Ian Chambers

Summary

Affiliation: University of Edinburgh
Country: UK

Publications

  1. pmc The transcriptional foundation of pluripotency
    Ian Chambers
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, Edinburgh EH9 3JQ, UK
    Development 136:2311-22. 2009
  2. pmc Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells
    Hitoshi Niwa
    Stem Cell Regulation Research, Area of Molecular Therapeutics, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Suita C, Osaka 565 0871, Japan
    Mol Cell Biol 22:1526-36. 2002
  3. ncbi request reprint Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
  4. ncbi request reprint BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, EH9 3JQ, Edinburgh, Scotland
    Cell 115:281-92. 2003
  5. ncbi request reprint Self-renewal of teratocarcinoma and embryonic stem cells
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Rd, EH9 3JQ, Scotland, UK
    Oncogene 23:7150-60. 2004
  6. pmc Esrrb is a direct Nanog target gene that can substitute for Nanog function in pluripotent cells
    Nicola Festuccia
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, Scotland
    Cell Stem Cell 11:477-90. 2012
  7. pmc Reduced Oct4 expression directs a robust pluripotent state with distinct signaling activity and increased enhancer occupancy by Oct4 and Nanog
    Violetta Karwacki-Neisius
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, Scotland, UK
    Cell Stem Cell 12:531-45. 2013
  8. ncbi request reprint Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
  9. ncbi request reprint The molecular basis of pluripotency in mouse embryonic stem cells
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Rd, Edinburgh EH9 3JQ, Scotland
    Cloning Stem Cells 6:386-91. 2004
  10. pmc OCT4/SOX2-independent Nanog autorepression modulates heterogeneous Nanog gene expression in mouse ES cells
    Pablo Navarro
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland
    EMBO J 31:4547-62. 2012

