M D Brand

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. pmc Experimental assessment of bioenergetic differences caused by the common European mitochondrial DNA haplogroups H and T
    Taku Amo
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
    Gene 411:69-76. 2008
  2. pmc Oxidative damage and phospholipid fatty acyl composition in skeletal muscle mitochondria from mice underexpressing or overexpressing uncoupling protein 3
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U K
    Biochem J 368:597-603. 2002
  3. ncbi The efficiency and plasticity of mitochondrial energy transduction
    M D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 33:897-904. 2005
  4. pmc Proton conductance and fatty acyl composition of liver mitochondria correlates with body mass in birds
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 376:741-8. 2003
  5. ncbi Mitochondrial superoxide: production, biological effects, and activation of uncoupling proteins
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Cambridge CB2 2XY, UK
    Free Radic Biol Med 37:755-67. 2004
  6. pmc The basal proton conductance of mitochondria depends on adenine nucleotide translocase content
    Martin D Brand
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 392:353-62. 2005
  7. ncbi Physiological functions of the mitochondrial uncoupling proteins UCP2 and UCP3
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge, United Kingdom
    Cell Metab 2:85-93. 2005
  8. ncbi Simplifying metabolic complexity
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U K
    Biochem Soc Trans 30:25-30. 2002
  9. ncbi Uncoupling to survive? The role of mitochondrial inefficiency in ageing
    M D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, CB2 2XY, Cambridge, UK
    Exp Gerontol 35:811-20. 2000
  10. ncbi Superoxide activates mitochondrial uncoupling proteins
    Karim S Echtay
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Nature 415:96-9. 2002

Detail Information

Publications89

  1. pmc Experimental assessment of bioenergetic differences caused by the common European mitochondrial DNA haplogroups H and T
    Taku Amo
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
    Gene 411:69-76. 2008
    ..We found that there were no functionally-important bioenergetic differences between mitochondria bearing these haplogroups, using either isolated mitochondria or mitochondria within cells...
  2. pmc Oxidative damage and phospholipid fatty acyl composition in skeletal muscle mitochondria from mice underexpressing or overexpressing uncoupling protein 3
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U K
    Biochem J 368:597-603. 2002
    ....
  3. ncbi The efficiency and plasticity of mitochondrial energy transduction
    M D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 33:897-904. 2005
    ..UCPs may also integrate inputs from different fuels in pancreatic beta-cells and modulate insulin secretion. They are exciting potential targets for treatment of obesity, cachexia, aging and diabetes...
  4. pmc Proton conductance and fatty acyl composition of liver mitochondria correlates with body mass in birds
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 376:741-8. 2003
    ..These correlations echo those seen in mammalian liver mitochondria, suggesting that they too are general for endotherms...
  5. ncbi Mitochondrial superoxide: production, biological effects, and activation of uncoupling proteins
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Cambridge CB2 2XY, UK
    Free Radic Biol Med 37:755-67. 2004
    ..More recently evolved roles of uncoupling proteins are in adaptive thermogenesis (UCP1) and perhaps as part of a signaling pathway to regulate insulin secretion in pancreatic beta cells (UCP2)...
  6. pmc The basal proton conductance of mitochondria depends on adenine nucleotide translocase content
    Martin D Brand
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 392:353-62. 2005
    ..We conclude that half to two-thirds of the basal proton conductance of mitochondria is catalysed by the adenine nucleotide carrier, independently of its ATP/ADP exchange or fatty-acid-dependent proton-leak functions...
  7. ncbi Physiological functions of the mitochondrial uncoupling proteins UCP2 and UCP3
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge, United Kingdom
    Cell Metab 2:85-93. 2005
    ..Evidence that they export fatty acids physiologically is weak. UCP2 and UCP3 are important potential targets for treatment of aging, degenerative diseases, diabetes, and perhaps obesity...
