R D Burgoyne

Summary

Affiliation: University of Liverpool
Country: UK

Publications

  1. pmc Identification of common genetic modifiers of neurodegenerative diseases from an integrative analysis of diverse genetic screens in model organisms
    Xi Chen
    Department of Cellular and Molecular Physiology, Physiological Laboratory, Institute of Translational Medicine, University of Liverpool, Crown St, Liverpool L69 3BX, UK
    BMC Genomics 13:71. 2012
  2. pmc Understanding the physiological roles of the neuronal calcium sensor proteins
    Robert D Burgoyne
    Department of Cellular and Molecular Physiology, The Physiological Laboratory, Institute of Translational Medicine, University of Liverpool, Liverpool, UK
    Mol Brain 5:2. 2012
  3. pmc Bioinformatic analysis of CaBP/calneuron proteins reveals a family of highly conserved vertebrate Ca2+-binding proteins
    Hannah V McCue
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    BMC Res Notes 3:118. 2010
  4. ncbi request reprint Calcium and calmodulin in membrane fusion
    Robert D Burgoyne
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Biochim Biophys Acta 1641:137-43. 2003
  5. pmc The neuronal calcium sensor family of Ca2+-binding proteins
    R D Burgoyne
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Biochem J 353:1-12. 2001
  6. doi request reprint The functions of Munc18-1 in regulated exocytosis
    Robert D Burgoyne
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool, United Kingdom
    Ann N Y Acad Sci 1152:76-86. 2009
  7. ncbi request reprint Chaperoning the SNAREs: a role in preventing neurodegeneration?
    Robert D Burgoyne
    Nat Cell Biol 13:8-9. 2011
  8. pmc Neuronal calcium sensor proteins: generating diversity in neuronal Ca2+ signalling
    Robert D Burgoyne
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, UK
    Nat Rev Neurosci 8:182-93. 2007
  9. pmc Distinct effects of alpha-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis
    L H Chamberlain
    Physiological Laboratory, University of Liverpool, United Kingdom
    J Cell Biol 130:1063-70. 1995
  10. ncbi request reprint Neuronal Ca(2+) sensor 1. Characterization of the myristoylated protein, its cellular effects in permeabilized adrenal chromaffin cells, Ca(2+)-independent membrane association, and interaction with binding proteins, suggesting a role in rapid Ca(2+) sign
    B W McFerran
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 274:30258-65. 1999

Collaborators

Detail Information

Publications84

  1. pmc Identification of common genetic modifiers of neurodegenerative diseases from an integrative analysis of diverse genetic screens in model organisms
    Xi Chen
    Department of Cellular and Molecular Physiology, Physiological Laboratory, Institute of Translational Medicine, University of Liverpool, Crown St, Liverpool L69 3BX, UK
    BMC Genomics 13:71. 2012
    ..There is, however, a need for a fully integrated analysis of the available genetic data based on careful comparison of orthologues across the species to determine the real extent of overlap...
  2. pmc Understanding the physiological roles of the neuronal calcium sensor proteins
    Robert D Burgoyne
    Department of Cellular and Molecular Physiology, The Physiological Laboratory, Institute of Translational Medicine, University of Liverpool, Liverpool, UK
    Mol Brain 5:2. 2012
    ..Here we review recent advances in the understanding of the physiological roles of the NCS proteins and the molecular basis for their specificity...
  3. pmc Bioinformatic analysis of CaBP/calneuron proteins reveals a family of highly conserved vertebrate Ca2+-binding proteins
    Hannah V McCue
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    BMC Res Notes 3:118. 2010
    ..These were suggested to be vertebrate specific but exactly which family members are expressed outside of mammalian species had not been examined...
  4. ncbi request reprint Calcium and calmodulin in membrane fusion
    Robert D Burgoyne
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Biochim Biophys Acta 1641:137-43. 2003
    ..The extent to which calmodulin regulates different intracellular fusion events through conserved SNARE-dependent or other mechanisms remains to be resolved...
