Steven J Brookes

Summary

Affiliation: University of Leeds
Country: UK

Publications

  1. ncbi request reprint Intracellular nanosphere subunit assembly as revealed by amelogenin molecular cross-linking studies
    Steven J Brookes
    Department of Oral Biology, Leeds Dental Institute, Leeds, UK, and Oral Research Laboratory, Faculty of Dentistry, University of Oslo, Norway
    Eur J Oral Sci 114:280-4; discussion 285-6, 382. 2006
  2. pmc Is the 32-kDa fragment the functional enamelin unit in all species?
    Steven J Brookes
    Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, UK
    Eur J Oral Sci 119:345-50. 2011
  3. ncbi request reprint Copper ions inhibit the demineralisation of human enamel
    S J Brookes
    Division of Oral Biology, Leeds Dental Institute, LS2 9LU, Leeds, UK
    Arch Oral Biol 48:25-30. 2003
  4. ncbi request reprint Enamelin compartmentalization in developing porcine enamel
    S J Brookes
    Division of Oral Biology, Leeds Dental Institute, Leeds LS2 9LU, UK
    Connect Tissue Res 43:477-81. 2002
  5. ncbi request reprint Spatially related amelogenin interactions in developing rat enamel as revealed by molecular cross-linking studies
    S J Brookes
    Division of Oral Biology, Leeds Dental Institute, LS2 9LU, Leeds, UK
    Arch Oral Biol 45:937-43. 2000
  6. ncbi request reprint Amelin extracellular processing and aggregation during rat incisor amelogenesis
    S J Brookes
    Division of Oral Biology, Leeds Dental Institute, Leeds LS2 9LU, UK
    Arch Oral Biol 46:201-8. 2001
  7. pmc Cellular uptake and processing of enamel matrix derivative by human periodontal ligament fibroblasts
    James D Lees
    Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, England, United Kingdom
    Arch Oral Biol 58:348-54. 2013
  8. pmc Enamel matrix derivative enhances tissue formation around scaffolds used for tissue engineering of ligaments
    Michael P Messenger
    Department of Oral Biology, Leeds Dental Institute, Leeds LS2 9LU, UK
    J Tissue Eng Regen Med 4:96-104. 2010
  9. pmc Identification of mutations in SLC24A4, encoding a potassium-dependent sodium/calcium exchanger, as a cause of amelogenesis imperfecta
    David A Parry
    Leeds Institute of Molecular Medicine, St James s University Hospital, University of Leeds, Leeds, UK
    Am J Hum Genet 92:307-12. 2013
  10. doi request reprint Enamel matrix derivative stimulates expression and secretion of resistin in mesenchymal cells
    Maria V Obregon-Whittle
    Department of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, Norway
    Eur J Oral Sci 119:366-72. 2011

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Intracellular nanosphere subunit assembly as revealed by amelogenin molecular cross-linking studies
    Steven J Brookes
    Department of Oral Biology, Leeds Dental Institute, Leeds, UK, and Oral Research Laboratory, Faculty of Dentistry, University of Oslo, Norway
    Eur J Oral Sci 114:280-4; discussion 285-6, 382. 2006
    ..Once secreted, these prefabricated subunits assemble further to form the mature full-size nanospheres containing hundreds of individual amelogenins characteristic of enamel matrix...
  2. pmc Is the 32-kDa fragment the functional enamelin unit in all species?
    Steven J Brookes
    Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, UK
    Eur J Oral Sci 119:345-50. 2011
    ..The pig may be an atypical model in terms of enamelin biochemistry and function, and caution should be exercised when assigning functional roles to the 32-kDa enamelin as a discrete enamel matrix entity...
  3. ncbi request reprint Copper ions inhibit the demineralisation of human enamel
    S J Brookes
    Division of Oral Biology, Leeds Dental Institute, LS2 9LU, Leeds, UK
    Arch Oral Biol 48:25-30. 2003
    ..The well-known cariostatic properties of Cu2+ may therefore be due not only to its ability to inhibit bacterial growth but also to its ability to directly inhibit acid dissolution of enamel...
