J J Blow

Summary

Affiliation: University of Dundee
Country: UK

Publications

  1. pmc Mammalian nuclei become licensed for DNA replication during late telophase
    Daniela S Dimitrova
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    J Cell Sci 115:51-9. 2002
  2. pmc The elusive determinants of replication origins
    Silvia Costa
    Division of Gene Regulation and Expression, College of Life Sciences, Dundee DD1 5EH, UK
    EMBO Rep 8:332-4. 2007
  3. pmc Replication licensing and cancer--a fatal entanglement?
    J Julian Blow
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, DD1 5EH, UK
    Nat Rev Cancer 8:799-806. 2008
  4. pmc Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins
    Peter J Gillespie
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, UK
    Methods 57:203-13. 2012
  5. pmc Deregulated replication licensing causes DNA fragmentation consistent with head-to-tail fork collision
    Iain F Davidson
    School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell 24:433-43. 2006
  6. pmc Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition
    Guennadi A Khoudoli
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, United Kingdom
    Curr Biol 18:838-43. 2008
  7. pmc ELYS/MEL-28 chromatin association coordinates nuclear pore complex assembly and replication licensing
    Peter J Gillespie
    Division of Gene Regulation and Expression, College of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    Curr Biol 17:1657-62. 2007
  8. pmc A Xenopus Dbf4 homolog is required for Cdc7 chromatin binding and DNA replication
    Pedro Jares
    Wellcome Trust Biocentre, University of Dundee, DD1 5EH, UK
    BMC Mol Biol 5:5. 2004
  9. pmc A model for DNA replication showing how dormant origins safeguard against replication fork failure
    J Julian Blow
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    EMBO Rep 10:406-12. 2009
  10. pmc Replication licensing--defining the proliferative state?
    J Julian Blow
    Cancer Research Campaign CRC Chromosome Replication Research Group, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee, UK DD1 5EH
    Trends Cell Biol 12:72-8. 2002