Collaborators

Detail Information

Publications28

  1. pmc The transcriptional foundation of pluripotency
    Ian Chambers
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, Edinburgh EH9 3JQ, UK
    Development 136:2311-22. 2009
    ..Here we discuss how these observations advance our understanding of the transcription factor network that controls pluripotent identity and highlight unresolved issues that arise from these studies...
  2. pmc Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells
    Hitoshi Niwa
    Stem Cell Regulation Research, Area of Molecular Therapeutics, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Suita C, Osaka 565 0871, Japan
    Mol Cell Biol 22:1526-36. 2002
    ..Interestingly, however, Oct-3/4 target gene expression elicited by the N- and C-terminal transactivation domains is not identical, indicating that at least one class of genes activated by Oct-3/4 is not required for ES cell propagation...
  3. ncbi request reprint Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
    ..These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity...
  4. ncbi request reprint BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3
    Qi Long Ying
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, EH9 3JQ, Edinburgh, Scotland
    Cell 115:281-92. 2003
    ..Upon LIF withdrawal, Id-expressing ES cells differentiate but do not give rise to neural lineages. We conclude that blockade of lineage-specific transcription factors by Id proteins enables the self-renewal response to LIF/STAT3...
  5. ncbi request reprint Self-renewal of teratocarcinoma and embryonic stem cells
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Rd, EH9 3JQ, Scotland, UK
    Oncogene 23:7150-60. 2004
    ..Nanog function also requires Oct4. Here, we review recent progress in ES cell self-renewal, relate this to the biology of teratocarcinomas and offer testable hypotheses to expose the mechanics of ES cell self-renewal...
  6. pmc Esrrb is a direct Nanog target gene that can substitute for Nanog function in pluripotent cells
    Nicola Festuccia
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, Scotland
    Cell Stem Cell 11:477-90. 2012
    ..Finally, Esrrb deletion abolishes the defining ability of Nanog to confer LIF-independent ESC self-renewal. These findings are consistent with the functional placement of Esrrb downstream of Nanog...
  7. pmc Reduced Oct4 expression directs a robust pluripotent state with distinct signaling activity and increased enhancer occupancy by Oct4 and Nanog
    Violetta Karwacki-Neisius
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, Scotland, UK
    Cell Stem Cell 12:531-45. 2013
    ..Our findings suggest that cells with a reduced Oct4 concentration range are maintained in a robust pluripotent state and that the wild-type Oct4 concentration range enables effective differentiation...
  8. ncbi request reprint Nanog safeguards pluripotency and mediates germline development
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 450:1230-4. 2007
    ..We surmise that Nanog stabilizes embryonic stem cells in culture by resisting or reversing alternative gene expression states...
  9. ncbi request reprint The molecular basis of pluripotency in mouse embryonic stem cells
    Ian Chambers
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Rd, Edinburgh EH9 3JQ, Scotland
    Cloning Stem Cells 6:386-91. 2004
    ..These observations are brought together to provide a genetic model of ES cell self-renewal centered upon interactions between Oct4, STAT3 and Nanog...
  10. pmc OCT4/SOX2-independent Nanog autorepression modulates heterogeneous Nanog gene expression in mouse ES cells
    Pablo Navarro
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland
    EMBO J 31:4547-62. 2012
    ..Therefore, cellular variability in self-renewal efficiency is an emergent property of the pluripotency gene regulatory network...
  11. ncbi request reprint Nanog promotes transfer of pluripotency after cell fusion
    Jose Silva
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, EH9 3JQ, UK
    Nature 441:997-1001. 2006
    ..We conclude that Nanog can orchestrate ES cell machinery to instate pluripotency with an efficiency of up to 100% depending on the differentiation status of the somatic cell...
  12. doi request reprint The pluripotency rheostat Nanog functions as a dimer
    Nicholas P Mullin
    MRC Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, U K
    Biochem J 411:227-31. 2008
    ....
  13. ncbi request reprint Nanog retrotransposed genes with functionally conserved open reading frames
    Morag Robertson
    Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh, EH9 3JQ, Scotland
    Mamm Genome 17:732-43. 2006
    ..caroli and M. spretus and indicates that Nanog retrotransposition events continue to occur at a high frequency, a property likely to extend to other germ-line transcripts...
  14. pmc The developmental dismantling of pluripotency is reversed by ectopic Oct4 expression
    Rodrigo Osorno
    Institute for Stem Cell Research, MRC Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK
    Development 139:2288-98. 2012
    ..Our data suggest that decreasing Oct4 expression is converted to a sudden drop in competence to maintain pluripotency gene regulatory network activity that is subsequently stabilized by epigenetic locks...
  15. doi request reprint Quantification of pluripotency transcription factor levels in embryonic stem cells by flow cytometry
    Nicola Festuccia
    Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, United Kingdom
