Lyle Armstrong

Summary

Affiliation: University of Newcastle
Country: UK

Publications

  1. doi request reprint Epigenetic control of embryonic stem cell differentiation
    Lyle Armstrong
    Institute of Genetic Medicine, Newcastle University, The International Centre For Life, Central Parkway, Newcastle upon Tyne, UK
    Stem Cell Rev 8:67-77. 2012
  2. ncbi request reprint The future of human nuclear transfer?
    Lyle Armstrong
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, International Centre for Life, Central Parkway, UK
    Stem Cell Rev 2:351-8. 2006
  3. doi request reprint Editorial: Our Top 10 Developments in Stem Cell Biology over the Last 30 Years
    Lyle Armstrong
    The Institute of Genetic Medicine, Newcastle University, The International Centre For Life, Central Parkway, Newcastle upon Tyne, United Kingdom
    Stem Cells 30:2-9. 2012
  4. ncbi request reprint Epigenetic modification is central to genome reprogramming in somatic cell nuclear transfer
    Lyle Armstrong
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, International Centre for Life, Newcastle upon Tyne NE1 3BZ, UK
    Stem Cells 24:805-14. 2006
  5. doi request reprint Human induced pluripotent stem cell lines show stress defense mechanisms and mitochondrial regulation similar to those of human embryonic stem cells
    Lyle Armstrong
    Institute of Human Genetics, Newcastle University, International Centre for Life, Newcastle upon Tyne, United Kingdom
    Stem Cells 28:661-73. 2010
  6. doi request reprint Expression of GFP under the control of the RNA helicase VASA permits fluorescence-activated cell sorting isolation of human primordial germ cells
    Katarzyna Tilgner
    North East Stem Cell Institute, Newcastle University, International Centre for Life, NE1 3BZ, United Kingdom
    Stem Cells 28:84-92. 2010
  7. doi request reprint An important role for CDK2 in G1 to S checkpoint activation and DNA damage response in human embryonic stem cells
    Irina Neganova
    Institute of Human Genetics, Newcastle University, International Centre for Life, Newcastle upon Tyne, United Kingdom
    Stem Cells 29:651-9. 2011
  8. ncbi request reprint Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages
    Louise Hyslop
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle International Centre for Life, Newcastle NE1 3BZ, United Kingdom
    Stem Cells 23:1035-43. 2005
  9. ncbi request reprint An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells
    Petra Stojkovic
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK
    Stem Cells 23:306-14. 2005
  10. doi request reprint Isolation of primordial germ cells from differentiating human embryonic stem cells
    Katarzyna Tilgner
    North East Institute for Stem Cell Research, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
    Stem Cells 26:3075-85. 2008

