Aled Clayton

Summary

Affiliation: University Hospital of Wales
Country: UK

Publications

  1. ncbi request reprint Exosomes and the MICA-NKG2D system in cancer
    Aled Clayton
    Department of Clinical Oncology and Palliative Medicine, Wales College of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff CF14 2TL, UK
    Blood Cells Mol Dis 34:206-13. 2005
  2. ncbi request reprint Adhesion and signaling by B cell-derived exosomes: the role of integrins
    Aled Clayton
    Section of Clinical Oncology, Department of Medicine, University of Wales College of Medicine, Velindre Hospital, Whitchurch, Cardiff, UK
    FASEB J 18:977-9. 2004
  3. ncbi request reprint Antigen-presenting cell exosomes are protected from complement-mediated lysis by expression of CD55 and CD59
    Aled Clayton
    Section of Clinical Oncology and Palliative Medicine, Department of Medicine, University of Wales College of Medicine, Velindre Hospital, Whitchurch, Cardiff, GB
    Eur J Immunol 33:522-31. 2003
  4. ncbi request reprint Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2
    Aled Clayton
    Department of Oncology and Palliative Medicine, School of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, United Kingdom
    Cancer Res 67:7458-66. 2007
  5. ncbi request reprint Induction of heat shock proteins in B-cell exosomes
    Aled Clayton
    Department of Clinical Oncology and Palliative Medicine, Wales College of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, CF14 2TL, UK
    J Cell Sci 118:3631-8. 2005
  6. ncbi request reprint Human tumor-derived exosomes down-modulate NKG2D expression
    Aled Clayton
    Department of Oncology and Palliative Medicine, School of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, United Kingdom
    J Immunol 180:7249-58. 2008
  7. ncbi request reprint Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry
    A Clayton
    Section of Clinical Oncology, University of Wales College of Medicine, Velindre Hospital, Whitchurch, Cardiff CF14 2TL, UK
    J Immunol Methods 247:163-74. 2001
  8. doi request reprint Resistance of CD45RA- T cells to apoptosis and functional impairment, and activation of tumor-antigen specific T cells during radiation therapy of prostate cancer
    Zsuzsanna Tabi
    Department of Pharmacology, Radiology and Oncology, School of Medicine, Cardiff University, Cancer Research Wales Research Laboratories, Velindre Cancer Centre, Whitchurch, Cardiff, CF14 2TL, UK
    J Immunol 185:1330-9. 2010
  9. doi request reprint Increased exosome production from tumour cell cultures using the Integra CELLine Culture System
    J Paul Mitchell
    Department of Oncology and Palliative Medicine, School of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, UK
    J Immunol Methods 335:98-105. 2008
  10. doi request reprint Cancer exosomes trigger fibroblast to myofibroblast differentiation
    Jason Webber
    Department of Pharmacology, Oncology and Radiation, School of Medicine, Cardiff University, Cardiff, UK
    Cancer Res 70:9621-30. 2010

