Research Topics
Genomes and Genes
| A Neil BarclaySummaryAffiliation: University of Oxford Country: UK Publications
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Detail Information
Publications
Signal regulatory protein alpha (SIRPalpha)/CD47 interaction and functionA Neil Barclay
Sir William Dunn School of Pathology, University of Oxford, United Kingdom
Curr Opin Immunol 21:47-52. 2009..The interaction may be modified by endocytosis of the receptors, cleavage by proteolysis and through interactions of surfactant proteins...
The SIRP family of receptors and immune regulationA Neil Barclay
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Nat Rev Immunol 6:457-64. 2006..In this Review, we describe recent studies defining the ligands of the SIRP-family members, with particular emphasis on relating the molecular interactions of these proteins to their role in immune-cell regulation...- The counterbalance theory for evolution and function of paired receptorsA Neil Barclay
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
Immunity 29:675-8. 2008.... - Membrane proteins with immunoglobulin-like domains--a master superfamily of interaction moleculesA Neil Barclay
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Semin Immunol 15:215-23. 2003..This review summarises the identification, function and properties of IgSF domains including, their size, variety of interactions, their strength of binding, role of glycosylation and organisation with other proteins... - Structure of signal-regulatory protein alpha: a link to antigen receptor evolutionDeborah Hatherley
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
J Biol Chem 284:26613-9. 2009..This strengthens the suggestion from sequence analysis that SIRP is evolutionarily closely related to antigen recognition proteins... - Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) familyGary Brooke
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
J Immunol 173:2562-70. 2004..The SIRPgamma-CD47 interaction may therefore not be capable of bidirectional signaling as with the SIRPalpha-CD47, but, instead, use unidirectional signaling via CD47 only... - Multivalent recombinant proteins for probing functions of leucocyte surface proteins such as the CD200 receptorDespina Voulgaraki
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Immunology 115:337-46. 2005..The CD200-COMP gave strong signals in protein microarrays, suggesting that such reagents may be valuable in high throughput detection of weak interactions... - Recombinant CD200 protein does not bind activating proteins closely related to CD200 receptorDeborah Hatherley
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
J Immunol 175:2469-74. 2005..Although the ligand for the inhibitory product is a widely distributed host protein, the ligands of the activating forms remain to be identified, and one possibility is that they are pathogen components... - CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76Namir J Hassan
Sir William Dunn School of Pathology, South Parks Rd, Oxford, OX1 3RE, United Kingdom
Mol Cell Biol 26:6727-38. 2006..The results have implications for regulation by CD6 and the related T-cell surface protein, CD5... - Characterization of the CD200 receptor family in mice and humans and their interactions with CD200Gavin J Wright
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
J Immunol 171:3034-46. 2003..Because manipulation of the CD200-CD200R interaction affects the outcome of rodent disease models, targeting of this pathway may have therapeutic utility... 
Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47Deborah Hatherley
Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK
Mol Cell 31:266-77. 2008..Analysis of SIRPalpha polymorphisms suggests that these, as well as the activating SIRPs, may have evolved to counteract pathogen binding to the inhibitory SIRPalpha receptor...- Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptorMildred Foster-Cuevas
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
J Virol 78:7667-76. 2004..We conclude that CD200 delivers local down-modulatory signals to myeloid cells through direct cell-cell contact and that the K14 viral homologue closely mimics this... - Essential roles for Dok2 and RasGAP in CD200 receptor-mediated regulation of human myeloid cellsRobin Mihrshahi
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
J Immunol 183:4879-86. 2009.... - A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilicallyS Melanie Hanna
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, United Kingdom
BMC Biochem 4:17. 2003..It is associated at the cell surface with the monocarboxylate transporters MCT1 and 4 but interactions of the extracellular region have not been characterised... - Identification of leucocyte surface protein interactions by high-throughput screening with multivalent reagentsLei Jiang
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
Immunology 129:55-61. 2010..The ability to detect new interactions was shown by identifying a new interaction between carcinoembryonic antigen-related cell adhesion molecule 1 and carcinoembryonic antigen-related cell adhesion molecule 8... - The structure of the macrophage signal regulatory protein alpha (SIRPalpha) inhibitory receptor reveals a binding face reminiscent of that used by T cell receptorsDeborah Hatherley
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
J Biol Chem 282:14567-75. 2007..The employment of loops to form the CD47-binding surface provides a mechanism for small sequence changes to modulate binding specificity, explaining the different binding properties of SIRP family members... - CD200 and membrane protein interactions in the control of myeloid cellsA Neil Barclay
Sir William Dunn School of Pathology, University of Oxford, OX1 3RE, Oxford, UK
Trends Immunol 23:285-90. 2002..Recent data on CD200 will be reviewed in the context of other proteins that might have similar roles, in particular, the interaction between CD47 and SIRPalpha (CD172a)... - A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cellsJoanne E Nettleship
