Steven D Buckingham

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. ncbi request reprint Bicuculline-insensitive GABA-gated Cl- channels in the larval nervous system of the moth Manduca sexta
    David B Sattelle
    MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Invert Neurosci 5:37-43. 2003
  2. ncbi request reprint Structure and function of two-pore-domain K+ channels: contributions from genetic model organisms
    Steven D Buckingham
    MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Trends Pharmacol Sci 26:361-7. 2005
  3. ncbi request reprint Replacement of asparagine with arginine at the extracellular end of the second transmembrane (M2) region of insect GABA receptors increases sensitivity to penicillin G
    Alastair M Hosie
    Department of Pharmacology, University College, Gower St, London WC1E 6BT, UK
    Invert Neurosci 6:75-9. 2006
  4. ncbi request reprint Functional genomics: applying calcium imaging and RNA interference to Drosophila cell lines to identify new roles for gene products
    Steven D Buckingham
    MRC Functional Genetics Unit, Department of Physiology, Anatomy and Genetics Le Gros Clark Building, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK
    Invert Neurosci 6:141-4. 2006
  5. doi request reprint Splice-variant- and stage-specific RNA editing of the Drosophila GABA receptor modulates agonist potency
    Andrew K Jones
    Medical Research Council, Functional Genomics Unit, University of Oxford, Oxford OX1 3QX, United Kingdom
    J Neurosci 29:4287-92. 2009
  6. doi request reprint Alternative splicing of the Anopheles gambiae nicotinic acetylcholine receptor, Agamalphabeta9, generates both alpha and beta subunits
    Andrew K Jones
    MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Invert Neurosci 9:77-84. 2009
  7. doi request reprint Comparative pharmacology and computational modelling yield insights into allosteric modulation of human alpha7 nicotinic acetylcholine receptors
    David B Sattelle
    MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK
    Biochem Pharmacol 78:836-43. 2009
  8. pmc Nicotinic acetylcholine receptor signalling: roles in Alzheimer's disease and amyloid neuroprotection
    Steven D Buckingham
    Medical Research Council Functional Genomics Unit, Department of Physiology Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, UK, OX1 3QX
    Pharmacol Rev 61:39-61. 2009
  9. ncbi request reprint Nicotinic acetylcholine receptors as drug/chemical targets, contributions from comparative genomics, forward and reverse genetics
    David B Sattelle
    University of Oxford, UK
    SEB Exp Biol Ser 58:93-113. 2007
  10. ncbi request reprint The Abeta1-42M35C mutated amyloid peptide Abeta1-42 and the 25-35 fragment fail to mimic the subtype-specificity of actions on recombinant human nicotinic acetylcholine receptors (alpha7, alpha4beta2, alpha3beta4)
    Luanda J Pym
    MRC Functional Genetics Unit, Department of Physiology, Anatomy and Genetics, Oxford University, South Parks Road, Oxford OX1 3QX, United Kingdom
    Neurosci Lett 427:28-33. 2007

