Genomes and Genes
M A Caldwell
Affiliation: University of Cambridge
- Neural precursor cell influences on blood-brain barrier characteristics in rat brain endothelial cellsJoseph C Lim
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
Brain Res 1159:67-76. 2007..Similar increases were observed in co-culture with astrocytes. These results suggest that undifferentiated NPCs produce factors that influence Pgp expression whereas their progeny also affect tight junction integrity...
- Identification of unique reciprocal and non reciprocal cross packaging relationships between HIV-1, HIV-2 and SIV reveals an efficient SIV/HIV-2 lentiviral vector system with highly favourable features for in vivo testing and clinical usagePadraig M Strappe
Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge CB2 2QQ, UK
Retrovirology 2:55. 2005..Cross-packaged vectors expressing GFP were assessed for RNA packaging, viral vector titre and their ability to transduce rat primary glial cell cultures and human neural stem cells...
- 5-Bromo-2'-deoxyuridine is selectively toxic to neuronal precursors in vitroMaeve A Caldwell
Centre for Brain Repair and Department of Clinical Neurosciences, Cambridge University Forvie Site, Robinson Way, Cambridge CB2 2PY, UK
Eur J Neurosci 22:2965-70. 2005..Our results show that high levels of BrdU are selectively toxic to neurons through a mechanism that activates classical cell death pathways. This has implications for labelling studies both in vivo and in vitro...
- Heparin, but not other proteoglycans potentiates the mitogenic effects of FGF-2 on mesencephalic precursor cellsM A Caldwell
MRC Cambridge Centre for Brain Repair, Cambridge University Forvie Site, Robinson Way, Cambridge, CB2 2PY, England
Exp Neurol 152:1-10. 1998..We conclude that heparin, but not other closely related proteoglycans, is vital for the growth of FGF-2-responsive mesencephalic neural precursors...
- Heparin stabilizes FGF-2 and modulates striatal precursor cell behavior in response to EGFMaeve A Caldwell
Centre for Brain Repair, Cambridge University Forvie Site, Robinson Way, CB2 2PY, UK
Exp Neurol 188:408-20. 2004....
- Growth factors regulate the survival and fate of cells derived from human neurospheresM A Caldwell
MRC Centre for Brain Repair, University of Cambridge, Forvie Site, Robinson Way, Cambridge, CB2 2PY, UK
Nat Biotechnol 19:475-9. 2001..These should be useful for both neuroactive drug screening in vitro and possibly cell therapy for neurodegenerative diseases...
- Long-term survival of human central nervous system progenitor cells transplanted into a rat model of Parkinson's diseaseC N Svendsen
MRC Cambridge Centre for Brain Repair, Cambridge University Forvie Site, United Kingdom
Exp Neurol 148:135-46. 1997..As such these cells may have potential for development as an alternative source of tissue for neural transplantation in degenerative diseases...
- Human neural precursor cells express low levels of telomerase in vitro and show diminishing cell proliferation with extensive axonal outgrowth following transplantationT Ostenfeld
MRC Centre for Brain Repair, University of Cambridge, United Kingdom
Exp Neurol 164:215-26. 2000....
- A new method for the rapid and long term growth of human neural precursor cellsC N Svendsen
MRC Cambridge Centre for Brain Repair, Cambridge University Forvie Site, Robinson Way, UK
J Neurosci Methods 85:141-52. 1998..This simple and novel culture method allows the rapid expansion of large numbers of non-transformed human neural precursor cells which may be of use in drug discovery, ex vivo gene therapy and clinical neural transplantation...
- Human neural stem cells: isolation, expansion and transplantationC N Svendsen
MRC Cambridge Centre for Brain Repair, University of Cambridge, UK
Brain Pathol 9:499-513. 1999..This review summarises this fascinating and growing field of neurobiology, with a particular focus on human tissues...
- Astrocytes promote neurogenesis from oligodendrocyte precursor cellsP M Gaughwin
Department of Clinical Neurosciences and Centre for Brain Repair, Forvie Site, Robinson Way, Cambridge CB2 2PY, UK
Eur J Neurosci 23:945-56. 2006..The present findings show that hippocampal, astrocyte-derived signals can induce the neuronal differentiation of OPCs through a Noggin-independent mechanism...
- Improving the survival of human CNS precursor-derived neurons after transplantationJ E Le Belle
Cambridge Centre for Brain Repair, University of Cambridge, Cambridge, United Kingdom
J Neurosci Res 76:174-83. 2004..Undifferentiated HNPCs migrated more widely in the brain after grafting than did the predifferentiated cells, which generally remained within the hippocampus...
- Antioxidant strategy to counteract the side effects of antipsychotic therapy: an in vivo study in ratsL Besret
Centre for Brain Repair, University of Cambridge, Cambridge, UK
Eur J Pharmacol 408:35-9. 2000..Moreover, the current study has shown the effect of the antioxidant vitamin C in protecting haloperidol effects on tyrosine hydroxylase-immunostaining...
- Olfactory bulb hypoplasia in Prokr2 null mice stems from defective neuronal progenitor migration and differentiationHaydn M Prosser
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Eur J Neurosci 26:3339-44. 2007..Together, these findings suggest an important role for Prokr2 in OB neurogenesis...
