Research Topics
| Gregory L ChallisSummaryAffiliation: University of Warwick Country: UK Publications
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Detail Information
Publications
Mining microbial genomes for new natural products and biosynthetic pathwaysGregory L Challis
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Microbiology 154:1555-69. 2008..Our efforts to elucidate these are described. The identification of new secondary metabolites of S. coelicolor raises the question: what is their biological function? Progress towards answering this question is also summarized...- Chapter 17. Siderophore biosynthesis a substrate specificity assay for nonribosomal peptide synthetase-independent siderophore synthetases involving trapping of acyl-adenylate intermediates with hydroxylamineNadia Kadi
Department of Chemistry, University of Warwick, Coventry, United Kingdom
Methods Enzymol 458:431-57. 2009..Application of the assay to the type B NIS synthetase AcsA shows that it is selective for alpha-ketoglutaric acid, confirming a bioinformatics-based prediction of the substrate specificity of this enzyme... 
Structural basis for acyl acceptor specificity in the achromobactin biosynthetic enzyme AcsDStefan Schmelz
Scottish Structural Proteomics Facility and Centre for Biomolecular Sciences, The University of St Andrews, Scotland KY16 9ST, UK
J Mol Biol 412:495-504. 2011..That this change in specificity is accompanied by no loss in activity suggests that AcsD and other members of the non-ribosomal peptide synthetase-independent siderophore superfamily may have biotransformation potential...- Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescensMark W Silby
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Centre for Adaptation Genetics and Drug Resistance, Boston, MA 02111, USA
Genome Biol 10:R51. 2009..A functional genomic in vivo expression technology (IVET) screen provided insight into genes used by P. fluorescens in its natural environment and an improved understanding of the ecological significance of diversity within this species... - The dynamic architecture of the metabolic switch in Streptomyces coelicolorKay Nieselt
Center for Bioinformatics Tubingen, Department of Information and Cognitive Sciences, University of Tubingen, D 72076 Tubingen, Germany
BMC Genomics 11:10. 2010..We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermenter-grown samples... - Synergy and contingency as driving forces for the evolution of multiple secondary metabolite production by Streptomyces speciesGregory L Challis
Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
Proc Natl Acad Sci U S A 100:14555-61. 2003.... 
Genome mining for novel natural product discoveryGregory L Challis
Department of Chemistry, University of Warwick, Coventry CV4 7AL, U.K
J Med Chem 51:2618-28. 2008- A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetasesGregory L Challis
Department of Chemistry, Warwick University, Coventry CV4 7AL, UK
Chembiochem 6:601-11. 2005 - 2-Alkyl-4-hydroxymethylfuran-3-carboxylic acids, antibiotic production inducers discovered by Streptomyces coelicolor genome miningChristophe Corre
Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
Proc Natl Acad Sci U S A 105:17510-5. 2008.... - A new family of ATP-dependent oligomerization-macrocyclization biocatalystsNadia Kadi
Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK
Nat Chem Biol 3:652-6. 2007..This suggests that DesD is the first biochemically characterized member of a new family of oligomerizing and macrocyclizing synthetases... - Multiple biosynthetic and uptake systems mediate siderophore-dependent iron acquisition in Streptomyces coelicolor A3(2) and Streptomyces ambofaciens ATCC 23877Francisco Barona-Gomez
Department of Chemistry, University of Warwick, Coventry, UK
Microbiology 152:3355-66. 2006..Together, these results define a complex system of multiple biosynthetic and uptake pathways for siderophore-mediated iron acquisition in S. coelicolor and S. ambofaciens... - Identification of a gene cluster that directs putrebactin biosynthesis in Shewanella species: PubC catalyzes cyclodimerization of N-hydroxy-N-succinylputrescineNadia Kadi
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
J Am Chem Soc 130:10458-9. 2008.... - Distinct extracytoplasmic siderophore binding proteins recognize ferrioxamines and ferricoelichelin in Streptomyces coelicolor A3(2)Prakash Patel
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Biochemistry 49:8033-42. 2010..The remarkable specificity of the DesE and CchF proteins for different ferric-tris-hydroxamates raises intriguing questions about the molecular basis of their substrate specificity... - Oxidative tailoring reactions catalyzed by nonheme iron-dependent enzymes: streptorubin B biosynthesis as an examplePaulina K Sydor
Department of Chemistry, University of Warwick, Coventry, United Kingdom
Methods Enzymol 516:195-218. 2012.... - MbtH-like protein-mediated cross-talk between non-ribosomal peptide antibiotic and siderophore biosynthetic pathways in Streptomyces coelicolor M145Sylvie Lautru
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Microbiology 153:1405-12. 2007..Transcriptional analyses of wild-type S. coelicolor and a double cchK/cdaX replacement mutant indicate that CchK and CdaX may not be involved in transcriptional regulation of coelichelin and CDA biosynthetic gene clusters... - Regio- and stereodivergent antibiotic oxidative carbocyclizations catalysed by Rieske oxygenase-like enzymesPaulina K Sydor
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Nat Chem 3:388-92. 2011.... - A butenolide intermediate in methylenomycin furan biosynthesis is implied by incorporation of stereospecifically 13C-labelled glycerolsChristophe Corre
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
