a new physiological substrate for MAPKAP-K2
    Simon Rousseau
    MRC Protein Phosphorylation Unit, Faculty of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 391:433-40. 2005
    ..The anisomycin-induced phosphorylation of Ser107 in HeLa cells can be prevented by 'knockdown' of MAPKAP-K2 using siRNA (small interfering RNA). Taken together, our results identify Nogo-B as a new physiological substrate of MAPKAP-K2...
  5. pmc KESTREL: a powerful method for identifying the physiological substrates of protein kinases
    Philip Cohen
    MRC Protein Phosphorylation Unit, MSI WTB complex, Dow Street, Dundee DD1 5EH, UK
    Biochem J 393:1-6. 2006
    ....
  6. pmc The TLR and IL-1 signalling network at a glance
    Philip Cohen
    MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    J Cell Sci 127:2383-90. 2014
    ..Finally, I suggest a few protein kinases that have been neglected as drug targets, but whose therapeutic potential should be explored in the light of recent advances in our understanding of their roles in the innate immune system. ..
  7. pmc Polyubiquitin binding to ABIN1 is required to prevent autoimmunity
    Sambit K Nanda
    Medical Research Council Protein Phosphorylation Unit, Sir James Black Centre, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Exp Med 208:1215-28. 2011
    ..The mutant B cells also proliferated more rapidly in response to TLR ligands. Our results indicate that the interaction of ABIN1 with polyubiquitin is required to limit the activation of TLR-MyD88 pathways and prevent autoimmunity...
  8. pmc The anti-inflammatory drug BAY 11-7082 suppresses the MyD88-dependent signalling network by targeting the ubiquitin system
    Sam Strickson
    MRC Protein Phosphorylation Unit, Sir James Black Centre, University of Dundee, Dundee DD1 5EH, UK
    Biochem J 451:427-37. 2013
    ....
  9. pmc DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double-stranded RNA
    Alban Ordureau
    MRC Protein Phosphorylation and Ubiquitylation Unit, Sir James Black Centre, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 288:24569-80. 2013
    ..DEAF1 is also needed for TLR3-dependent IFNβ production. Taken together, our results identify DEAF1 as a novel component of the signal transduction network by which dsRNA of viral origin stimulates IFNβ production. ..
  10. pmc Kinase drug discovery--what's next in the field?
    Philip Cohen
    MRC Protein Phosphorylation Unit, University of Dundee, Dundee DD1 5EH, United Kingdom
    ACS Chem Biol 8:96-104. 2013
    ....
  11. ncbi request reprint The role of protein phosphorylation in human health and disease. The Sir Hans Krebs Medal Lecture
    P Cohen
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 4HN, Scotland, UK
    Eur J Biochem 268:5001-10. 2001
    ..I also discuss the exploitation of specific protein kinase inhibitors for the study of cell signalling and make recommendations for their effective use in cell-based assays...
  12. ncbi request reprint The origins of protein phosphorylation
    Philip Cohen
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Scotland, UK
    Nat Cell Biol 4:E127-30. 2002
    ..Here I review the discovery of protein phosphorylation and give a personal view of the key findings that have helped to shape the field as we know it today...
  13. ncbi request reprint Protein kinases--the major drug targets of the twenty-first century?
    Philip Cohen
    Medical Research Council, Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Nat Rev Drug Discov 1:309-15. 2002
    ..Protein kinases have now become the second most important group of drug targets, after G-protein-coupled receptors. Here, I give a personal view of some of the most important advances that have shaped this field...
  14. ncbi request reprint GSK3 inhibitors: development and therapeutic potential
    Philip Cohen
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
    Nat Rev Drug Discov 3:479-87. 2004
  15. ncbi request reprint The renaissance of GSK3
    P Cohen
    MRC Protein Phosphorylation Unit and Division of Signal Transduction Therapy, School of Life Sciences, University of Dundee, DD1 5EH, Scotland, UK
    Nat Rev Mol Cell Biol 2:769-76. 2001
    ..The study of the substrate specificity and regulation of GSK3 activity has been important in the quest for therapeutic intervention...
  16. ncbi request reprint The twentieth century struggle to decipher insulin signalling
    Philip Cohen
    Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, The Sir James Black Centre, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Nat Rev Mol Cell Biol 7:867-73. 2006
    ..What are the main discoveries that led to our current understanding of this process? And how is this new knowledge being exploited in an attempt to develop improved drugs to treat the epidemic of type-2 diabetes?..
