Research Topics
| M ChristodoulidesSummaryAffiliation: University of Southampton Country: UK Publications
| Collaborators
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Detail Information
Publications
Immunization with recombinant class 1 outer-membrane protein from Neisseria meningitidis: influence of liposomes and adjuvants on antibody avidity, recognition of native protein and the induction of a bactericidal immune response against meningococciM Christodoulides
Molecular Microbiology Group, University of Southampton Medical School, Southampton General Hospital, UK
Microbiology 144:3027-37. 1998..These data show that liposomes containing recombinant class 1 protein represent a potential basis of future vaccines, of defined composition, designed for the prevention of group B meningococcal infections...- Effect of adjuvant composition on immune response to a multiple antigen peptide (MAP) containing a protective epitope from Neisseria meningitidis class 1 porinM Christodoulides
Molecular Microbiology Group, University of Southampton Medical School, Southampton General Hospital, UK
Vaccine 18:131-9. 1999.... - Interaction of primary human endometrial cells with Neisseria gonorrhoeae expressing green fluorescent proteinM Christodoulides
Molecular Microbiology Group, University of Southampton Medical School, UK
Mol Microbiol 35:32-43. 2000.... 
Interaction of Neisseria meningitidis with human meningeal cells induces the secretion of a distinct group of chemotactic, proinflammatory, and growth-factor cytokinesMyron Christodoulides
Molecular Microbiology and Infection, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, United Kingdom
Infect Immun 70:4035-44. 2002....- Immunization with recombinant Opc outer membrane protein from Neisseria meningitidis: influence of sequence variation and levels of expression on the bactericidal immune response against meningococciK A Jolley
Molecular Microbiology Group, Division of Cell and Molecular Medicine, University of Southampton Medical School, Southampton General Hospital, United Kingdom
Infect Immun 69:3809-16. 2001..The magnitude of the bactericidal effect was strongly influenced by the level of expression of the Opc protein and was abolished by limited variation in the sequence of the protein expressed by heterologous strains... - Immunization with the recombinant PorB outer membrane protein induces a bactericidal immune response against Neisseria meningitidisJ Claire Wright
Molecular Microbiology and Infection, Division of Infection, Inflammation and Repair, University of Southampton Medical School, and Southampton General Hospital, United Kingdom
Infect Immun 70:4028-34. 2002..These results demonstrate that the PorB protein should be considered as a component of a vaccine designed to prevent serogroup B meningococcal infection... - Dynamics of carriage of Neisseria meningitidis in a group of military recruits: subtype stability and specificity of the immune response following colonizationG R Jones
Molecular Microbiology Group, University of Southampton Medical School, Southampton General Hospital, United Kingdom
J Infect Dis 178:451-9. 1998..The subtype specificity of the bactericidal response to meningococci and the stability of expression of the class 1 protein have important implications for the design of vaccines for prevention of serogroup B meningococcal disease... - Activation of human meningeal cells is modulated by lipopolysaccharide (LPS) and non-LPS components of Neisseria meningitidis and is independent of Toll-like receptor (TLR)4 and TLR2 signallingHolly E Humphries
Molecular Microbiology, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, UK
Cell Microbiol 7:415-30. 2005..Moreover, the biological consequences of cellular activation by non-LPS modulins suggest that clinical intervention strategies based solely on abrogating the effects of LPS are likely to be only partially effective... - Interactions of Neisseria gonorrhoeae with mature human macrophage opacity proteins influence production of proinflammatory cytokinesB L Makepeace
Molecular Microbiology Group, Division of Cell and Molecular Medicine, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom
Infect Immun 69:1909-13. 2001..Thus, variation in Opa protein expression by the gonococcus may be a determining factor in the severity of pelvic inflammatory disease... - Multivalent liposome-based vaccines containing different serosubtypes of PorA protein induce cross-protective bactericidal immune responses against Neisseria meningitidisHolly E Humphries
Molecular Microbiology Group, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Mailpoint 814, Level C, Lab and Path Block, Tremona Road, Southampton SO16 6YD, UK
Vaccine 24:36-44. 2006..These data demonstrate that multivalent liposome vaccines, containing multiple PorA serosubtypes, have the potential to provide protection against a broad range of meningococcal strains... - Activation of human dendritic cells by the PorA protein of Neisseria meningitidisTamara Al-Bader
Dermatopharmacology Unit, Division of Infection, Inflammation and Repair, Southampton General Hospital, Southampton SO16 6YD, UK
Cell Microbiol 6:651-62. 2004.... - Different meningitis-causing bacteria induce distinct inflammatory responses on interaction with cells of the human meningesMark I Fowler
Molecular Microbiology and Infection, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, UK
Cell Microbiol 6:555-67. 2004.... - Recombinant meningococcal PorA protein, expressed using a vector system with potential for human vaccination, induces a bactericidal immune responseHolly E Humphries
Molecular Microbiology and Infection, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK
Vaccine 22:1564-9. 2004.... - Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tagHolly E Humphries
Molecular Microbiology and Infection, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Tremona Road, SO16 6YD, Southampton, UK
