Research Topics
| Steven D BuckinghamSummaryAffiliation: University of Oxford Country: UK Publications
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Detail Information
Publications
Bicuculline-insensitive GABA-gated Cl- channels in the larval nervous system of the moth Manduca sextaDavid B Sattelle
MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
Invert Neurosci 5:37-43. 2003..Thus, bicuculline-insensitive GABA-gated Cl(-) channels are present in the larval nervous system of M. sexta...- Structure and function of two-pore-domain K+ channels: contributions from genetic model organismsSteven D Buckingham
MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
Trends Pharmacol Sci 26:361-7. 2005..In this article, we review the contributions of some of these genetic model organisms to recent advances in our knowledge of two-pore-domain K+ channels... - Replacement of asparagine with arginine at the extracellular end of the second transmembrane (M2) region of insect GABA receptors increases sensitivity to penicillin GAlastair M Hosie
Department of Pharmacology, University College, Gower St, London WC1E 6BT, UK
Invert Neurosci 6:75-9. 2006..The A301S mutation that confers resistance to dieldrin did not significantly affect the antagonism by PCG... - Functional genomics: applying calcium imaging and RNA interference to Drosophila cell lines to identify new roles for gene productsSteven D Buckingham
MRC Functional Genetics Unit, Department of Physiology, Anatomy and Genetics Le Gros Clark Building, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK
Invert Neurosci 6:141-4. 2006..This technical note describes the application of this approach to the Drosophila S2 cell line and its derivatives... 
Splice-variant- and stage-specific RNA editing of the Drosophila GABA receptor modulates agonist potencyAndrew K Jones
Medical Research Council, Functional Genomics Unit, University of Oxford, Oxford OX1 3QX, United Kingdom
J Neurosci 29:4287-92. 2009..These results provide insights into how the rich diversity of signaling necessary for complex brain function can be achieved by relatively few genes...- Alternative splicing of the Anopheles gambiae nicotinic acetylcholine receptor, Agamalphabeta9, generates both alpha and beta subunitsAndrew K Jones
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
Invert Neurosci 9:77-84. 2009..Our findings further characterise a potential target to control the malaria mosquito as well as provide insights into the diversification of nAChRs... - Comparative pharmacology and computational modelling yield insights into allosteric modulation of human alpha7 nicotinic acetylcholine receptorsDavid B Sattelle
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK
Biochem Pharmacol 78:836-43. 2009..The new pharmacological findings and computational approaches being developed may inform the design of novel PAM drugs targeting major neurological disorders... 
Nicotinic acetylcholine receptor signalling: roles in Alzheimer's disease and amyloid neuroprotectionSteven D Buckingham
Medical Research Council Functional Genomics Unit, Department of Physiology Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, UK, OX1 3QX
Pharmacol Rev 61:39-61. 2009..We propose that understanding the differential activation of these pathways by nicotine and/or Abeta(1-42) may offer the prospect of new routes to therapy for AD...- Nicotinic acetylcholine receptors as drug/chemical targets, contributions from comparative genomics, forward and reverse geneticsDavid B Sattelle
University of Oxford, UK
SEB Exp Biol Ser 58:93-113. 2007 - The Abeta1-42M35C mutated amyloid peptide Abeta1-42 and the 25-35 fragment fail to mimic the subtype-specificity of actions on recombinant human nicotinic acetylcholine receptors (alpha7, alpha4beta2, alpha3beta4)Luanda J Pym
MRC Functional Genetics Unit, Department of Physiology, Anatomy and Genetics, Oxford University, South Parks Road, Oxford OX1 3QX, United Kingdom
Neurosci Lett 427:28-33. 2007.... - Allosteric modulation by benzodiazepines of GABA-gated chloride channels of an identified insect motor neuroneSteven D Buckingham
MRC Functional Genomics Unit, Department of Physiology Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
Invert Neurosci 9:85-9. 2009.... - A Cys-loop mutation in the Caenorhabditis elegans nicotinic receptor subunit UNC-63 impairs but does not abolish channel functionAndrew K Jones
Medical Research Council Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, United Kingdom