  8. ncbi Simplifying metabolic complexity
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U K
    Biochem Soc Trans 30:25-30. 2002
    ..Recently, we have applied modular control analysis to modern DNA microarray expression profiling to measure the importance of different groups of mRNA transcripts in mediating physiological responses...
  9. ncbi Uncoupling to survive? The role of mitochondrial inefficiency in ageing
    M D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, CB2 2XY, Cambridge, UK
    Exp Gerontol 35:811-20. 2000
    ..However, to date there is no direct evidence in cells or organisms that mitochondrial proton cycling lowers ROS production or oxidative damage or that it increases lifespan...
  10. ncbi Superoxide activates mitochondrial uncoupling proteins
    Karim S Echtay
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Nature 415:96-9. 2002
    ..Our findings indicate that the interaction of superoxide with UCPs may be a mechanism for decreasing the concentrations of reactive oxygen species inside mitochondria...
  11. ncbi Superoxide activates a GDP-sensitive proton conductance in skeletal muscle mitochondria from king penguin (Aptenodytes patagonicus)
    Darren A Talbot
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, CB2 2XY, Cambridge, UK
    Biochem Biophys Res Commun 312:983-8. 2003
    ..In the absence of superoxide there is no GDP-sensitive component of the proton conductance from penguin muscle mitochondria demonstrating that avUCP plays no role in the basal proton leak...
  12. ncbi Superoxide activates mitochondrial uncoupling protein 2 from the matrix side. Studies using targeted antioxidants
    Karim S Echtay
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    J Biol Chem 277:47129-35. 2002
    ..We conclude that superoxide (or its products) exerts its uncoupling effect by activating the proton transport mechanism of uncoupling proteins at the matrix side of the mitochondrial inner membrane...
  13. pmc A signalling role for 4-hydroxy-2-nonenal in regulation of mitochondrial uncoupling
    Karim S Echtay
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    EMBO J 22:4103-10. 2003
    ..Our findings indicate that hydroxynonenal is not merely toxic, but may be a biological signal to induce uncoupling through UCPs and ANT and thus decrease mitochondrial ROS production...
  14. ncbi Topology of superoxide production from different sites in the mitochondrial electron transport chain
    Julie St-Pierre
    MRC Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, England
    J Biol Chem 277:44784-90. 2002
    ..This difference was solely due to an elevated concentration of complex I in rat compared with pigeon heart mitochondria...
  15. ncbi The basal proton conductance of skeletal muscle mitochondria from transgenic mice overexpressing or lacking uncoupling protein-3
    Susana Cadenas
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 277:2773-8. 2002
    ..We conclude that uncoupling in UCP3-tg mice is an artifact of transgenic expression, and that UCP3 does not catalyze the basal proton conductance of skeletal muscle mitochondria in the absence of activators such as superoxide...
  16. pmc Synergy of fatty acid and reactive alkenal activation of proton conductance through uncoupling protein 1 in mitochondria
    Telma C Esteves
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 395:619-28. 2006
    ..These results show that reactive alkenals activate the proton conductance of UCP1 more strongly when fatty acids are also added, with implications for both mechanistic and physiological models of UCP1 activation...
  17. pmc Artifactual uncoupling by uncoupling protein 3 in yeast mitochondria at the concentrations found in mouse and rat skeletal-muscle mitochondria
    James A Harper
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U K
    Biochem J 361:49-56. 2002
    ..We conclude that the uncoupling of yeast mitochondria by high levels of UCP3 expression is entirely an artifact and provides no evidence for any native uncoupling activity of the protein...
  18. ncbi Coenzyme Q induces GDP-sensitive proton conductance in kidney mitochondria
    K S Echtay
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem Soc Trans 29:763-8. 2001
    ..We conclude that CoQ acted in mitochondria through production of superoxide, which mediated uncoupling, probably by acting through uncoupling protein 2...