Collaborators

Detail Information

Publications38

  1. doi request reprint Epigenetic control of embryonic stem cell differentiation
    Lyle Armstrong
    Institute of Genetic Medicine, Newcastle University, The International Centre For Life, Central Parkway, Newcastle upon Tyne, UK
    Stem Cell Rev 8:67-77. 2012
    ..By deriving embryonic stem cells from the early embryo, we can preserve the highly specialised genome organisation and this has permitted several detailed investigations into the molecular basis of pluripotency...
  2. ncbi request reprint The future of human nuclear transfer?
    Lyle Armstrong
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, International Centre for Life, Central Parkway, UK
    Stem Cell Rev 2:351-8. 2006
  3. doi request reprint Editorial: Our Top 10 Developments in Stem Cell Biology over the Last 30 Years
    Lyle Armstrong
    The Institute of Genetic Medicine, Newcastle University, The International Centre For Life, Central Parkway, Newcastle upon Tyne, United Kingdom
    Stem Cells 30:2-9. 2012
    ..stem cells 2012;30:2-9...
  4. ncbi request reprint Epigenetic modification is central to genome reprogramming in somatic cell nuclear transfer
    Lyle Armstrong
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, International Centre for Life, Newcastle upon Tyne NE1 3BZ, UK
    Stem Cells 24:805-14. 2006
    ..This review focuses on the mechanisms by which this is achieved and how this may account for its partial failure in the "cloning" process. We also highlight the potential dangers this may introduce into ESCs produced by this technology...
  5. doi request reprint Human induced pluripotent stem cell lines show stress defense mechanisms and mitochondrial regulation similar to those of human embryonic stem cells
    Lyle Armstrong
    Institute of Human Genetics, Newcastle University, International Centre for Life, Newcastle upon Tyne, United Kingdom
    Stem Cells 28:661-73. 2010
    ..Together our data suggest that, during the reprogramming process, certain cellular mechanisms are in place to ensure that hiPSC are provided with the same defense mechanisms against accumulation of ROS as the hESC...
  6. doi request reprint Expression of GFP under the control of the RNA helicase VASA permits fluorescence-activated cell sorting isolation of human primordial germ cells
    Katarzyna Tilgner
    North East Stem Cell Institute, Newcastle University, International Centre for Life, NE1 3BZ, United Kingdom
    Stem Cells 28:84-92. 2010
    ..This population shows a different pattern of chromatin modifications to those obtained by FACS enrichment of Stage Specific Antigen one expressing cells in our previous publication...
  7. doi request reprint An important role for CDK2 in G1 to S checkpoint activation and DNA damage response in human embryonic stem cells
    Irina Neganova
    Institute of Human Genetics, Newcastle University, International Centre for Life, Newcastle upon Tyne, United Kingdom
    Stem Cells 29:651-9. 2011
    ....
  8. ncbi request reprint Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages
    Louise Hyslop
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle International Centre for Life, Newcastle NE1 3BZ, United Kingdom
    Stem Cells 23:1035-43. 2005
    ..Taken together, our findings suggest that NANOG acts as a gatekeeper of pluripotency in human embryonic stem and carcinoma cells by preventing their differentiation to extraembryonic endoderm and trophectoderm lineages...
  9. ncbi request reprint An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells
    Petra Stojkovic
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK
    Stem Cells 23:306-14. 2005
    ..Feeder cells derived from hESCs offers a potentially more secure autogeneic and genotypically homogenous system for the growth of undifferentiated hESCs...
  10. doi request reprint Isolation of primordial germ cells from differentiating human embryonic stem cells
    Katarzyna Tilgner
    North East Institute for Stem Cell Research, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom
    Stem Cells 26:3075-85. 2008
    ..These primordial germ cells demonstrate high-level expression of the germ cell-specific VASA gene and show removal of parental imprints and chromatin modification changes that support their primordial germ cell identity...
  11. ncbi request reprint Human-serum matrix supports undifferentiated growth of human embryonic stem cells
    Petra Stojkovic
    Centre for Stem Cell Biology and Developmental Genetics, Institute of Human Genetics, University of Newcastle, Newcastle upon Tyne, UK
    Stem Cells 23:895-902. 2005
    ....
  12. pmc A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A
    Xin Zhang
    NorthEast England Stem Cell Institute, Institute of Human Genetics, Newcastle University, International Centre for Life, Newcastle upon Tyne, England, UK
    J Cell Biol 184:67-82. 2009
    ..Overexpression of CDK6 or CDC25A alone can rescue the impact of NANOG down-regulation on S-phase entry, suggesting that CDK6 and CDC25A are downstream cell cycle effectors of NANOG during the G1 to S transition...
  13. doi request reprint Epigenetic landscaping during hESC differentiation to neural cells
    Anna Golebiewska
    Institute of Human Genetics, University of Newcastle upon Tyne, International Centre for Life, United Kingdom
    Stem Cells 27:1298-308. 2009