  17. doi request reprint Targeting protein kinases for the development of anti-inflammatory drugs
    Philip Cohen
    The MRC Protein Phosphorylation Unit, University of Dundee, The Sir James Black Centre, Dundee, UK
    Curr Opin Cell Biol 21:317-24. 2009
    ....
  18. doi request reprint Guidelines for the effective use of chemical inhibitors of protein function to understand their roles in cell regulation
    Philip Cohen
    MRC Protein Phosphorylation Unit, The Sir James Black Centre, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 425:53-4. 2010
    ..In the present paper, using protein kinase inhibitors as the example, a set of criteria are described that should be met before any investigation using such compounds should be accepted for publication...
  19. doi request reprint Will the ubiquitin system furnish as many drug targets as protein kinases?
    Philip Cohen
    Sir James Black Centre, Dundee, Scotland, UK
    Cell 143:686-93. 2010
    ..In this Perspective, we discuss the reasons for the delay in this pipeline, the drugs targeting the ubiquitin system that have been developed, and new approaches that may popularize this area of drug discovery in the future...
  20. pmc Roles of the forkhead in rhabdomyosarcoma (FKHR) phosphorylation sites in regulating 14-3-3 binding, transactivation and nuclear targetting
    G Rena
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 354:605-12. 2001
    ....
  21. ncbi request reprint SKK4, a novel activator of stress-activated protein kinase-1 (SAPK1/JNK)
    S Lawler
    Department of Biochemistry, University of Dundee, UK
    FEBS Lett 414:153-8. 1997
    ..The identification of SKK4 explains why the major SAPK1/JNK activator detected in many mammalian cell extracts is chromatographically separable from SKK1/MKK4...
  22. ncbi request reprint Further evidence that the inhibition of glycogen synthase kinase-3beta by IGF-1 is mediated by PDK1/PKB-induced phosphorylation of Ser-9 and not by dephosphorylation of Tyr-216
    M Shaw
    Department of Biochemistry, University of Dundee, UK
    FEBS Lett 416:307-11. 1997
    ..Coexpression of WT-GSK3beta in 293 cells with either PKB alpha (also known as AKT) or PDK1 (the 'upstream' activator of PKB) mimicked the IGF-1- or insulin-induced phosphorylation of Ser-9 and inactivation of GSK3beta...
  23. ncbi request reprint Role of mitogen-activated protein kinase cascades in mediating lipopolysaccharide-stimulated induction of cyclooxygenase-2 and IL-1 beta in RAW264 macrophages
    M Caivano
    Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee, United Kingdom
    J Immunol 164:3018-25. 2000
    ..They also suggest that MSK1 and MSK2 may play a role in these processes and hence are potential targets for the development of novel antiinflammatory drugs...
  24. ncbi request reprint A myofibrillar protein phosphatase from rabbit skeletal muscle contains the beta isoform of protein phosphatase-1 complexed to a regulatory subunit which greatly enhances the dephosphorylation of myosin
    P Dent
    Department of Biochemistry, The University, Dundee, Scotland
    Eur J Biochem 210:1037-44. 1992
    ....
  25. ncbi request reprint Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha
    D R Alessi
    Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, Scotland
    Curr Biol 7:261-9. 1997
    ..The phosphorylation of both residues is required for maximal activation of PKB. The kinases that phosphorylate PKB are, however, unknown...
  26. ncbi request reprint PPP1R6, a novel member of the family of glycogen-targetting subunits of protein phosphatase 1
    C G Armstrong
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 418:210-4. 1997
    ..The PPP1R6 is associated with glycogen in muscle but is not subject to the same modes of covalent and allosteric regulation as G(M) and G(L)...
  27. pmc Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7
    Y Fleming
    MRC Protein Phosphorylation Unit, Department of Biochemistry, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 352:145-54. 2000
    ..MKK7 also phosphorylates SAPK2a/p38 at a low rate (but not SAPK3/p38 gamma or SAPK4/p38 delta), and phosphorylation occurs exclusively at the tyrosine residue, demonstrating that MKK7 is intrinsically a 'dual-specific' protein kinase...
  28. pmc Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase
    T Kobayashi
    MRC Protein Phosphorylation Unit, Department of Biochemistry, MSI WTB complex, Dow Street, University of Dundee, Dundee DD1 5EH, Scotland, U K
    Biochem J 344:189-97. 1999
    ..Like PKB and SGK1, SGK2 and SGK3 preferentially phosphorylate Ser and Thr residues that lie in Arg-Xaa-Arg-Xaa-Xaa-Ser/Thr motifs...