Collaborators

Detail Information

Publications23

  1. ncbi request reprint Bicuculline-insensitive GABA-gated Cl- channels in the larval nervous system of the moth Manduca sexta
    David B Sattelle
    MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Invert Neurosci 5:37-43. 2003
    ..Thus, bicuculline-insensitive GABA-gated Cl(-) channels are present in the larval nervous system of M. sexta...
  2. ncbi request reprint Structure and function of two-pore-domain K+ channels: contributions from genetic model organisms
    Steven D Buckingham
    MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Trends Pharmacol Sci 26:361-7. 2005
    ..In this article, we review the contributions of some of these genetic model organisms to recent advances in our knowledge of two-pore-domain K+ channels...
  3. ncbi request reprint Replacement of asparagine with arginine at the extracellular end of the second transmembrane (M2) region of insect GABA receptors increases sensitivity to penicillin G
    Alastair M Hosie
    Department of Pharmacology, University College, Gower St, London WC1E 6BT, UK
    Invert Neurosci 6:75-9. 2006
    ..The A301S mutation that confers resistance to dieldrin did not significantly affect the antagonism by PCG...
  4. ncbi request reprint Functional genomics: applying calcium imaging and RNA interference to Drosophila cell lines to identify new roles for gene products
    Steven D Buckingham
    MRC Functional Genetics Unit, Department of Physiology, Anatomy and Genetics Le Gros Clark Building, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK
    Invert Neurosci 6:141-4. 2006
    ..This technical note describes the application of this approach to the Drosophila S2 cell line and its derivatives...
  5. doi request reprint Splice-variant- and stage-specific RNA editing of the Drosophila GABA receptor modulates agonist potency
    Andrew K Jones
    Medical Research Council, Functional Genomics Unit, University of Oxford, Oxford OX1 3QX, United Kingdom
    J Neurosci 29:4287-92. 2009
    ..These results provide insights into how the rich diversity of signaling necessary for complex brain function can be achieved by relatively few genes...
  6. doi request reprint Alternative splicing of the Anopheles gambiae nicotinic acetylcholine receptor, Agamalphabeta9, generates both alpha and beta subunits
    Andrew K Jones
    MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Invert Neurosci 9:77-84. 2009
    ..Our findings further characterise a potential target to control the malaria mosquito as well as provide insights into the diversification of nAChRs...
  7. doi request reprint Comparative pharmacology and computational modelling yield insights into allosteric modulation of human alpha7 nicotinic acetylcholine receptors
    David B Sattelle
    MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK
    Biochem Pharmacol 78:836-43. 2009
    ..The new pharmacological findings and computational approaches being developed may inform the design of novel PAM drugs targeting major neurological disorders...
  8. pmc Nicotinic acetylcholine receptor signalling: roles in Alzheimer's disease and amyloid neuroprotection
    Steven D Buckingham
    Medical Research Council Functional Genomics Unit, Department of Physiology Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, UK, OX1 3QX
    Pharmacol Rev 61:39-61. 2009
    ..We propose that understanding the differential activation of these pathways by nicotine and/or Abeta(1-42) may offer the prospect of new routes to therapy for AD...
  9. ncbi request reprint Nicotinic acetylcholine receptors as drug/chemical targets, contributions from comparative genomics, forward and reverse genetics
    David B Sattelle
    University of Oxford, UK
    SEB Exp Biol Ser 58:93-113. 2007
  10. ncbi request reprint The Abeta1-42M35C mutated amyloid peptide Abeta1-42 and the 25-35 fragment fail to mimic the subtype-specificity of actions on recombinant human nicotinic acetylcholine receptors (alpha7, alpha4beta2, alpha3beta4)
    Luanda J Pym
    MRC Functional Genetics Unit, Department of Physiology, Anatomy and Genetics, Oxford University, South Parks Road, Oxford OX1 3QX, United Kingdom
    Neurosci Lett 427:28-33. 2007
    ....
  11. doi request reprint Allosteric modulation by benzodiazepines of GABA-gated chloride channels of an identified insect motor neurone
    Steven D Buckingham
    MRC Functional Genomics Unit, Department of Physiology Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Invert Neurosci 9:85-9. 2009
    ....
  12. pmc A Cys-loop mutation in the Caenorhabditis elegans nicotinic receptor subunit UNC-63 impairs but does not abolish channel function
    Andrew K Jones
    Medical Research Council Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, United Kingdom
    J Biol Chem 286:2550-8. 2011
    ..The C. elegans unc-63(x26) mutant may therefore offer a useful model to assist in the development of therapies for syndromes produced by altered function of human nAChRs...
