B C Berks

Summary

Affiliation: University of East Anglia
Country: UK

Publications

  1. ncbi A common export pathway for proteins binding complex redox cofactors?
    B C Berks
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK
    Mol Microbiol 22:393-404. 1996
  2. ncbi A novel protein transport system involved in the biogenesis of bacterial electron transfer chains
    B C Berks
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK
    Biochim Biophys Acta 1459:325-30. 2000
  3. ncbi The Tat protein export pathway
    B C Berks
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Mol Microbiol 35:260-74. 2000
  4. ncbi Membrane interactions and self-association of the TatA and TatB components of the twin-arginine translocation pathway
    E De Leeuw
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK
    FEBS Lett 506:143-8. 2001
  5. ncbi Purified components of the Escherichia coli Tat protein transport system form a double-layered ring structure
    F Sargent
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK
    Eur J Biochem 268:3361-7. 2001
  6. ncbi TatD is a cytoplasmic protein with DNase activity. No requirement for TatD family proteins in sec-independent protein export
    M Wexler
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 275:16717-22. 2000
  7. ncbi The twin arginine consensus motif of Tat signal peptides is involved in Sec-independent protein targeting in Escherichia coli
    N R Stanley
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 275:11591-6. 2000
  8. pmc Escherichia coli strains blocked in Tat-dependent protein export exhibit pleiotropic defects in the cell envelope
    N R Stanley
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Bacteriol 183:139-44. 2001
  9. pmc Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth
    R L Jack
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Bacteriol 183:1801-4. 2001
  10. ncbi Novel heme ligation in a c-type cytochrome involved in thiosulfate oxidation: EPR and MCD of SoxAX from Rhodovulum sulfidophilum
    M R Cheesman
    School of Chemical Sciences, Centre for Metalloprotein Spectroscopy and Biology, University of East Anglia, Norwich NR4 7TJ, UK
    Biochemistry 40:10562-9. 2001

