Genomes and Genes
Affiliation: University College London
- Winging it--actin on the flyBuzz Baum
LICR, Department of Biochemistry and Molecular Biology, University College London, Gower Street, WC1E 6BT, London, United Kingdom
Dev Cell 2:125-6. 2002..New insights may be gained from the recent identification of a conserved cofilin phosphatase, Slingshot, which modulates actin dynamics to help control Drosophila wing hair morphogenesis...
- Animal development: crowd controlBuzz Baum
Ludwig Institute for Cancer Research, University College London Branch, 91 Riding House Street, London W1W 7BS, UK
Curr Biol 14:R716-8. 2004..Two recent studies help show how this is achieved during convergence and extension of the Drosophila germ-band, where polarity within the plane of the embryonic epithelium biases junction remodeling to polarize cell intercalation...
- Actin nucleation: spire - actin nucleator in a class of its ownBuzz Baum
Ludwig Institute for Cancer Research, UCL Branch, London, UK
Curr Biol 15:R305-8. 2005..A recent study has now identified in Spire a third class of actin nucleator. The four short WH2 repeats within Spire bind four consecutive actin monomers to form a novel single strand nucleus for 'barbed end' actin filament elongation...
- Moesin controls cortical rigidity, cell rounding, and spindle morphogenesis during mitosisPatricia Kunda
MRC Laboratory of Molecular Cell Biology, UCL, Gower Street, London WC1E 6BT, United Kingdom
Curr Biol 18:91-101. 2008..Here we identify Moesin as a key player. Moesin is the sole Drosophila member of the ERM proteins, which, once activated via phosphorylation, crosslink actin filaments to the cytoplasmic tails of plasma membrane proteins...
- Left-right asymmetry: actin-myosin through the looking glassBuzz Baum
Ludwig Institute for Cancer Research, 91 Riding House Street, London W1W 7BS, UK
Curr Biol 16:R502-4. 2006..In several animals, microtubule-based structures are thought to be the source of chiral information used to establish handedness. Now, two new studies in Drosophila identify a role for unconventional myosin motors in this process...
- RNAi in a postmodern, postgenomic eraBuzz Baum
Ludwig Institute for Cancer Research, UCL Branch, London W1W 7BS, UK
Oncogene 23:8336-9. 2004....
- Polarity proteins and Rho GTPases cooperate to spatially organise epithelial actin-based protrusionsMarios Georgiou
MRC Laboratory of Molecular Cell Biology, UCL, London, WC1E 6BT, UK
J Cell Sci 123:1089-98. 2010....
- Dynamic filopodia transmit intermittent Delta-Notch signaling to drive pattern refinement during lateral inhibitionMichael Cohen
Centre for Mathematics and Physics in the Life Sciences and Experimental Biology, London, WC1E 6BT, UK
Dev Cell 19:78-89. 2010..Significantly, the intermittent signaling induced by these filopodial dynamics generates a type of structured noise that is uniquely suited to the generation of well-ordered, tissue-wide epithelial patterns...
- The FLIGHT Drosophila RNAi database: 2010 updateDavid Sims
Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, UK
Fly (Austin) 4:344-8. 2010..FLIGHT integrates RNAi screen data with microarray gene expression as well as genomic annotations and genetic/physical interaction datasets to provide a single interface for RNAi screen analysis and data-mining in Drosophila...
- Tao-1 is a negative regulator of microtubule plus-end growthTao Liu
MRC Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK
J Cell Sci 123:2708-16. 2010..These data reveal a role for Tao-1 in controlling the dynamic interplay between microtubule plus ends and the actin cortex in the regulation of cell form...
- Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphologyTao Liu
MRC Laboratory of Molecular Cell Biology, UCL, London, UK
Genome Biol 10:R26. 2009..However, to date, most RNAi screens have been performed in a single cell line, and results then extrapolated across cell types and systems...
- The importance of structured noise in the generation of self-organizing tissue patterns through contact-mediated cell-cell signallingMichael Cohen
CoMPLEX, University College London, London, UK
J R Soc Interface 8:787-98. 2011..Thus, intermittent protrusion-based signalling has the potential to yield robust self-organizing tissue-wide patterns without the need to invoke diffusion-mediated signalling...
- Dynamic cofilin phosphorylation in the control of lamellipodial actin homeostasisEleonora Jovceva
Ludwig Institute for Cancer Research, UCL Branch, London, W1W 7BS, UK
J Cell Sci 120:1888-97. 2007....
- PDGF/VEGF signaling controls cell size in DrosophilaDavid Sims
Morphogenesis Group, Ludwig Institute for Cancer Research UCL Branch, Riding House Street, London, W1W 7BS, UK
Genome Biol 10:R20. 2009..To delineate the pathways controlling this process, we screened a genome-scale, image-based Drosophila RNA interference dataset for double-stranded RNAs that reduce the average size of adherent S2R+ cells...
- Abi, Sra1, and Kette control the stability and localization of SCAR/WAVE to regulate the formation of actin-based protrusionsPatricia Kunda
Ludwig Institute for Cancer Research University College Branch, London, UK
Curr Biol 13:1867-75. 2003..SCAR and WASP then activate the Arp2/3 complex to nucleate the formation of new actin filaments, which through polymerization exert a protrusive force on the membrane...
