A Neil Barclay

Summary

Affiliation: University of Oxford
Country: UK

Publications

  1. doi request reprint The Interaction Between Signal Regulatory Protein Alpha (SIRPα) and CD47: Structure, Function, and Therapeutic Target
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK Email
    Annu Rev Immunol 32:25-50. 2014
  2. pmc Signal regulatory protein alpha (SIRPalpha)/CD47 interaction and function
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, United Kingdom
    Curr Opin Immunol 21:47-52. 2009
  3. pmc The counterbalance theory for evolution and function of paired receptors
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Immunity 29:675-8. 2008
  4. ncbi request reprint Membrane proteins with immunoglobulin-like domains--a master superfamily of interaction molecules
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Semin Immunol 15:215-23. 2003
  5. ncbi request reprint The SIRP family of receptors and immune regulation
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nat Rev Immunol 6:457-64. 2006
  6. pmc Structure of signal-regulatory protein alpha: a link to antigen receptor evolution
    Deborah Hatherley
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    J Biol Chem 284:26613-9. 2009
  7. ncbi request reprint Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family
    Gary Brooke
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    J Immunol 173:2562-70. 2004
  8. pmc Multivalent recombinant proteins for probing functions of leucocyte surface proteins such as the CD200 receptor
    Despina Voulgaraki
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Immunology 115:337-46. 2005
  9. pmc CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76
    Namir J Hassan
    Sir William Dunn School of Pathology, South Parks Rd, Oxford, OX1 3RE, United Kingdom
    Mol Cell Biol 26:6727-38. 2006
  10. ncbi request reprint Recombinant CD200 protein does not bind activating proteins closely related to CD200 receptor
    Deborah Hatherley
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    J Immunol 175:2469-74. 2005