  5. pmc The neuronal calcium sensor family of Ca2+-binding proteins
    R D Burgoyne
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Biochem J 353:1-12. 2001
    ..In the present review we describe the known sequences and structures of the NCS proteins, information on their interactions with target proteins and current knowledge about their cellular and physiological functions...
  6. doi request reprint The functions of Munc18-1 in regulated exocytosis
    Robert D Burgoyne
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool, United Kingdom
    Ann N Y Acad Sci 1152:76-86. 2009
    ....
  7. ncbi request reprint Chaperoning the SNAREs: a role in preventing neurodegeneration?
    Robert D Burgoyne
    Nat Cell Biol 13:8-9. 2011
    ....
  8. pmc Neuronal calcium sensor proteins: generating diversity in neuronal Ca2+ signalling
    Robert D Burgoyne
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, UK
    Nat Rev Neurosci 8:182-93. 2007
    ..The differing properties of these proteins allow them to have discrete, non-redundant functions...
  9. pmc Distinct effects of alpha-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis
    L H Chamberlain
    Physiological Laboratory, University of Liverpool, United Kingdom
    J Cell Biol 130:1063-70. 1995
    ....
  10. ncbi request reprint Neuronal Ca(2+) sensor 1. Characterization of the myristoylated protein, its cellular effects in permeabilized adrenal chromaffin cells, Ca(2+)-independent membrane association, and interaction with binding proteins, suggesting a role in rapid Ca(2+) sign
    B W McFerran
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 274:30258-65. 1999
    ..This differs from the Ca(2+) myristoyl switch model for the closely related recoverin and suggests a possible action in rapid Ca(2+) signal transduction in response to local Ca(2+) signals...
  11. ncbi request reprint Control of membrane fusion dynamics during regulated exocytosis
    R D Burgoyne
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Biochem Soc Trans 29:467-72. 2001
    ..These studies have demonstrated a late role for cysteine string protein in exocytosis. In addition, they have focused attention on a key role for Munc18 in the regulation of post-fusion events that affect fusion pore dynamics...
  12. ncbi request reprint Voltage-independent inhibition of P/Q-type Ca2+ channels in adrenal chromaffin cells via a neuronal Ca2+ sensor-1-dependent pathway involves Src family tyrosine kinase
    J L Weiss
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 276:44804-11. 2001
    ....
  13. pmc Identification of Exo2 as the catalytic subunit of protein kinase A reveals a role for cyclic AMP in Ca(2+)-dependent exocytosis in chromaffin cells
    A Morgan
    Department of Physiology, University of Liverpool, UK
    EMBO J 12:3747-52. 1993
    ..These data show that PKA (Exo2) regulates Ca(2+)-dependent exocytosis in bovine adrenal chromaffin cells...
  14. ncbi request reprint Control of fusion pore dynamics during exocytosis by Munc18
    R J Fisher
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK
    Science 291:875-8. 2001
    ..Thus, Munc18 functions in a late stage in the fusion process, where its dissociation from syntaxin determines the kinetics of postfusion events...
  15. ncbi request reprint Distribution of two distinct Ca2+-ATPase-like proteins and their relationships to the agonist-sensitive calcium store in adrenal chromaffin cells
    R D Burgoyne
    Physiological Laboratory, University of Liverpool, UK
    Nature 342:72-4. 1989
    ..Our results indicate that chromaffin cells possess two calcium stores with distinct Ca2+-ATPases and that the organelle with the 100K Ca2+-ATPase is not the Ins(1,4,5)P3-sensitive store...
  16. ncbi request reprint A synthetic peptide of the N-terminus of ADP-ribosylation factor (ARF) inhibits regulated exocytosis in adrenal chromaffin cells
    A Morgan
    Physiological Laboratory, University of Liverpool, UK
    FEBS Lett 329:121-4. 1993
    ..These results point to a hitherto unsuspected role for ARF in regulated exocytosis, and the potency of the hARF1(2-17) peptide suggests that ARF is essential for exocytosis in bovine adrenal chromaffin cells...