- Drosophila oogenesis: generating an axis of polarityBuzz Baum
Ludwig Institute for Cancer Research, University College London Branch, 91 Riding House Street, London W1W 7BS, UK
Curr Biol 12:R835-7. 2002..New studies of moesin function during Drosophila oogenesis show that Dmoesin tethers actin to the oocyte membrane. Interestingly, Dmoesin and an intact cortex are also required to stabilise ooycte polarity...
- The metastatic cancer cell cortex: an adaptation to enhance robust cell division in novel environments?Helen K Matthews
MRC Laboratory for Molecular Cell Biology, University College London, London, UK
Bioessays 34:1017-20. 2012..We suggest that mitotic cell rounding, aided by several actin-regulatory oncogenes, may facilitate this process in a robust, context-independent manner...
- Myosin II-dependent cortical movement is required for centrosome separation and positioning during mitotic spindle assemblyJody Rosenblatt
MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London SC1E 6BT, United Kingdom
Cell 117:361-72. 2004..We propose that after NEBD, completion of centrosome separation and positioning around chromosomes depends on astral microtubule connections to a moving cell cortex...
- The actin cytoskeleton in spindle assembly and positioningPatricia Kunda
Department of Cell and Developmental Biology, University College London, UK
Trends Cell Biol 19:174-9. 2009..Here, we discuss this recent work and the possible role of crosstalk between the mitotic actin cortex and the plus ends of astral microtubules in this process...
- Cell shape: taking the heatPatricia Kunda
MRC Laboratory for Molecular Cell Biology, and the Department of Cell and Developmental Biology, UCL, Gower Street, London, UK
Curr Biol 18:R470-2. 2008..A new study reveals how an extracellular protein--Spam--helps mechanosensory organs in the fruit fly to withstand the effects of the water loss that accompanies heat shock...
- Transitions between epithelial and mesenchymal states in development and diseaseBuzz Baum
Department of Cell and Developmental Biology, UCL, London, UK
Semin Cell Dev Biol 19:294-308. 2008..In this review, we discuss the common molecular and cellular mechanisms involved in EMT in both cases...
- FLIGHT: database and tools for the integration and cross-correlation of large-scale RNAi phenotypic datasetsDavid Sims
Morphogenesis Group, Ludwig Institute for Cancer Research UCL Branch, Courtauld Building, 91 Riding House Street, London, W1W 7BS, UK
Nucleic Acids Res 34:D479-83. 2006....
- Drosophila cell lines as model systems and as an experimental toolBuzz Baum
University College London Branch of the Ludwig Institute for Cancer Research, London, UK
Methods Mol Biol 420:391-424. 2008..These include techniques for growing and maintaining cell lines, transient and stable transfection, RNA interference, imaging, immunostaining, fluorescence-activated cell sorting, and for the isolation of RNA and protein from fly cells...
- Plasma microcontact patterning (PμCP): a technique for the precise control of surface patterning at small-scaleRemigio Picone
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA Department of Pediatric Oncology, Howard Hughes Medical Institute, Boston, Massachusetts, USA Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, Massachusetts, USA London Centre for Nanotechnology and Department of Medicine, University College London, London, United Kingdom Medical Research Council Laboratory for Molecular Cell Biology, University College London, London, United Kingdom CoMPLEX, University College London, London, United Kingdom
Methods Cell Biol 119:73-90. 2014..In conclusion, PμCP is a simple way to generate surface patterns that are highly reproducible, stable and uniform, making it a useful method for many applications. ..
- A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in DrosophilaJeroen Dobbelaere
The Gurdon Institute, Cambridge, United Kingdom
PLoS Biol 6:e224. 2008..Cnn is phosphorylated during mitosis in a Polo-dependent manner, suggesting that the Polo-dependent phosphorylation of Cnn initiates centrosome maturation in flies...
- A Drosophila homolog of cyclase-associated proteins collaborates with the Abl tyrosine kinase to control midline axon pathfindingZachary Wills
Department of Cell Biology and Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA
Neuron 36:611-22. 2002..These studies identify Capulet as part of an emerging pathway linking guidance signals to regulation of cytoskeletal dynamics and suggest that the Abl pathway mediates signals downstream of multiple Roundabout receptors...
- Cascade pathway of filopodia formation downstream of SCARAssel Biyasheva
Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
J Cell Sci 117:837-48. 2004..We propose a new, cascade pathway model of filopodia regulation in which SCAR signals to lamellipodia and then filopodia arise from lamellipodia in response to additional signal(s)...
- Minimizing the risk of reporting false positives in large-scale RNAi screensChristophe J Echeverri
Cenix BioScience GmbH, Tatzberg 47, Dresden, 10307, Germany
Nat Methods 3:777-9. 2006....
- Prostate-derived sterile 20-like kinase 1-alpha induces apoptosis. JNK- and caspase-dependent nuclear localization is a requirement for membrane blebbingCeniz Zihni
Kings College London, Rayne Institute, 123 Coldharbour Lane, London SE5 9NU, United Kingdom
J Biol Chem 282:6484-93. 2007....
- Actin in developmentAntonio Jacinto
Instituto Gulbenkian de Ciencia, Rua da Quinta Grande, 6, 2780 156 Oeiras, Portugal
Mech Dev 120:1337-49. 2003
- Cell shape and tissue morphogenesisJeffrey Settleman
Semin Cell Dev Biol 19:213-4. 2008
- Regulation of apicomplexan actin-based motilityJake Baum
Division of Infection and Immunity, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
Nat Rev Microbiol 4:621-8. 2006....