  29. pmc MAPKAP kinase-2; a novel protein kinase activated by mitogen-activated protein kinase
    D Stokoe
    Department of Biochemistry, University of Dundee, UK
    EMBO J 11:3985-94. 1992
    ..These results indicate that MAP kinase activates at least two distinct protein kinases, suggesting that it represents a point at which the growth factor-stimulated protein kinase cascade bifurcates...
  30. ncbi request reprint Conversion of SB 203580-insensitive MAP kinase family members to drug-sensitive forms by a single amino-acid substitution
    P A Eyers
    Department of Biochemistry, University of Dundee, UK
    Chem Biol 5:321-8. 1998
    ....
  31. ncbi request reprint Stress-activated protein kinase-2/p38 and a rapamycin-sensitive pathway are required for C2C12 myogenesis
    A Cuenda
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, United Kingdom
    J Biol Chem 274:4341-6. 1999
    ..These results show for the first time that SAPK2/p38 plays an essential role in C2C12 cell differentiation...
  32. ncbi request reprint Purification of the hepatic glycogen-associated form of protein phosphatase-1 by microcystin-Sepharose affinity chromatography
    G Moorhead
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 362:101-5. 1995
    ..The GL subunit binds phosphorylase a with high affinity, and is responsible for the enhanced dephosphorylation of glycogen synthase by PP1G and its allosteric inhibition by phosphorylase a...
  33. ncbi request reprint Participation of a stress-activated protein kinase cascade in the activation of tyrosine hydroxylase in chromaffin cells
    G Thomas
    Department of Biochemistry, University of Dundee, Scotland
    Eur J Biochem 247:1180-9. 1997
    ..SAPK2 may therefore contribute to the acetylcholine-induced activation of tyrosine hydroxylase by facilitating (in an unknown way) its phosphorylation by MAP kinases...
  34. pmc Purification and cDNA cloning of SAPKK3, the major activator of RK/p38 in stress- and cytokine-stimulated monocytes and epithelial cells
    A Cuenda
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, UK
    EMBO J 15:4156-64. 1996
    ..The murine and human SAPKK3 were 97% identical in their amino acid sequences. We also cloned a different murine cDNA that appears to encode a SAPKK3 protein truncated at the N-terminus. SAPKK3 is identical to the recently cloned MKK6...
  35. pmc The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site
    Y L Woods
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 355:597-607. 2001
    ..We find that FKHR and DYRK1A co-localize in discrete regions of the nucleus and can be co-immunoprecipitated from cell extracts. These experiments suggest that DYRK1A may phosphorylate FKHR at Ser(329) in vivo...
  36. ncbi request reprint Nuclear organisation of NIPP1, a regulatory subunit of protein phosphatase 1 that associates with pre-mRNA splicing factors
    L Trinkle-Mulcahy
    Department of Biochemistry, The University, MSI WTB complex, Dow Street, Dundee DD1 5EH, Scotland, UK
    J Cell Sci 112:157-68. 1999
    ..These findings implicate the NIPP1-PP1 complex in the control of pre-mRNA splicing...
  37. ncbi request reprint The major myosin phosphatase in skeletal muscle is a complex between the beta-isoform of protein phosphatase 1 and the MYPT2 gene product
    G Moorhead
    Department of Biochemistry, University of Dundee, UK
    FEBS Lett 438:141-4. 1998
    ..Surprisingly, the M21 subunit of smPP1M appears to be produced from the same gene that encodes MYPT2...
  38. pmc Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1
    D R Alessi
    Department of Biochemistry, University of Dundee, Scotland
    EMBO J 13:1610-9. 1994
    ....
  39. ncbi request reprint Identification of regulatory phosphorylation sites in mitogen-activated protein kinase (MAPK)-activated protein kinase-1a/p90rsk that are inducible by MAPK
    K N Dalby
    Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Scotland, United Kingdom
    J Biol Chem 273:1496-505. 1998
    ..Ser222, Ser364, and Ser381 are situated in analogous positions to phosphorylation sites in protein kinase B, protein kinase C, and p70S6K, suggesting a common mechanism of activation for these growth factor-stimulated protein kinases...
  40. pmc Activation of stress-activated protein kinase-3 (SAPK3) by cytokines and cellular stresses is mediated via SAPKK3 (MKK6); comparison of the specificities of SAPK3 and SAPK2 (RK/p38)
    A Cuenda
    Department of Biochemistry, University of Dundee, UK
    EMBO J 16:295-305. 1997
    ..Our results suggest that cellular functions previously attributed to SAPK1 and/or SAPK2 may be mediated by SAPK3...
