Affiliation: University of Durham
Linthwaite V, Janus J, Brown A, Wong Pascua D, O Donoghue A, Porter A, et al
. The identification of carbon dioxide mediated protein post-translational modifications. Nat Commun. 2018;9:3092 pubmed publisher
..The ability to identify protein carbamates will significantly advance our understanding of cellular CO2 interactions. ..
Townsend P, Rodgers T, Glover L, Korhonen H, Richards S, Colwell L, et al
. The Role of Protein-Ligand Contacts in Allosteric Regulation of the Escherichia coli Catabolite Activator Protein. J Biol Chem. 2015;290:22225-35 pubmed publisher
..In general, we hypothesize an evolutionary selection pressure to retain slow relaxation dynamics-induced allostery in proteins in which evolution of the ligand-binding site is occurring. ..
Fenyk S, Townsend P, Dixon C, Spies G, de San Eustaquio Campillo A, Slootweg E, et al
. The Potato Nucleotide-binding Leucine-rich Repeat (NLR) Immune Receptor Rx1 Is a Pathogen-dependent DNA-deforming Protein. J Biol Chem. 2015;290:24945-60 pubmed publisher
..DNA binding is therefore not merely a consequence of immune activation. These data establish a role for DNA distortion in Rx1 immune signaling and define DNA as a molecular target of an activated NLR. ..
Fenyk S, Dixon C, Gittens W, Townsend P, Sharples G, PÃ¥lsson L, et al
. The Tomato Nucleotide-binding Leucine-rich Repeat Immune Receptor I-2 Couples DNA-binding to Nucleotide-binding Domain Nucleotide Exchange. J Biol Chem. 2016;291:1137-47 pubmed publisher
..Thus DNA binding is a general property of at least a subset of NLR proteins, and NLR activation is directly linked to its activity at DNA. ..
Townsend P, Dixon C, Slootweg E, Sukarta O, Yang A, Hughes T, et al
. The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor. J Biol Chem. 2018;293:3218-3233 pubmed publisher
..This study provides new mechanistic insight into how an NLR can coordinate an immune signaling response at DNA following pathogen perceptions. ..
Townsend P, Rodgers T, Pohl E, Wilson M, McLeish T, Cann M. Global low-frequency motions in protein allostery: CAP as a model system. Biophys Rev. 2015;7:175-182 pubmed publisher
Cann M, Hammer A, Zhou J, Kanacher T. A defined subset of adenylyl cyclases is regulated by bicarbonate ion. J Biol Chem. 2003;278:35033-8 pubmed
..Large numbers of class III adenylyl cyclases may therefore be activated by bicarbonate. This represents a novel mechanism by which diverse organisms can detect bicarbonate ion. ..
Cann M. A subset of GAF domains are evolutionarily conserved sodium sensors. Mol Microbiol. 2007;64:461-72 pubmed
..CyaB1 and CyaB2 are the first identified directly Na(+)-responsive signalling molecules that function in sodium homeostasis and we propose a subset of GAF domains underpin an evolutionarily conserved Na(+) signalling mechanism. ..
Cann M. Sodium regulation of GAF domain function. Biochem Soc Trans. 2007;35:1032-4 pubmed
..Sodium regulation of GAF domain function has therefore been conserved since the eukaryotic/prokaryotic divergence. The GAF domain is the first identified protein domain to directly sense and signal changes in environmental sodium. ..