Collaborators

Detail Information

Publications42

  1. pmc Mammalian nuclei become licensed for DNA replication during late telophase
    Daniela S Dimitrova
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    J Cell Sci 115:51-9. 2002
    ..We conclude that the functional association of Mcm proteins with chromatin (i.e. replication licensing) in CHO cells takes place during telophase, several hours prior to the specification of replication origins at the DHFR locus...
  2. pmc The elusive determinants of replication origins
    Silvia Costa
    Division of Gene Regulation and Expression, College of Life Sciences, Dundee DD1 5EH, UK
    EMBO Rep 8:332-4. 2007
  3. pmc Replication licensing and cancer--a fatal entanglement?
    J Julian Blow
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, DD1 5EH, UK
    Nat Rev Cancer 8:799-806. 2008
    ..This misregulation can cause either under- or over-replication of chromosomal DNA, and could explain the genetic instability commonly seen in cancer cells...
  4. pmc Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins
    Peter J Gillespie
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, UK
    Methods 57:203-13. 2012
    ..These recently developed and revised techniques provide a practical starting point for investigating the function of proteins involved in DNA replication...
  5. pmc Deregulated replication licensing causes DNA fragmentation consistent with head-to-tail fork collision
    Iain F Davidson
    School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell 24:433-43. 2006
    ..The unusual characteristics of these fragments suggest that they result from head-to-tail collision (rear ending) of replication forks chasing one another along the same DNA template...
  6. pmc Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition
    Guennadi A Khoudoli
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, United Kingdom
    Curr Biol 18:838-43. 2008
    ....
  7. pmc ELYS/MEL-28 chromatin association coordinates nuclear pore complex assembly and replication licensing
    Peter J Gillespie
    Division of Gene Regulation and Expression, College of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    Curr Biol 17:1657-62. 2007
    ..Because nuclear assembly is required to shut down licensing prior to entry into S phase, our results suggest a mechanism by which these two early cell-cycle events are coordinated with one another...
  8. pmc A Xenopus Dbf4 homolog is required for Cdc7 chromatin binding and DNA replication
    Pedro Jares
    Wellcome Trust Biocentre, University of Dundee, DD1 5EH, UK
    BMC Mol Biol 5:5. 2004
    ..Licensed origins must then be activated by S phase-inducing cyclin-dependent kinases (S-CDKs) and the Dbf4/Cdc7 kinase...
  9. pmc A model for DNA replication showing how dormant origins safeguard against replication fork failure
    J Julian Blow
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    EMBO Rep 10:406-12. 2009
    ..This provides a simple explanation for how replication origin firing is regulated, which simultaneously provides protection against replicative stress while minimizing the cost of using large numbers of replication forks...
  10. pmc Replication licensing--defining the proliferative state?
    J Julian Blow
    Cancer Research Campaign CRC Chromosome Replication Research Group, Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee, UK DD1 5EH
    Trends Cell Biol 12:72-8. 2002
    ..These results have prompted us to propose a functional distinction between the proliferative state and the non-proliferative state (including G0) depending on whether origins are licensed...
  11. pmc How dormant origins promote complete genome replication
    J Julian Blow
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee Dow Street, Dundee DD1 5EH, UK
    Trends Biochem Sci 36:405-14. 2011
    ..We review the function of dormant origins, the molecular mechanism of their regulation and their physiological implications...
  12. pmc Replication forks, chromatin loops and dormant replication origins
    J Julian Blow
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Genome Biol 9:244. 2008
    ..Recent work demonstrates that cells adapt by changing the organization of chromatin loops and maintaining the new pattern of origin use in subsequent cell cycles...
  13. pmc The chromosome cycle: coordinating replication and segregation. Second in the cycles review series
    J Julian Blow
    University of Dundee, Wellcome Trust Biocentre, Dow Street, Dundee DD1 5EH, UK
    EMBO Rep 6:1028-34. 2005
    ..Here we provide a general overview of how these two systems are coordinated to maintain genetic stability during the cell cycle...
  14. pmc The Cdc7/Dbf4 protein kinase: target of the S phase checkpoint?
    P Jares
    Department of Biochemistry, University of Dundee, UK
    EMBO Rep 1:319-22. 2000
    ..In this review we attempt to bring together new information to explain how Cdc7/Dbf4 may perform these two distinct functions...
  15. pmc Replication origins in Xenopus egg extract Are 5-15 kilobases apart and are activated in clusters that fire at different times
    J J Blow
    Cancer Research Campaign, Chromosome Replication Research Group, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Cell Biol 152:15-25. 2001
    ..This suggests that the binding of ORCs to chromatin determines the regular spacing of origins in this system...
  16. pmc Preventing re-replication of chromosomal DNA
    J Julian Blow
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    Nat Rev Mol Cell Biol 6:476-86. 2005
    ..Recent data have provided biochemical and structural insight into the process of replication licensing and the mechanisms that regulate it during the cell cycle...
  17. pmc Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins
    P J Gillespie
    CRC Chromosome Replication Research Group, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    BMC Biochem 2:15. 2001
    ..Mcm2-7 are essential for DNA replication, but are displaced from origins as they initiate, thus ensuring that no origin fires more than once in a single cell cycle...
  18. pmc Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin
    S Tada
    CRC Chromosome Replication Research Group, Wellcome Trust Biocentre, Dow Street University of Dundee, Dundee DD1 5EH, UK
    Nat Cell Biol 3:107-13. 2001
    ..These experiments suggest that geminin-mediated inhibition of RLF-B/Cdt1 is essential for repressing origin assembly late in the cell cycle of higher eukaryotes...
  19. ncbi request reprint The regulation of replication origin activation
    A D Donaldson
    CRC Chromosome Replication Group, Wellcome Trust Building, University of Dundee, Dundee DD1 5EH, UK
    Curr Opin Genet Dev 9:62-8. 1999
    ..These processes are key targets of cell-cycle control, and understanding their regulation will provide a deeper knowledge of the mechanisms controlling cell proliferation...
  20. pmc The role of Cdc6 in ensuring complete genome licensing and S phase checkpoint activation
    Maren Oehlmann
    Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    J Cell Biol 165:181-90. 2004
    ..These results show that Cdc6 plays multiple roles in ensuring precise chromosome duplication...
  21. pmc Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination
    Anatoliy Li
    Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Nat Cell Biol 6:260-7. 2004
    ..This reveals an unexpected role for CDKs and ubiquitination in activating chromosomal DNA replication...