  41. pmc Stress-induced regulation of eukaryotic elongation factor 2 kinase by SB 203580-sensitive and -insensitive pathways
    Axel Knebel
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 367:525-32. 2002
    ..Since the phosphorylation of Ser-377 does not inhibit eEF2 kinase in vitro, our results suggest that anisomycin or TNF-alpha inhibit eEF2 kinase via the phosphorylation of Ser-359...
  42. ncbi request reprint The role of 3-phosphoinositide-dependent protein kinase 1 in activating AGC kinases defined in embryonic stem cells
    M R Williams
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dundee, DD1 5EH, Scotland
    Curr Biol 10:439-48. 2000
    ....
  43. pmc The activation of protein kinase B by H2O2 or heat shock is mediated by phosphoinositide 3-kinase and not by mitogen-activated protein kinase-activated protein kinase-2
    M Shaw
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, UK
    Biochem J 336:241-6. 1998
    ..The activation of PKB isoforms by H2O2 may underlie some of the insulin-mimetic effects of this compound...
  44. pmc The kinase DYRK phosphorylates protein-synthesis initiation factor eIF2Bepsilon at Ser539 and the microtubule-associated protein tau at Thr212: potential role for DYRK as a glycogen synthase kinase 3-priming kinase
    Y L Woods
    MRC Protein Phosphorylation Unit, School of Life Sciences, MSI WTB complex, Dow Street, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 355:609-15. 2001
    ..Phosphorylation of Thr(212) primes tau for phosphorylation by GSK3 at Ser(208) in vitro, suggesting a more general role for DYRK isoforms in priming phosphorylation of GSK3 substrates...
  45. pmc Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2
    T Kobayashi
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 339:319-28. 1999
    ..These findings raise the possibility that some physiological roles ascribed to PKB on the basis of the overexpression of constitutively active PKB mutants might be mediated by SGK...
  46. ncbi request reprint Phosphorylation of the skeletal muscle glycogen-targetting subunit of protein phosphatase 1 in response to adrenaline in vivo
    K S Walker
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee, UK
    FEBS Lett 466:121-4. 2000
    ..Thus the phosphorylation of G(M) at Ser48 by MAPKAP-K1 or other insulin-stimulated protein kinases is not involved in the activation of glycogen synthase by insulin...
  47. ncbi request reprint Regulation of protein phosphatase-1G from rabbit skeletal muscle. 1. Phosphorylation by cAMP-dependent protein kinase at site 2 releases catalytic subunit from the glycogen-bound holoenzyme
    M J Hubbard
    Department of Biochemistry, University of Dundee, Scotland
    Eur J Biochem 186:701-9. 1989
    ....
  48. pmc Mechanism of activation of protein kinase B by insulin and IGF-1
    D R Alessi
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, UK
    EMBO J 15:6541-51. 1996
    ..We propose a model whereby PKBalpha becomes phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s)...
  49. ncbi request reprint Multisite phosphorylation of the glycogen-binding subunit of protein phosphatase-1G by cyclic AMP-dependent protein kinase and glycogen synthase kinase-3
    P Dent
    Department of Biochemistry, Medical Sciences Institute, University of Dundee, Scotland
    FEBS Lett 248:67-72. 1989
    ....
  50. pmc Feedback control of the protein kinase TAK1 by SAPK2a/p38alpha
    Peter C F Cheung
    MRC Protein Phosphorylation Unit, School of Life Sciences, MSI WTB complex, Dow Street, University of Dundee, Dundee DD1 5EH, UK
    EMBO J 22:5793-805. 2003
    ....
  51. ncbi request reprint Phosphorylation of the transcription factor forkhead family member FKHR by protein kinase B
    G Rena
    Department of Biochemistry, Medical Research Council Protein Phosphorylation Unit, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 274:17179-83. 1999
    ..The conservation of Thr-24, Ser-256, and Ser-319 and the sequences surrounding them in DAF16 therefore suggests that DAF16 is also a direct substrate for PKB in C. elegans...
  52. ncbi request reprint Effects of MAP kinase cascade inhibitors on the MKK5/ERK5 pathway
    N Mody
    Medical Research Council Protein Phosphorylation Unit, School of Life Sciences, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, UK
    FEBS Lett 502:21-4. 2001
    ..Our results also indicate that ERK5 is not a significant activator of MAPK-activated protein kinase-1/RSK in HeLa cells...