  17. ncbi request reprint Phosphorylation of cysteine string protein by protein kinase A. Implications for the modulation of exocytosis
    G J Evans
    Physiological Laboratory and School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 276:47877-85. 2001
    ..We propose that PKA phosphorylation of CSP could modulate the exocytotic machinery, by selectively altering its availability for protein-protein interactions...
  18. ncbi request reprint A direct inhibitory role for the Rab3-specific effector, Noc2, in Ca2+-regulated exocytosis in neuroendocrine cells
    L P Haynes
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 276:9726-32. 2001
    ..We propose that Noc2 may be a negative effector for Rab3A in regulated exocytosis of dense-core granules from endocrine cells...
  19. ncbi request reprint SNAP-25 with mutations in the zero layer supports normal membrane fusion kinetics
    M E Graham
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK
    J Cell Sci 114:4397-405. 2001
    ....
  20. pmc Domains of alpha-SNAP required for the stimulation of exocytosis and for N-ethylmalemide-sensitive fusion protein (NSF) binding and activation
    R J Barnard
    Physiological Laboratory, University of Liverpool, United Kingdom
    Mol Biol Cell 7:693-701. 1996
    ....
  21. pmc Localization and heterogeneity of agonist-induced changes in cytosolic calcium concentration in single bovine adrenal chromaffin cells from video imaging of fura-2
    A J O'Sullivan
    Physiological Laboratory, University of Liverpool, UK
    EMBO J 8:401-11. 1989
    ..A single large Ca2+ transient, with a component of the rise in [Ca2+]i occurring beneath the plasma membrane, may be the most potent signal for secretion...
  22. ncbi request reprint Analysis of regulated exocytosis in adrenal chromaffin cells: insights into NSF/SNAP/SNARE function
    R D Burgoyne
    Physiological Laboratory, University of Liverpool, UK
    Bioessays 20:328-35. 1998
    ..A major emphasis for the future will be analysis of the molecular physiology of regulated exocytosis to permit the assignment of functions to identified proteins in particular stages of the regulated exocytotic pathway...
  23. ncbi request reprint Fast exocytosis and endocytosis triggered by depolarisation in single adrenal chromaffin cells before rapid Ca2+ current run-down
    R D Burgoyne
    Physiological Laboratory, University of Liverpool, UK
    Pflugers Arch 430:213-9. 1995
    ..The fast endocytosis appears to have a higher threshold for activation than exocytosis, probably due to a higher Ca2+ requirement. Rapid membrane retrieval appears to occur via a clathrin-independent pathway in chromaffin cells...
  24. ncbi request reprint Activation of exocytosis by GTP analogues in adrenal chromaffin cells revealed by patch-clamp capacitance measurement
    R D Burgoyne
    Physiological Laboratory, Liverpool, UK
    FEBS Lett 344:139-42. 1994
    ..The magnitude of the response to XTP suggested that GTP analogues stimulate both exocytosis and recruitment of secretory granules...
  25. ncbi request reprint Neuronal Ca2+ sensor 1, the mammalian homologue of frequenin, is expressed in chromaffin and PC12 cells and regulates neurosecretion from dense-core granules
    B W McFerran
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 273:22768-72. 1998
    ..No effect of overexpression was seen on GH release because of Ca2+ in permeabilized cells suggesting that NCS-1 may have a regulatory but not direct role in neurosecretion...
  26. ncbi request reprint NSF and SNAP are present on adrenal chromaffin granules
    R D Burgoyne
    The Physiological Laboratory, University of Liverpool, UK
    FEBS Lett 414:349-52. 1997
    ..These results suggest that NSF and alpha-SNAP are associated with chromaffin granules and support the idea that they function prior to docking of the granules on the plasma membrane...
  27. pmc Exocytosis in adrenal chromaffin cells
    R D Burgoyne
    Department of Physiology, University of Liverpool, UK
    J Anat 183:309-14. 1993
    ..These include the peripheral plasma membrane protein annexin II and the cytosolic proteins, protein kinase C and 14-3-3 proteins (Exo1)...