    Curr Protoc Stem Cell Biol . 2011
    ..We illustrate the application of this technique to the detection of Oct4 and Nanog proteins and the coupling of this approach with fluorescent reporters of gene activity...
  16. pmc The role of pluripotency gene regulatory network components in mediating transitions between pluripotent cell states
    Nicola Festuccia
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, Scotland, United Kingdom Electronic address
    Curr Opin Genet Dev 23:504-11. 2013
    ..g. Nanog) or eliminated (e.g. Esrrb). Re-expressing such TFs can move cells back to an earlier developmental identity and is being applied to attempt establishment of human cell lines with the properties of mouse ES cells. ..
  17. pmc A direct physical interaction between Nanog and Sox2 regulates embryonic stem cell self-renewal
    Alessia Gagliardi
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
    EMBO J 32:2231-47. 2013
    ..These results suggest that aromatic stacking of Nanog tryptophans and Sox2 tyrosines mediates an interaction central to ES cell self-renewal. ..
  18. pmc Hes1 desynchronizes differentiation of pluripotent cells by modulating STAT3 activity
    Xinzhi Zhou
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
    Stem Cells 31:1511-22. 2013
    ..Variability in Hes1 expression therefore helps to explain why STAT3 responsiveness varies between individual ES cells, and this in turn helps to explain why pluripotent cells commit to differentiate asynchronously...
  19. pmc The X-inactivation trans-activator Rnf12 is negatively regulated by pluripotency factors in embryonic stem cells
    Pablo Navarro
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland, UK
    Hum Genet 130:255-64. 2011
    ..We conclude that in mouse ES cells the pluripotency-associated machinery exerts an exhaustive control of X-inactivation by taking over the regulation of all three major regulators of X-inactivation: Xist, Tsix, and Rnf12...
  20. ncbi request reprint Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation
    Jerome Aubert
    Institute for Stem Cell Research, University of Edinburgh, The King s Buildings, West Mains Road, Edinburgh, UK EH3 9JQ
    Nat Biotechnol 20:1240-5. 2002
    ..These findings reveal the importance of Wnt signaling in regulating ES-cell lineage diversification. More generally, this study establishes a path for rapid and direct validation of candidate genes in ES cells...
  21. doi request reprint Molecular coupling of Tsix regulation and pluripotency
    Pablo Navarro
    Unité de Génétique Moléculaire Murine, URA 2578, Institut Pasteur, 75724 Paris Cedex 15, France
    Nature 468:457-60. 2010
    ..The holistic pattern of Xist/Tsix regulation by pluripotent factors that we have identified suggests a general direct governance of complex epigenetic processes by the machinery dedicated to pluripotency...
  22. pmc Transcription factor heterogeneity and epiblast pluripotency
    Rodrigo Osorno
    Institute for Stem Cell Research, MRC Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Philos Trans R Soc Lond B Biol Sci 366:2230-7. 2011
    ..Interestingly, increasing the levels of Nanog in EpiSC can facilitate reversion to the ES cell state. Together these observations suggest that Nanog lies close to the top of the hierarchy of pluripotent transcription factor regulation...
  23. pmc The evolving biology of cell reprogramming
    Ian Wilmut
    MRC Centre for Regenerative Medicine, University of Edinburgh, Chancellor s Building, 49 Little France Crescent, Edinburgh, EH16 4SB, Scotland, UK
    Philos Trans R Soc Lond B Biol Sci 366:2183-97. 2011
    ..In this Introduction, we provide an overall context in which to consider these individual papers...
  24. doi request reprint Stem cell powwow in Squaw Valley
    Ian Chambers
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, 5 Little France Drive, Edinburgh EH16 4UU, UK
    Development 139:2457-61. 2012
    ..Here, we review the proceedings at this meeting and discuss the major advances in fundamental and applied stem cell biology that emerged...
  25. pmc Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells
    Anestis Tsakiridis
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
    Nucleic Acids Res 37:e129. 2009
    ..Taken together our data indicate that these vectors are an effective tool for insertional mutagenesis that can be used for either gene trapping or gene targeting...
  26. doi request reprint Molecular coupling of Xist regulation and pluripotency
    Pablo Navarro
    Institut Pasteur, Unité de Génétique Moléculaire Murine, CNRS, URA2578, F 75015, Paris, France
    Science 321:1693-5. 2008
    ..We conclude that the three main genetic factors underlying pluripotency cooperate to repress Xist and thus couple X inactivation reprogramming to the control of pluripotency during embryogenesis...
  27. pmc Estrogen-related receptor beta interacts with Oct4 to positively regulate Nanog gene expression
    Debbie L C van den Berg
    Department of Cell Biology, Erasmus MC, Dr Molewaterplein 50, 3015GE Rotterdam, The Netherlands
    Mol Cell Biol 28:5986-95. 2008
    ....
  28. ncbi request reprint Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles
    Giuseppe Testa
    BIOTEC, Technische Universitat Dresden, GmbH, c o Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstr 108, Dresden, Germany 01307
    Nat Biotechnol 21:443-7. 2003
    ..Combined with the use of two selectable cassettes placed far apart, BAC-based targeting constructs may be applicable to tasks such as regional exchanges, deletions, and insertions...