David Brough

Summary

Affiliation: University of Manchester
Country: UK

Publications

  1. pmc Inhibition of calpain blocks the phagosomal escape of Listeria monocytogenes
    Gloria Lopez-Castejon
    Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    PLoS ONE 7:e35936. 2012
  2. pmc The dynamics and mechanisms of interleukin-1alpha and beta nuclear import
    Nadia M Luheshi
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, UK
    Traffic 10:16-25. 2009
  3. pmc Understanding the mechanism of IL-1β secretion
    Gloria Lopez-Castejon
    Faculty of Life Sciences, University of Manchester, AV Hill Building, Oxford Road, Manchester M13 9PT, UK
    Cytokine Growth Factor Rev 22:189-95. 2011
  4. pmc Deubiquitinases regulate the activity of caspase-1 and interleukin-1β secretion via assembly of the inflammasome
    Gloria Lopez-Castejon
    AV Hill Building, Faculty of Life Sciences, University of Manchester Manchester, M13 9PT, United Kingdom
    J Biol Chem 288:2721-33. 2013
  5. pmc Sphingosine regulates the NLRP3-inflammasome and IL-1β release from macrophages
    Nadia M Luheshi
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    Eur J Immunol 42:716-25. 2012
  6. pmc Caspase-1: is IL-1 just the tip of the ICEberg?
    A Denes
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    Cell Death Dis 3:e338. 2012
  7. ncbi request reprint The structural integrity of the endoplasmic reticulum, and its possible regulation by inositol 1,3,4,5-tetrakisphosphate
    David Brough
    Department of Pharmacology, Cambridge, UK
    Cell Calcium 38:153-9. 2005
  8. ncbi request reprint Caspase-1-dependent processing of pro-interleukin-1beta is cytosolic and precedes cell death
    David Brough
    Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester, M13 9PT, UK
    J Cell Sci 120:772-81. 2007
  9. pmc Pannexin-1-dependent caspase-1 activation and secretion of IL-1beta is regulated by zinc
    David Brough
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    Eur J Immunol 39:352-8. 2009
  10. ncbi request reprint Interleukin-18 induces expression and release of cytokines from murine glial cells: interactions with interleukin-1 beta
    Rachel D Wheeler
    School of Biological Sciences, University of Manchester, Oxford Road, Manchester, UK
    J Neurochem 85:1412-20. 2003

