Robert B Clarke

Summary

Affiliation: University of Manchester
Country: UK

Publications

  1. pmc p27KIP1 phosphorylation by PKB/Akt leads to poor breast cancer prognosis
    Robert B Clarke
    Breast Biology Group, Clinical Research Department, Christie Hospital NHS Trust, Wilmslow Road, Withington, Manchester, UK
    Breast Cancer Res 5:162-3. 2003
  2. ncbi request reprint Stem cells and tissue homeostasis in mammary glands
    Robert B Clarke
    Breast Biology Group, University of Manchester, Christie Hospital, Manchester, M20 4BX UK
    J Mammary Gland Biol Neoplasia 10:1-3. 2005
  3. pmc Effects of oestrogens and anti-oestrogens on normal breast tissue from women bearing BRCA1 and BRCA2 mutations
    M Bramley
    Department of Surgery, Christie Hospital NHS Trust, Manchester M20 4BX, USA
    Br J Cancer 94:1021-8. 2006
  4. pmc Enrichment of human osteosarcoma stem cells based on hTERT transcriptional activity
    Ling Yu
    Department of Orthopedics, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, Hubei, P R China
    Oncotarget 4:2326-38. 2013
  5. pmc Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling
    Hannah Harrison
    Breast Biology Group, Institute of Cancer Sciences, University of Manchester, Paterson Institute for Cancer Research, Manchester, M20 4BX, UK
    Breast Cancer Res 15:R21. 2013
  6. pmc The devil is in the methods: lineage tracing, functional screens and sequencing, hormones, tumour-stroma interactions, and expansion of human breast tumours as xenografts
    María del Mar Vivanco
    CIC bioGUNECell Biology and Stem Cells UnitParque Tecnológico de Bizkaia, Ed 801A48160 Derio Bizkaia, Spain
    Breast Cancer Res 13:316. 2011
  7. pmc It's all in the details: methods in breast development and cancer
    Mohamed Bentires-Alj
    Friedrich Miescher Institute for Biomedical Research FMI, Maulbeerstr, 66 CH 4058 Basel, Switzerland
    Breast Cancer Res 11:305. 2009
  8. pmc Disruption of a Quorum Sensing mechanism triggers tumorigenesis: a simple discrete model corroborated by experiments in mammary cancer stem cells
    Zvia Agur
    Institute for Medical Biomathematics, 10 Hate ena St, Bene Ataroth, Israel
    Biol Direct 5:20. 2010
  9. ncbi request reprint Ovarian steroids and the human breast: regulation of stem cells and cell proliferation
    Robert B Clarke
    Breast Biology Group, CR UK Department of Medical Oncology, University of Manchester, Christie Hospital, Manchester, M20 4BX, UK
    Maturitas 54:327-34. 2006
  10. ncbi request reprint Steroid receptors and proliferation in the human breast
    Robert B Clarke
    Breast Biology Group, Clinical Research Department, Christie Hospital, Manchester M20 4BX, UK
    Steroids 68:789-94. 2003

Collaborators

Detail Information

Publications46

  1. pmc p27KIP1 phosphorylation by PKB/Akt leads to poor breast cancer prognosis
    Robert B Clarke
    Breast Biology Group, Clinical Research Department, Christie Hospital NHS Trust, Wilmslow Road, Withington, Manchester, UK
    Breast Cancer Res 5:162-3. 2003
  2. ncbi request reprint Stem cells and tissue homeostasis in mammary glands
    Robert B Clarke
    Breast Biology Group, University of Manchester, Christie Hospital, Manchester, M20 4BX UK
    J Mammary Gland Biol Neoplasia 10:1-3. 2005
  3. pmc Effects of oestrogens and anti-oestrogens on normal breast tissue from women bearing BRCA1 and BRCA2 mutations
    M Bramley
    Department of Surgery, Christie Hospital NHS Trust, Manchester M20 4BX, USA
    Br J Cancer 94:1021-8. 2006
    ..We conclude that early anti-oestrogen treatment should prevent breast cancer in very high-risk women...
  4. pmc Enrichment of human osteosarcoma stem cells based on hTERT transcriptional activity
    Ling Yu
    Department of Orthopedics, Renmin Hospital of Wuhan University, Jiefang Road, Wuhan, Hubei, P R China
    Oncotarget 4:2326-38. 2013
    ..These data support telomerase inhibition as a potential targeted therapy for osteosarcoma stem-like cells. ..