Collaborators

Detail Information

Publications38

  1. doi request reprint The Interaction Between Signal Regulatory Protein Alpha (SIRPα) and CD47: Structure, Function, and Therapeutic Target
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK Email
    Annu Rev Immunol 32:25-50. 2014
    ....
  2. pmc Signal regulatory protein alpha (SIRPalpha)/CD47 interaction and function
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, United Kingdom
    Curr Opin Immunol 21:47-52. 2009
    ..The interaction may be modified by endocytosis of the receptors, cleavage by proteolysis and through interactions of surfactant proteins...
  3. pmc The counterbalance theory for evolution and function of paired receptors
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Immunity 29:675-8. 2008
    ....
  4. ncbi request reprint Membrane proteins with immunoglobulin-like domains--a master superfamily of interaction molecules
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Semin Immunol 15:215-23. 2003
    ..This review summarises the identification, function and properties of IgSF domains including, their size, variety of interactions, their strength of binding, role of glycosylation and organisation with other proteins...
  5. ncbi request reprint The SIRP family of receptors and immune regulation
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Nat Rev Immunol 6:457-64. 2006
    ..In this Review, we describe recent studies defining the ligands of the SIRP-family members, with particular emphasis on relating the molecular interactions of these proteins to their role in immune-cell regulation...
  6. pmc Structure of signal-regulatory protein alpha: a link to antigen receptor evolution
    Deborah Hatherley
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    J Biol Chem 284:26613-9. 2009
    ..This strengthens the suggestion from sequence analysis that SIRP is evolutionarily closely related to antigen recognition proteins...
  7. ncbi request reprint Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family
    Gary Brooke
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    J Immunol 173:2562-70. 2004
    ..The SIRPgamma-CD47 interaction may therefore not be capable of bidirectional signaling as with the SIRPalpha-CD47, but, instead, use unidirectional signaling via CD47 only...
  8. pmc Multivalent recombinant proteins for probing functions of leucocyte surface proteins such as the CD200 receptor
    Despina Voulgaraki
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Immunology 115:337-46. 2005
    ..The CD200-COMP gave strong signals in protein microarrays, suggesting that such reagents may be valuable in high throughput detection of weak interactions...
  9. pmc CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76
    Namir J Hassan
    Sir William Dunn School of Pathology, South Parks Rd, Oxford, OX1 3RE, United Kingdom
    Mol Cell Biol 26:6727-38. 2006
    ..The results have implications for regulation by CD6 and the related T-cell surface protein, CD5...
  10. ncbi request reprint Recombinant CD200 protein does not bind activating proteins closely related to CD200 receptor
    Deborah Hatherley
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    J Immunol 175:2469-74. 2005
    ..Although the ligand for the inhibitory product is a widely distributed host protein, the ligands of the activating forms remain to be identified, and one possibility is that they are pathogen components...
  11. doi request reprint Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47
    Deborah Hatherley
    Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK
    Mol Cell 31:266-77. 2008
    ..Analysis of SIRPalpha polymorphisms suggests that these, as well as the activating SIRPs, may have evolved to counteract pathogen binding to the inhibitory SIRPalpha receptor...
  12. ncbi request reprint Characterization of the CD200 receptor family in mice and humans and their interactions with CD200
    Gavin J Wright
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    J Immunol 171:3034-46. 2003
    ..Because manipulation of the CD200-CD200R interaction affects the outcome of rodent disease models, targeting of this pathway may have therapeutic utility...
  13. pmc Essential roles for Dok2 and RasGAP in CD200 receptor-mediated regulation of human myeloid cells
    Robin Mihrshahi
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    J Immunol 183:4879-86. 2009
    ....
  14. pmc Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor
    Mildred Foster-Cuevas
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    J Virol 78:7667-76. 2004
    ..We conclude that CD200 delivers local down-modulatory signals to myeloid cells through direct cell-cell contact and that the K14 viral homologue closely mimics this...
  15. pmc A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically
    S Melanie Hanna
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, United Kingdom
    BMC Biochem 4:17. 2003
    ..It is associated at the cell surface with the monocarboxylate transporters MCT1 and 4 but interactions of the extracellular region have not been characterised...
  16. pmc Identification of leucocyte surface protein interactions by high-throughput screening with multivalent reagents
    Lei Jiang
    Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
    Immunology 129:55-61. 2010
    ..The ability to detect new interactions was shown by identifying a new interaction between carcinoembryonic antigen-related cell adhesion molecule 1 and carcinoembryonic antigen-related cell adhesion molecule 8...
  17. ncbi request reprint The structure of the macrophage signal regulatory protein alpha (SIRPalpha) inhibitory receptor reveals a binding face reminiscent of that used by T cell receptors
    Deborah Hatherley
    Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
    J Biol Chem 282:14567-75. 2007
    ..The employment of loops to form the CD47-binding surface provides a mechanism for small sequence changes to modulate binding specificity, explaining the different binding properties of SIRP family members...
  18. ncbi request reprint CD200 and membrane protein interactions in the control of myeloid cells
    A Neil Barclay
    Sir William Dunn School of Pathology, University of Oxford, OX1 3RE, Oxford, UK
    Trends Immunol 23:285-90. 2002
    ..Recent data on CD200 will be reviewed in the context of other proteins that might have similar roles, in particular, the interaction between CD47 and SIRPalpha (CD172a)...
  19. pmc Dissection of agonistic and blocking effects of CD200 receptor antibodies
    Munir Akkaya
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, United Kingdom
    PLoS ONE 8:e63325. 2013
    ..The results show the importance of the repertoire of paired receptors in strains or individuals and mAb used with implications for paired receptor analysis and therapeutics...
  20. pmc Interleukin-2 signalling is modulated by a labile disulfide bond in the CD132 chain of its receptor
    Clive Metcalfe
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Open Biol 2:110036. 2012
    ....
  21. doi request reprint Immune inhibitory ligand CD200 induction by TLRs and NLRs limits macrophage activation to protect the host from meningococcal septicemia
