Gregory L Challis


Affiliation: University of Warwick
Country: UK


  1. Challis G. Mining microbial genomes for new natural products and biosynthetic pathways. Microbiology. 2008;154:1555-69 pubmed publisher
    ..Our efforts to elucidate these are described. The identification of new secondary metabolites of S. coelicolor raises the question: what is their biological function? Progress towards answering this question is also summarized. ..
  2. Awodi U, Ronan J, Masschelein J, de Los Santos E, Challis G. Thioester reduction and aldehyde transamination are universal steps in actinobacterial polyketide alkaloid biosynthesis. Chem Sci. 2017;8:411-415 pubmed publisher
    ..These are predicted to direct the biosynthesis of both known and novel polyketide alkaloids, suggesting that reductive chain release and transamination constitutes a conserved mechanism for the biosynthesis of such metabolites. ..
  3. Inahashi Y, Zhou S, Bibb M, Song L, Al Bassam M, Bibb M, et al. Watasemycin biosynthesis in Streptomyces venezuelae: thiazoline C-methylation by a type B radical-SAM methylase homologue. Chem Sci. 2017;8:2823-2831 pubmed publisher
  4. Liu N, Song L, Liu M, Shang F, Anderson Z, Fox D, et al. Unique post-translational oxime formation in the biosynthesis of the azolemycin complex of novel ribosomal peptides from Streptomyces sp. FXJ1.264. Chem Sci. 2016;7:482-488 pubmed publisher
    ..To the best of our knowledge, oxime formation is a hitherto unknown posttranslational modification in RiPP biosynthesis. ..
  5. Masschelein J, Jenner M, Challis G. Antibiotics from Gram-negative bacteria: a comprehensive overview and selected biosynthetic highlights. Nat Prod Rep. 2017;34:712-783 pubmed publisher
    ..We also provide a detailed discussion of several unusual pathways for antibiotic biosynthesis in Gram-negative bacteria, serving to highlight the exceptional biocatalytic repertoire of this group of microorganisms. ..
  6. Masschelein J, Clauwers C, Awodi U, Stalmans K, Vermaelen W, Lescrinier E, et al. A combination of polyunsaturated fatty acid, nonribosomal peptide and polyketide biosynthetic machinery is used to assemble the zeamine antibiotics. Chem Sci. 2015;6:923-929 pubmed publisher
    ..Thus, the zeamine antibiotics are assembled by a unique combination of nonribosomal peptide synthetase, type I modular polyketide synthase and polyunsaturated fatty acid synthase-like biosynthetic machinery. ..
  7. Challis G. Exploitation of the Streptomyces coelicolor A3(2) genome sequence for discovery of new natural products and biosynthetic pathways. J Ind Microbiol Biotechnol. 2014;41:219-32 pubmed publisher
    ..In this article, efforts to exploit the S. coelicolor genome sequence for the discovery of novel natural products and biosynthetic pathways are summarized. ..
  8. Kosol S, Jenner M, Lewandowski J, Challis G. Protein-protein interactions in trans-AT polyketide synthases. Nat Prod Rep. 2018;35:1097-1109 pubmed publisher
    ..This article summarises recent advances in understanding the protein-protein interactions underpinning subunit assembly and intra-subunit communication in such systems and highlights potential avenues and approaches for future research. ..
  9. Challis G, Hopwood D. Synergy and contingency as driving forces for the evolution of multiple secondary metabolite production by Streptomyces species. Proc Natl Acad Sci U S A. 2003;100 Suppl 2:14555-61 pubmed

More Information


  1. Perry C, de Los Santos E, Alkhalaf L, Challis G. Rieske non-heme iron-dependent oxygenases catalyse diverse reactions in natural product biosynthesis. Nat Prod Rep. 2018;35:622-632 pubmed publisher
    ..Examples of such enzymes that have yet to be experimentally investigated are also briefly described and their likely functions are discussed. ..
  2. Challis G. Genome mining for novel natural product discovery. J Med Chem. 2008;51:2618-28 pubmed publisher
  3. request reprint
    Challis G. A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases. Chembiochem. 2005;6:601-11 pubmed
  4. Ronan J, Kadi N, McMahon S, Naismith J, Alkhalaf L, Challis G. Desferrioxamine biosynthesis: diverse hydroxamate assembly by substrate-tolerant acyl transferase DesC. Philos Trans R Soc Lond B Biol Sci. 2018;373: pubmed publisher
    ..This article is part of a discussion meeting issue 'Frontiers in epigenetic chemical biology'.This article is part of a discussion meeting issue 'Frontiers in epigenetic chemical biology'. ..
  5. Zhou S, Alkhalaf L, de Los Santos E, Challis G. Mechanistic insights into class B radical-S-adenosylmethionine methylases: ubiquitous tailoring enzymes in natural product biosynthesis. Curr Opin Chem Biol. 2016;35:73-79 pubmed publisher
  6. Nieselt K, Battke F, Herbig A, Bruheim P, Wentzel A, Jakobsen Ø, et al. The dynamic architecture of the metabolic switch in Streptomyces coelicolor. BMC Genomics. 2010;11:10 pubmed publisher
    ..coelicolor, which will form the starting point for future attempts at engineering antibiotic production in a biotechnological setting. ..