Gregory L Challis

Summary

Affiliation: University of Warwick
Country: UK

Publications

  1. ncbi request reprint Mining microbial genomes for new natural products and biosynthetic pathways
    Gregory L Challis
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Microbiology 154:1555-69. 2008
  2. doi request reprint Chapter 17. Siderophore biosynthesis a substrate specificity assay for nonribosomal peptide synthetase-independent siderophore synthetases involving trapping of acyl-adenylate intermediates with hydroxylamine
    Nadia Kadi
    Department of Chemistry, University of Warwick, Coventry, United Kingdom
    Methods Enzymol 458:431-57. 2009
  3. pmc Regio- and stereodivergent antibiotic oxidative carbocyclizations catalysed by Rieske oxygenase-like enzymes
    Paulina K Sydor
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Nat Chem 3:388-92. 2011
  4. pmc Cytochrome P450–catalyzed L-tryptophan nitration in thaxtomin phytotoxin biosynthesis
    Sarah M Barry
    Department of Chemistry, University of Warwick, Coventry, UK
    Nat Chem Biol 8:814-6. 2012
  5. pmc The insect pathogen Serratia marcescens Db10 uses a hybrid non-ribosomal peptide synthetase-polyketide synthase to produce the antibiotic althiomycin
    Amy J Gerc
    Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee, United Kingdom
    PLoS ONE 7:e44673. 2012
  6. ncbi request reprint Exploitation of the Streptomyces coelicolor A3(2) genome sequence for discovery of new natural products and biosynthetic pathways
    Gregory L Challis
    Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
    J Ind Microbiol Biotechnol 41:219-32. 2014
  7. pmc Structural basis for acyl acceptor specificity in the achromobactin biosynthetic enzyme AcsD
    Stefan Schmelz
    Scottish Structural Proteomics Facility and Centre for Biomolecular Sciences, The University of St Andrews, Scotland KY16 9ST, UK
    J Mol Biol 412:495-504. 2011
  8. pmc Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens
    Mark W Silby
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Centre for Adaptation Genetics and Drug Resistance, Boston, MA 02111, USA
    Genome Biol 10:R51. 2009
  9. pmc The dynamic architecture of the metabolic switch in Streptomyces coelicolor
    Kay Nieselt
    Center for Bioinformatics Tubingen, Department of Information and Cognitive Sciences, University of Tubingen, D 72076 Tubingen, Germany
    BMC Genomics 11:10. 2010
  10. doi request reprint Genome mining for novel natural product discovery
    Gregory L Challis
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, U K
    J Med Chem 51:2618-28. 2008

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Mining microbial genomes for new natural products and biosynthetic pathways
    Gregory L Challis
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Microbiology 154:1555-69. 2008
    ..Our efforts to elucidate these are described. The identification of new secondary metabolites of S. coelicolor raises the question: what is their biological function? Progress towards answering this question is also summarized...
  2. doi request reprint Chapter 17. Siderophore biosynthesis a substrate specificity assay for nonribosomal peptide synthetase-independent siderophore synthetases involving trapping of acyl-adenylate intermediates with hydroxylamine
    Nadia Kadi
    Department of Chemistry, University of Warwick, Coventry, United Kingdom
    Methods Enzymol 458:431-57. 2009
    ..Application of the assay to the type B NIS synthetase AcsA shows that it is selective for alpha-ketoglutaric acid, confirming a bioinformatics-based prediction of the substrate specificity of this enzyme...
  3. pmc Regio- and stereodivergent antibiotic oxidative carbocyclizations catalysed by Rieske oxygenase-like enzymes
    Paulina K Sydor
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Nat Chem 3:388-92. 2011
    ....
  4. pmc Cytochrome P450–catalyzed L-tryptophan nitration in thaxtomin phytotoxin biosynthesis
    Sarah M Barry
    Department of Chemistry, University of Warwick, Coventry, UK
    Nat Chem Biol 8:814-6. 2012
    ..Here we report that TxtE, a unique cytochrome P450, catalyzes L-tryptophan nitration using NO and O(2)...