  4. ncbi request reprint Enamelin compartmentalization in developing porcine enamel
    S J Brookes
    Division of Oral Biology, Leeds Dental Institute, Leeds LS2 9LU, UK
    Connect Tissue Res 43:477-81. 2002
    ..These products, appearing in the deeper enamel layers, may serve to influence crystal growth kinetics in the absence of any mineral-binding amelogenins...
  5. ncbi request reprint Spatially related amelogenin interactions in developing rat enamel as revealed by molecular cross-linking studies
    S J Brookes
    Division of Oral Biology, Leeds Dental Institute, LS2 9LU, Leeds, UK
    Arch Oral Biol 45:937-43. 2000
    ....
  6. ncbi request reprint Amelin extracellular processing and aggregation during rat incisor amelogenesis
    S J Brookes
    Division of Oral Biology, Leeds Dental Institute, Leeds LS2 9LU, UK
    Arch Oral Biol 46:201-8. 2001
    ..The results provide additional information on amelin processing and degradation, and on how such processing influences the solubility and aggregative properties of amelin-derived proteins...
  7. pmc Cellular uptake and processing of enamel matrix derivative by human periodontal ligament fibroblasts
    James D Lees
    Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, England, United Kingdom
    Arch Oral Biol 58:348-54. 2013
    ..Our objective was to investigate interactions between EMD and human periodontal ligament (HPDL) fibroblasts in vitro...
  8. pmc Enamel matrix derivative enhances tissue formation around scaffolds used for tissue engineering of ligaments
    Michael P Messenger
    Department of Oral Biology, Leeds Dental Institute, Leeds LS2 9LU, UK
    J Tissue Eng Regen Med 4:96-104. 2010
    ..Additionally, it was shown that EMD enhanced TGFbeta1 synthesis of synovial cells, suggesting a potential mode of action for EMD's capacity to stimulate tissue regeneration...
  9. pmc Identification of mutations in SLC24A4, encoding a potassium-dependent sodium/calcium exchanger, as a cause of amelogenesis imperfecta
    David A Parry
    Leeds Institute of Molecular Medicine, St James s University Hospital, University of Leeds, Leeds, UK
    Am J Hum Genet 92:307-12. 2013
    ..These findings support a key role for SLC24A4 in calcium transport during enamel formation...
  10. doi request reprint Enamel matrix derivative stimulates expression and secretion of resistin in mesenchymal cells
    Maria V Obregon-Whittle
    Department of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, Norway
    Eur J Oral Sci 119:366-72. 2011
    ..Our results indicate that resistin may play a role in mediating the biological effect of EMD in mesenchymal tissues...
  11. ncbi request reprint Self-assembling peptide scaffolds promote enamel remineralization
    J Kirkham
    Department of Oral Biology, Leeds Dental Institute, University of Leeds, Clarendon Way, Leeds LS2 9LU, UK
    J Dent Res 86:426-30. 2007
    ..The assembled peptide was also capable of inducing hydroxyapatite nucleation de novo. The results suggest that self-assembling peptides may be useful in the modulation of mineral behavior during in situ dental tissue engineering...
  12. ncbi request reprint Plaque biofilms: the effect of chemical environment on natural human plaque biofilm architecture
    C Robinson
    Division of Oral Biology, the Dental Institute Leeds, UK
    Arch Oral Biol 51:1006-14. 2006
    ..Plaque biofilms were robust and little affected by mechanical manipulation, high ionic strength or low pH (2.5). Detergent (SLS), however, often appeared to either remove biomass and/or dramatically reduce its density...
  13. doi request reprint The structure and composition of deciduous enamel affected by local hypoplastic autosomal dominant amelogenesis imperfecta resulting from an ENAM mutation
    R C Shore
    Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, UK
    Cells Tissues Organs 191:301-6. 2010
    ....
  14. ncbi request reprint Surface chemistry of enamel apatite during maturation in relation to pH: implications for protein removal and crystal growth
    C Robinson
    Division of Oral Biology, Leeds Dental Institute, University of Leeds, UK
    Arch Oral Biol 50:267-70. 2005
    ..Instability of the crystal surface could also play a role...
  15. ncbi request reprint The effect of fluoride on the developing tooth
    C Robinson
    Leeds Dental Institute, University of Leeds, Leeds, UK
    Caries Res 38:268-76. 2004
    ....