Collaborators

Detail Information

Publications31

  1. pmc Inhibition of calpain blocks the phagosomal escape of Listeria monocytogenes
    Gloria Lopez-Castejon
    Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    PLoS ONE 7:e35936. 2012
    ..These data indicate that L. monocytogenes co-opts host calpain to facilitate its escape from the phagosome, and more generally, that calpain may represent a cellular Achilles heel exploited by pathogens...
  2. pmc The dynamics and mechanisms of interleukin-1alpha and beta nuclear import
    Nadia M Luheshi
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, UK
    Traffic 10:16-25. 2009
    ..These data provide valuable insights into the dynamic regulation of intracellular cytokine trafficking...
  3. pmc Understanding the mechanism of IL-1β secretion
    Gloria Lopez-Castejon
    Faculty of Life Sciences, University of Manchester, AV Hill Building, Oxford Road, Manchester M13 9PT, UK
    Cytokine Growth Factor Rev 22:189-95. 2011
    ..Here we summarise these observations and propose that secretion of IL-1β occurs on a continuum, dependent upon stimulus strength and the extracellular IL-1β requirement...
  4. pmc Deubiquitinases regulate the activity of caspase-1 and interleukin-1β secretion via assembly of the inflammasome
    Gloria Lopez-Castejon
    AV Hill Building, Faculty of Life Sciences, University of Manchester Manchester, M13 9PT, United Kingdom
    J Biol Chem 288:2721-33. 2013
    ..These data reveal the requirement for DUB activity in a key reaction of the innate immune response and highlight the therapeutic potential of DUB inhibitors for chronic auto-inflammatory diseases...
  5. pmc Sphingosine regulates the NLRP3-inflammasome and IL-1β release from macrophages
    Nadia M Luheshi
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    Eur J Immunol 42:716-25. 2012
    ..Thus, we propose Sph as a new DAMP, and that a serine/threonine phosphatase (PP1/PP2A)-dependent signal is central to the endogenous host mechanism through which diverse stimuli regulate inflammasome activation...
  6. pmc Caspase-1: is IL-1 just the tip of the ICEberg?
    A Denes
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    Cell Death Dis 3:e338. 2012
    ..We attempt here to summarise the evidence for caspase-1 as a cell death enzyme and propose that, in addition to the processing of IL-1β, caspase-1 has an important and a conserved role as a cell death protease...
  7. ncbi request reprint The structural integrity of the endoplasmic reticulum, and its possible regulation by inositol 1,3,4,5-tetrakisphosphate
    David Brough
    Department of Pharmacology, Cambridge, UK
    Cell Calcium 38:153-9. 2005
    ..However, we were subsequently unable to detect by FRAP any significant effect of Ins(1,3,4,5)P(4) on ER integrity...
  8. ncbi request reprint Caspase-1-dependent processing of pro-interleukin-1beta is cytosolic and precedes cell death
    David Brough
    Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester, M13 9PT, UK
    J Cell Sci 120:772-81. 2007
    ..We propose that, in macrophages, ATP-induced interleukin-1beta processing occurs in the cytosol by a mechanism that resembles pyroptosis...
  9. pmc Pannexin-1-dependent caspase-1 activation and secretion of IL-1beta is regulated by zinc
    David Brough
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    Eur J Immunol 39:352-8. 2009
    ..These data provide new insights into the mechanisms of caspase-1 activation and how zinc metabolism contributes to inflammatory mechanisms...
  10. ncbi request reprint Interleukin-18 induces expression and release of cytokines from murine glial cells: interactions with interleukin-1 beta
    Rachel D Wheeler
    School of Biological Sciences, University of Manchester, Oxford Road, Manchester, UK
    J Neurochem 85:1412-20. 2003
    ..These data suggest that IL-18 may contribute to inflammatory responses in the brain, and demonstrate that, in spite of several common features, IL-18 and IL-1 beta differ in their regulation and actions...
  11. pmc Acidosis drives damage-associated molecular pattern (DAMP)-induced interleukin-1 secretion via a caspase-1-independent pathway
    Michelle E Edye
    From the Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 288:30485-94. 2013
    ..Therapeutic strategies directed to the inhibition of IL-1β processing should therefore consider alternative processing of IL-1β in addition to caspase-1-dependent processing. ..
  12. ncbi request reprint Purinergic (P2X7) receptor activation of microglia induces cell death via an interleukin-1-independent mechanism
    David Brough
    Division of Neuroscience, School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Mol Cell Neurosci 19:272-80. 2002
    ..Microglia from mice lacking the IL-1beta gene demonstrated a profile of death identical to that of wild-type microglia in response to LPS and ATP. Thus, IL-1beta is not required for P2X7 receptor-stimulated microglial death...
  13. ncbi request reprint Ca2+ stores and Ca2+ entry differentially contribute to the release of IL-1 beta and IL-1 alpha from murine macrophages
    David Brough
    School of Biological Sciences, University of Manchester, Manchester, United Kingdom
    J Immunol 170:3029-36. 2003
    ..The identification of these mechanisms provides insight into the mechanism of IL-1 secretion, and may lead to the identification of targets for the therapeutic modulation of IL-1 action in inflammation...
  14. doi request reprint Neuronal injury induces the release of pro-interleukin-1beta from activated microglia in vitro
    Penglian Wang
    Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK
    Brain Res 1236:1-7. 2008
    ....
  15. ncbi request reprint Priming of macrophages with lipopolysaccharide potentiates P2X7-mediated cell death via a caspase-1-dependent mechanism, independently of cytokine production
    Rosalind A Le Feuvre
    School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 277:3210-8. 2002
    ....
  16. pmc Microglia and macrophages differentially modulate cell death after brain injury caused by oxygen-glucose deprivation in organotypic brain slices