  19. ncbi Production of endogenous matrix superoxide from mitochondrial complex I leads to activation of uncoupling protein 3
    Darren A Talbot
    MRC Dunn Human Nutrition Unit, Hills Road, CB2 2XY, Cambridge, UK
    FEBS Lett 556:111-5. 2004
    ..Thus, endogenous superoxide can activate the proton conductance of UCP3, which in turn limits mitochondrial superoxide production. These observations provide a departure point for studies under more physiological conditions...
  20. pmc A mitochondrial uncoupling artifact can be caused by expression of uncoupling protein 1 in yeast
    J A Stuart
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 356:779-89. 2001
    ..This observation might be important in the interpretation of results from experiments in which the functions of UCP1 homologues are verified by their ability to uncouple yeast mitochondria...
  21. ncbi Physiological levels of mammalian uncoupling protein 2 do not uncouple yeast mitochondria
    J A Stuart
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 276:18633-9. 2001
    ..These observations suggest that uncoupling of yeast mitochondria by UCP2 is an overexpression artifact leading to compromised mitochondrial integrity...
  22. pmc Uncoupling protein and ATP/ADP carrier increase mitochondrial proton conductance after cold adaptation of king penguins
    Darren A Talbot
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    J Physiol 558:123-35. 2004
    ....
  23. ncbi Mitochondrial uncoupling as a target for drug development for the treatment of obesity
    J A Harper
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Obes Rev 2:255-65. 2001
    ..Specific uncoupling of muscle or brown adipocyte mitochondria remains an attractive target for the development of antiobesity drugs...
  24. ncbi Uncoupling protein 3 protects aconitase against inactivation in isolated skeletal muscle mitochondria
    Darren A Talbot
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochim Biophys Acta 1709:150-6. 2005
    ..The IC(50) for GDP is about 100 microM. These findings support the proposal that UCP3 attenuates endogenous radical production by the mitochondrial electron transport chain at high protonmotive force...
  25. ncbi Cold-induced alterations of phospholipid fatty acyl composition in brown adipose tissue mitochondria are independent of uncoupling protein-1
    Augustine Ocloo
    Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge, U K
    Am J Physiol Regul Integr Comp Physiol 293:R1086-93. 2007
    ..Thus, the alterations in brown adipose tissue phospholipid acyl composition are revealed to be an independent part of the recruitment process, and their functional significance for thermogenesis should be elucidated...
  26. ncbi Primary causes of decreased mitochondrial oxygen consumption during metabolic depression in snail cells
    Tammie Bishop
    Medical Research Council, Dunn Human Nutrition Unit, Cambridge CB2 2XY, United Kingdom
    Am J Physiol Regul Integr Comp Physiol 282:R372-82. 2002
    ..At least 75% of the total response of mitochondrial respiration to estivation was caused by primary changes in the kinetics of substrate oxidation, with only 25% or less of the response occurring through primary effects on ATP turnover...
  27. ncbi Mitochondrial proton leak and the uncoupling protein 1 homologues
    J A Stuart
    MRC Dunn Human Nutrition Unit, Cambridge, UK
    Biochim Biophys Acta 1504:144-58. 2001
    ..The physiological role of the proton leak, and of the uncoupling protein 1 homologues, remains similarly unclear...
  28. ncbi Processes contributing to metabolic depression in hepatopancreas cells from the snail Helix aspersa
    T Bishop
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    J Exp Biol 203:3603-12. 2000
    ..Thus, the fraction of cellular respiration devoted to different processes remained constant and the cellular energy balance was preserved in the hypometabolic state...
  29. pmc High membrane potential promotes alkenal-induced mitochondrial uncoupling and influences adenine nucleotide translocase conformation
    Vian Azzu
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge, CB2 0XY, UK
    Biochem J 413:323-32. 2008
    ..We propose a model to relate adenine nucleotide translocase conformation to proton conductance in the presence or absence of 4-hydroxynonenal and/or carboxyatractylate...
  30. ncbi Research on mitochondria and aging, 2006-2007
    Adrian J Lambert
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge, UK
    Aging Cell 6:417-20. 2007
    ..We also list other relevant articles of interest and suggest some key challenges for the field in the near future...