  5. pmc Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling
    Hannah Harrison
    Breast Biology Group, Institute of Cancer Sciences, University of Manchester, Paterson Institute for Cancer Research, Manchester, M20 4BX, UK
    Breast Cancer Res 15:R21. 2013
    ..CONCLUSIONS: These data establish that EGF and Notch receptor signalling pathways operate downstream of oestrogen in the regulation of ER negative CSCs...
  6. pmc The devil is in the methods: lineage tracing, functional screens and sequencing, hormones, tumour-stroma interactions, and expansion of human breast tumours as xenografts
    María del Mar Vivanco
    CIC bioGUNECell Biology and Stem Cells UnitParque Tecnológico de Bizkaia, Ed 801A48160 Derio Bizkaia, Spain
    Breast Cancer Res 13:316. 2011
    ..The third meeting in this series, held in April-May 2011 in Weggis, Switzerland, focussed on functional screens and sequencing, hormones, lineage tracing, tumor-stroma interactions and the expansion of human breast tumours as xenografts...
  7. pmc It's all in the details: methods in breast development and cancer
    Mohamed Bentires-Alj
    Friedrich Miescher Institute for Biomedical Research FMI, Maulbeerstr, 66 CH 4058 Basel, Switzerland
    Breast Cancer Res 11:305. 2009
    ....
  8. pmc Disruption of a Quorum Sensing mechanism triggers tumorigenesis: a simple discrete model corroborated by experiments in mammary cancer stem cells
    Zvia Agur
    Institute for Medical Biomathematics, 10 Hate ena St, Bene Ataroth, Israel
    Biol Direct 5:20. 2010
    ..Theory, predicting that homeostasis is maintained by a negative feedback on stem cell proliferation, implies a Quorum Sensing mechanism in higher vertebrates...
  9. ncbi request reprint Ovarian steroids and the human breast: regulation of stem cells and cell proliferation
    Robert B Clarke
    Breast Biology Group, CR UK Department of Medical Oncology, University of Manchester, Christie Hospital, Manchester, M20 4BX, UK
    Maturitas 54:327-34. 2006
    ....
  10. ncbi request reprint Steroid receptors and proliferation in the human breast
    Robert B Clarke
    Breast Biology Group, Clinical Research Department, Christie Hospital, Manchester M20 4BX, UK
    Steroids 68:789-94. 2003
    ..Such hormone sensor cells might secrete positive or negative paracrine/juxtacrine factors dependent on the prevailing E or P concentration to influence the proliferative activity of adjacent ERalpha/PR-negative epithelial cells...
  11. ncbi request reprint A putative human breast stem cell population is enriched for steroid receptor-positive cells
    Robert B Clarke
    Breast Biology Group, Cancer Research UK Department of Medical Oncology, University of Manchester, Christie Hospital NHS Trust, Wilmslow Road, Withington, Manchester M20 4BX, UK
    Dev Biol 277:443-56. 2005
    ..The data suggest a model where scattered steroid receptor-positive cells are stem cells that self-renew through asymmetric cell division and generate patches of transit amplifying and differentiated cells...
  12. ncbi request reprint Steroid receptors in human breast cancer
    Robert B Clarke
    CR UK Department of Medical Oncology, Christie Hospital, Manchester, UK
    Trends Endocrinol Metab 15:316-23. 2004
    ..These interactions might explain resistance to endocrine treatments and offer solutions in terms of novel therapeutic targets...
  13. ncbi request reprint The oestrogen receptor (ER) in normal and abnormal uterine tissue
    K J Neis
    Department of Obstetrics and Gynecology Caritasklinik St Theresia Saarbrücken, Academic Hospital Universität des Saarlandes Rheinstrasse 2, D 66113, Saarbrucken, Germany
    Eur J Cancer 36:S30-1. 2000
    ..The ER only disappears when nuclear irregularities occur in cases of adenomatous hyperplasia. In cases of invasive carcinoma, a heterogenous picture is seen which closely correlates with the degree of differentiation...
  14. ncbi request reprint Regulation of human breast epithelial stem cells
    Robert B Clarke
    Breast Biology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Withington, Manchester, UK
    Cell Prolif 36:45-58. 2003
    ..ERalpha/PR+ breast cancers exhibit loss of the two key regulators of asymmetric cell division, Musashi-1 and Notch-1 and thus may arise from symmetric division of the ERalpha/PR+ stem cell...