  28. ncbi request reprint Histamine stimulates exocytosis in a sub-population of bovine adrenal chromaffin cells
    N Pender
    Physiological Laboratory, University of Liverpool, UK
    Neurosci Lett 144:207-10. 1992
    ..The results show that while up to 98% of the chromaffin cells in culture undergo exocytosis in response to nicotine, histamine stimulates exocytosis in only a sub-population of cells...
  29. ncbi request reprint Role of myristoylation in the intracellular targeting of neuronal calcium sensor (NCS) proteins
    D W O'Callaghan
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Biochem Soc Trans 31:963-5. 2003
    ..Additionally, the [Ca(2+)] dependency of the calcium-induced translocation of hippocalcin is investigated. The implications of the differential targeting of these proteins on calcium signal interpretation are considered...
  30. ncbi request reprint Cysteine string protein expression in mammary epithelial cells
    T L Gleave
    The Physiological Laboratory, University of Liverpool, UK
    Pflugers Arch 441:639-49. 2001
    ..Overexpression of Csp1 inhibits regulated exocytosis in other cell types but has no effect on constitutive GH release by KIM-2 cells. These results suggest that Csp does not have a major function in constitutive exocytosis...
  31. ncbi request reprint Calmodulin increases the initial rate of exocytosis in adrenal chromaffin cells
    A V Kibble
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK
    Pflugers Arch 431:464-6. 1996
    ....
  32. ncbi request reprint Regulation of exocytosis by protein kinase C
    A Morgan
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool, UK
    Biochem Soc Trans 33:1341-4. 2005
    ..In the present study, we review recent evidence suggesting that site-specific phosphorylation of SNAP-25 and Munc18 by PKC regulates distinct stages of exocytosis...
  33. ncbi request reprint Secretion of milk proteins
    R D Burgoyne
    The Physiological Laboratory, University of Liverpool, United Kingdom
    J Mammary Gland Biol Neoplasia 3:275-86. 1998
    ..The secretory biology of mammary epithelial cells has not been characterized extensively in recent years and, while some aspects are well understood, other key issues, which still remain to be resolved, have been highlighted...
  34. ncbi request reprint Identification of a novel cysteine string protein variant and expression of cysteine string proteins in non-neuronal cells
    L H Chamberlain
    Physiological Laboratory, University of Liverpool, United Kingdom
    J Biol Chem 271:7320-3. 1996
    ..We suggest that Csps may have a more general role in membrane traffic in non-neuronal as well as neuronal cells...
  35. pmc Regulated exocytosis
    R D Burgoyne
    Physiological Laboratory, University of Liverpool, U K
    Biochem J 293:305-16. 1993
  36. pmc Stimulation of NSF ATPase activity by alpha-SNAP is required for SNARE complex disassembly and exocytosis
    R J Barnard
    The Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, UK
    J Cell Biol 139:875-83. 1997
    ..These findings imply that alpha-SNAP stimulation of NSF ATPase activity may be required for 20S complex disassembly and for the alpha-SNAP stimulation of exocytosis...
  37. ncbi request reprint Calcium sensors in regulated exocytosis
    R D Burgoyne
    Physiological Laboratory, University of Liverpool, UK
    Cell Calcium 24:367-76. 1998
    ....
  38. ncbi request reprint Mechanisms of catecholamine secretion from adrenal chromaffin cells
    R D Burgoyne
    Physiological Laboratory, University of Liverpool, UK
    J Physiol Pharmacol 46:273-83. 1995
    ..The likely roles of these proteins in exocytosis are discussed in this review and the questions that remain for the understanding of the molecular basis of catecholamine release are highlighted...
  39. pmc Characterization of 14-3-3 proteins in adrenal chromaffin cells and demonstration of isoform-specific phospholipid binding
    D Roth
    Physiological Laboratory, University of Liverpool, U K
    Biochem J 301:305-10. 1994
    ..The Xenopus proteins lacks part of the extreme N-terminus, indicating that this domain is not essential for function in exocytosis...