  31. doi Measuring mitochondrial bioenergetics in INS-1E insulinoma cells
    Charles Affourtit
    MRC Dunn Human Nutrition Unit, Cambridge, United Kingdom
    Methods Enzymol 457:405-24. 2009
    ..We also introduce a method to determine coupling efficiencies noninvasively in attached cells and discuss theoretical and practical aspects of a modular-kinetic approach to describe and understand cellular bioenergetics...
  32. ncbi Does any yeast mitochondrial carrier have a native uncoupling protein function?
    Damien Roussel
    MRC Dunn Human Nutrition Unit, Cambridge, United Kingdom
    J Bioenerg Biomembr 34:165-76. 2002
    ..If a specific protein is responsible for the basal proton conductance of yeast mitochondria, its identity remains unknown...
  33. pmc Superoxide production by NADH:ubiquinone oxidoreductase (complex I) depends on the pH gradient across the mitochondrial inner membrane
    Adrian J Lambert
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 382:511-7. 2004
    ....
  34. ncbi The topology of superoxide production by complex III and glycerol 3-phosphate dehydrogenase in Drosophila mitochondria
    Satomi Miwa
    MRC Dunn Human Nutrition Unit, Cambridge CB2 2XY, UK
    Biochim Biophys Acta 1709:214-9. 2005
    ....
  35. doi Low complex I content explains the low hydrogen peroxide production rate of heart mitochondria from the long-lived pigeon, Columba livia
    Adrian J Lambert
    Medical Research Council Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 0XY, UK
    Aging Cell 9:78-91. 2010
    ..We conclude that mitochondria from the long-lived pigeon display low rates of hydrogen peroxide production because they have low levels of complex I...
  36. pmc Nucleotide binding to human uncoupling protein-2 refolded from bacterial inclusion bodies
    Mika B Jekabsons
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 366:565-71. 2002
    ....
  37. ncbi Transcript and metabolite analysis of the effects of tamoxifen in rat liver reveals inhibition of fatty acid synthesis in the presence of hepatic steatosis
    Christopher J Lelliott
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Cambridge, UK
    FASEB J 19:1108-19. 2005
    ..Given the broader importance of hepatic steatosis, the novel molecular mechanism revealed in this study should be examined in other forms of steatosis and nonalcoholic steatohepatitis...
  38. ncbi Inhibitors of the quinone-binding site allow rapid superoxide production from mitochondrial NADH:ubiquinone oxidoreductase (complex I)
    Adrian J Lambert
    Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:39414-20. 2004
    ....
  39. ncbi The reactions catalysed by the mitochondrial uncoupling proteins UCP2 and UCP3
    Telma C Esteves
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochim Biophys Acta 1709:35-44. 2005
    ..However, they do not catalyse proton leak in the absence of such acute activation. They can also catalyse export of fatty acid and other anions, although the relationship of anion transport to proton transport remains controversial...
  40. pmc Expression of human uncoupling protein-3 in Drosophila insulin-producing cells increases insulin-like peptide (DILP) levels and shortens lifespan
    Dickon M Humphrey
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Exp Gerontol 44:316-27. 2009
    ..These data suggest that lowering mitochondrial coupling by high expression of hUCP3 alters mNSC function in a way that appears to increase DILP-levels in fly heads and lead to a concomitant decrease in lifespan...
  41. doi Uncoupling protein-1 (UCP1) contributes to the basal proton conductance of brown adipose tissue mitochondria
    Nadeene Parker
    MRC Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge, CB2 0XY, UK
    J Bioenerg Biomembr 41:335-42. 2009
    ....
  42. ncbi Prevention of mitochondrial oxidative damage as a therapeutic strategy in diabetes
    Katherine Green
    Medical Research Council Dunn Human Nutrition Unit, Cambridge, UK
    Diabetes 53:S110-8. 2004
    ..Here, we outline the background to these strategies and discuss how antioxidants targeted to mitochondria, or selective mitochondrial uncoupling, may be potential therapies for diabetes...