Protein Expr Purif 26:243-8. 2002..Thus, the IMPACT-TWIN system provides a facile method for producing recombinant PorA and may also be useful for the production of other bacterial outer-membrane proteins for vaccine studies... - Lack of immunity in university students before an outbreak of serogroup C meningococcal infectionG R Jones
Public Health Laboratory, Southampton General Hospital, Southampton SO16 6YD, United Kingdom
J Infect Dis 181:1172-5. 2000..A low prevalence of carriage of the outbreak strain, together with a low prevalence of protective immunity within the student population, was associated with a high incidence of invasive disease in those who acquired the outbreak strain... - Activation of human dendritic cells is modulated by components of the outer membranes of Neisseria meningitidisTamara Al-Bader
Dermatopharmacology Unit, Southampton General Hospital, Southampton SO16 6YD, United Kingdom
Infect Immun 71:5590-7. 2003..These data demonstrate that components of the OM, other than LPS, are also likely to be involved in determining the levels of DC activation and the nature of the T-helper immune response... - Comparison of the inflammatory responses of human meningeal cells following challenge with Neisseria lactamica and with Neisseria meningitidisMark I Fowler
Molecular Microbiology Group, Division of Infection, Inflammation and Repair, Mailpoint 814, University of Southampton Medical School, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom
Infect Immun 74:6467-78. 2006..It is likely that differential expression of modulins between N. lactamica and N. meningitidis contributes to these observed differences in pathogenic potential... - A DNA fusion vaccine induces bactericidal antibodies to a peptide epitope from the PorA porin of Neisseria meningitidisDelin Zhu
Genetic Vaccine Group, Cancer Sciences Division, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom
Infect Immun 76:334-8. 2008..These data provide proof of principle for a DNA fusion plasmid strategy as a novel approach to preparing vaccines based on defined, protective epitopes... - Immunoproteomic analysis of the development of natural immunity in subjects colonized by Neisseria meningitidis reveals potential vaccine candidatesJeannette N Williams
Molecular Microbiology Group, Division of Infection, Inflammation and Immunity, University of Southampton Medical School, Mailpoint 814, Southampton General Hospital, Southampton SO16 6YD, United Kingdom
Infect Immun 77:5080-9. 2009..The list of protein immunogens generated included not only well-established antigens but also novel proteins that represent potentially new candidates for inclusion in defined, multicomponent serogroup B vaccines... - A cyanobacterial lipopolysaccharide antagonist inhibits cytokine production induced by Neisseria meningitidis in a human whole-blood model of septicemiaKim Jemmett
Neisseria Research Group, Molecular Microbiology, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom
Infect Immun 76:3156-63. 2008..These data demonstrate that CyP is a potent antagonist of meningococcal LPS and could be considered a new adjunctive therapy for treating septicemia... 
Identification of novel pneumolysin alleles from paediatric carriage isolates of Streptococcus pneumoniaeJ M C Jefferies
Molecular Microbiology and Infection, Division of Infection, Inflammation and Immunity, University of Southampton School of Medicine, Southampton General Hospital, Southampton SO16 6YD, UK
J Med Microbiol 59:808-14. 2010....- Neuropeptide alpha-MSH exerts pro-inflammatory effects on Neisseria meningitidis infection in vitroPawan Kumar
Division of Infection, Inflammation and Immunity, Department of Dermatopharmacology, University of Southampton Medical School, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK
Inflamm Res 59:105-13. 2010..We investigated the therapeutic effects of alpha-MSH administration on Neisseria meningitidis infection of human meningeal cell cultures in vitro... - Proteomic analysis of outer membranes and vesicles from wild-type serogroup B Neisseria meningitidis and a lipopolysaccharide-deficient mutantJeannette N Williams
Molecular Microbiology Group, Division of Infection Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom
Infect Immun 75:1364-72. 2007..These results have important implications for the development and use of vaccines based on outer membrane proteins... - Development of immunity to serogroup B meningococci during carriage of Neisseria meningitidis in a cohort of university studentsJ Zoe Jordens
Molecular Microbiology and Infection Group, University of Southampton Medical School, UK
Infect Immun 72:6503-10. 2004.... - The Neisseria meningitidis macrophage infectivity potentiator protein induces cross-strain serum bactericidal activity and is a potential serogroup B vaccine candidateMiao Chiu Hung
Neisseria Research Laboratory, Molecular Microbiology, Division of Infection, Inflammation and Immunity, Sir Henry Wellcome Research Laboratories, MP814, University of Southampton Medical School, Southampton SO16 6YD, United Kingdom
Infect Immun 79:3784-91. 2011..Taken together, these findings suggest that rMIP can provide cross-strain protection against meningococci and should be considered a potential antigen for inclusion in new vaccines against meningococcal infection... - Serological correlates of protection against meningococci in a cohort of university students, before and during an outbreak of serogroup C infectionJeannette N Williams
Molecular Microbiology and Infection Group, University of Southampton Medical School, Southampton, United Kingdom
J Infect Dis 187:1433-41. 2003.... - Expression of proinflammatory cytokines and receptors by human fallopian tubes in organ culture following challenge with Neisseria gonorrhoeaeKevin Maisey
Laboratorio de Inmunología de la Reproducción, Facultad de Quimica y Biologia, Universidad de Santiago de Chile USACH Instituto Chileno de Medicina Reproductiva, Santiago, Chile
Infect Immun 71:527-32. 2003..These studies suggest that several proinflammatory cytokines are likely to contribute to the cell and tissue damage observed in gonococcal salpingitis...