  40. ncbi request reprint A comparison of bradykinin, angiotensin II and muscarinic stimulation of cultured bovine adrenal chromaffin cells
    A J O'Sullivan
    Physiological Laboratory, University of Liverpool, UK
    Biosci Rep 9:243-52. 1989
    ..Bradykinin also stimulates calcium entry, and this may increase the efficacy of the initial rise in [Ca2+]i. Treatment with pertussis toxin resulted in an enhancement of secretion in response to all of the secretagogues...
  41. pmc Differential distribution of beta-tubulin isotypes in cerebellum
    R D Burgoyne
    Physiological Laboratory, University of Liverpool, UK
    EMBO J 7:2311-9. 1988
    ..However, the strict subcellular segregation of other MAPs in brain may be determined by other factors...
  42. pmc Traffic of Kv4 K+ channels mediated by KChIP1 is via a novel post-ER vesicular pathway
    Burcu Hasdemir
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool L69 3BX, England, UK
    J Cell Biol 171:459-69. 2005
    ..When expressed in hippocampal neurons, KChIP1 co-distributed with dendritic Golgi outposts; therefore, the KChIP1 pathway could play an important role in local vesicular traffic in neurons...
  43. ncbi request reprint Syntaxin/Munc18 interactions in the late events during vesicle fusion and release in exocytosis
    Margaret E Graham
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 279:32751-60. 2004
    ..Modification of the Munc18-1/syntaxin 1A interaction would therefore be a key mechanism for the regulation of quantal size...
  44. ncbi request reprint Regulation of the fusion pore conductance during exocytosis by cyclin-dependent kinase 5
    Jeff W Barclay
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 279:41495-503. 2004
    ....
  45. ncbi request reprint Membrane traffic: controlling membrane fusion by modifying NSF
    Alan Morgan
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown St, P O Box 147, Liverpool L69 3BX, UK
    Curr Biol 14:R968-70. 2004
    ..Different cell types use distinct post-translational modifications of NSF for localized regulation of membrane fusion...
  46. pmc Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions
    Leonora F Ciufo
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool L69 3BX, United Kingdom
    Mol Biol Cell 16:470-82. 2005
    ..These results clarify the data on the function of SM proteins in mutant organisms and indicate that Munc18-1 controls multiple stages of exocytosis via both syntaxin-dependent and -independent protein interactions...
  47. ncbi request reprint Interaction of neuronal calcium sensor-1 and ADP-ribosylation factor 1 allows bidirectional control of phosphatidylinositol 4-kinase beta and trans-Golgi network-plasma membrane traffic
    Lee P Haynes
    Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown St, Liverpool L69 3BX, UK
    J Biol Chem 280:6047-54. 2005
    ..The NCS-1-ARF1 interaction provides evidence for functional cross-talk between Ca(2+)-dependent and ARF-dependent pathways in TGN to plasma membrane traffic...
  48. pmc A random mutagenesis approach to isolate dominant-negative yeast sec1 mutants reveals a functional role for domain 3a in yeast and mammalian Sec1/Munc18 proteins
    Alan Boyd
    Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool L69 3BX, United Kingdom
    Genetics 180:165-78. 2008
    ..Furthermore, this approach of screening for dominant-negative mutants in yeast may be useful for other conserved proteins, to identify functionally important domains in their mammalian homologs...
  49. ncbi request reprint Amisyn regulates exocytosis and fusion pore stability by both syntaxin-dependent and syntaxin-independent mechanisms
    John R L Constable
    Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool, L69 3BX, United Kingdom
    J Biol Chem 280:31615-23. 2005
    ..These results show that tomosyn and amisyn can negatively regulate exocytosis independently of syntaxin and also that amisyn can regulate the stability of the fusion pore...
  50. pmc High-affinity interaction of the N-terminal myristoylation motif of the neuronal calcium sensor protein hippocalcin with phosphatidylinositol 4,5-bisphosphate
    Dermott W O'Callaghan
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Biochem J 391:231-8. 2005
    ..The localization of NCS-1 and hippocalcin is likely to be determined, therefore, by their interaction with PtdIns(4,5)P2...