  43. ncbi Structure and function of mitochondria in hepatopancreas cells from metabolically depressed snails
    T Bishop
    Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, United Kingdom
    Physiol Biochem Zool 75:134-44. 2002
    ..Thus the lower mitochondrial respiration rate in hepatopancreas cells from aestivating snails was not caused by differences in mitochondrial volume or surface density but was associated with intrinsic changes in the mitochondria...
  44. ncbi Top down metabolic control analysis
    M D Brand
    Department of Biochemistry, University of Cambridge, U K
    J Theor Biol 182:351-60. 1996
    ..It discusses the ways the approach has been used in organelles and cells to identify the sites of action of effectors, to measure the distribution of control by blocks of reactions, and to measure regulation by effectors...
  45. ncbi The effect of metabolic depression on proton leak rate in mitochondria from hibernating frogs
    J St-Pierre
    Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK
    J Exp Biol 203:1469-76. 2000
    ....
  46. ncbi The significance and mechanism of mitochondrial proton conductance
    M D Brand
    Department of Biochemistry, University of Cambridge, UK
    Int J Obes Relat Metab Disord 23:S4-11. 1999
    ..UCP1 catalyses a regulated inducible proton conductance in brown adipose tissue and the possibility remains open that UCP2 and UCP3 have a similar role in other tissues, although this has yet to be demonstrated...
  47. pmc Mitochondria as ATP consumers: cellular treason in anoxia
    J St-Pierre
    Department of Zoology, University of Cambridge, Cambridge, England CB2 3EJ
    Proc Natl Acad Sci U S A 97:8670-4. 2000
    ..However, ATP use by the F(1)F(0)-ATPase is limited in anoxia by a profound inhibition of the enzyme. Even so, ATP use by the F(1)F(0)-ATPase might account for approximately 9% of the ATP turnover in anoxic frog skeletal muscle...
  48. ncbi Extracting meaning from microarray data
    R K Curtis
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U K
    Biochem Soc Trans 31:1474-5. 2003
    ..The application of modular control analysis to microarray data in order to partition and quantify the importance of mRNA clusters in mediating responses is described...
  49. ncbi Regulation analysis of energy metabolism
    M D Brand
    Department of Biochemistry, University of Cambridge, UK
    J Exp Biol 200:193-202. 1997
    ....
  50. ncbi Effects of the mitogen concanavalin A on pathways of thymocyte energy metabolism
    S Krauss
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK
    Biochim Biophys Acta 1412:129-38. 1999
    ....
  51. ncbi Low rates of hydrogen peroxide production by isolated heart mitochondria associate with long maximum lifespan in vertebrate homeotherms
    Adrian J Lambert
    Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Aging Cell 6:607-18. 2007
    ..These findings indicate that enhanced longevity may be causally associated with low free radical production by mitochondria across species over two classes of vertebrate homeotherms...
  52. pmc The regulation and turnover of mitochondrial uncoupling proteins
    Vian Azzu
    Medical Research Council Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, UK
    Biochim Biophys Acta 1797:785-91. 2010
    ..The significant recent progress describing the turnover of novel uncoupling proteins, as well as current views on the physiological roles and regulation of UCPs, is outlined...
  53. ncbi Novel uncoupling proteins
    Charles Affourtit
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Novartis Found Symp 287:70-80; discussion 80-91. 2007
    ..3) UCP3 may catalyse fatty acid transport. (4) UCP2 has a signalling role in pancreatic beta cells, where it attenuates insulin secretion. Other roles remain to be discovered...
  54. pmc The on-off switches of the mitochondrial uncoupling proteins
    Vian Azzu
    MRC Mitochondrial Biology Unit, Cambridge CB2 0XY, UK
    Trends Biochem Sci 35:298-307. 2010
    ..Although much work has been devoted to how these proteins are activated, little is known of the mechanisms that reverse this activation...