    Sylvie Girard
    Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Glia 61:813-24. 2013
    ..These data suggest that macrophages infiltrating the brain from the periphery after an injury may be cytotoxic, independently of their phenotype, while microglia may be protective...
  17. ncbi request reprint Role of P2X7 receptors in ischemic and excitotoxic brain injury in vivo
    Rosalind A Le Feuvre
    School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK
    J Cereb Blood Flow Metab 23:381-4. 2003
    ..Treatment of mice with P2X7 antagonists did not affect ischemic or excitotoxic cell death, suggesting that P2X7 receptors are not primary mediators of experimentally induced neuronal death...
  18. pmc Two zinc uptake systems contribute to the full virulence of Listeria monocytogenes during growth in vitro and in vivo
    David Corbett
    Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Infect Immun 80:14-21. 2012
    ..monocytogenes can respond to zinc deficiency within a variety of environments and during different stages of infection, with each system making distinct contributions under different stress conditions...
  19. pmc Interleukin-1α expression precedes IL-1β after ischemic brain injury and is localised to areas of focal neuronal loss and penumbral tissues
    Nadia M Luheshi
    University of Manchester, UK
    J Neuroinflammation 8:186. 2011
    ..In order to determine this we investigated the temporal and spatial profiles of IL-1α and IL-1β expression after cerebral ischemia...
  20. pmc Interleukin-1 receptor antagonist is beneficial after subarachnoid haemorrhage in rat by blocking haem-driven inflammatory pathology
    Andrew D Greenhalgh
    Faculty of Life Sciences, University of Manchester, Manchester, M13 9PL, UK
    Dis Model Mech 5:823-33. 2012
    ..These data provide new insights into inflammation after SAH-induced brain injury and suggest IL-1Ra as a candidate therapeutic for the disease...
  21. pmc Agonist-induced regulation of mitochondrial and endoplasmic reticulum motility
    David Brough
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
    Biochem J 392:291-7. 2005
    ....
  22. pmc Efficient discovery of anti-inflammatory small-molecule combinations using evolutionary computing
    Ben G Small
    Doctoral Training Centre, Integrative Systems Biology Molecules to Life, Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK
    Nat Chem Biol 7:902-8. 2011
    ..Evolutionary searches provide a powerful and general approach to the discovery of new combinations of pharmacological agents with therapeutic indices potentially greater than those of single drugs...
  23. pmc Nuclear retention of IL-1 alpha by necrotic cells: a mechanism to dampen sterile inflammation
    Nadia M Luheshi
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, UK
    Eur J Immunol 39:2973-80. 2009
    ..The remaining IL-1 alpha was immobilized in the nuclei of the dead cells. Thus, nuclear retention of IL-1 alpha may serve to limit inflammation following cell death...
  24. ncbi request reprint Extracellular ATP and P2X7 receptors in neurodegeneration
    Rosalind Le Feuvre
    School of Biological Sciences, University of Manchester, Stopford Building 1 204, Oxford Road, Manchester, M13 9PT, UK
    Eur J Pharmacol 447:261-9. 2002
    ..This review discusses the potential involvement of extracellular ATP and P2X7 receptors as regulators of interleukin-1-mediated neuropathologies and thus as a mediator of cell death following pathological insults...
  25. ncbi request reprint A selective, non-peptide caspase-1 inhibitor, VRT-018858, markedly reduces brain damage induced by transient ischemia in the rat
    Jerard Ross
    Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester, M13 9PT, UK
    Neuropharmacology 53:638-42. 2007
    ..These data confirm caspase-1 as an important target for intervention in acute CNS injury, and propose a new class of caspase-1 inhibitors as highly effective neuroprotective agents...
  26. ncbi request reprint Mobility of proteins associated with the plasma membrane by interaction with inositol lipids
    David Brough
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
    J Cell Sci 118:3019-25. 2005
    ..Moreover, the mobility of GAP1(IP4BP) is not detectably altered by the generation of either of its two potential regulators, Ins(1,3,4,5)P(4) or PtdIns(3,4,5)P(3)...
  27. pmc Obese mice exhibit an altered behavioural and inflammatory response to lipopolysaccharide
    Catherine B Lawrence
    AV Hill Building, Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK
    Dis Model Mech 5:649-59. 2012
    ..In summary, DIO and ob/ob mice displayed an altered behavioural response and cytokine release to systemic inflammatory challenge. These findings could help explain why obese humans show increased sensitivity to infections...
  28. pmc NLRP3-Inflammasome Activating DAMPs Stimulate an Inflammatory Response in Glia in the Absence of Priming Which Contributes to Brain Inflammation after Injury
    Catherine Diane Savage
    Faculty of Life Sciences, University of Manchester Manchester, UK
    Front Immunol 3:288. 2012
    ..This is markedly enhanced by DAMP-induced IL-1-release-dependent responses that require a sterile endogenous priming stimulus such as SAA...
  29. doi request reprint Regulation of interleukin-1 in acute brain injury
    David Brough
    Faculty of Life Sciences, University of Manchester, AV Hill Building, Oxford Road, Manchester M13 9PT, UK
    Trends Pharmacol Sci 32:617-22. 2011
    ..Advances are summarised here in the context of addressing how dampening the inflammatory response and actions of IL-1 provides a strategy for reducing damage after acute brain injury such as stroke...
  30. ncbi request reprint Mechanisms of regulation for interleukin-1beta in neurodegenerative disease
    Anastasia Simi
    Faculty of Life Sciences, C 2210 Michael Smith Building, University of Manchester, Manchester M13 9PT, UK
    Neuropharmacology 52:1563-9. 2007
    ..We provide some fresh perspectives on how interleukin-1beta may be regulated and how these mechanisms could be targeted in disease...