  51. ncbi request reprint Calcium-dependent regulation of exocytosis
    Jeff W Barclay
    Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Cell Calcium 38:343-53. 2005
    ....
  52. doi request reprint Unexpected tails of a Ca2+ sensor
    Lee P Haynes
    Nat Chem Biol 4:90-1. 2008
  53. ncbi request reprint Neuronal Ca2+-sensor proteins: multitalented regulators of neuronal function
    Robert D Burgoyne
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Trends Neurosci 27:203-9. 2004
    ..Recent work has established several physiological roles of these proteins, including diverse actions on gene expression, ion channel function, membrane traffic of ion channels and receptors, and the control of apoptosis...
  54. ncbi request reprint Identification of residues that determine the absence of a Ca(2+)/myristoyl switch in neuronal calcium sensor-1
    Dermott W O'Callaghan
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 279:14347-54. 2004
    ..We have determined a key role for residues within the motif EELTRK in NCS-1 in keeping the myristoyl group exposed and allowing the protein to be constitutively membrane-associated...
  55. ncbi request reprint Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator
    Hui Zhang
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 277:28948-58. 2002
    ....
  56. pmc Identification of Ca2+-dependent binding partners for the neuronal calcium sensor protein neurocalcin delta: interaction with actin, clathrin and tubulin
    Lenka Ivings
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool, L69 3BX, U K
    Biochem J 363:599-608. 2002
    ..These data suggest the possibility that neurocalcin delta functions in the control of clathrin-coated vesicle traffic...
  57. ncbi request reprint Complexin regulates the closure of the fusion pore during regulated vesicle exocytosis
    Deborah A Archer
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 277:18249-52. 2002
    ..The data are consistent with a function for complexin in stabilizing an intermediate of the SNARE complex to allow kiss-and-run recycling of the exocytosed vesicle...
  58. ncbi request reprint Molecular analysis of SNAP-25 function in exocytosis
    Margaret E Graham
    The Physiological Laboratory, University of Liverpool, Liverpool, L69 3BX, United Kingdom
    Ann N Y Acad Sci 971:210-21. 2002
    ..The data suggest an important role of the cysteines, but not the 0 layer glutamines, in triggered exocytosis...
  59. ncbi request reprint Phosphorylation of Munc18 by protein kinase C regulates the kinetics of exocytosis
    Jeff W Barclay
    Physiological Laboratory, University of Liverpool, Crown Street, United Kingdom
    J Biol Chem 278:10538-45. 2003
    ..Phosphorylation of Munc18 by PKC may provide a mechanism for the control of exocytosis and thereby synaptic plasticity...
  60. ncbi request reprint Synaptotagmin interaction with the syntaxin/SNAP-25 dimer is mediated by an evolutionarily conserved motif and is sensitive to inositol hexakisphosphate
    Colin Rickman
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 279:12574-9. 2004
    ..Our results shed new light on the functional importance of the conserved polybasic synaptotagmin motif, suggesting that synaptotagmin interacts with the t-SNARE dimer to up-regulate the probability of SNARE-mediated membrane fusion...
  61. ncbi request reprint IL1 receptor accessory protein like, a protein involved in X-linked mental retardation, interacts with Neuronal Calcium Sensor-1 and regulates exocytosis
    Nadia Bahi
    Institut Cochin, INSERM Unité 567, CNRS UMR 8104, Universite Paris V, CHU Cochin, 24 rue du Faubourg Saint Jacques, 75 014 Paris, France
    Hum Mol Genet 12:1415-25. 2003
    ..Taken together, our data suggest that IL1RAPL may regulate calcium-dependent exocytosis and provide insight into the understanding of physiopathological mechanisms underlying cognitive impairment resulting from IL1RAPL dysfunction...