  55. pmc Energization-dependent endogenous activation of proton conductance in skeletal muscle mitochondria
    Nadeene Parker
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
    Biochem J 412:131-9. 2008
    ....
  56. doi On the role of uncoupling protein-2 in pancreatic beta cells
    Charles Affourtit
    MRC Dunn Human Nutrition Unit, Cambridge CB2 0XY, UK
    Biochim Biophys Acta 1777:973-9. 2008
    ..With this paper we aim to provide a brief account of the present state of affairs in this field, suggest a physiological role for UCP2, and highlight some of our own recent results...
  57. pmc Rapid turnover of mitochondrial uncoupling protein 3
    Vian Azzu
    Medical Research Council Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, UK
    Biochem J 426:13-7. 2010
    ..This phenotype is in direct contrast with the related proteins UCP1 and the adenine nucleotide translocase, which have long half-lives. Therefore UCP3 is turned over rapidly in multiple cell types in a proteasome-dependent manner...
  58. pmc Stimulation of mitochondrial proton conductance by hydroxynonenal requires a high membrane potential
    Nadeene Parker
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Biosci Rep 28:83-8. 2008
    ..These results suggest that both endogenous HNE production and high membrane potential are required before mild uncoupling will be triggered to attenuate mitochondrial ROS production...
  59. doi Diphenyleneiodonium acutely inhibits reactive oxygen species production by mitochondrial complex I during reverse, but not forward electron transport
    Adrian J Lambert
    Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
    Biochim Biophys Acta 1777:397-403. 2008
    ....
  60. pmc Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells
    Vian Azzu
    MRC Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Cambridge, UK
    Biochim Biophys Acta 1777:1378-83. 2008
    ..0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h...
  61. doi Dissociation of superoxide production by mitochondrial complex I from NAD(P)H redox state
    Adrian J Lambert
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
    FEBS Lett 582:1711-4. 2008
    ..Our data suggest that less than 10% of the superoxide originates from the flavin site during reverse electron transport from succinate...
  62. pmc Not all mitochondrial carrier proteins support permeability transition pore formation: no involvement of uncoupling protein 1
    Paul G Crichton
    MRC Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, UK
    Biosci Rep 30:187-92. 2010
    ....
  63. ncbi Uncoupling protein-2 contributes significantly to high mitochondrial proton leak in INS-1E insulinoma cells and attenuates glucose-stimulated insulin secretion
    Charles Affourtit
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
    Biochem J 409:199-204. 2008
    ..We propose that the high mitochondrial proton leak in beta-cells is a mechanism which amplifies the effect of physiological UCP2 regulators on cytoplasmic ATP/ADP and hence on insulin secretion...
  64. ncbi Lack of correlation between mitochondrial reactive oxygen species production and life span in Drosophila
    Satomi Miwa
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Ann N Y Acad Sci 1019:388-91. 2004
    ..Our results show two examples in which mitochondrial ROS production and life span are not correlated...
  65. pmc Ubiquinone is not required for proton conductance by uncoupling protein 1 in yeast mitochondria
    Telma C Esteves
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 379:309-15. 2004
    ..The activity of UCP1 was similar in both Q-containing and -deficient yeast mitochondria. We conclude that Q is neither an obligatory cofactor nor an activator of proton transport by UCP1 when it is expressed in yeast mitochondria...
  66. ncbi Control analysis of DNA microarray expression data
    R Keira Curtis
    MRC Dunn Human Nutrition Unit, Cambridge, UK
    Mol Biol Rep 29:67-71. 2002
    ..The method is applicable to microarray, transcriptome, proteome and metabolome data...
  67. pmc Stronger control of ATP/ADP by proton leak in pancreatic beta-cells than skeletal muscle mitochondria
    Charles Affourtit
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 393:151-9. 2006
    ....