- Contrasting effects of basic fibroblast growth factor and epidermal growth factor on mouse neonatal olfactory mucosa cellsPerrine Barraud
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
Eur J Neurosci 26:3345-57. 2007..In contrast, EGF appears to be primarily a survival factor for both the olfactory stem and precursor cells...
- Induction of A9 dopaminergic neurons from neural stem cells improves motor function in an animal model of Parkinson's diseaseFiona E O'Keeffe
Cambridge Center for Brain Repair and Department of Clinical Neurosciences, Forvie Site, Robinsons Way, Cambridge CB2 2PY, UK
Brain 131:630-41. 2008..This demonstrates that given the correct signals, NSCs can be induced to become dopaminergic neurons that can differentiate into the correct nigrastriatal phenotype required for the treatment of Parkinson's disease...
- Secreted factors from olfactory mucosa cells expanded as free-floating spheres increase neurogenesis in olfactory bulb neurosphere culturesPerrine Barraud
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
BMC Neurosci 9:24. 2008..However, olfactory stem and progenitor cells expand very slowly when grown as free-floating spheres (olfactory-spheres) under growth factor stimulation in a neurosphere assay...
- Patterns of laminins and integrins in the embryonic ventricular zone of the CNSJustin D Lathia
Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA
J Comp Neurol 505:630-43. 2007....
- Human neural progenitor cell transplants into the subthalamic nucleus lead to functional recovery in a rat model of Parkinson's diseaseLucy Anderson
Centre for Brain Repair and Department of Clinical Neurosciences, University Forvie Site, Robinson Way, Cambridge, CB2 2PY, UK
Neurobiol Dis 27:133-40. 2007..This suggests that even in the absence of cell replacement strategies utilising immature progenitor cells could be of real therapeutic value...
- Environmental signals regulate lineage choice and temporal maturation of neural stem cells from human embryonic stem cellsAlexis J Joannides
Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK
Brain 130:1263-75. 2007..Controlled manipulation of environmental signals appropriate to the pathological specificity of the targeted disease will be necessary in the design of therapeutic stem cell-based strategies...
- Gene expression changes in long term expanded human neural progenitor cells passaged by chopping lead to loss of neurogenic potential in vivoLucy Anderson
Centre for Brain Repair and Department of Clinical Neurosciences, University Forvie Site, Robinson Way, Cambridge CB2 2PY, UK
Exp Neurol 204:512-24. 2007..Array analysis confirms that following extensive time in culture cells are entering growth arrest with fundamental expression changes in genes associated with cell cycle regulation, apoptosis and immune functions...
- Integrins are markers of human neural stem cellsPeter E Hall
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom
Stem Cells 24:2078-84. 2006..Therefore, integrin subunits alpha6 and beta1 are highly expressed by human neural precursors and represent convenient markers for their prospective isolation...
- Differentiation and migration of long term expanded human neural progenitors in a partial lesion model of Parkinson's diseaseRowan M Burnstein
Cambridge Centre for Brain Repair, University of Cambridge, Forvie Site, Robinson Way, Cambridge CB2 2PY, UK
Int J Biochem Cell Biol 36:702-13. 2004..There was no significant behavioural recovery or effect of transplanted HNPCs on the loss of dopaminergic cells from the host brain. In conclusion, HNPCs may provide a source of cells for use in the treatment of Parkinson's disease...
- Modern therapeutic approaches in Parkinson's diseaseSimon J G Lewis
Cambridge Centre for Brain Repair, Forvie Site, Robinson Way, Cambridge, CB2 2PY, UK
Expert Rev Mol Med 5:1-20. 2003..In this review, we discuss the emerging new therapies in PD in terms of neuroprotective agents, drugs designed to control symptoms more effectively, and finally curative cell therapies...
- Gene expression in human neural stem cells: effects of leukemia inhibitory factorLynda S Wright
The Waisman Center Stem Cell Research Program and Department of Anatomy, University of Wisconsin, 53705, USA
J Neurochem 86:179-95. 2003..This study provides the first molecular profile of human ltNSCctx cultures capable of long-term self renewal, and reveals specific sets of genes that are directly or indirectly regulated by LIF...
- Neuronal target genes of the neuron-restrictive silencer factor in neurospheres derived from fetuses with Down's syndrome: a gene expression studySabine Bahn
Department of Neurobiology, Babraham Institute, Cambridge CB2 4AT, UK
Lancet 359:310-5. 2002..We did a study to investigate abnormalities in gene expression in human neuronal stem cells and progenitor cells from Down's syndrome and control post-mortem human fetal tissue...
- Laminin enhances the growth of human neural stem cells in defined culture mediaPeter E Hall
Department of Pathology, University of Cambridge, Cambridge, UK
BMC Neurosci 9:71. 2008..A common feature of niches is the presence of the extracellular matrix molecule, laminin. Therefore, this study investigated the effect of exogenous laminin on hNSC growth...
- Neurodegeneration and cell replacementBrandi K Ormerod
Department of Neurosurgery, Stanford University, 300 Pasteur Drive, Boswell Building, A301, Stanford, CA 94305 5327, USA
Philos Trans R Soc Lond B Biol Sci 363:153-70. 2008....