  5. pmc The insect pathogen Serratia marcescens Db10 uses a hybrid non-ribosomal peptide synthetase-polyketide synthase to produce the antibiotic althiomycin
    Amy J Gerc
    Division of Molecular Microbiology, College of Life Sciences, University of Dundee, Dundee, United Kingdom
    PLoS ONE 7:e44673. 2012
    ..marcescens represents a convenient and tractable producing organism...
  6. ncbi request reprint Exploitation of the Streptomyces coelicolor A3(2) genome sequence for discovery of new natural products and biosynthetic pathways
    Gregory L Challis
    Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
    J Ind Microbiol Biotechnol 41:219-32. 2014
    ..In this article, efforts to exploit the S. coelicolor genome sequence for the discovery of novel natural products and biosynthetic pathways are summarized. ..
  7. pmc Structural basis for acyl acceptor specificity in the achromobactin biosynthetic enzyme AcsD
    Stefan Schmelz
    Scottish Structural Proteomics Facility and Centre for Biomolecular Sciences, The University of St Andrews, Scotland KY16 9ST, UK
    J Mol Biol 412:495-504. 2011
    ..That this change in specificity is accompanied by no loss in activity suggests that AcsD and other members of the non-ribosomal peptide synthetase-independent siderophore superfamily may have biotransformation potential...
  8. pmc Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens
    Mark W Silby
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Centre for Adaptation Genetics and Drug Resistance, Boston, MA 02111, USA
    Genome Biol 10:R51. 2009
    ..A functional genomic in vivo expression technology (IVET) screen provided insight into genes used by P. fluorescens in its natural environment and an improved understanding of the ecological significance of diversity within this species...
  9. pmc The dynamic architecture of the metabolic switch in Streptomyces coelicolor
    Kay Nieselt
    Center for Bioinformatics Tubingen, Department of Information and Cognitive Sciences, University of Tubingen, D 72076 Tubingen, Germany
    BMC Genomics 11:10. 2010
    ..We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermenter-grown samples...
  10. doi request reprint Genome mining for novel natural product discovery
    Gregory L Challis
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, U K
    J Med Chem 51:2618-28. 2008
  11. pmc Synergy and contingency as driving forces for the evolution of multiple secondary metabolite production by Streptomyces species
    Gregory L Challis
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
    Proc Natl Acad Sci U S A 100:14555-61. 2003
    ....
  12. ncbi request reprint A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases
    Gregory L Challis
    Department of Chemistry, Warwick University, Coventry CV4 7AL, UK
    Chembiochem 6:601-11. 2005
  13. pmc 2-Alkyl-4-hydroxymethylfuran-3-carboxylic acids, antibiotic production inducers discovered by Streptomyces coelicolor genome mining
    Christophe Corre
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
    Proc Natl Acad Sci U S A 105:17510-5. 2008
    ....
  14. ncbi request reprint A new family of ATP-dependent oligomerization-macrocyclization biocatalysts
    Nadia Kadi
    Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK
    Nat Chem Biol 3:652-6. 2007
    ..This suggests that DesD is the first biochemically characterized member of a new family of oligomerizing and macrocyclizing synthetases...
  15. pmc AcsD catalyzes enantioselective citrate desymmetrization in siderophore biosynthesis
    Stefan Schmelz
    Scottish Structural Proteomics Facility and Centre for Biomolecular Sciences, The University of St Andrews, Scotland KY16 9ST, UK
    Nat Chem Biol 5:174-82. 2009
    ..AcsD is a unique acyladenylate-forming enzyme with a new fold and chemical catalysis strategy...
  16. ncbi request reprint Multiple biosynthetic and uptake systems mediate siderophore-dependent iron acquisition in Streptomyces coelicolor A3(2) and Streptomyces ambofaciens ATCC 23877
    Francisco Barona-Gomez
    Department of Chemistry, University of Warwick, Coventry, UK
    Microbiology 152:3355-66. 2006
    ..Together, these results define a complex system of multiple biosynthetic and uptake pathways for siderophore-mediated iron acquisition in S. coelicolor and S. ambofaciens...