  22. ncbi request reprint Use of peptides from p21 (Waf1/Cip1) to investigate PCNA function in Xenopus egg extracts
    H Mattock
    Department of Surgery and Molecular Oncology, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, United Kingdom
    Exp Cell Res 265:242-51. 2001
    ..However, systems for analyzing the far more complex mechanisms required for the replication of nuclear double-stranded DNA have proved so far to be refractory to specific PCNA depletion...
  23. pmc Cell type-specific responses of human cells to inhibition of replication licensing
    S Shreeram
    Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Oncogene 21:6624-32. 2002
    ..These results suggest that inhibition of the licensing system may cause cancer-specific cell killing and therefore represent a novel anti-cancer target...
  24. pmc Excess Mcm2-7 license dormant origins of replication that can be used under conditions of replicative stress
    Anna M Woodward
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 173:673-83. 2006
    ....
  25. pmc Negative regulation of geminin by CDK-dependent ubiquitination controls replication licensing
    Anatoliy Li
    Wellcome Trust Biocentre, University of Dundee, Dundee, Scotland, UK
    Cell Cycle 3:443-5. 2004
    ..These results suggest a simple model for how precise chromosome duplication is ensured in the Xenopus model system...
  26. pmc DNA replication: stable driving prevents fatal smashes
    A D Donaldson
    CRC Chromosome Replication Research Group, Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland
    Curr Biol 11:R979-82. 2001
    ..Cells respond to DNA damage during S phase by slowing chromosome replication. Recent results have shed light on the mechanism by which this 'intra-S phase' checkpoint is implemented...
  27. pmc A new role for Ran in ensuring precise duplication of chromosomal DNA
    J Julian Blow
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, Scotland
    Cell 113:2-4. 2003
    ..New work by Yamaguchi et al. (in this issue of Cell) shows that the small GTPase Ran can directly inhibit licensing inside nuclei once CDKs are active late in the cell cycle...
  28. pmc Characterization of a novel ATR-dependent, Chk1-independent, intra-S-phase checkpoint that suppresses initiation of replication in Xenopus
    M Gloria Luciani
    Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee, DD1 5EH, UK
    J Cell Sci 117:6019-30. 2004
    ..This suggests that special mechanisms might be necessary to reverse the effects of the intra-S-phase checkpoint once it has acted on particular origins...
  29. pmc The role of the replication licensing system in cell proliferation and cancer
    S Shreeram
    Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Prog Cell Cycle Res 5:287-93. 2003
    ..We also review how detection of licensing components can be used for the diagnosis and prognosis of cancer. Finally we discuss the potential of the replication licensing system as a novel anti-cancer target...
  30. doi request reprint PTIP/Swift is required for efficient PCNA ubiquitination in response to DNA damage
    Thomas Gohler
    College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    DNA Repair (Amst) 7:775-87. 2008
    ..Our results suggest that PTIP/Swift is an important new regulator of DNA damage avoidance in metazoans...
  31. pmc Geminin becomes activated as an inhibitor of Cdt1/RLF-B following nuclear import
    Ben Hodgson
    Cancer Research UK Chromosome Replication, Research Group, Wellcome Trust Biocentre, University of Dundee, Dow Street, DD1 5EH, Dundee, United Kingdom
    Curr Biol 12:678-83. 2002
    ..Since the initiation of replication at licensed origins depends on nuclear assembly, our results suggest an elegant and novel mechanism for preventing rereplication of DNA in a single cell cycle...
  32. pmc Live-cell imaging reveals replication of individual replicons in eukaryotic replication factories
    Etsushi Kitamura
    School of Life Sciences, University of Dundee, Wellcome Trust Biocentre, Dow Street, Dundee, UK
    Cell 125:1297-308. 2006
    ..This assay system allows replication to be studied at extremely high temporal resolution in individual cells, thereby opening a window into how replication dynamics vary from cell to cell...
  33. pmc Replication factory activation can be decoupled from the replication timing program by modulating Cdk levels
    Alexander M Thomson
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 188:209-21. 2010
    ..The ability of Cdks to differentially effect replication initiation, factory activation, and progression through the timing program provides new insights into the way that chromosomal DNA replication is organized during S phase...
  34. pmc Dormant origins licensed by excess Mcm2-7 are required for human cells to survive replicative stress
    Xin Quan Ge
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom
    Genes Dev 21:3331-41. 2007
    ..We propose that checkpoint kinase activity can preferentially suppress initiation within inactive replicon clusters, thereby directing new initiation events toward active clusters that are experiencing replication problems...
  35. pmc Histone acetylation by HBO1 tightens replication licensing
    Gaganmeet Singh Chadha
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Mol Cell 37:5-6. 2010
    ....
  36. pmc Cdt1 downregulation by proteolysis and geminin inhibition prevents DNA re-replication in Xenopus
    Anatoliy Li
    Wellcome Trust Biocentre, University of Dundee, Dundee, UK
    EMBO J 24:395-404. 2005
    ..The results also explain the original experiments that led to the proposal of a replication licensing factor...
  37. pmc Bod1, a novel kinetochore protein required for chromosome biorientation
    Iain M Porter
    Division of Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    J Cell Biol 179:187-97. 2007
    ..Therefore, Bod1 is a novel kinetochore protein that is required for the detection or resolution of syntelic attachments in mitotic spindles...
  38. pmc Functional domains of the Xenopus replication licensing factor Cdt1
    Andrew Ferenbach
    Wellcome Trust Biocentre, University of Dundee Dow Street, Dundee DD1 5EH, UK
    Nucleic Acids Res 33:316-24. 2005
    ..Separation of the domains necessary for licensing activity from domains required for a strong interaction with geminin generated a construct, whose licensing activity was partially insensitive to geminin inhibition...
  39. ncbi request reprint DNA replication licensing in somatic and germ cells
    Kathryn Leigh Eward
    Wolfson Institute for Biomedical Research, University College London, The Cruciform Building, Gower Street, London, WC1E 6BT, UK
    J Cell Sci 117:5875-86. 2004
    ....
  40. ncbi request reprint Regulating the licensing of DNA replication origins in metazoa
    Melvin L DePamphilis
    National Institute of Child Health and Human Development, National Institutes of Health, Building 6 3A 15, 9000 Rockville Pike, Bethesda, MD 20892 2753, USA
    Curr Opin Cell Biol 18:231-9. 2006
    ....
  41. pmc The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription
    Conrad A Nieduszynski
    Institute of Medical Sciences, University of Aberdeen Foresterhill, Aberdeen AB25 2ZD, Scotland, UK
    Nucleic Acids Res 33:2410-20. 2005
    ..This hierarchy is both 'hard-wired' by the minimal origin sequences and 'soft-wired' by local transcriptional context...
  42. pmc Degradation ensures integrity
    Anatoliy Li
    Nature 423:818-9. 2003