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Exosomes and the MICA-NKG2D system in cancer
    Aled Clayton
    Department of Clinical Oncology and Palliative Medicine, Wales College of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff CF14 2TL, UK
    Blood Cells Mol Dis 34:206-13. 2005
    ..These data highlight a role for tumor exosomes bearing NKG2D ligands as a mechanism contributing to cancer immune evasion...
  2. ncbi request reprint Adhesion and signaling by B cell-derived exosomes: the role of integrins
    Aled Clayton
    Section of Clinical Oncology, Department of Medicine, University of Wales College of Medicine, Velindre Hospital, Whitchurch, Cardiff, UK
    FASEB J 18:977-9. 2004
    ....
  3. ncbi request reprint Antigen-presenting cell exosomes are protected from complement-mediated lysis by expression of CD55 and CD59
    Aled Clayton
    Section of Clinical Oncology and Palliative Medicine, Department of Medicine, University of Wales College of Medicine, Velindre Hospital, Whitchurch, Cardiff, GB
    Eur J Immunol 33:522-31. 2003
    ..We conclude that exosomes express GPI-anchored complement regulators in order to permit their survival in the extracellular environment...
  4. ncbi request reprint Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2
    Aled Clayton
    Department of Oncology and Palliative Medicine, School of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, United Kingdom
    Cancer Res 67:7458-66. 2007
    ..This coordinated "double hit" to cellular immunity strongly implicates the role of exosomes in tumor immune evasion...
  5. ncbi request reprint Induction of heat shock proteins in B-cell exosomes
    Aled Clayton
    Department of Clinical Oncology and Palliative Medicine, Wales College of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, CF14 2TL, UK
    J Cell Sci 118:3631-8. 2005
    ....
  6. ncbi request reprint Human tumor-derived exosomes down-modulate NKG2D expression
    Aled Clayton
    Department of Oncology and Palliative Medicine, School of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, United Kingdom
    J Immunol 180:7249-58. 2008
    ..This hyporesponsiveness was evident even in the presence of IL-15, a strong inducer of NKG2D. Our data show that NKG2D is a likely physiological target for exosome-mediated immune evasion in cancer...
  7. ncbi request reprint Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry
    A Clayton
    Section of Clinical Oncology, University of Wales College of Medicine, Velindre Hospital, Whitchurch, Cardiff CF14 2TL, UK
    J Immunol Methods 247:163-74. 2001
    ..The immuno-magnetic isolation and analysis of exosomes is a versatile and rapid tool for the analysis of APC exosomes, and may prove a valuable tool for the study of exosome biology...
  8. doi request reprint Resistance of CD45RA- T cells to apoptosis and functional impairment, and activation of tumor-antigen specific T cells during radiation therapy of prostate cancer
    Zsuzsanna Tabi
    Department of Pharmacology, Radiology and Oncology, School of Medicine, Cardiff University, Cancer Research Wales Research Laboratories, Velindre Cancer Centre, Whitchurch, Cardiff, CF14 2TL, UK
    J Immunol 185:1330-9. 2010
    ....
  9. doi request reprint Increased exosome production from tumour cell cultures using the Integra CELLine Culture System
    J Paul Mitchell
    Department of Oncology and Palliative Medicine, School of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, UK
    J Immunol Methods 335:98-105. 2008
    ..This tool should prove advantageous in future studies of exosome-immune modulation in cancer and other settings...
  10. doi request reprint Cancer exosomes trigger fibroblast to myofibroblast differentiation
    Jason Webber
    Department of Pharmacology, Oncology and Radiation, School of Medicine, Cardiff University, Cardiff, UK
    Cancer Res 70:9621-30. 2010
    ..This hitherto unknown trigger for instigating cellular differentiation in a distinctive manner has major implications for mechanisms underlying cancer-recruited stroma, fibrotic diseases, and wound-healing responses...
  11. pmc Can urinary exosomes act as treatment response markers in prostate cancer?
    Paul J Mitchell
    Section of Oncology and Palliative Medicine, School of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, UK
    J Transl Med 7:4. 2009
    ..Their utility as a source of markers in urological cancer remains unstudied. Our aim in this study was to investigate the feasibility and value of analysing urinary exosomes in prostate cancer patients undergoing standard therapy...
  12. pmc Proteomics analysis of bladder cancer exosomes
    Joanne L Welton
    Section of Oncology and Palliative Medicine, Department of Pharmacology, Oncology and Radiology, School of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff CF14 2TL, United Kingdom
    Mol Cell Proteomics 9:1324-38. 2010
