J D Beggs

Summary

Affiliation: University of Edinburgh
Country: UK

Publications

  1. pmc A splicing-dependent transcriptional checkpoint associated with prespliceosome formation
    Keerthi T Chathoth
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Mol Cell 53:779-90. 2014
  2. pmc Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast
    Martin A M Reijns
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, UK
    FEBS J 276:3602-17. 2009
  3. pmc The Lsm2-8 complex determines nuclear localization of the spliceosomal U6 snRNA
    Michael P Spiller
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 35:923-9. 2007
  4. pmc Splicing-dependent RNA polymerase pausing in yeast
    Ross D Alexander
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Edinburgh EH9 3JR, UK
    Mol Cell 40:582-93. 2010
  5. ncbi request reprint Lsm proteins and RNA processing
    J D Beggs
    Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, U K
    Biochem Soc Trans 33:433-8. 2005
  6. pmc Extensive interactions of PRP8 protein with the 5' and 3' splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA
    S Teigelkamp
    Institute of Cell and Molecular Biology, University of Edinburgh, Cambridge, UK
    EMBO J 14:2602-12. 1995
  7. pmc Roles of PRP8 protein in the assembly of splicing complexes
    J D Brown
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    EMBO J 11:3721-9. 1992
  8. pmc Interaction of the yeast splicing factor PRP8 with substrate RNA during both steps of splicing
    S Teigelkamp
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nucleic Acids Res 23:320-6. 1995
  9. pmc The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA
    S Teigelkamp
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    EMBO J 13:888-97. 1994
  10. ncbi request reprint The role of PRP8 protein in nuclear pre-mRNA splicing in yeast
    J D Beggs
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    J Cell Sci Suppl 19:101-5. 1995

Collaborators

  • A J Newman
  • P Legrain
  • J D Brown
  • M Kupiec
  • Kum Loong Boon
  • Martin A M Reijns
  • Keerthi T Chathoth
  • J David Barrass
  • Ross D Alexander
  • I Dix
  • Michael P Spiller
  • S Ben-Yehuda
  • C S Russell
  • S Teigelkamp
  • A E Mayes
  • Shaun Webb
  • R W van Nues
  • S Tharun
  • Steven A Innocente
  • Tatsiana Auchynnikava
  • M Plumpton
  • P Lennertz
  • R Parker
  • W He
  • L Verdone
  • S B Yehuda
  • C Russell
  • M McGarvey
  • E Whittaker
  • D J Jamieson
  • S P Bjørn
  • D S King
  • T Paterson
  • G J Anderson
  • R Luhrmann
  • A Soltyk
  • J Pringle
  • B Rahe
  • D J Finnegan
  • M Bach
  • J D Friesen