Collaborators

Detail Information

Publications21

  1. ncbi A common export pathway for proteins binding complex redox cofactors?
    B C Berks
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK
    Mol Microbiol 22:393-404. 1996
    ..The nature of this specialization, the structure of the double-arginine signal sequences, and the possible relationship with the double-arginine signal peptide-dependent thylakoid import pathway are discussed...
  2. ncbi A novel protein transport system involved in the biogenesis of bacterial electron transfer chains
    B C Berks
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK
    Biochim Biophys Acta 1459:325-30. 2000
    ..Here we review the operation of the bacterial Tat system and propose a model for the structural organisation of the Tat preprotein translocase...
  3. ncbi The Tat protein export pathway
    B C Berks
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Mol Microbiol 35:260-74. 2000
    ..Here, we review recent progress on the characterization of the Tat system and critically discuss the structure and operation of this major new bacterial protein export pathway...
  4. ncbi Membrane interactions and self-association of the TatA and TatB components of the twin-arginine translocation pathway
    E De Leeuw
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK
    FEBS Lett 506:143-8. 2001
    ..The presence of such homo-oligomeric interactions is supported by size exclusion chromatography...
  5. ncbi Purified components of the Escherichia coli Tat protein transport system form a double-layered ring structure
    F Sargent
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, UK
    Eur J Biochem 268:3361-7. 2001
    ..Negative stain electron microscopy of the complex reveals cylindrical structures that may correspond to the Tat protein transport channel...
  6. ncbi TatD is a cytoplasmic protein with DNase activity. No requirement for TatD family proteins in sec-independent protein export
    M Wexler
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 275:16717-22. 2000
    ..TatD is shown to be a cytoplasmic protein. TatD binds to immobilized Ni(2+) or Zn(2+) affinity columns and exhibits magnesium-dependent DNase activity. Features of the tatA operon that may control TatD expression are discussed...
  7. ncbi The twin arginine consensus motif of Tat signal peptides is involved in Sec-independent protein targeting in Escherichia coli
    N R Stanley
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 275:11591-6. 2000
    ..Tat signal peptides characteristically have positively charged carboxyl-terminal regions. However, changing the sign of this charge does not affect export of SufI...
  8. pmc Escherichia coli strains blocked in Tat-dependent protein export exhibit pleiotropic defects in the cell envelope
    N R Stanley
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Bacteriol 183:139-44. 2001
    ..Both phenotypes are similar to those displayed by strains carrying point mutations in the lpxC (envA) gene. The phenotype was not replicated by mutations affecting synthesis and/or activity of all known or predicted Tat substrates...
  9. pmc Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth
    R L Jack
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Bacteriol 183:1801-4. 2001
    ..Expression studies indicate that the tatABCD and tatE transcription units are expressed constitutively. Translational fusion experiments suggest that TatA is synthesized at a much higher level than the other Tat proteins...
  10. ncbi Novel heme ligation in a c-type cytochrome involved in thiosulfate oxidation: EPR and MCD of SoxAX from Rhodovulum sulfidophilum
    M R Cheesman
    School of Chemical Sciences, Centre for Metalloprotein Spectroscopy and Biology, University of East Anglia, Norwich NR4 7TJ, UK
    Biochemistry 40:10562-9. 2001
    ..Sequence analysis places the histidine/methionine-coordinated heme in SoxX and the thiolate-liganded hemes in SoxA. SoxAX is the first naturally occurring c-type cytochrome in which a thiolate-coordinated heme has been identified...
  11. ncbi A naturally occurring bacterial Tat signal peptide lacking one of the 'invariant' arginine residues of the consensus targeting motif
    A P Hinsley
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 497:45-9. 2001
    ..The possible implications of this finding for the assembly of electron transfer complexes containing Rieske proteins in plant organelles are discussed...
  12. pmc Structural investigation of the molybdenum site of the periplasmic nitrate reductase from Thiosphaera pantotropha by X-ray absorption spectroscopy
    B Bennett
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, University Plain, Norwich, UK
    Biochem J 317:557-63. 1996
    ..The periplasmic nitrate reductase molybdenum co-ordination environment in both the Mo(VI) and Mo(IV) oxidation states is distinct from that found in the membrane-bound respiratory nitrate reductase...
  13. ncbi Functional, biochemical and genetic diversity of prokaryotic nitrate reductases
    D J Richardson
    The Centre for Metalloprotein Spectroscopy and Biology, Schools of Biological Sciences and Chemical Sciences, University of East Anglia, Norwich, United Kingdom
    Cell Mol Life Sci 58:165-78. 2001
    ..Analysis of prokaryotic genome sequences available at the time of writing reveals that the different nitrate reductases are phylogenetically widespread...
  14. ncbi Sec-independent protein translocation in Escherichia coli. A distinct and pivotal role for the TatB protein
    F Sargent
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Biol Chem 274:36073-82. 1999
    ..coli tatA mutant, but not a tatB mutant. Analysis of Tat component stability in various tat deletion backgrounds shows that TatC is rapidly degraded in the absence of TatB suggesting that TatC complexes, and is stabilized by, TatB...
  15. pmc Cytochrome complex essential for photosynthetic oxidation of both thiosulfate and sulfide in Rhodovulum sulfidophilum
    C Appia-Ayme
    Center for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    J Bacteriol 183:6107-18. 2001
    ..Gene clusters similar to the R. sulfidophilum sox locus can be found in the genome of a green sulfur bacterium and in phylogenetically diverse nonphotosynthetic autotrophs...
  16. ncbi Novel domain packing in the crystal structure of a thiosulphate-oxidizing enzyme
    V A Bamford
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Biochem Soc Trans 30:638-42. 2002
    ..The packing of c-type cytochrome domains both within SoxA and at the interface between the subunits of the complex has been compared with other examples and found to be unique...
  17. ncbi Assignment of haem ligands and detection of electronic absorption bands of molybdenum in the di-haem periplasmic nitrate reductase of Paracoccus pantotrophus
    C S Butler
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological and Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    FEBS Lett 500:71-4. 2001
    ..The low-temperature MCD spectrum shows oppositely signed bands in this region (peak 648 nm, trough 714 nm) which have been assigned to S-to-Mo(V) charge transfer transitions...
  18. ncbi An essential component of a novel bacterial protein export system with homologues in plastids and mitochondria
    E G Bogsch
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Biol Chem 273:18003-6. 1998
    ..These findings suggest a central role for TatC-type proteins in the translocation of tightly folded proteins across a spectrum of biological membranes...
  19. ncbi A genetic screen for suppressors of Escherichia coli Tat signal peptide mutations establishes a critical role for the second arginine within the twin-arginine motif
    G Buchanan
    Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
    Arch Microbiol 177:107-12. 2001
    ..The growth phenotype of the mutant strains was exploited in a genetic screen with the aim of identifying second-site suppressor mutations that allowed export of the modified TorA precursors...
  20. pmc Overlapping functions of components of a bacterial Sec-independent protein export pathway
    F Sargent
    Nitrogen Fixation Laboratory, John Innes Centre, Colney, Norwich NR4 7UH
    EMBO J 17:3640-50. 1998
    ..A mutation previously assigned to the hcf106 homologue encoded at the tatABCD locus, mttA, lies instead in the tatB gene...
  21. ncbi A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system
    S C Andrews
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Microbiology 143:3633-47. 1997
    ..It is likely that HyfR acts as a formate-dependent regulator of the hyf operon and that FocB provides the Hyf complex with external formate as substrate...