  68. ncbi Superoxide activates uncoupling proteins by generating carbon-centered radicals and initiating lipid peroxidation: studies using a mitochondria-targeted spin trap derived from alpha-phenyl-N-tert-butylnitrone
    Michael P Murphy
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:48534-45. 2003
    ..Superoxide releases iron from iron-sulfur center proteins, which then generates carbon-centered radicals that initiate lipid peroxidation, yielding breakdown products that activate UCPs...
  69. ncbi Molecular properties of purified human uncoupling protein 2 refolded from bacterial inclusion bodies
    Mika B Jekabsons
    Medical Research Council, Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Bioenerg Biomembr 35:409-18. 2003
    ....
  70. ncbi Analysing microarray data using modular regulation analysis
    R Keira Curtis
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge, CB2 2XY, UK
    Bioinformatics 20:1272-84. 2004
    ..Current analysis methods cannot distinguish between direct and indirect effects on expression, or calculate the relative importance of mRNAs in effecting responses...
  71. ncbi Superoxide and hydrogen peroxide production by Drosophila mitochondria
    Satomi Miwa
    MRC Dunn Human Nutrition Unit, Cambridge, England, United Kingdom
    Free Radic Biol Med 35:938-48. 2003
    ....
  72. ncbi Mitochondrial matrix reactive oxygen species production is very sensitive to mild uncoupling
    S Miwa
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, U K
    Biochem Soc Trans 31:1300-1. 2003
    ..Thus mild uncoupling may be particularly significant for ROS production from complex I on the matrix side of the mitochondrial inner membrane...
  73. pmc Mitochondrial uncouplers with an extraordinary dynamic range
    Phing How Lou
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 407:129-40. 2007
    ....
  74. pmc Degradation of an intramitochondrial protein by the cytosolic proteasome
    Vian Azzu
    MRC Mitochondrial Biology Unit, Hills Road, Cambridge CB2 0XY, UK
    J Cell Sci 123:578-85. 2010
    ..These observations provide the first demonstration that a mitochondrial inner membrane protein is degraded by the cytosolic ubiquitin-proteasome system...
  75. pmc Characterization of the human, mouse and rat PGC1 beta (peroxisome-proliferator-activated receptor-gamma co-activator 1 beta) gene in vitro and in vivo
    Aline Meirhaeghe
    Department of Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge, UK
    Biochem J 373:155-65. 2003
    ..These results suggest that PGC1 beta may play a role in constitutive adrenergic-independent mitochondrial biogenesis...
  76. ncbi Quantitation of signal transduction
    S Krauss
    Department of Biochemistry, University of Cambridge, Cambridge, UK
    FASEB J 14:2581-8. 2000
    ..The approach allows a quantitative description of the relative importance of signal transduction pathways and the routes by which they activate a specific cellular process. It should be widely applicable...
  77. pmc Were inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines
    Taku Amo
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 404:345-51. 2007
    ..Contrary to the predictions of this hypothesis, mitochondria from Arctic haplogroups had similar or even greater coupling efficiency than mitochondria from tropical haplogroups...
  78. doi Reactive oxygen species production by mitochondria
    Adrian J Lambert
    MRC Mitochondrial Biology Unit, Cambridge, UK
    Methods Mol Biol 554:165-81. 2009
    ..A brief overview of the methods for measuring reactive oxygen species production in isolated mitochondria and cells is also presented...
  79. ncbi Nonsteroidal antiinflammatory drugs and a selective cyclooxygenase 2 inhibitor uncouple mitochondria in intact cells
    Manja M Krause
    University Hospital Charite, Humboldt University Berlin, Berlin, Germany
    Arthritis Rheum 48:1438-44. 2003
    ..We investigated the occurrence of NSAID-induced uncoupling of mitochondria in intact cells (rat thymocytes) compared with the effects of a selective cyclooxygenase 2 (COX-2) inhibitor...