  53. pmc Regulation of BAD by cAMP-dependent protein kinase is mediated via phosphorylation of a novel site, Ser155
    J M Lizcano
    MRC Protein Phosphorylation Unit, Department of Biochemistry, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 349:547-57. 2000
    ....
  54. ncbi request reprint Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B
    D A Cross
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, UK
    Nature 378:785-9. 1995
    ..Like the inhibition of GSK3 (refs 10, 14), the activation of PKB is prevented by inhibitors of phosphatidylinositol (PI) 3-kinase...
  55. ncbi request reprint Further evidence that inhibitor-2 acts like a chaperone to fold PP1 into its native conformation
    C Mackintosh
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, UK
    FEBS Lett 397:235-8. 1996
    ....
  56. pmc Role of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1
    R A Currie
    Department of Biochemistry, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 337:575-83. 1999
    ..Thirdly, the interaction of PDK1 with PtdIns(3,4,5)P3 facilitates the rate at which it can activate PKB...
  57. ncbi request reprint Paradoxical activation of Raf by a novel Raf inhibitor
    C A Hall-Jackson
    MRC Protein Phosphorylation Unit, Department of Biochemistry, MSI WTB complex, The University of Dundee, Dow Street, Dundee, DD1 5EH, UK
    Chem Biol 6:559-68. 1999
    ..Raf is a proto-oncogene that is activated in response to growth factors or phorbol esters, and is thought to activate MAP kinase kinase-1 (MKK1) and hence the classical MAP kinase (MAPK) cascade...
  58. ncbi request reprint The control of protein phosphatase-1 by targetting subunits. The major myosin phosphatase in avian smooth muscle is a novel form of protein phosphatase-1
    D Alessi
    Department of Biochemistry, University of Dundee, Scotland
    Eur J Biochem 210:1023-35. 1992
    ....
  59. pmc The substrate specificity and structure of mitogen-activated protein (MAP) kinase-activated protein kinase-2
    D Stokoe
    Department of Biochemistry, University of Dundee, Scotland, UK
    Biochem J 296:843-9. 1993
    ..The threonine residue phosphorylated by MAP kinase lies immediately C-terminal to the catalytic domain and is followed by a nuclear localization signal, Lys-Lys-(Xaa)10-Lys-Arg-Arg-Lys-Lys, near the C-terminus...
  60. ncbi request reprint MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells
    J S Arthur
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    FEBS Lett 482:44-8. 2000
    ..Thus MSK1 is required for CREB and ATF1 phosphorylation after mitogenic stimulation of ES cells...
  61. ncbi request reprint A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins
    J Rouse
    Department of Biochemistry, University of Dundee, Scotland
    Cell 78:1027-37. 1994
    ..The RKK, RK, and MAPKAP kinase-2 constitute a new stress-activated signal transduction pathway in vertebrates that is distinct from the classical MAPK cascade...
  62. pmc A novel method to identify protein kinase substrates: eEF2 kinase is phosphorylated and inhibited by SAPK4/p38delta
    A Knebel
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    EMBO J 20:4360-9. 2001
    ..The phosphorylation of eEF2K at Ser359 was also induced by insulin-like growth factor-1. However, this was blocked by rapamycin, indicating that Ser359 is targeted by at least two signalling pathways...
  63. ncbi request reprint Identification of the 38-kDa subunit of rabbit skeletal muscle glycogen synthase as glycogenin
    J Pitcher
    Department of Biochemistry, University of Dundee, Scotland
    Eur J Biochem 169:497-502. 1987
    ..This demonstrated that the 38-kDa subunit was glycogenin, the protein that is bound covalently to glycogen and believed to be the 'primer' involved in the initiation of de novo glycogen synthesis...
  64. ncbi request reprint Activation of the neutrophil NADPH oxidase is inhibited by SB 203580, a specific inhibitor of SAPK2/p38
    A S Lal
    Department of Biochemistry, University College London, Dundee, DD1 5EH, United Kingdom
    Biochem Biophys Res Commun 259:465-70. 1999
    ..SB 203580 does not exert its effect by preventing the neutrophil priming reaction, by suppressing the phosphorylation of p47phax, or by preventing the translocation of p47phax/p67phax to the plasma membrane...