  15. ncbi request reprint Contrasting hypoxic effects on breast cancer stem cell hierarchy is dependent on ER-α status
    Hannah Harrison
    Molecular Pathology, Breakthrough Breast Cancer, School of Cancer, Paterson Institute for Cancer Research, University of Manchester, UK
    Cancer Res 73:1420-33. 2013
    ..These data suggest that ER-α-positive and negative breast cancer subtypes respond differently to hypoxia and, as a consequence, antiangiogenic therapies will not be suitable for both subgroups...
  16. pmc Regulation of breast cancer stem cell activity by signaling through the Notch4 receptor
    Hannah Harrison
    Breast Biology Group, School of Cancer, Enabling Sciences and Technology, Paterson Institute for Cancer Research, University of Manchester, Manchester Academic Health Sciences Centre, The Christie NHS Foundation Trust Department of Medical Oncology, the Christie NHS Foundation Trust, Manchester M20 4BX, United Kingdom
    Cancer Res 70:709-18. 2010
    ..Our findings suggest that Notch4-targeted therapies will be more effective than targeting Notch1 in suppressing breast cancer recurrence, as it is initiated by breast cancer stem cells...
  17. ncbi request reprint Normal breast tissue implanted into athymic nude mice identifies biomarkers of the effects of human pregnancy levels of estrogen
    Rognvald N Blance
    Breast Biology Group, School of Cancer and Imaging Sciences, Paterson Institute for Cancer Research, University of Manchester, Manchester, United Kingdom
    Cancer Prev Res (Phila) 2:257-64. 2009
    ....
  18. pmc Wnt pathway activity in breast cancer sub-types and stem-like cells
    Rebecca Lamb
    Breakthrough Breast Cancer Research Unit, University of Manchester, Manchester, England
    PLoS ONE 8:e67811. 2013
    ..Wnt signalling has been implicated in stem cell regulation however its role in breast cancer stem cell regulation remains unclear...
  19. doi request reprint A detailed mammosphere assay protocol for the quantification of breast stem cell activity
    Frances L Shaw
    Breast Biology Group, School of Cancer and enabling Sciences, Paterson Institute for Cancer Research, The University of Manchester, Wilmslow Road, Manchester, UK
    J Mammary Gland Biol Neoplasia 17:111-7. 2012
    ..We describe here a detailed mammosphere assay protocol for the assessment of stem cell activity and self-renewal, and discuss how data generated by the assay can be analysed and interpreted...
  20. doi request reprint Breast cancer stem cells: something out of notching?
    Hannah Harrison
    Breast Biology Group, School of Cancer and enabling Sciences, Paterson Institute for Cancer Research, University of Manchester, Manchester, United Kingdom
    Cancer Res 70:8973-6. 2010
    ....
  21. ncbi request reprint Steroid receptors and cell cycle in normal mammary epithelium
    Elizabeth Anderson
    Christie Hospital NHS Trust, Manchester, UK
    J Mammary Gland Biol Neoplasia 9:3-13. 2004
    ..Answering these questions could greatly increase our understanding of the factors controlling mammary gland development and the processes leading to cancer formation...
  22. ncbi request reprint Estrogen deprivation for breast cancer prevention
    Anthony Howell
    CRUK Department of Medical Oncology, Christie Hospital, University of Manchester, UK
    Recent Results Cancer Res 174:151-67. 2007
    ..It may be possible to increase effectiveness of ED by additional preventive agents or by lifestyle alterations...
  23. ncbi request reprint Origins of breast cancer subtypes and therapeutic implications
    Andrew H Sims
    Breast Biology Group, Paterson Institute for Cancer Research, University of Manchester, Manchester, UK
    Nat Clin Pract Oncol 4:516-25. 2007
    ..Consequently, inhibition of stem-cell self-renewal pathways should be explored because of the likelihood that residual stem cells might be resistant to current therapies...
  24. doi request reprint Targeting CXCR1/2 significantly reduces breast cancer stem cell activity and increases the efficacy of inhibiting HER2 via HER2-dependent and -independent mechanisms
    Jagdeep K Singh
    Breast Biology Group, Institute of Cancer Sciences, Paterson Institute for Cancer Research, University of Manchester, Manchester, United Kingdom
    Clin Cancer Res 19:643-56. 2013
    ..We investigated the role of IL-8 in the regulation of CSC activity using patient-derived breast cancers and determined the potential benefit of combining CXCR1/2 inhibition with HER2-targeted therapy...