Detail Information

Publications23

  1. pmc A splicing-dependent transcriptional checkpoint associated with prespliceosome formation
    Keerthi T Chathoth
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Mol Cell 53:779-90. 2014
    ..We propose a role for Cus2p as a potential checkpoint factor in transcription...
  2. pmc Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast
    Martin A M Reijns
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, UK
    FEBS J 276:3602-17. 2009
    ..These findings contribute to our understanding of how two very similar protein complexes can have different properties...
  3. pmc The Lsm2-8 complex determines nuclear localization of the spliceosomal U6 snRNA
    Michael P Spiller
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Nucleic Acids Res 35:923-9. 2007
    ..La protein, which binds only transiently to the nascent U6 transcript, has a smaller, apparently indirect, effect on U6 localization that is compatible with its proposed role as a chaperone in facilitating U6 snRNP assembly...
  4. pmc Splicing-dependent RNA polymerase pausing in yeast
    Ross D Alexander
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Edinburgh EH9 3JR, UK
    Mol Cell 40:582-93. 2010
    ..Phosphorylated polymerase also accumulates around the 3' splice sites of constitutively expressed, endogenous yeast genes. We propose that transcriptional pausing is imposed by a checkpoint associated with cotranscriptional splicing...
  5. ncbi request reprint Lsm proteins and RNA processing
    J D Beggs
    Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, U K
    Biochem Soc Trans 33:433-8. 2005
    ..This article reviews the properties of the Sm and Lsm proteins and structurally and functionally related proteins in archaea and eubacteria...
  6. pmc Extensive interactions of PRP8 protein with the 5' and 3' splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA
    S Teigelkamp
    Institute of Cell and Molecular Biology, University of Edinburgh, Cambridge, UK
    EMBO J 14:2602-12. 1995
    ..We propose that the large binding sites of PRP8 stabilize the intrinsically weaker interactions of U5 snRNA with both exons at the splice sites for exon alignment by the U5 snRNP...
  7. pmc Roles of PRP8 protein in the assembly of splicing complexes
    J D Brown
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    EMBO J 11:3721-9. 1992
    ....
  8. pmc Interaction of the yeast splicing factor PRP8 with substrate RNA during both steps of splicing
    S Teigelkamp
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nucleic Acids Res 23:320-6. 1995
    ..The results allow a first glimpse at the pattern of PRP8 protein-RNA interactions during splicing and provide a fundamental basis for future analysis of these interactions...
  9. pmc The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA
    S Teigelkamp
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    EMBO J 13:888-97. 1994
    ..Since PRP2 is a putative RNA helicase, we propose that the pre-mRNA is the target of RNA displacement activity of PRP2 protein, promoting the first step of splicing...
  10. ncbi request reprint The role of PRP8 protein in nuclear pre-mRNA splicing in yeast
    J D Beggs
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    J Cell Sci Suppl 19:101-5. 1995
    ..It is proposed that PRP8 protein may stabilize fragile interactions between the U5 snRNA and exon sequences at the splice sites, to anchor and align them in the catalytic centre of the spliceosome...
  11. pmc Functional contacts with a range of splicing proteins suggest a central role for Brr2p in the dynamic control of the order of events in spliceosomes of Saccharomyces cerevisiae
    R W van Nues
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Genetics 157:1451-67. 2001
    ..Overall, these protein interaction studies shed light on how splicing factors regulate the order of events in the large spliceosome complex...
  12. ncbi request reprint A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase
    D J Jamieson
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nature 349:715-7. 1991
    ..The possible role of this putative helicase in nuclear pre-mRNA splicing is discussed...
  13. pmc A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes
    M Plumpton
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    EMBO J 13:879-87. 1994
    ..As ATPase activity was reduced only slightly, but splicing activity was abolished, we propose that the dominant negative phenotype is due primarily to a defect in the putative RNA helicase activity of PRP2 protein...
  14. pmc Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae
    D S King
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nucleic Acids Res 18:6559-64. 1990
    ..By elution of electrophoretically distinct spliceosomal complexes from non-denaturing gels we identify the specific complex with which PRP2 initially interacts in the pathway of spliceosome assembly...
  15. pmc Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae
    C S Russell
    The Wellcome Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, UK
    RNA 6:1565-72. 2000
    ..We discuss the likelihood that Syf1p, Syf2p, and Syf3p are components of a protein complex that assembles into spliceosomes and also regulates cell cycle progression...
  16. pmc Characterization of Sm-like proteins in yeast and their association with U6 snRNA
    A E Mayes
    Institute of Cell and Molecular Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    EMBO J 18:4321-31. 1999
    ..We discuss the possible existence and functions of alternative Lsm complexes, including the likelihood that they are involved in processes other than pre-mRNA splicing...
  17. ncbi request reprint Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein
    G J Anderson
    Department of Molecular Biology, University of Edinburgh, UK
    Nature 342:819-21. 1989
    ..The HeLa cell protein has an Mr greater than 200K and is associated with purified 20S U5 snRNPs. This is the first report of phylogenetic conservation between yeast and man of a protein splicing factor...
  18. ncbi request reprint A suppressor of yeast spp81/ded1 mutations encodes a very similar putative ATP-dependent RNA helicase
    D J Jamieson
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Mol Microbiol 5:805-12. 1991
    ..The nucleotide sequence of the DBP1 gene revealed that it also encoded a putative ATP-dependent RNA helicase which showed considerable similarity at the amino acid level to the SPP81/DED1 protein...
  19. pmc Extensive genetic interactions between PRP8 and PRP17/CDC40, two yeast genes involved in pre-mRNA splicing and cell cycle progression
    S Ben-Yehuda
    Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel
    Genetics 154:61-71. 2000
    ..On the basis of these findings, we propose a model for the mode of interaction between the Prp8 and Prp17 proteins during the second catalytic step of the splicing reaction...
  20. pmc The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae
    I Dix
    Institute of Cell and Molecular Biology, University of Edinburgh, United Kingdom
    RNA 5:360-8. 1999
    ..Using a sensitive in vivo splicing assay, however, we observed lower splicing efficiency in the isy1 null mutant compared to wild-type, indicating that Isy1 p is important in the optimization of splicing...
  21. pmc PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle
    S P Bjørn
    Hospital for Sick Children, Toronto, Ontario, Canada
    Mol Cell Biol 9:3698-709. 1989
    ..Immunoprecipitations with the antibodies indicated that the PRP4 protein is associated with the U4/U6 small nuclear ribonucleoprotein particle...
  22. pmc Polypeptide components of Drosophila small nuclear ribonucleoprotein particles
    T Paterson
    Institute of Cell and Molecular Biology, University of Edinburgh
    Nucleic Acids Res 19:5877-82. 1991
    ....
  23. ncbi request reprint Yeast Sm-like proteins function in mRNA decapping and decay
    S Tharun
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson 85721, USA
    Nature 404:515-8. 2000
    ..In addition, the Lsm complex that functions in mRNA decay appears to be distinct from the U6-associated Lsm complex, indicating that Lsm proteins form specific complexes that affect different aspects of mRNA metabolism...