Chem Commun (Camb) 46:4079-81. 2010..The label from [3-(13)C]-L-glycerol is incorporated into the hydroxymethyl group of methylenomycin furans suggesting a butenolide intermediate in their biosynthesis... - Tailoring reactions catalyzed by heme-dependent enzymes: spectroscopic characterization of the L-tryptophan-nitrating cytochrome P450 TxtESarah M Barry
Department of Chemistry, University of Warwick, Coventry, United Kingdom
Methods Enzymol 516:171-94. 2012..Assays used to investigate substrate binding to cytochromes P450 are described using TxtE, a recently discovered unique nitrating enzyme involved in thaxtomin A biosynthesis, as an example... - Cytochrome P450–catalyzed L-tryptophan nitration in thaxtomin phytotoxin biosynthesisSarah M Barry
Department of Chemistry, University of Warwick, Coventry, UK
Nat Chem Biol 8:814-6. 2012..Here we report that TxtE, a unique cytochrome P450, catalyzes L-tryptophan nitration using NO and O(2)... - Type III polyketide synthase beta-ketoacyl-ACP starter unit and ethylmalonyl-CoA extender unit selectivity discovered by Streptomyces coelicolor genome miningLijiang Song
Department of Chemistry, University of Warwick, Coventry CV4 7AL, U.K
J Am Chem Soc 128:14754-5. 2006.... - Evidence for the unusual condensation of a diketide with a pentulose in the methylenomycin biosynthetic pathway of Streptomyces coelicolor A3(2)Christophe Corre
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
Chembiochem 6:2166-70. 2005 - Enzymatic logic of anthrax stealth siderophore biosynthesis: AsbA catalyzes ATP-dependent condensation of citric acid and spermidineDaniel Oves-Costales
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
J Am Chem Soc 129:8416-7. 2007 - Strategies for the discovery of new natural products by genome miningMalek Zerikly
Department of Chemistry, University of Warwick, Coventry, UK
Chembiochem 10:625-33. 2009..This information can be exploited in a variety of ways in the search for new bioactive natural products... - New natural product biosynthetic chemistry discovered by genome miningChristophe Corre
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Nat Prod Rep 26:977-86. 2009..Others represent novel gene clusters that direct the biosynthesis of known natural products. This article highlights several examples of new biosynthetic chemistry discovered in the course of investigating such gene clusters... - Recent advances in siderophore biosynthesisSarah M Barry
Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
Curr Opin Chem Biol 13:205-15. 2009.... - Substrate recognition by nonribosomal peptide synthetase multi-enzymesSylvie Lautru
Department of Chemistry, The University of Warwick, Coventry CV4 7AL, UK
Microbiology 150:1629-36. 2004..The situation is less clear for the thioesterase domains, which seem in certain cases to be dedicated to the hydrolysis/cyclization of their natural substrate, whereas in other cases they are largely permissive... - Heavy tools for genome miningChristophe Corre
Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
Chem Biol 14:7-9. 2007..report development of a novel genome mining method for isolating products of orphan biosynthetic gene clusters, and the application of this method to the isolation of orfamide A, a novel cyclic lipopeptide... - Mechanisms for incorporation of glycerol-derived precursors into polyketide metabolitesLaura J Walton
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
J Ind Microbiol Biotechnol 33:105-20. 2006..In this article we review the incorporation of glycerol-derived precursors into polyketides and propose new mechanisms for the incorporation processes... - Discovery of a new peptide natural product by Streptomyces coelicolor genome miningSylvie Lautru
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Nat Chem Biol 1:265-9. 2005..These results demonstrate that accurate prediction of adenylation domain substrate selectivity is possible and raise intriguing mechanistic questions regarding the assembly of a tetrapeptide by a trimodular NRPS... - Identification of a cluster of genes that directs desferrioxamine biosynthesis in Streptomyces coelicolor M145Francisco Barona-Gomez
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
J Am Chem Soc 126:16282-3. 2004.... - Non-linear enzymatic logic in natural product modular mega-synthases and -synthetasesStuart W Haynes
University of Warwick, Department of Chemistry, Coventry CV4 7AL, UK
Curr Opin Drug Discov Devel 10:203-18. 2007.... - Structural aspects of non-ribosomal peptide biosynthesisGregory L Challis
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
Curr Opin Struct Biol 14:748-56. 2004..Biochemical studies have looked at individual domains, isolated whole modules and intact NRPS, and the combined data begin to allow us to visualize the process of peptide assembly by NRPS... - Engineering Escherichia coli to produce nonribosomal peptide antibioticsGregory L Challis
Nat Chem Biol 2:398-400. 2006 - Engineering p-hydroxyphenylpyruvate dioxygenase to a p-hydroxymandelate synthase and evidence for the proposed benzene oxide intermediate in homogentisate formationMichele Gunsior
Department of Chemistry, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
Biochemistry 43:663-74. 2004..The structure of the latter was determined to be an oxepinone derived from the benzene-oxide intermediate long hypothesized in HPD catalysis... - The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081Nicholas R Thomson
The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
PLoS Genet 2:e206. 2006..Moreover, wider investigations looking at the patterns of gene loss and gain in the Yersinia have highlighted common themes in the genome evolution of other human enteropathogens... - Elucidation of the Streptomyces coelicolor pathway to 2-undecylpyrrole, a key intermediate in undecylprodiginine and streptorubin B biosynthesisSangjoon Mo
Department of Chemistry, Portland State University, Portland, OR 97207, USA
Chem Biol 15:137-48. 2008..In a redR(-) mutant, the ratio of these can be altered in a logical manner by feeding various fatty acids...