  25. pmc Prolactin receptor antagonism reduces the clonogenic capacity of breast cancer cells and potentiates doxorubicin and paclitaxel cytotoxicity
    Sacha J Howell
    Breast Biology Group, Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester M20 4BX, UK
    Breast Cancer Res 10:R68. 2008
    ..We examined the effects of the 'pure' prolactin receptor antagonist Delta1-9-G129R-hPrl (Delta1-9) on the breast cancer cell number and clonogenicity, alone and in combination with chemotherapy...
  26. ncbi request reprint Human breast epithelial stem cells and their regulation
    Helen Kalirai
    Breast Biology Group, Division of Cancer Studies, University of Manchester, Christie Hospital, Wilmslow Road, Manchester, M20 4BX, UK
    J Pathol 208:7-16. 2006
    ..By understanding the molecular pathways that regulate self-renewal of normal mammary stem cells, it may be possible to target the activation of these pathways in breast tumours...
  27. pmc Targeting inhibitor of apoptosis proteins in combination with ErbB antagonists in breast cancer
    Fiona M Foster
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    Breast Cancer Res 11:R41. 2009
    ..Here we examine the levels of IAPs in breast cancer and evaluate whether targeting IAPs can enhance apoptosis in response to growth factor receptor antagonists and TRAIL...
  28. ncbi request reprint Biomarkers of dietary energy restriction in women at increased risk of breast cancer
    Kai Ren Ong
    Breast Biology Group, School of Cancer and Imaging Sciences, Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester M20 4BX, United Kingdom
    Cancer Prev Res (Phila) 2:720-31. 2009
    ..We conclude that reduced expressions of genes in the lipid metabolism and glycolytic pathways are detectable in breast tissue following DER, and these may represent targets for DER mimetics as effective chemoprophylactic agents...
  29. pmc Effect of a farnesyl transferase inhibitor (R115777) on ductal carcinoma in situ of the breast in a human xenograft model and on breast and ovarian cancer cell growth in vitro and in vivo
    Fredrik Warnberg
    Breast Biology Group, Christie Hospital NHS Trust, Manchester, UK
    Breast Cancer Res 8:R21. 2006
    ..Effects on tumour xenografts and human ductal carcinoma in situ (DCIS) of the breast in a xenograft mouse model were also tested...
  30. ncbi request reprint Mammary stem cells and breast cancer--role of Notch signalling
    Gillian Farnie
    Breast Biology Group, Division of Cancer Studies, Faculty of Medicine and Human Sciences, University of Manchester, Paterson Institute for Cancer Research, Wilmslow Road, Manchester, M20 4BX, UK
    Stem Cell Rev 3:169-75. 2007
    ....
  31. ncbi request reprint Targeting IL-8 signalling to inhibit breast cancer stem cell activity
    Jagdeep K Singh
    University of Manchester, Institute of Cancer Sciences, Paterson Institute for Cancer Research, Breast Biology Group, Wilmslow Road, Manchester, M20 4BX, UK 44 161 446 3210
    Expert Opin Ther Targets 17:1235-41. 2013
    ..Here, we discuss the role of IL-8 in breast CSC regulation and development of novel therapies to target CXCR1/2 signalling in breast cancer. ..
  32. ncbi request reprint Novel cell culture technique for primary ductal carcinoma in situ: role of Notch and epidermal growth factor receptor signaling pathways
    Gillian Farnie
    Department of Surgery and Breast Biology Group, Division of Cancer Studies, Faculty of Medicine and Human Sciences, University of Manchester, Christie Hospital NHS Trust, Wilmslow Road, M20 9BX, Manchester, UK
    J Natl Cancer Inst 99:616-27. 2007
    ..We investigated the involvement of these signaling pathways in ductal carcinoma in situ (DCIS) of the breast...
  33. doi request reprint Resistance to endocrine therapy: are breast cancer stem cells the culprits?
    Ciara S O'Brien
    Breast Biology Group, School of Cancer and Imaging Sciences, Paterson Institute for Cancer Research, University of Manchester, Manchester, UK
    J Mammary Gland Biol Neoplasia 14:45-54. 2009
    ....
  34. doi request reprint Breast cancer stem cells and their role in resistance to endocrine therapy
    Ciara S O'Brien
    School of Cancer and Imaging Sciences, Paterson Institute for Cancer Research, University of Manchester, Manchester, M20 4BX, UK
    Horm Cancer 2:91-103. 2011
    ....