J Biol Chem 286:2550-8. 2011..The C. elegans unc-63(x26) mutant may therefore offer a useful model to assist in the development of therapies for syndromes produced by altered function of human nAChRs... - Neonicotinoid insecticides display partial and super agonist actions on native insect nicotinic acetylcholine receptorsLaurence A Brown
Department of Physiology, Anatomy and Genetics, MRC Functional Genetics Unit, Le Gros Clark Building, University of Oxford, South Parks Road, Oxford, UK
J Neurochem 99:608-15. 2006..Cell-attached recordings indicate that super agonism results from more frequent openings at the largest (63.5 pS) conductance state observed... - A novel Caenorhabditis elegans allele, smn-1(cb131), mimicking a mild form of spinal muscular atrophy, provides a convenient drug screening platform highlighting new and pre-approved compoundsJames N Sleigh
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
Hum Mol Genet 20:245-60. 2011..4-aminopyridine, gaboxadol hydrochloride and N-acetylneuraminic acid all rescued at least one aspect of smn-1 phenotypic dysfunction. These findings may assist in accelerating the development of drugs for the treatment of SMA... - Proteins interacting with nicotinic acetylcholine receptors: expanding functional and therapeutic horizonsAndrew K Jones
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3QX, UK
Trends Pharmacol Sci 31:455-62. 2010..Mutations giving rise to disease states highlight the importance of these interacting proteins. Here, we consider their potential as future therapeutic targets for treating diseases associated with altered nAChR function... - Fast, automated measurement of nematode swimming (thrashing) without morphometrySteven D Buckingham
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK
BMC Neurosci 10:84. 2009..Our method uses principal components analysis to remove the background, followed by computation of a covariance matrix of the remaining image frames from which the interval between statistically-similar frames is estimated... - Chemistry-to-gene screens in Caenorhabditis elegansAndrew K Jones
MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
Nat Rev Drug Discov 4:321-30. 2005..Here, we show that chemistry-to-gene screens using this animal model can define targets of antiparasitic drugs, identify novel candidate drug targets and contribute to the discovery of new drugs for treating human diseases... - Strategies for automated analysis of C. elegans locomotionSteven D Buckingham
MRC Functional Genomics Unit, Department of Physiology Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
Invert Neurosci 8:121-31. 2008..Nonetheless, recent advances have shown that drug actions on large numbers of worms can be tracked, raising hopes that high-throughput behavioural screens may soon be available... - RNA interference: from model organisms towards therapy for neural and neuromuscular disordersSteven D Buckingham
MRC Functional Genetics Unit, University of Oxford, Oxford, UK
Hum Mol Genet 13:R275-88. 2004..Here, we review examples of the application of RNAi to worm, fly and mouse models of such diseases aimed at understanding their pathophysiology and we address problems to be solved in developing RNAi-based therapies... - Computer simulations of high-pass filtering in zebrafish larval muscle fibresSteven D Buckingham
MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK
J Exp Biol 208:3055-63. 2005..Although a contribution of other currents to produce once-only firing has not been excluded, this model suggests that the properties of the sodium current are sufficient to account for once-only firing... - Data mining for protein-protein interactions in invertebrate model organismsSteven D Buckingham
MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, UK
Invert Neurosci 5:183-7. 2005.... - A7DB: a relational database for mutational, physiological and pharmacological data related to the alpha7 nicotinic acetylcholine receptorSteven D Buckingham
Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
BMC Neurosci 6:2. 2005..The growing amount of structural, pharmacological and physiological data for these receptors indicates the need for a dedicated and accurate database to provide a means to access this information in a coherent manner... - Action potential shape change in an electrically coupled network during propagation: a computer simulationSteven D Buckingham
Bamfield Marine Sciences Centre, Bamfield, BC, Canada
Invert Neurosci 8:83-9. 2008..These data suggest that the spike shape changes may be due not only to potassium channel inactivation, but also to the passive properties of the membrane...