The Oxford Protein Production Facility, Henry Wellcome Building for Genomic Medicine, University of Oxford, Oxford, UK
Protein Expr Purif 62:83-9. 2008..Following crystallization trials, diffracting crystals were obtained for the recombinant Fabs of OX108 and OX117, and their structures solved to 2.3A and 2.4A, respectively... - Immune inhibitory ligand CD200 induction by TLRs and NLRs limits macrophage activation to protect the host from meningococcal septicemiaSubhankar Mukhopadhyay
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, UK
Cell Host Microbe 8:236-47. 2010..Thus CD200 is induced by TLR-, NOD2-, and NALP3-mediated pathways, limiting their function and protecting the host from excessive inflammation... - The CD200 and CD200 receptor cell surface proteins interact through their N-terminal immunoglobulin-like domainsDeborah Hatherley
Sir William Dunn School of Pathology, University of Oxford, Oxford, GB
Eur J Immunol 34:1688-94. 2004..The mutagenesis showed that the binding involved the predicted GFCC' face of its N-terminal domain, like that of CD200, suggesting that the interaction evolved from a homotypic interaction... - New assay to detect low-affinity interactions and characterization of leukocyte receptors for collagen including leukocyte-associated Ig-like receptor-1 (LAIR-1)Lei Jiang
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
Eur J Immunol 39:1167-75. 2009..The monomeric affinities of the interactions were then determined to allow comparison with other leukocyte interactions and indicate conditions when these interactions might lead to inhibitory signals... - Cytomegalovirus e127 protein interacts with the inhibitory CD200 receptorMildred Foster-Cuevas
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
J Virol 85:6055-9. 2011..It binds the host CD200R with the same affinity as that of the host protein, and thus this protein acts as a close mimic of the host protein and has the potential to downregulate immune responses to the virus... - Analysis of leukocyte membrane protein interactions using protein microarraysMichelle Letarte
Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK
BMC Biochem 6:2. 2005..As antibody-antigen reactions are generally of higher affinity than receptor-ligand binding, we first analyzed the reactivity of monoclonal antibodies (mAb) to normal and mutant forms of immobilized CD200R... - Immunoregulation through membrane proteins modified by reducing conditions induced by immune reactionsMonika Stegmann
Oxford Molecular Pathology Institute, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, United Kingdom
Eur J Immunol 43:15-21. 2013..In this review we will concentrate on recent advances in understanding the conditions that lead to disulfide bond reduction in membrane proteins and their effects in regulating immune function... - Frontline: Optimal T cell activation requires the engagement of CD6 and CD166Namir J Hassan
Sir William Dunn School of Pathology, University of Oxford, Oxford, GB
Eur J Immunol 34:930-40. 2004..This is consistent with extracellular engagement between CD6 and CD166 being required for an optimal immune response... - Linking the T cell surface protein CD2 to the actin-capping protein CAPZ via CMS and CIN85Nicholas J Hutchings
Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, United Kingdom
J Biol Chem 278:22396-403. 2003..These adaptor proteins are important in T cell signaling consistent with a role for CD2 in regulating pathways initiated by CMS/CIN85 and CAPZ... - Phenotypic characterization of regulatory CD4+CD25+ T cells in ratsLeigh A Stephens
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK
Int Immunol 16:365-75. 2004..Our studies indicate that, at present, CD25 remains the most useful marker to enrich for regulatory CD4(+) T cells in rats and no further subdivision of the regulatory component of CD4(+)CD25(-)CD45RC(low) T cells has yet been achieved... - Heterogeneity in the CD200R paired receptor familyMunir Akkaya
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Immunogenetics 62:15-22. 2010..It is possible that myeloid paired receptors are involved in immune regulation of responses against pathogens rather than directly killing infected cells as for NK cells and, hence, under less intense evolutionary pressure... - How do pathogens drive the evolution of paired receptors?Munir Akkaya
Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
Eur J Immunol 43:303-13. 2013..In this article, we review various receptor families for which pathogen interactions are known and discuss the possible molecular mechanisms driving their evolution... - A nomenclature for signal regulatory protein family membersTimo K van den Berg
Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands
J Immunol 175:7788-9. 2005 - Signal regulatory proteins in the immune systemEllen M van Beek
Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands
J Immunol 175:7781-7. 2005..The activating SIRPbeta proteins show considerable variability in structure and number across species and do not bind CD47. Thus the SIRP family is a rapidly evolving gene family with important roles in immune regulation... - Human 6-sulfo LacNAc-expressing dendritic cells are principal producers of early interleukin-12 and are controlled by erythrocytesKnut Schakel
Institute of Immunology, OncoRay, Biological and Molecular Targeting, Medical Faculty, Technische Universitat Dresden, 01307 Dresden, Germany
Immunity 24:767-77. 2006..While strictly controlled in the blood by erythrocytes, the high IL-12- and TNF-alpha-producing capacity of slanDC in tissues may be critical in fighting off pathogens; if uncontrolled, it may lead to adverse inflammatory reactions... - A critical function for CD200 in lung immune homeostasis and the severity of influenza infectionRobert J Snelgrove
Imperial College London, Kennedy Institute, London, W6 8LH, UK
Nat Immunol 9:1074-83. 2008..Thus, CD200R is critical for lung macrophage immune homeostasis in the resting state and limits inflammatory amplitude and duration during pulmonary influenza infection...