  62. ncbi request reprint Tying everything together: the multiple roles of cysteine string protein (CSP) in regulated exocytosis
    Gareth J O Evans
    The Physiological Laboratory, Department of Physiology, University of Liverpool, Crown St, Liverpool, L69 3BX, UK
    Traffic 4:653-9. 2003
    ..In addition, we provide an update on the mammalian isoforms of cysteine string protein following the recent discovery of two novel cysteine string proteins...
  63. ncbi request reprint Calcium-binding protein 1 is an inhibitor of agonist-evoked, inositol 1,4,5-trisphosphate-mediated calcium signaling
    Lee P Haynes
    The Physiological Laboratory, Crown Street, University of Liverpool, Liverpool L69 3BX, UK
    J Biol Chem 279:547-55. 2004
    ..Together, these data suggest that L-CaBP1 is able to specifically regulate InsP3 receptor-mediated alterations in [Ca2+]i during agonist stimulation...
  64. ncbi request reprint Differential use of myristoyl groups on neuronal calcium sensor proteins as a determinant of spatio-temporal aspects of Ca2+ signal transduction
    Dermott W O'Callaghan
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 277:14227-37. 2002
    ..These results demonstrate that closely related Ca(2+) sensor proteins use their myristoyl groups in distinct ways in vivo in a manner that will determine the time course of Ca(2+) signal transduction...
  65. pmc Activation of the store-operated calcium current ICRAC can be dissociated from regulated exocytosis in rat basophilic leukaemia (RBL-1) cells
    Daniel Bakowski
    Laboratory of Molecular and Cellular Signalling, Department of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, UK
    J Physiol 553:387-93. 2003
    ..Activation of ICRAC can therefore be clearly dissociated from an exocytotic mechanism, a finding that is not easily reconcilable with the vesicular fusion model...
  66. ncbi request reprint Residues within the myristoylation motif determine intracellular targeting of the neuronal Ca2+ sensor protein KChIP1 to post-ER transport vesicles and traffic of Kv4 K+ channels
    Dermott W O'Callaghan
    The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    J Cell Sci 116:4833-45. 2003
    ..We suggest that KChIP1 is targeted by its myristoylation motif to post-ER transport vesicles where it could interact with and regulate the traffic of Kv4 channels to the plasma membrane under the influence of localised Ca2+ signals...
  67. pmc Dynamics and calcium sensitivity of the Ca2+/myristoyl switch protein hippocalcin in living cells
    Dermott W O'Callaghan
    Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, England, UK
    J Cell Biol 163:715-21. 2003
    ..The data show that hippocalcin is a sensitive Ca2+ sensor capable of responding to increases in intracellular Ca2+ concentration over the narrow dynamic range of 200-800 nM free Ca2+...
  68. pmc Specific effects of KChIP3/calsenilin/DREAM, but not KChIPs 1, 2 and 4, on calcium signalling and regulated secretion in PC12 cells
    Neil Venn
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Biochem J 413:71-80. 2008
    ..These results reveal a new role for KChIP3 in the regulation of Ca2+-regulated secretion and also suggest that the functions of each of the KChIPs may be more specialized than previously appreciated...
  69. doi request reprint S-nitrosylation of syntaxin 1 at Cys(145) is a regulatory switch controlling Munc18-1 binding
    Zoë J Palmer
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown St, Liverpool L69 3BX, U K
    Biochem J 413:479-91. 2008
    ..Thus S-nitrosylation of Cys(145) may be a molecular switch to disrupt Munc18-1 binding to the closed conformation of syntaxin 1a, thereby facilitating its engagement with the membrane fusion machinery...
  70. doi request reprint The Rab27 effector Rabphilin, unlike Granuphilin and Noc2, rapidly exchanges between secretory granules and cytosol in PC12 cells
    Mark T W Handley
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool L693BX, UK
    Biochem Biophys Res Commun 373:275-81. 2008
    ....
  71. ncbi request reprint Splitting the quantum: regulation of quantal release during vesicle fusion
    Robert D Burgoyne
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool, UK L69 3BX
    Trends Neurosci 25:176-8. 2002
    ....