    ..However, they differ significantly from the bivalent domains observed in hESCs, with a possible role of dimethylation of lysine 9 H3 in repressing the poised genes...
  14. ncbi request reprint Differentiation of human embryonic stem cells into corneal epithelial-like cells by in vitro replication of the corneal epithelial stem cell niche
    Sajjad Ahmad
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, Newcastle upon Tyne, United Kingdom
    Stem Cells 25:1145-55. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  15. doi request reprint Efficient hematopoietic differentiation of human embryonic stem cells on stromal cells derived from hematopoietic niches
    Maria H Ledran
    North East Institute for Stem Cell Research, Newcastle University, Newcastle upon Tyne, UK
    Cell Stem Cell 3:85-98. 2008
    ..Overall, our findings represent significant progress toward the goal of deriving functional hematopoietic stem cells from hESCs...
  16. doi request reprint A putative role for RHAMM/HMMR as a negative marker of stem cell-containing population of human limbal epithelial cells
    Sajjad Ahmad
    North East Stem Cell Institute and, Institute of Human Genetics, University of Newcastle upon Tyne, International Centre for Life, Newcastle upon Tyne, United Kingdom
    Stem Cells 26:1609-19. 2008
    ..Combination of these two cell selection methods presents a novel method for LSC enrichment and characterization. Disclosure of potential conflicts of interest is found at the end of this article...
  17. doi request reprint Opposing putative roles for canonical and noncanonical NFκB signaling on the survival, proliferation, and differentiation potential of human embryonic stem cells
    Chunbo Yang
    Institute of Human Genetics, International Centre for Life, Newcastle University, Newcastle upon Tyne, United Kingdom
    Stem Cells 28:1970-80. 2010
    ....
  18. ncbi request reprint The role of PI3K/AKT, MAPK/ERK and NFkappabeta signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis
    Lyle Armstrong
    Centre for Stem Cell Biology and Developmental Genetics, Institute of Human Genetics, University of Newcastle, Central Parkways, Newcastle upon Tyne, UK
    Hum Mol Genet 15:1894-913. 2006
    ..Disruption of these pathways in isolation using specific inhibitors resulted in loss of pluripotency and/or loss of viability suggesting the importance of such signalling pathways in embryonic stem cell maintenance...
  19. pmc Derivation and functional analysis of patient-specific induced pluripotent stem cells as an in vitro model of chronic granulomatous disease
    Yan Jiang
    Institute of Genetic Medicine, Newcastle University, Newcastle, UK
    Stem Cells 30:599-611. 2012
    ..Together these results suggest that CGD-patient-specific iPSC lines represent an important tool for modeling CGD disease phenotypes, screening candidate drugs, and the development of gene therapy...
  20. doi request reprint Large-scale transcriptional profiling and functional assays reveal important roles for Rho-GTPase signalling and SCL during haematopoietic differentiation of human embryonic stem cells
    Sun Yung
    Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, UK
    Hum Mol Genet 20:4932-46. 2011
    ..Importantly, intrasplenic transplantation of SCL-overexpressing hESC-derived haematopoietic cells enhanced recovery from induced acute anaemia without significant cell engraftment, suggesting a paracrine-mediated effect...
  21. doi request reprint A key role for telomerase reverse transcriptase unit in modulating human embryonic stem cell proliferation, cell cycle dynamics, and in vitro differentiation
    Chunbo Yang
    North East Institute for Stem Cell Research, Newcastle upon Tyne NE1 3BZ, United Kingdom
    Stem Cells 26:850-63. 2008
    ..Our results indicate for the first time an important role for TERT in the maintenance of human ESC pluripotency, cell cycle regulation, and in vitro differentiation capacity...
  22. doi request reprint Epigenetic marking prepares the human HOXA cluster for activation during differentiation of pluripotent cells
    Stuart P Atkinson
    North East Institute for Stem Cell Research, University of Newcastle upon Tyne, International Centre for Life, Newcastle upon Tyne, United Kingdom
    Stem Cells 26:1174-85. 2008
    ..The precise locations of such modified histones may be involved in controlling the colinear expression of genes from the cluster...
  23. ncbi request reprint Downregulation of multiple stress defense mechanisms during differentiation of human embryonic stem cells
    Gabriele Saretzki
    Crucible Lab, Institute of Human Genetics, Central Parkway, Newcastle upon Tyne NE1 3BZ, United Kingdom
    Stem Cells 26:455-64. 2008
    ..These results confirm earlier data obtained during mouse embryonic stem cell differentiation and are in accordance with evolutionary predictions...
  24. ncbi request reprint Derivation of a human blastocyst after heterologous nuclear transfer to donated oocytes
    Miodrag Stojkovic
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, UK
    Reprod Biomed Online 11:226-31. 2005
    ..However, if the promise of this new science is to achieve its potential in the foreseeable future, it will be necessary to identify new sources of oocytes that can be used immediately after retrieval...
  25. doi request reprint A putative role for the immunoproteasome in the maintenance of pluripotency in human embryonic stem cells
    Stuart P Atkinson
    Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom
    Stem Cells 30:1373-84. 2012
    ....
  26. doi request reprint Brief report: a human induced pluripotent stem cell model of cernunnos deficiency reveals an important role for XLF in the survival of the primitive hematopoietic progenitors
    Katarzyna Tilgner
    Institute of Genetic Medicine, Newcastle University, Newcastle, United Kingdom NESCI, Newcastle University, Newcastle, United Kingdom
    Stem Cells 31:2015-23. 2013
    ..Together, our findings highlight the importance of NHEJ-mediated-DNA repair in the maintenance of a pristine pool of hematopoietic progenitors during human embryonic development...
  27. doi request reprint Brief report: human pluripotent stem cell models of fanconi anemia deficiency reveal an important role for fanconi anemia proteins in cellular reprogramming and survival of hematopoietic progenitors
    Sun K Yung
    Institute of Genetic Medicine, Newcastle University, Newcastle, United Kingdom
    Stem Cells 31:1022-9. 2013
    ..Together these data highlight the critical requirement for FA proteins in survival of hematopoietic progenitors, cellular reprogramming, and maintenance of genomic stability...
  28. ncbi request reprint Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture
    Miodrag Stojkovic
    Institute of Human Genetics, University of Newcastle, Central Parkway, Newcastle upon Tyne, NE1 3BZ, UK
    Stem Cells 22:790-7. 2004
    ..The pluripotency of the cell line was confirmed by the formation of teratomas after injection into severely combined immunodeficient mice and spontaneous differentiation under in vitro conditions...
  29. ncbi request reprint Phenotypic characterization of murine primitive hematopoietic progenitor cells isolated on basis of aldehyde dehydrogenase activity
    Lyle Armstrong
    Institute of Human Genetics, University of Newcastle upon Tyne, International Centre for Life, Newcastle upon Tyne, UK
    Stem Cells 22:1142-51. 2004
    ..These data strongly suggest that progenitor enrichment from Lin- cells on the basis of ALDH is a valid method whose simplicity of application makes it advantageous over conventional separations...
  30. ncbi request reprint Derivation of human embryonic stem cells from developing and arrested embryos
    Xin Zhang
    Centre for Stem Cell Biology and Developmental Genetics, University of Newcastle, Newcastle upon Tyne, United Kingdom
    Stem Cells 24:2669-76. 2006
    ..These results demonstrate that arrested embryos are additional valuable resources to surplus and donated developing embryos and should be used to study early human development or derive pluripotent hESC...
  31. ncbi request reprint Epigenetics in embryonic stem cells: regulation of pluripotency and differentiation
    Stuart Atkinson
    Institute of Human Genetics, Newcastle University, Newcastle, UK
    Cell Tissue Res 331:23-9. 2008
    ..Additionally, epigenetic mechanisms have been indicated to play a role in the formation of the pluripotent cells of the inner cell mass in the developing blastocyst...
  32. ncbi request reprint Human embryonic stem cells: biology and clinical implications
    Louise A Hyslop
    Centre for Stem Cell Biology and Developmental Genetics, Institute of Human Genetics, ICFL, Central Parkway, Newcastle, NE1 3BZ, UK
    Expert Rev Mol Med 7:1-21. 2005
    ....
  33. pmc Potential for pharmacological manipulation of human embryonic stem cells
    Stuart P Atkinson
    Centro de Investigacion Principe Felipe, Valencia, Spain
    Br J Pharmacol 169:269-89. 2013
    ..Within this review, we hope to describe the current uses of small molecule inhibitors/activators in hESC biology and their potential uses in the future...
  34. pmc A novel model of urinary tract differentiation, tissue regeneration, and disease: reprogramming human prostate and bladder cells into induced pluripotent stem cells
    Mohammad Moad
    Northern Institute for Cancer Research, Newcastle University, UK
    Eur Urol 64:753-61. 2013
    ..These cells, termed induced pluripotent stem cells (iPSCs), could theoretically generate adult prostate and bladder tissue, providing an alternative strategy to study differentiation...
  35. ncbi request reprint Stress defense in murine embryonic stem cells is superior to that of various differentiated murine cells
    Gabriele Saretzki
    Henry Wellcome Laboratory for Biogerontology, Newcastle General Hospital, University of Newcastle upon Tyne, Newcastle upon Tyne NE4 6BE, UK
    Stem Cells 22:962-71. 2004
    ....
  36. ncbi request reprint A role for nucleoprotein Zap3 in the reduction of telomerase activity during embryonic stem cell differentiation
    Lyle Armstrong
    School of Biological and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, UK
    Mech Dev 121:1509-22. 2004
    ....
  37. doi request reprint Law should recognize value of interspecies embryos
    Justin C St John
    Nature 451:627. 2008
  38. doi request reprint Silencing of the expression of pluripotent driven-reporter genes stably transfected into human pluripotent cells
    Rebecca Stewart
    North East Institute for Stem Cell Research, Newcastle University International Centre for Life, Newcastle NE13BZ, UK
    Regen Med 3:505-22. 2008
    ..Our aim was to use core promoter regions from such pluripotent genes to drive expression of reporter genes that would be suitable for human ES cell selection amongst differentiated cells...