    Subhankar Mukhopadhyay
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, UK
    Cell Host Microbe 8:236-47. 2010
    ..Thus CD200 is induced by TLR-, NOD2-, and NALP3-mediated pathways, limiting their function and protecting the host from excessive inflammation...
  22. ncbi request reprint The CD200 and CD200 receptor cell surface proteins interact through their N-terminal immunoglobulin-like domains
    Deborah Hatherley
    Sir William Dunn School of Pathology, University of Oxford, Oxford, GB
    Eur J Immunol 34:1688-94. 2004
    ..The mutagenesis showed that the binding involved the predicted GFCC' face of its N-terminal domain, like that of CD200, suggesting that the interaction evolved from a homotypic interaction...
  23. doi request reprint A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells
    Joanne E Nettleship
    The Oxford Protein Production Facility, Henry Wellcome Building for Genomic Medicine, University of Oxford, Oxford, UK
    Protein Expr Purif 62:83-9. 2008
    ..Following crystallization trials, diffracting crystals were obtained for the recombinant Fabs of OX108 and OX117, and their structures solved to 2.3A and 2.4A, respectively...
  24. pmc Labile disulfide bonds are common at the leucocyte cell surface
    Clive Metcalfe
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Open Biol 1:110010. 2011
    ..The results imply that membrane protein activity is being modulated by a 'redox regulator' mechanism...
  25. pmc Cytomegalovirus e127 protein interacts with the inhibitory CD200 receptor
    Mildred Foster-Cuevas
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    J Virol 85:6055-9. 2011
    ..It binds the host CD200R with the same affinity as that of the host protein, and thus this protein acts as a close mimic of the host protein and has the potential to downregulate immune responses to the virus...
  26. pmc New assay to detect low-affinity interactions and characterization of leukocyte receptors for collagen including leukocyte-associated Ig-like receptor-1 (LAIR-1)
    Lei Jiang
    Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
    Eur J Immunol 39:1167-75. 2009
    ..The monomeric affinities of the interactions were then determined to allow comparison with other leukocyte interactions and indicate conditions when these interactions might lead to inhibitory signals...
  27. pmc Structures of CD200/CD200 receptor family and implications for topology, regulation, and evolution
    Deborah Hatherley
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Structure 21:820-32. 2013
    ..CD200 has been acquired by herpes viruses to mimic the host interaction. CD200R has evolved rapidly presumably driven by pathogen pressure but it may also be important in homeostasis through interactions with commensal bacteria...
  28. doi request reprint Immunoregulation through membrane proteins modified by reducing conditions induced by immune reactions
    Monika Stegmann
    Oxford Molecular Pathology Institute, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, United Kingdom
    Eur J Immunol 43:15-21. 2013
    ..In this review we will concentrate on recent advances in understanding the conditions that lead to disulfide bond reduction in membrane proteins and their effects in regulating immune function...
  29. pmc Analysis of leukocyte membrane protein interactions using protein microarrays
    Michelle Letarte
    Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK
    BMC Biochem 6:2. 2005
    ..As antibody-antigen reactions are generally of higher affinity than receptor-ligand binding, we first analyzed the reactivity of monoclonal antibodies (mAb) to normal and mutant forms of immobilized CD200R...
  30. ncbi request reprint Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85
    Nicholas J Hutchings
    Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, United Kingdom
    J Biol Chem 278:22396-403. 2003
    ..These adaptor proteins are important in T cell signaling consistent with a role for CD2 in regulating pathways initiated by CMS/CIN85 and CAPZ...
  31. ncbi request reprint Frontline: Optimal T cell activation requires the engagement of CD6 and CD166
    Namir J Hassan
    Sir William Dunn School of Pathology, University of Oxford, Oxford, GB
    Eur J Immunol 34:930-40. 2004
    ..This is consistent with extracellular engagement between CD6 and CD166 being required for an optimal immune response...
  32. doi request reprint How do pathogens drive the evolution of paired receptors?
    Munir Akkaya
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    Eur J Immunol 43:303-13. 2013
    ..In this article, we review various receptor families for which pathogen interactions are known and discuss the possible molecular mechanisms driving their evolution...
  33. ncbi request reprint Phenotypic characterization of regulatory CD4+CD25+ T cells in rats
    Leigh A Stephens
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
    Int Immunol 16:365-75. 2004
    ..Our studies indicate that, at present, CD25 remains the most useful marker to enrich for regulatory CD4(+) T cells in rats and no further subdivision of the regulatory component of CD4(+)CD25(-)CD45RC(low) T cells has yet been achieved...
  34. doi request reprint Heterogeneity in the CD200R paired receptor family
    Munir Akkaya
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Immunogenetics 62:15-22. 2010
    ..It is possible that myeloid paired receptors are involved in immune regulation of responses against pathogens rather than directly killing infected cells as for NK cells and, hence, under less intense evolutionary pressure...
  35. ncbi request reprint A nomenclature for signal regulatory protein family members
    Timo K van den Berg
    Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands
    J Immunol 175:7788-9. 2005
  36. ncbi request reprint Signal regulatory proteins in the immune system
    Ellen M van Beek
    Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands
    J Immunol 175:7781-7. 2005
    ..The activating SIRPbeta proteins show considerable variability in structure and number across species and do not bind CD47. Thus the SIRP family is a rapidly evolving gene family with important roles in immune regulation...
  37. ncbi request reprint Human 6-sulfo LacNAc-expressing dendritic cells are principal producers of early interleukin-12 and are controlled by erythrocytes
    Knut Schakel
    Institute of Immunology, OncoRay, Biological and Molecular Targeting, Medical Faculty, Technische Universitat Dresden, 01307 Dresden, Germany
    Immunity 24:767-77. 2006
    ..While strictly controlled in the blood by erythrocytes, the high IL-12- and TNF-alpha-producing capacity of slanDC in tissues may be critical in fighting off pathogens; if uncontrolled, it may lead to adverse inflammatory reactions...
  38. doi request reprint A critical function for CD200 in lung immune homeostasis and the severity of influenza infection
    Robert J Snelgrove
    Imperial College London, Kennedy Institute, London, W6 8LH, UK
    Nat Immunol 9:1074-83. 2008
    ..Thus, CD200R is critical for lung macrophage immune homeostasis in the resting state and limits inflammatory amplitude and duration during pulmonary influenza infection...