  65. ncbi request reprint The induction of cyclooxygenase-2 mRNA in macrophages is biphasic and requires both CCAAT enhancer-binding protein beta (C/EBP beta ) and C/EBP delta transcription factors
    M Caivano
    Medical Research Council Protein Phosphorylation Unit, University of Dundee, Dundee DD1 5EH, Scotland
    J Biol Chem 276:48693-701. 2001
    ..Taken together, these data identify CREB, NF-kappa B, and both C/EBP beta and -delta as key factors in coordinately orchestrating transcription from the COX-2 promoter in activated macrophages...
  66. ncbi request reprint Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: homology with protein phosphatase 2A
    N Berndt
    Department of Biochemistry, University of Dundee, Scotland
    FEBS Lett 223:340-6. 1987
    ..The first 10 and last 31 residues were dissimilar. Residues 1-101 of the type-1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases...
  67. ncbi request reprint A common phosphate binding site explains the unique substrate specificity of GSK3 and its inactivation by phosphorylation
    S Frame
    Division of Signal Transduction Therapy, School of Life Sciences, University of Dundee, DD1 5EH, Dundee, United Kingdom
    Mol Cell 7:1321-7. 2001
    ....
  68. ncbi request reprint Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells
    Y N Doza
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 364:223-8. 1995
    ..Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phosphorylation of both residues is required for the activation of this enzyme...
  69. ncbi request reprint IL-1beta-stimulated activation of ERK1/2 and p38alpha MAPK mediates the transcriptional up-regulation of IL-6, IL-8 and GRO-alpha in HeLa cells
    Huei Ting Yang
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Sir James Black Centre, Dow Street, Dundee, DD1 5EH, United Kingdom
    Cell Signal 20:375-80. 2008
    ....
  70. pmc Use of the pharmacological inhibitor BX795 to study the regulation and physiological roles of TBK1 and IkappaB kinase epsilon: a distinct upstream kinase mediates Ser-172 phosphorylation and activation
    Kristopher Clark
    MRC Protein Phosphorylation Unit, College of Life Sciences, Sir James Black Centre, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 284:14136-46. 2009
    ....
  71. ncbi request reprint Alternative splicing regulates the production of ARD-1 endoribonuclease and NIPP-1, an inhibitor of protein phosphatase-1, as isoforms encoded by the same gene
    A C Chang
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee, UK
    Gene 240:45-55. 1999
    ..Our results establish the process by which functionally disparate ARD-1 and NIPP-1 peptides are generated from the protein-coding sequence of the same gene in human cells...
  72. ncbi request reprint A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress
    A D Clifton
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 392:209-14. 1996
    ..These results establish that MAPKAP kinase-3 lies 'downstream' of SAPK-2 and that it is likely to have overlapping or identical substrates to MAPKAP kinase-2 in vivo...
  73. ncbi request reprint MAP kinase kinase from rabbit skeletal muscle. A novel dual specificity enzyme showing homology to yeast protein kinases involved in pheromone-dependent signal transduction
    S Nakielny
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 308:183-9. 1992
    ....
  74. ncbi request reprint Amino acid sequence and expression of the hepatic glycogen-binding (GL)-subunit of protein phosphatase-1
    M J Doherty
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 375:294-8. 1995
    ..The cDNA was expressed in E. coli, and the expressed protein transformed the properties of PP1 to those characteristic of the hepatic glycogen-associated enzyme. These experiments establish that the cloned protein is GL...
  75. ncbi request reprint The molecular mechanism by which insulin stimulates glycogen synthesis in mammalian skeletal muscle
    P Dent
    Department of Biochemistry, University of Dundee, Scotland, UK
    Nature 348:302-8. 1990
    ..Furthermore, the phosphorylation of this residue is stimulated by insulin in vivo. Increased and decreased phosphorylation of proteins by insulin can therefore be explained through the same basic underlying mechanism...
  76. ncbi request reprint The development and therapeutic potential of protein kinase inhibitors
    P Cohen
    MRC Protein Phosphorylation Unit, Department of Biochemistry, MSI WTB complex, Dow Street, University of Dundee, Dundee, DD1 5EH, Scotland, UK
    Curr Opin Chem Biol 3:459-65. 1999
    ..The basis for the specificity of several of these drugs has been elucidated and a number are undergoing human clinical trials...
  77. ncbi request reprint Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M
    Y H Chen
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 356:51-5. 1994
    ..The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit...
  78. ncbi request reprint Remarkable similarities between yeast and mammalian protein phosphatases
    P Cohen
    Department of Biochemistry, University of Dundee, Scotland
    FEBS Lett 250:601-6. 1989
    ..250,000,000] may be applicable to all eukaryotic cells...
