Research Topics
| Martin J BoothSummaryAffiliation: University of Oxford Country: UK Publications
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Detail Information
Publications
Adaptive aberration correction in a confocal microscopeMartin J Booth
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, United Kingdom
Proc Natl Acad Sci U S A 99:5788-92. 2002..Aberration corrected images of biological specimens show considerable improvement in contrast and apparent restoration of axial resolution...
Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopyTomoko Watanabe
Department of Physiology Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK
BMC Cell Biol 11:38. 2010..Harmonic generation microscopy (HGM) allows one to image LD without the use of exogenous labels. Adaptive optics can be used to correct aberrations that would otherwise degrade the quality and information content of images...
Strategies for the compensation of specimen-induced spherical aberration in confocal microscopy of skinM J Booth
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK
J Microsc 200:68-74. 2000..The methods include the use of an iris to reduce the pupil area, changing the refractive index of the immersion medium and using a lens with variable coverglass correction...
Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correctionM J Booth
University of Oxford, Department of Engineering Science, Parks Road, Oxford OX1 3PJ, United Kingdom
J Biomed Opt 6:266-72. 2001..An approximation based upon geometrical optics is also introduced which shows that the axial resolution of heavily aberrated PSFs is roughly proportional to focusing depth...
New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopyMartin J Booth
Department of Engineering Science, University of Oxford, UK
J Opt Soc Am A Opt Image Sci Vis 19:2112-20. 2002..Four wave-front sensor configurations are presented and their input response is characterized. Sensitivity matrices and axial responses are presented...
Low-cost, frequency-domain, fluorescence lifetime confocal microscopyM J Booth
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK
J Microsc 214:36-42. 2004..We show fluorescence lifetime images of bead objects and fluorescence lifetime images of biological specimens from a single confocal scan...
Full spectrum filterless fluorescence microscopyM J Booth
Department of Engineering Science, University of Oxford Parks Road, Oxford OX13PJ, United Kingdom
J Microsc 237:103-9. 2010..Full spectrum fluorescence images of biological specimens are demonstrated. As this system is more versatile than the standard fluorescence microscope, it could be of benefit in many fluorescence imaging applications...
Adaptive optics in microscopyMartin J Booth
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK
Philos Trans A Math Phys Eng Sci 365:2829-43. 2007..Applications of adaptive optics in the related areas of optical data storage, optical tweezers and micro/nanofabrication are also reviewed...
Real-time extended depth of field microscopyEdward J Botcherby
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, United Kingdom
Opt Express 16:21843-8. 2008..We also demonstrate a simple modification that enables extended depth of field images to be acquired from different angles of perspective, where the angle can be changed over a continuous range by the user in real-time...
Adaptive optics for structured illumination microscopyDelphine Debarre
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, United Kingdom
Opt Express 16:9290-305. 2008..Aberration corrected imaging is demonstrated using fixed fluorescent specimens. Images are further improved using differential aberration imaging for reduction of background fluorescence...
Aberration-free optical refocusing in high numerical aperture microscopyEdward J Botcherby
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, United Kingdom
Opt Lett 32:2007-9. 2007..4 NA objective lens. As refocusing is implemented remotely from the specimen, this method will enable high axial scan speeds without mechanical interference between the objective lens and the specimen...
Three dimensional laser microfabrication in diamond using a dual adaptive optics systemRichard D Simmonds
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, UK
Opt Express 19:24122-8. 2011....
Image-based adaptive optics for two-photon microscopyDelphine Debarre
Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, United Kingdom
Opt Lett 34:2495-7. 2009..In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues...
Agitation-free multiphoton microscopy of oblique planesChristopher W Smith
Department of Engineering Science, University of Oxford, Oxford, UK
Opt Lett 36:663-5. 2011..To obtain images from oblique planes at high speed, a two-photon system was built wherein a novel optical system is used to provide aberration-free scanning...
Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz ratesEdward J Botcherby
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, United Kingdom
Proc Natl Acad Sci U S A 109:2919-24. 2012..We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level...
Real-time slit scanning microscopy in the meridional planeEdward J Botcherby
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK
Opt Lett 34:1504-6. 2009....
Measurement of specimen-induced aberrations of biological samples using phase stepping interferometryM Schwertner
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK
J Microsc 213:11-9. 2004..Therefore, these higher order modes can be neglected in future adaptive optics sensing and correction schemes implemented into confocal or multiphoton microscopes, leading to more efficient designs...
Adaptive optics for direct laser writing with plasma emission aberration sensingAlexander Jesacher
Department of Engineering Science, University of Oxford, Oxford, United Kingdom
Opt Express 18:656-61. 2010....
Addressable microlens array for parallel laser microfabricationPatrick S Salter
Department of Engineering Science, University of Oxford, Parks Road, Oxford, OX1 3PJ, UK
Opt Lett 36:2302-4. 2011..In addition, the technique allows for homogenizing the intensity of the array of foci and translating spots relative to their natural focus. The technique demonstrates the potential for high efficiency processing of aperiodic structures...
Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetryM Schwertner
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK
J Microsc 215:271-80. 2004..This simple model gave Zernike mode data that are in good agreement with experimental results...