  17. ncbi request reprint Distinct extracytoplasmic siderophore binding proteins recognize ferrioxamines and ferricoelichelin in Streptomyces coelicolor A3(2)
    Prakash Patel
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Biochemistry 49:8033-42. 2010
    ..The remarkable specificity of the DesE and CchF proteins for different ferric-tris-hydroxamates raises intriguing questions about the molecular basis of their substrate specificity...
  18. ncbi request reprint Identification of a gene cluster that directs putrebactin biosynthesis in Shewanella species: PubC catalyzes cyclodimerization of N-hydroxy-N-succinylputrescine
    Nadia Kadi
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    J Am Chem Soc 130:10458-9. 2008
    ....
  19. doi request reprint Tailoring reactions catalyzed by heme-dependent enzymes: spectroscopic characterization of the L-tryptophan-nitrating cytochrome P450 TxtE
    Sarah M Barry
    Department of Chemistry, University of Warwick, Coventry, United Kingdom
    Methods Enzymol 516:171-94. 2012
    ..Assays used to investigate substrate binding to cytochromes P450 are described using TxtE, a recently discovered unique nitrating enzyme involved in thaxtomin A biosynthesis, as an example...
  20. ncbi request reprint A butenolide intermediate in methylenomycin furan biosynthesis is implied by incorporation of stereospecifically 13C-labelled glycerols
    Christophe Corre
    Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
    Chem Commun (Camb) 46:4079-81. 2010
    ..The label from [3-(13)C]-L-glycerol is incorporated into the hydroxymethyl group of methylenomycin furans suggesting a butenolide intermediate in their biosynthesis...
  21. doi request reprint Oxidative tailoring reactions catalyzed by nonheme iron-dependent enzymes: streptorubin B biosynthesis as an example
    Paulina K Sydor
    Department of Chemistry, University of Warwick, Coventry, United Kingdom
    Methods Enzymol 516:195-218. 2012
    ....
  22. pmc Type III polyketide synthase beta-ketoacyl-ACP starter unit and ethylmalonyl-CoA extender unit selectivity discovered by Streptomyces coelicolor genome mining
    Lijiang Song
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, U K
    J Am Chem Soc 128:14754-5. 2006
    ....
  23. ncbi request reprint MbtH-like protein-mediated cross-talk between non-ribosomal peptide antibiotic and siderophore biosynthetic pathways in Streptomyces coelicolor M145
    Sylvie Lautru
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Microbiology 153:1405-12. 2007
    ..Transcriptional analyses of wild-type S. coelicolor and a double cchK/cdaX replacement mutant indicate that CchK and CdaX may not be involved in transcriptional regulation of coelichelin and CDA biosynthetic gene clusters...
  24. ncbi request reprint Discovery of a new peptide natural product by Streptomyces coelicolor genome mining
    Sylvie Lautru
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Nat Chem Biol 1:265-9. 2005
    ..These results demonstrate that accurate prediction of adenylation domain substrate selectivity is possible and raise intriguing mechanistic questions regarding the assembly of a tetrapeptide by a trimodular NRPS...
  25. ncbi request reprint Enzymatic logic of anthrax stealth siderophore biosynthesis: AsbA catalyzes ATP-dependent condensation of citric acid and spermidine
    Daniel Oves-Costales
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    J Am Chem Soc 129:8416-7. 2007
  26. doi request reprint Recent advances in siderophore biosynthesis
    Sarah M Barry
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
    Curr Opin Chem Biol 13:205-15. 2009
    ....
  27. ncbi request reprint Strategies for the discovery of new natural products by genome mining
    Malek Zerikly
    Department of Chemistry, University of Warwick, Coventry, UK
    Chembiochem 10:625-33. 2009
    ..This information can be exploited in a variety of ways in the search for new bioactive natural products...