  79. ncbi request reprint Signalling pathways involved in multisite phosphorylation of the transcription factor ATF-2
    Simon Morton
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dundee DD1 5EH, Scotland, UK
    FEBS Lett 572:177-83. 2004
    ..In JNK-deficient cells, p38 MAPK substituted for JNK partially in the phosphorylation of Thr69 and p38 MAPK or ERK1/2 in the phosphorylation of Thr71. JNK was the only MAP kinase that phosphorylated Ser90 under the conditions examined...
  80. ncbi request reprint Phosphorylation of Ewing's sarcoma protein (EWS) and EWS-Fli1 in response to DNA damage
    Iva V Klevernic
    MRC Protein Phosphorylation Unit, The Sir James Black Centre, University of Dundee, Dundee, Scotland, U K
    Biochem J 418:625-34. 2009
    ..In contrast, the phosphorylation of EWS-Fli1 induced by MMS was largely mediated by p38alpha/p38beta MAPKs. MMS induced a much stronger phosphorylation of EWS-Fli1 than EWS in heterodimers comprising both proteins...
  81. pmc MSK1 and MSK2 are required for the mitogen- and stress-induced phosphorylation of CREB and ATF1 in fibroblasts
    Giselle R Wiggin
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland
    Mol Cell Biol 22:2871-81. 2002
    ..The transcription of egr1 in response to both mitogenic and stress stimuli, as well as stress-induced apoptosis, was unaffected in the MSK1/MSK2 double knockout...
  82. ncbi request reprint Identification of a phosphorylation site on skeletal muscle myosin light chain kinase that becomes phosphorylated during muscle contraction
    Claire E Haydon
    MRC Protein Phosphorylation Unit, University of Dundee, Dundee, DD1 5EH, Scotland, UK
    Arch Biochem Biophys 397:224-31. 2002
    ..The sequence surrounding Ser(161) is unusual in that residue 158 is histidine. Previously, an arginine located three residues N-terminal to the site of phosphorylation was thought to be critical for the specificity of MAPKAP-K2...
  83. ncbi request reprint Phosphorylation and activation of human tyrosine hydroxylase in vitro by mitogen-activated protein (MAP) kinase and MAP-kinase-activated kinases 1 and 2
    C Sutherland
    Department of Biochemistry, University of Dundee, Scotland
    Eur J Biochem 217:715-22. 1993
    ..The effect on activity of phosphorylating both Ser31 and Ser40 was not additive. The possible roles of MAPKAP kinase-1, MAPKAP kinase-2 and MAP kinase in the regulation of tyrosine hydroxylase in vivo are discussed...
  84. ncbi request reprint Mechanism of activation and function of protein kinase B
    D R Alessi
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, UK
    Curr Opin Genet Dev 8:55-62. 1998
    ..The highlights include the discovery of a protein kinase required for the 3-phosphoinositide-dependent activation of PKB and the identification of several physiological substrates for PKB...
  85. pmc Phosphorylation of the ARE-binding protein DAZAP1 by ERK2 induces its dissociation from DAZ
    Simon Morton
    MRC Protein Phosphorylation Unit, Faculty of Life Sciences, University of Dundee, CIR Building, Dow Street, Dundee DD1 5EH, UK
    Biochem J 399:265-73. 2006
    ....
  86. pmc A non-radioactive method for the assay of many serine/threonine-specific protein kinases
    Heike Ross
    Division of Signal Transduction Therapy, School of Life Sciences, University of Dundee, Scotland, UK
    Biochem J 366:977-81. 2002
    ..Thus a large number of serine/threonine-specific protein kinases can be screened using a simple non-radioactive format...
  87. ncbi request reprint Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins
    D Stokoe
    Department of Biochemistry, University of Dundee, Scotland, UK
    FEBS Lett 313:307-13. 1992
    ..These results suggest that MAPKAP kinase-2 is the enzyme responsible for the phosphorylation of these small heat shock proteins in mammalian cells...
  88. pmc Molecular mechanisms involved in the regulation of cytokine production by muramyl dipeptide
    Mark Windheim
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 404:179-90. 2007
    ....
  89. pmc The selectivity of protein kinase inhibitors: a further update
    Jenny Bain
    Division of Signal Transduction Therapy, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 408:297-315. 2007
    ..Despite being used widely, many of the compounds that we analysed were too non-specific for useful conclusions to be made, other than to exclude the involvement of particular protein kinases in cellular processes...