  16. ncbi request reprint Subunit structures in hydroxyapatite crystal development in enamel: implications for amelogenesis imperfecta
    C Robinson
    Division of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, United Kingdom
    Connect Tissue Res 44:65-71. 2003
    ..Mutation of matrix or processing enzymes leading to defective processing may have impaired mineral initiation, fusion, and subsequent growth...
  17. ncbi request reprint The effect of copper on demineralization of dental enamel
    A Z Abdullah
    Paediatric Dentistry, Leeds Dental Institute, University of Leeds, Leeds, UK
    J Dent Res 85:1011-5. 2006
    ..Significantly less demineralization was observed with Cu2+ and fluoride in combination than with fluoride treatment alone (p < 0.05), whereas copper alone had no significant protective effect...
  18. ncbi request reprint Characterization of a porcine amelogenin preparation, EMDOGAIN, a biological treatment for periodontal disease
    J Maycock
    Division of Oral Biology, Leeds Dental Institute, Clarendon Way, Leeds, LS2 9LU, United Kingdom
    Connect Tissue Res 43:472-6. 2002
    ..Both metalloendoproteases and serine protease activity were revealed in both EMDOGAIN and developing enamel. The roles of the amelogenin and enzyme components, if any, in periodontal regeneration are unknown...
  19. ncbi request reprint The chemical composition of tooth enamel in junctional epidermolysis bullosa
    J Kirkham
    Division of Oral Biology, Leeds Dental Institute, Clarendon Way, Leeds, UK
    Arch Oral Biol 45:377-86. 2000
    ..It is concluded that EBJ enamel is developmentally compromised and that the enamel defects are commensurate with the reported genetic lesions...
  20. ncbi request reprint A method for the quantitative site-specific study of the biochemistry within dental plaque biofilms formed in vivo
    C Robinson
    Division of Oral Biology, Leeds Dental Institute, UK
    Caries Res 31:194-200. 1997
    ....
  21. ncbi request reprint Identification of human serum albumin in human caries lesions of enamel: the role of putative inhibitors of remineralisation
    C Robinson
    Division of Oral Biology, Leeds Dental Institute, UK
    Caries Res 32:193-9. 1998
    ..The ability of albumin to bind and to inhibit growth of calcium phosphate crystals raises the question as to the possible role of such a molecule in the development of carious lesions...
  22. ncbi request reprint Atomic force microscopy studies of crystal surface topology during enamel development
    J Kirkham
    Division of Oral Biology, Leeds Dental Institute, UK
    Connect Tissue Res 38:91-100; discussion 139-45. 1998
    ..The AFM may prove an important adjunct in determination of the mechanisms controlling crystal size and morphology in skeletal tissues...
  23. ncbi request reprint Effect of experimental fluorosis on the surface topography of developing enamel crystals
    J Kirkham
    Division of Oral Biology, Leeds Dental Institute, University of Leeds, UK
    Caries Res 35:50-6. 2001
    ..These would be expected to affect crystal growth during maturation, resulting in the characteristic porous appearance of fluorotic lesions in mature teeth...
  24. ncbi request reprint The chemistry of enamel caries
    C Robinson
    University of Leeds, Leeds Dental Institute, Division of Oral Biology, United Kingdom
    Crit Rev Oral Biol Med 11:481-95. 2000
    ..Elucidation of the role of organic material may also reveal putative mechanisms for encouraging repair and/or protecting the enamel mineral...
  25. ncbi request reprint Evidence for charge domains on developing enamel crystal surfaces
    J Kirkham
    Division of Oral Biology, Leeds Dental Institute, The University of Leeds, UK
    J Dent Res 79:1943-7. 2000
    ..Alternatively, the alternating array of charge on the crystal surfaces may reflect the original relationship with, and influence of, matrix interaction with the crystal surfaces during crystal growth...
  26. ncbi request reprint The developing enamel matrix: nature and function
    C Robinson
    Division of Oral Biology, Leeds Dental Institute, University of Leeds, UK
    Eur J Oral Sci 106:282-91. 1998
    ..Failure of these mechanisms could lead to incomplete maturation of the enamel and the eruption of dysplastic tissue...