  72. pmc Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size
    Margaret E Graham
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    Proc Natl Acad Sci U S A 99:7124-9. 2002
    ..The effects of phorbol ester treatment were not prevented by expression of the amphiphysin SH3 domain or by GTP gamma S suggesting the existence of alternative dynamin-independent process underlying fast kiss-and-run exocytosis...
  73. ncbi request reprint Secretory granule exocytosis
    Robert D Burgoyne
    The Physiological Laboratory, University of Liverpool, United Kingdom
    Physiol Rev 83:581-632. 2003
    ....
  74. pmc Neuronal calcium sensor proteins are unable to modulate NFAT activation in mammalian cells
    Daniel J Fitzgerald
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK
    Biochim Biophys Acta 1780:240-8. 2008
    ....
  75. ncbi request reprint A gain-of-function mutant of Munc18-1 stimulates secretory granule recruitment and exocytosis and reveals a direct interaction of Munc18-1 with Rab3
    Margaret E Graham
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    Biochem J 409:407-16. 2008
    ....
  76. ncbi request reprint The Rab-binding protein Noc2 is associated with insulin-containing secretory granules and is essential for pancreatic beta-cell exocytosis
    Séverine Cheviet
    Institut de Biologie Cellulaire et de Morphologie, University of Lausanne, Switzerland
    Mol Endocrinol 18:117-26. 2004
    ..These data suggest that Noc2 is involved in the recruitment of secretory granules at the plasma membrane possibly via the interaction with Munc13...
  77. ncbi request reprint Sense and sensibility in the regulation of voltage-gated Ca(2+) channels
    Jamie L Weiss
    The Physiological Laboratory, University of Liverpool, UK
    Trends Neurosci 25:489-91. 2002
    ..These have complex effects on channel gating, and data suggest that the actions of multiple Ca(2+) sensors are important for the fine-tuning of Ca(2+) channel activity...
  78. pmc Conserved prefusion protein assembly in regulated exocytosis
    Colin Rickman
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom
    Mol Biol Cell 17:283-94. 2006
    ....
  79. ncbi request reprint Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta
    Lee P Haynes
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool, UK
    Proteomics 6:1822-32. 2006
    ..These findings provide new clues to the functional roles of the neuronal calcium sensor proteins...
  80. pmc Localized Ca2+ uncaging reveals polarized distribution of Ca2+-sensitive Ca2+ release sites: mechanism of unidirectional Ca2+ waves
    Michael C Ashby
    Medical Research Council Secretory Control Research Group, The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, UK
    J Cell Biol 158:283-92. 2002
    ..CICR is the major process responsible for global Ca2+ transients, and intracellular variations in sensitivity to CICR predetermine the activation pattern of Ca2+ waves...
  81. pmc Differential dynamics of Rab3A and Rab27A on secretory granules
    Mark T W Handley
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK
    J Cell Sci 120:973-84. 2007
    ....
  82. ncbi request reprint Membrane trafficking: three steps to fusion
    Robert D Burgoyne
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool, UK
    Curr Biol 17:R255-8. 2007
    ..Recent work has established that Munc18-1 directly stimulates fusion and possibly uses all three modes of SNARE interaction...
  83. pmc Specificity, promiscuity and localization of ARF protein interactions with NCS-1 and phosphatidylinositol-4 kinase-III beta
    Lee P Haynes
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool, L69 3BX, UK
    Traffic 8:1080-92. 2007
    ..Consistent with these latter findings ARF1 but not ARF5 or 6 enhanced the stimulatory effect of PI4Kbeta on regulated exocytosis, suggesting a specific role for class-I ARFs in the regulation of PI4Kbeta...
  84. pmc Protein kinase B/Akt is a novel cysteine string protein kinase that regulates exocytosis release kinetics and quantal size
    Gareth J O Evans
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool L69 3BX, United Kingdom
    J Biol Chem 281:1564-72. 2006
    ..Taken together, these findings reveal a novel role for Akt phosphorylation in regulating the late stages of exocytosis and suggest that this is achieved via the phosphorylation of CSP on serine 10...