  90. ncbi request reprint Synthetic anisomycin analogues activating the JNK/SAPK1 and p38/SAPK2 pathways
    Edward M Rosser
    MRC Protein Phosphorylation Unit, Division of Cell Signalling, School of Life Sciences, University of Dundee, Dundee, UKDD1 5EH
    Org Biomol Chem 2:142-9. 2004
    ..These studies demonstrate that some of the synthetic C(4) analogues are also potent activators of these stress kinase pathways...
  91. pmc The specificities of protein kinase inhibitors: an update
    Jenny Bain
    Division of Signal Transduction Therapy, School of Life Sciences, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 371:199-204. 2003
    ..33 microM) and p38-regulated/activated kinase (PRAK; IC(50)=1.0 microM)...
  92. pmc Characterization of a novel phosphatidylinositol 3-phosphate-binding protein containing two FYVE fingers in tandem that is targeted to the Golgi
    P C Cheung
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, U K
    Biochem J 355:113-21. 2001
    ..Cell localization studies using a green fluorescent protein fusion show that TAFF1 is localized to the Golgi, and that the Golgi targeting sequence is located within the N-terminal 187 residues and not in either FYVE domain...
  93. ncbi request reprint The regulation of protein function by multisite phosphorylation--a 25 year update
    P Cohen
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dundee, UK DD1 5EH
    Trends Biochem Sci 25:596-601. 2000
    ..Multisite phosphorylation can enable several such effects to operate in the same protein. It can also determine the extent and duration of a response and is the key to signal integration...
  94. ncbi request reprint An essential role for calmodulin in regulating human T cell aggregation
    S C Fagerholm
    Medical Research Council Protein Phosphorylation Unit, University of Dundee, Dundee DD1 5EH, UK
    FEBS Lett 491:131-6. 2001
    ....
  95. pmc Activation of protein kinase B beta and gamma isoforms by insulin in vivo and by 3-phosphoinositide-dependent protein kinase-1 in vitro: comparison with protein kinase B alpha
    K S Walker
    MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, U K
    Biochem J 331:299-308. 1998
    ..Insulin did not induce PKBgamma activity in adipocytes, hepatocytes or skeletal muscle, but PKBgamma was the major isoform activated by insulin in rat L6 myotubes (a skeletal-muscle cell line)...
  96. ncbi request reprint Engineering protein kinases with distinct nucleotide specificities and inhibitor sensitivities by mutation of a single amino acid
    P Cohen
    MRC Protein Phosphorylation Unit Department of Biochemistry University of Dundee MSI WTB Complex Dow Street, Dundee, DD1 5EH, UK
    Chem Biol 5:R161-4. 1998
    ..The task might be simplified by the discovery that the mutation of a single amino acid dramatically alters the nucleotide specificity of protein kinases and their inhibition by a particular class of anti-inflammatory drug...
  97. pmc Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs
    Simon Rousseau
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, MSI WTB complex, Dow Street, Dundee DD1 5EH, UK E mail
    EMBO J 21:6505-14. 2002
    ..Our data suggest that LPS-induced binding of hnRNP A0 to AREs may contribute to the post-transcriptional regulation of specific mRNAs...
  98. ncbi request reprint CXCL12 and C5a trigger cell migration via a PAK1/2-p38alpha MAPK-MAPKAP-K2-HSP27 pathway
    Simon Rousseau
    MRC Protein Phosphorylation Unit, Faculty of Life Sciences, University of Dundee, CIR Building, Dow Street, Dundee DD1 5EH, United Kingdom
    Cell Signal 18:1897-905. 2006
    ..These results demonstrate a general and essential role of the PAK-p38alpha MAPK-MAPKAP-K2-HSP27 signalling pathway in mediating the effects of chemotactic stimuli on cell migration...
  99. pmc Characterization of the reversible phosphorylation and activation of ERK8
    Iva V Klevernic
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 394:365-73. 2006
    ....
  100. ncbi request reprint Roles for TAB1 in regulating the IL-1-dependent phosphorylation of the TAB3 regulatory subunit and activity of the TAK1 complex
    Heidi Mendoza
    MRC Protein Phosphorylation Unit, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 409:711-22. 2008
    ....
  101. pmc A reinvestigation of the multisite phosphorylation of the transcription factor c-Jun
    Simon Morton
    MRC Protein Phosphorylation Unit, MSI WTB complex, University of Dundee, Dundee DD1 5EH, UK
    EMBO J 22:3876-86. 2003
    ..The agonist-induced dephosphorylation of Thr239 may involve a conformational change that exposes Thr239 to dephosphorylation and/or the activation of a Thr239 phosphatase...