  27. ncbi request reprint In vitro studies of the penetration of adhesive resins into artificial caries-like lesions
    C Robinson
    Division of Oral Biology, Leeds Dental Institute, Leeds, UK
    Caries Res 35:136-41. 2001
    ..The development of such treatment strategies could offer potential noninvasive means of treating early enamel lesions...
  28. ncbi request reprint Inheritance pattern and elemental composition of enamel affected by hypomaturation amelogenesis imperfecta
    R C Shore
    Division of Oral Biology, Leeds Dental Institute, Clarendon Way, Leeds LS2 9LU, UK
    Connect Tissue Res 43:466-71. 2002
    ..The large increase in carbon content, not matched by an equivalent increase in nitrogen or oxygen, suggests a possible increased lipid content. In those teeth with elevated nitrogen levels there may also be retained protein...
  29. ncbi request reprint Distribution of exogenous proteins in caries lesions in relation to the pattern of demineralisation
    R C Shore
    Division of Oral Biology, Leeds Dental Institute, Leeds, UK
    Caries Res 34:188-93. 2000
    ..The presence of these molecules in a region of the lesion where some potential for reprecipitation may exist will have important implications with respect to lesion progression...
  30. pmc Mutations in C4orf26, encoding a peptide with in vitro hydroxyapatite crystal nucleation and growth activity, cause amelogenesis imperfecta
    David A Parry
    Leeds Institute of Molecular Medicine, St James s University Hospital, University of Leeds, LS9 7TF Leeds, UK
    Am J Hum Genet 91:565-71. 2012
    ..A mineral nucleation assay showed that the protein's phosphorylated C terminus has the capacity to promote nucleation of hydroxyapatite, suggesting a possible function in enamel mineralization during amelogenesis...
  31. ncbi request reprint Evidence for direct amelogenin-target cell interactions using dynamic force spectroscopy
    Jennifer Kirkham
    Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, UK
    Eur J Oral Sci 114:219-24; discussion 254-6, 381-2. 2006
    ..These data suggest that amelogenin interacts with potential target cells with forces characteristic of specific ligand-receptor binding, suggesting a direct effect for amelogenin at target cell membranes...
  32. doi request reprint Ameloblastin expression and putative autoregulation in mesenchymal cells suggest a role in early bone formation and repair
    Margareth V Tamburstuen
    Department of Biomaterials, Faculty of Dentistry, University of Oslo UiO, Oslo, Norway
    Bone 48:406-13. 2011
    ..Expression of ameloblastin was also detected in newly formed bone in experimental bone defects in adult rats. Together these findings suggest a role for this protein in early bone formation and repair...
  33. pmc Enamel matrix proteins; old molecules for new applications
    S P Lyngstadaas
    Department of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, Norway
    Orthod Craniofac Res 12:243-53. 2009
    ..New experiments now confirm that amelogenins have potential for being used also in the fields of endodontics, bone regeneration, implantology, traumatology, and wound care...
  34. ncbi request reprint The potential use of enamel matrix derivative for in situ anterior cruciate ligament tissue engineering: a translational in vitro investigation
    Michael P Messenger
    Academic Unit of Musculo Skeletal Disease, Leeds Dental Institute, Faculty of Medicine and Health, The University of Leeds, Leeds, United Kingdom
    Tissue Eng 13:2041-51. 2007
    ..However, its inhibitory effect on synovial cell attachment in vitro implies that it may only be suited to post-operative administration...
  35. ncbi request reprint Isolation and characterisation of an alternatively-spliced rat amelogenin cDNA: LRAP--a highly conserved, functional alternatively-spliced amelogenin?
    W A Bonass
    Leeds Dental Institute, University of Leeds, UK
    Biochim Biophys Acta 1219:690-2. 1994
    ..The conservation of this truncated polypeptide across the species suggests that it may have an important role in the formation of tooth enamel...
  36. pmc The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice
    Martin J Barron
    Faculty of Life Sciences, University of Manchester, Manchester, UK
    Hum Mol Genet 17:3509-20. 2008
    ..Importantly, our results show that integrity of the SI-ameloblast interface is essential for normal enamel mineralization...