  35. pmc A differential role for CXCR4 in the regulation of normal versus malignant breast stem cell activity
    Matthew P Ablett
    Breast Biology Group, Institute of Cancer Sciences, Paterson Building, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK
    Oncotarget 5:599-612. 2014
    ..Here, we demonstrate that CXCR4 signalling specifically regulates breast cancer stem cell activities and may therefore be important in tumour formation at the sites of metastases. ..
  36. pmc Combined inhibition of ErbB1/2 and Notch receptors effectively targets breast ductal carcinoma in situ (DCIS) stem/progenitor cell activity regardless of ErbB2 status
    Gillian Farnie
    Cancer Stem Cell Research, University of Manchester, Manchester Academic Health Science Centre, Paterson Institute for Cancer Research, the Christie NHS Foundation Trust, Manchester, United Kingdom
    PLoS ONE 8:e56840. 2013
    ..These data provide further understanding of DCIS biology and suggest treatment strategies combining Notch and ErbB1/2 inhibitors should be investigated regardless of ErbB2 receptor status...
  37. doi request reprint Stem cells in breast tumours: are they ready for the clinic?
    Matthew P Ablett
    Breast Biology Group, School of Cancer and enabling Sciences, University of Manchester, Wilmslow Road, Manchester M20 4BX, UK
    Eur J Cancer 48:2104-16. 2012
    ..This is likely to be demanding but feasible in the near future. Thus, we asked if CSCs are ready for the clinic, however, the emerging question becomes: is the clinic ready for cancer stem cells?..
  38. pmc High-throughput genomic technology in research and clinical management of breast cancer. Exploiting the potential of gene expression profiling: is it ready for the clinic?
    Andrew H Sims
    Breast Biology Group, University of Manchester, Paterson Institute for Cancer Research, Wilmslow Road, Manchester, M20 4BX, UK
    Breast Cancer Res 8:214. 2006
    ..Gene expression profiling is beginning to appear in the breast cancer clinic but it is not yet fully evaluated. This review explores the questions that must be addressed before this technology can become an everyday clinical tool...
  39. doi request reprint The origin of estrogen receptor alpha-positive and alpha-negative breast cancer
    Robert B Clarke
    Breast Biology Group, Division of Cancer Studies, University of Manchester Christie Hospital NHS Trust, Manchester, UK
    Adv Exp Med Biol 617:79-86. 2008
  40. ncbi request reprint Mechanisms of Disease: prediction and prevention of breast cancer--cellular and molecular interactions
    Anthony Howell
    Dept of Medical Oncology, University of Manchester, Christie Hospital, Withington, UK
    Nat Clin Pract Oncol 2:635-46. 2005
    ..New approaches are required, however, not only to prevent the disease but to devise methods for their assessment that do not require very large and expensive clinical trials...
  41. ncbi request reprint 17β-estradiol regulates giant vesicle formation via estrogen receptor-alpha in human breast cancer cells
    Paul K Wright
    Department of Histopathology, Manchester Royal Infirmary, Central Manchester University Hospitals NHS Foundation Trust, Manchester, UK
    Oncotarget 5:3055-65. 2014
    ....
  42. pmc Do early premalignant changes in normal breast epithelial cells predict cancer development?
    Robert B Clarke
    CR UK Department of Medical Oncology, University of Manchester, Christie Hospital NHS Trust, Manchester, UK
    Breast Cancer Res 7:18-20. 2005
    ....
  43. pmc Combining Notch inhibition with current therapies for breast cancer treatment
    Keith Brennan
    Wellcome Trust Centre for Cell Matrix Research and Manchester Breast Centre, Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Ther Adv Med Oncol 5:17-24. 2013
    ..In particular, recent studies suggest that the greatest success will come from combining Notch pathway inhibitors with current breast cancer therapies...
  44. ncbi request reprint The centrosomal kinase Nek2 displays elevated levels of protein expression in human breast cancer
    Daniel G Hayward
    Department of Biochemistry, University of Leicester, Leicester, USA
    Cancer Res 64:7370-6. 2004
    ..These data highlight the Nek2 kinase as novel potential target for chemotherapeutic intervention in breast cancer...
  45. ncbi request reprint The mammary gland "side population": a putative stem/progenitor cell marker?
    Matthew J Smalley
    The Breakthrough Breast Cancer Centre, The Institute of Cancer Research, London, United Kingdom
    J Mammary Gland Biol Neoplasia 10:37-47. 2005
    ..However, evidence that the SP is enriched for stem cells is, at the moment, only correlative, and there are potentially confounding technical issues. We still await formal proof that the SP contains a stem cell population...