  13. ncbi request reprint Neonicotinoid insecticides display partial and super agonist actions on native insect nicotinic acetylcholine receptors
    Laurence A Brown
    Department of Physiology, Anatomy and Genetics, MRC Functional Genetics Unit, Le Gros Clark Building, University of Oxford, South Parks Road, Oxford, UK
    J Neurochem 99:608-15. 2006
    ..Cell-attached recordings indicate that super agonism results from more frequent openings at the largest (63.5 pS) conductance state observed...
  14. doi request reprint A novel Caenorhabditis elegans allele, smn-1(cb131), mimicking a mild form of spinal muscular atrophy, provides a convenient drug screening platform highlighting new and pre-approved compounds
    James N Sleigh
    MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
    Hum Mol Genet 20:245-60. 2011
    ..4-aminopyridine, gaboxadol hydrochloride and N-acetylneuraminic acid all rescued at least one aspect of smn-1 phenotypic dysfunction. These findings may assist in accelerating the development of drugs for the treatment of SMA...
  15. doi request reprint Proteins interacting with nicotinic acetylcholine receptors: expanding functional and therapeutic horizons
    Andrew K Jones
    MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3QX, UK
    Trends Pharmacol Sci 31:455-62. 2010
    ..Mutations giving rise to disease states highlight the importance of these interacting proteins. Here, we consider their potential as future therapeutic targets for treating diseases associated with altered nAChR function...
  16. pmc Fast, automated measurement of nematode swimming (thrashing) without morphometry
    Steven D Buckingham
    MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK
    BMC Neurosci 10:84. 2009
    ..Our method uses principal components analysis to remove the background, followed by computation of a covariance matrix of the remaining image frames from which the interval between statistically-similar frames is estimated...
  17. ncbi request reprint Chemistry-to-gene screens in Caenorhabditis elegans
    Andrew K Jones
    MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Nat Rev Drug Discov 4:321-30. 2005
    ..Here, we show that chemistry-to-gene screens using this animal model can define targets of antiparasitic drugs, identify novel candidate drug targets and contribute to the discovery of new drugs for treating human diseases...
  18. doi request reprint Strategies for automated analysis of C. elegans locomotion
    Steven D Buckingham
    MRC Functional Genomics Unit, Department of Physiology Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
    Invert Neurosci 8:121-31. 2008
    ..Nonetheless, recent advances have shown that drug actions on large numbers of worms can be tracked, raising hopes that high-throughput behavioural screens may soon be available...
  19. ncbi request reprint RNA interference: from model organisms towards therapy for neural and neuromuscular disorders
    Steven D Buckingham
    MRC Functional Genetics Unit, University of Oxford, Oxford, UK
    Hum Mol Genet 13:R275-88. 2004
    ..Here, we review examples of the application of RNAi to worm, fly and mouse models of such diseases aimed at understanding their pathophysiology and we address problems to be solved in developing RNAi-based therapies...
  20. ncbi request reprint Computer simulations of high-pass filtering in zebrafish larval muscle fibres
    Steven D Buckingham
    MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK
    J Exp Biol 208:3055-63. 2005
    ..Although a contribution of other currents to produce once-only firing has not been excluded, this model suggests that the properties of the sodium current are sufficient to account for once-only firing...
  21. ncbi request reprint Data mining for protein-protein interactions in invertebrate model organisms
    Steven D Buckingham
    MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, UK
    Invert Neurosci 5:183-7. 2005
    ....
  22. pmc A7DB: a relational database for mutational, physiological and pharmacological data related to the alpha7 nicotinic acetylcholine receptor
    Steven D Buckingham
    Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    BMC Neurosci 6:2. 2005
    ..The growing amount of structural, pharmacological and physiological data for these receptors indicates the need for a dedicated and accurate database to provide a means to access this information in a coherent manner...
  23. doi request reprint Action potential shape change in an electrically coupled network during propagation: a computer simulation
    Steven D Buckingham
    Bamfield Marine Sciences Centre, Bamfield, BC, Canada
    Invert Neurosci 8:83-9. 2008
    ..These data suggest that the spike shape changes may be due not only to potassium channel inactivation, but also to the passive properties of the membrane...