  80. ncbi Uncoupled and surviving: individual mice with high metabolism have greater mitochondrial uncoupling and live longer
    John R Speakman
    Aberdeen Centre for Energy Regulation and Obesity ACERO, School of Biological Sciences, University of Aberdeen, Aberdeen, Scotland, UK AB24 2TZ
    Aging Cell 3:87-95. 2004
    ..Individuals with high metabolism were therefore more uncoupled, had greater resting and total daily energy expenditures and survived longest - supporting the 'uncoupling to survive' hypothesis...
  81. ncbi Tissue-specific depression of mitochondrial proton leak and substrate oxidation in hibernating arctic ground squirrels
    Jamie L Barger
    Institute of Arctic Biology, University of Alaska, Fairbanks, Alaska 99775, USA
    Am J Physiol Regul Integr Comp Physiol 284:R1306-13. 2003
    ..These results suggest that mitochondrial proton conductance is unchanged during hibernation and that the reduced metabolism in hibernators is a partial consequence of tissue-specific depression of substrate oxidation...
  82. ncbi Superoxide stimulates a proton leak in potato mitochondria that is related to the activity of uncoupling protein
    Michael J Considine
    Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdom
    J Biol Chem 278:22298-302. 2003
    ..Furthermore, it demonstrates that changes in the amount of StUCP can alter the rate of proton conductance of potato mitochondria...
  83. doi The efficiency of cellular energy transduction and its implications for obesity
    Mary Ellen Harper
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ontario, Canada K1H 8M5
    Annu Rev Nutr 28:13-33. 2008
    ..We discuss the opportunities and pitfalls of targeting these processes as a treatment for obesity by decreasing coupling efficiency and increasing energy expenditure, either directly or through central mechanisms of energy homeostasis...
  84. ncbi The effect of dietary restriction on mitochondrial protein density and flight muscle mitochondrial morphology in Drosophila
    Tapiwanashe Magwere
    University College London, Department of Biology, Darwin Building, Gower Street, London WC1E 6BT, UK
    J Gerontol A Biol Sci Med Sci 61:36-47. 2006
    ..If DR in Drosophila extends life span by reducing ROS production, then it does so through mechanisms that operate only in vivo...
  85. ncbi Hydroxynonenal and uncoupling proteins: a model for protection against oxidative damage
    Karim S Echtay
    Department of Biomedical Sciences, Faculty of Medicine and Medical Sciences, University of Balamand, Tripoli, Lebanon
    Biofactors 24:119-30. 2005
    ..This activation induces mild uncoupling and so diminishes mitochondrial superoxide production, hence protecting against disease and oxidative damage at the expense of energy production...
  86. ncbi Flight activity, mortality rates, and lipoxidative damage in Drosophila
    Tapiwanashe Magwere
    Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    J Gerontol A Biol Sci Med Sci 61:136-45. 2006
    ..The effect of flight activity on insect life span differs considerably from the beneficial effects of exercise in mammals; these differences may be caused by physiological differences between the two taxa...
  87. ncbi Targeting dinitrophenol to mitochondria: limitations to the development of a self-limiting mitochondrial protonophore
    Frances H Blaikie
    Department of Chemistry, University of Otago, P O Box 56, Dunedin, New Zealand
    Biosci Rep 26:231-43. 2006
    ..Therefore MitoDNP did not act as a protonophore, probably because the efflux of deprotonated MitoDNP was inhibited...
  88. ncbi Functional characterisation of UCP1 in the common carp: uncoupling activity in liver mitochondria and cold-induced expression in the brain
    Martin Jastroch
    Animal Physiology, Department of Biology, Philipps University Marburg, Karl von Frisch Str 8, Marburg, 35032, Germany
    J Comp Physiol B 177:743-52. 2007
    ..Taken together, this study characterises uncoupling protein activity in an ectotherm for the first time...
  89. ncbi 4-hydroxy-2-nonenal and uncoupling proteins: an approach for regulation of mitochondrial ROS production
    Karim S Echtay
    Department of Biomedical Sciences, Faculty of Medicine and Medical Sciences, University of Balamand, Tripoli, Lebanon
    Redox Rep 12:26-9. 2007
    ....