  28. doi request reprint Cytochrome P450-mediated hydroxylation is required for polyketide macrolactonization in stambomycin biosynthesis
    Lijiang Song
    Department of Chemistry, University of Warwick, Coventry, UK
    J Antibiot (Tokyo) 67:71-6. 2014
    ..This is a novel mechanism for macrolactone formation in polyketide antibiotic biosynthesis. ..
  29. ncbi request reprint Evidence for the unusual condensation of a diketide with a pentulose in the methylenomycin biosynthetic pathway of Streptomyces coelicolor A3(2)
    Christophe Corre
    Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
    Chembiochem 6:2166-70. 2005
  30. ncbi request reprint Identification of a cluster of genes that directs desferrioxamine biosynthesis in Streptomyces coelicolor M145
    Francisco Barona-Gomez
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    J Am Chem Soc 126:16282-3. 2004
    ....
  31. ncbi request reprint Mechanisms for incorporation of glycerol-derived precursors into polyketide metabolites
    Laura J Walton
    Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK
    J Ind Microbiol Biotechnol 33:105-20. 2006
    ..In this article we review the incorporation of glycerol-derived precursors into polyketides and propose new mechanisms for the incorporation processes...
  32. ncbi request reprint New natural product biosynthetic chemistry discovered by genome mining
    Christophe Corre
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Nat Prod Rep 26:977-86. 2009
    ..Others represent novel gene clusters that direct the biosynthesis of known natural products. This article highlights several examples of new biosynthetic chemistry discovered in the course of investigating such gene clusters...
  33. ncbi request reprint Non-linear enzymatic logic in natural product modular mega-synthases and -synthetases
    Stuart W Haynes
    University of Warwick, Department of Chemistry, Coventry CV4 7AL, UK
    Curr Opin Drug Discov Devel 10:203-18. 2007
    ....
  34. ncbi request reprint Heavy tools for genome mining
    Christophe Corre
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
    Chem Biol 14:7-9. 2007
    ..report development of a novel genome mining method for isolating products of orphan biosynthetic gene clusters, and the application of this method to the isolation of orfamide A, a novel cyclic lipopeptide...
  35. pmc Structural aspects of non-ribosomal peptide biosynthesis
    Gregory L Challis
    Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
    Curr Opin Struct Biol 14:748-56. 2004
    ..Biochemical studies have looked at individual domains, isolated whole modules and intact NRPS, and the combined data begin to allow us to visualize the process of peptide assembly by NRPS...
  36. ncbi request reprint Substrate recognition by nonribosomal peptide synthetase multi-enzymes
    Sylvie Lautru
    Department of Chemistry, The University of Warwick, Coventry CV4 7AL, UK
    Microbiology 150:1629-36. 2004
    ..The situation is less clear for the thioesterase domains, which seem in certain cases to be dedicated to the hydrolysis/cyclization of their natural substrate, whereas in other cases they are largely permissive...
  37. ncbi request reprint Engineering Escherichia coli to produce nonribosomal peptide antibiotics
    Gregory L Challis
    Nat Chem Biol 2:398-400. 2006
  38. ncbi request reprint Engineering p-hydroxyphenylpyruvate dioxygenase to a p-hydroxymandelate synthase and evidence for the proposed benzene oxide intermediate in homogentisate formation
    Michele Gunsior
    Department of Chemistry, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Biochemistry 43:663-74. 2004
    ..The structure of the latter was determined to be an oxepinone derived from the benzene-oxide intermediate long hypothesized in HPD catalysis...
  39. pmc The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081
    Nicholas R Thomson
    The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Genet 2:e206. 2006
    ..Moreover, wider investigations looking at the patterns of gene loss and gain in the Yersinia have highlighted common themes in the genome evolution of other human enteropathogens...
  40. ncbi request reprint Elucidation of the Streptomyces coelicolor pathway to 2-undecylpyrrole, a key intermediate in undecylprodiginine and streptorubin B biosynthesis
    Sangjoon Mo
    Department of Chemistry, Portland State University, Portland, OR 97207, USA
    Chem Biol 15:137-48. 2008
    ..In a redR(-) mutant, the ratio of these can be altered in a logical manner by feeding various fatty acids...