    ..The data will aid in the development of urine exosome-based clinical tools for monitoring disease and will inform follow-up studies into varied aspects of exosome manufacture and function...
  13. doi request reprint Cancer exosomes express CD39 and CD73, which suppress T cells through adenosine production
    Aled Clayton
    Department of Pharmacology, Radiology and Oncology, School of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff CF14 2TL, United Kingdom
    J Immunol 187:676-83. 2011
    ..In summary, the data highlight exosome enzymic activity in the production of extracellular adenosine, and this may play a contributory role in negative modulation of T cells in the tumor environment...
  14. ncbi request reprint Recovery of CD8+ T-cell function during systemic chemotherapy in advanced ovarian cancer
    Sharon Coleman
    Department of Oncology and Palliative Medicine, Velindre Hospital, Whitchurch, Cardiff, UK
    Cancer Res 65:7000-6. 2005
    ..We suggest that functional assays determining T-cell immunocompetence can be valuable tools for optimizing cancer immunotherapy for improved clinical success...
  15. ncbi request reprint Anti-viral cytotoxic T cells inhibit the growth of cancer cells with antibody targeted HLA class I/peptide complexes in SCID mice
    Philip Savage
    Alexis Biotechnology, London, United Kingdom
    Int J Cancer 98:561-6. 2002
    ....
  16. doi request reprint A clinical grade poly I:C-analogue (Ampligen) promotes optimal DC maturation and Th1-type T cell responses of healthy donors and cancer patients in vitro
    H Navabi
    Velindre Cancer Centre, Velindre NHS Trust, Cardiff, UK
    Vaccine 27:107-15. 2009
    ..These characteristics make poly(I:C12U) a suitable agent for use as an adjuvant in cancer directed immunotherapeutic regimes...
  17. pmc Induction of viral and tumour specific CTL responses using antibody targeted HLA class I peptide complexes
    P Savage
    Alexis Biotechnology, 81 Harley Street, London W1N 1DE, UK
    Br J Cancer 86:1336-42. 2002
    ....
  18. ncbi request reprint The rationale for combined chemo/immunotherapy using a Toll-like receptor 3 (TLR3) agonist and tumour-derived exosomes in advanced ovarian cancer
    M Adams
    Velindre Hospital, Whitchurch, Cardiff and Cardiff University School of Medicine, Cardiff, UK
    Vaccine 23:2374-8. 2005
    ..The rationale for adopting such a combined approach is discussed here...
  19. ncbi request reprint Selective regulation of ICAM-1 and RANTES gene expression after ICAM-1 ligation on human renal fibroblasts
    Robert Blaber
    Institute of Nephrology, University of Wales College of Medicine, Heath Park, Cardiff, Wales, UK
    J Am Soc Nephrol 14:116-27. 2003
    ..This study suggests an independent mechanism of inflammatory amplification, which may be characteristic of a persistent leukocytic involvement in areas of chronic inflammation rather than in cytokine-induced acute inflammation...
  20. pmc Structural and functional changes in heparan sulfate proteoglycan expression associated with the myofibroblastic phenotype
    Gareth Thomas
    Institute of Nephrology, University of Wales College of Medicine, Heath Park, Cardiff, Wales, UK
    Am J Pathol 162:977-89. 2003
    ..A comparative investigation into HS fine structure is being undertaken to examine these findings in more detail...
  21. doi request reprint Analysis of urinary microRNAs in chronic kidney disease
    Cristina Beltrami
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Heath Park, Cardiff, UK
    Biochem Soc Trans 40:875-9. 2012
    ..Quantification of urinary microRNAs offers an alternative approach to the identification of chronic kidney disease biomarkers...
  22. ncbi request reprint Preparation of human ovarian cancer ascites-derived exosomes for a clinical trial
    H Navabi
    Cancer Services, Velindre NHS Trust, Cardiff, UK
    Blood Cells Mol Dis 35:149-52. 2005
    ....
  23. ncbi request reprint Cell surface heparan sulfate proteoglycans control the response of renal interstitial fibroblasts to fibroblast growth factor-2
    A Clayton
    Institute of Nephrology, University of Wales College of Medicine, Cardiff, Wales, United Kingdom
    Kidney Int 59:2084-94. 2001
    ....
  24. ncbi request reprint Cellular activation through the ligation of intercellular adhesion molecule-1
    A Clayton
    Institute of Nephrology, University of Wales College of Medicine, Cardiff Royal Infirmary, Cardiff CF2 1SZ, Wales, UK
    J Cell Sci 111:443-53. 1998
    ..This may have profound implications for the control and persistence of a leukocyte infiltrate and the progression of inflammatory disease...
  25. doi request reprint Isolation and characterization of exosomes from cell culture supernatants and biological fluids
    Clotilde Thery
    Institut Curie, Paris, France
    Curr Protoc Cell Biol . 2006
    ..This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosome preparations...