Genomes and Genes
Affiliation: University of Cambridge
- Polo-like kinases: conservation and divergence in their functions and regulationVincent Archambault
Cancer Research UK, Cell Cycle Genetics Research Group, University of Cambridge, Department of Genetics, Downing Street, Cambridge, CB2 3EH, UK
Nat Rev Mol Cell Biol 10:265-75. 2009..Plks are now recognized to link cell division to developmental processes and to function in differentiated cells. A comparison of Plk function and regulation between organisms offers insight into the rich variations of cell division...
- A bitter PP1 fights the sweet poloVincent Archambault
Department of Genetics, Cancer Research UK Cell Cycle Genetics Research Group, University of Cambridge, Downing Street, CB2 3EH Cambridge, UK
Mol Cell 30:541-2. 2008..2008) report that the PP1 regulatory subunit MYPT1 interacts with PLK1 and antagonizes essential mitotic functions of PLK1, at least in part by promoting the dephosphorylation of PLK1 at Thr210...
- Yeast Polo-like kinase substrates are nailed with the right toolsVincent Archambault
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
Genome Biol 9:203. 2008..A novel role for this kinase in regulating the mitotic spindle is revealed...
- Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis in Drosophila spermatocytesEmilie Montembault
Department of Pathology, University of Cambridge, Cambridge CB2 1QP, England, UK
J Cell Biol 191:1351-65. 2010..Our findings indicate that Nesd is a novel carbohydrate-binding protein that functions together with centralspindlin in late cytokinesis, thus highlighting the importance of glycosylation in this process...
- Recruitment of Polo kinase to the spindle midzone during cytokinesis requires the Feo/Klp3A complexPier Paolo D'Avino
Cancer Research UK Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Cambridge, United Kingdom
PLoS ONE 2:e572. 2007..Their roles during cytokinesis, however, are not well understood because the requirement of these kinases during early stages of mitosis complicates the study of their functions after anaphase onset...
- Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo developmentSarah P Blagden
Cancer Research UK Cell Cycle Genetics Group, University of Cambridge, Department of Genetics, Cambridge CB2 3EH, UK
Dev Biol 334:186-97. 2009..We discuss why the syncytial mitotic cycles and male meiosis should have a particularly sensitive requirement for Larp proteins in regulating not only transcript stability but also potentially the translation of mRNAs...
- Rab5 GTPase controls chromosome alignment through Lamin disassembly and relocation of the NuMA-like protein Mud to the poles during mitosisLuisa Capalbo
Cancer Research United Kingdom Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom
Proc Natl Acad Sci U S A 108:17343-8. 2011..Our results indicate a role for Rab5 in mitosis and reinforce the emerging view of the contributions made by cell membrane dynamics to spindle function...
- Molecular analysis of core kinetochore composition and assembly in Drosophila melanogasterMarcin R Przewloka
Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Cambridge, United Kingdom
PLoS ONE 2:e478. 2007..Although Drosophila is a classical model organism for studies of chromosome segregation, little is known about the organization of its kinetochores...
- Sequestration of Polo kinase to microtubules by phosphopriming-independent binding to Map205 is relieved by phosphorylation at a CDK site in mitosisVincent Archambault
Department of Genetics, University of Cambridge, Cambridge, CB2 3EH, United Kingdom
Genes Dev 22:2707-20. 2008..We propose that Map205-dependent targeting of Polo to microtubules provides a stable reservoir of Polo that can be rapidly mobilized by the activity of Cdk1 at mitotic entry...
- Isolation of protein complexes involved in mitosis and cytokinesis from Drosophila cultured cellsPier Paolo D'Avino
Cancer Research UK Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Cambridge, UK
Methods Mol Biol 545:99-112. 2009..Although this method has proven very successful in isolating mitotic and cytokinetic complexes, it can also be used to characterise protein complexes involved in many other cellular processes...
- Mutations in Drosophila Greatwall/Scant reveal its roles in mitosis and meiosis and interdependence with Polo kinaseVincent Archambault
CRUK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge, United Kingdom
PLoS Genet 3:e200. 2007..Our results indicate that Gwl activity antagonizes Polo and thus identify an important regulatory interaction of the cell cycle...
- Multiple protein phosphatases are required for mitosis in DrosophilaFeng Chen
Cancer Research United Kingdom, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, United Kingdom
Curr Biol 17:293-303. 2007..Approximately one-third of the Drosophila kinome has been ascribed some cell-cycle function. However, little is known about which of its 117 protein phosphatases (PPs) or subunits have counteracting roles...
- Cell cycle: proteomics gives it a spinVincent Archambault
Department of Genetics, University of Cambridge, Downing Street, CB2 3EH, UK
Expert Rev Proteomics 2:615-25. 2005..The near future should see the application of more quantitative proteomic approaches to probe the dynamic aspects of the molecular system that underlie the cell cycle in model organisms and in human cells...
- Cyclin and cyclin-dependent kinase substrate requirements for preventing rereplication reveal the need for concomitant activation and inhibitionAmy E Ikui
The Rockefeller University, New York, New York 10021, USA
Genetics 175:1011-22. 2007....
- Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exitVincent Archambault
The Rockefeller University, New York, New York 10021, USA
Mol Biol Cell 14:4592-604. 2003..We conclude, therefore, that although both Cdc6 and Sic1 have the potential to facilitate mitotic exit by inhibiting Clb2-Cdk, mitotic exit nevertheless does not require any identified stoichiometric inhibitor of Cdk activity...
- Targeted proteomic study of the cyclin-Cdk moduleVincent Archambault
The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Mol Cell 14:699-711. 2004..Our results demonstrate that this approach can be used to detect a host of transient and dynamic protein associations within a biological module...
- Testing a mathematical model of the yeast cell cycleFrederick R Cross
The Rockefeller University, New York, NY 10021, USA
Mol Biol Cell 13:52-70. 2002..Thus, the model is a strong but incomplete attempt at a realistic representation of cell cycle control. Constraints of the sort developed here will be important in development of a truly predictive model...
- Disruption of mechanisms that prevent rereplication triggers a DNA damage responseVincent Archambault
The Rockefeller University, 1230 York Ave, Box 237, New York, NY 10021, USA
Mol Cell Biol 25:6707-21. 2005..Our results implicate an Mre11-Mec1-dependent pathway in limiting the extent of rereplication...
- Two-faced cyclins with eyes on the targetsVincent Archambault
Rockefeller University, New York, New York, USA
Cell Cycle 4:125-30. 2005..The evolutionary conservation of the HP motif suggests that it allows cyclins to carry out important and specialized functions...
- Interaction of the S-phase cyclin Clb5 with an "RXL" docking sequence in the initiator protein Orc6 provides an origin-localized replication control switchGwendolyn M Wilmes
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Genes Dev 18:981-91. 2004..We propose that Clb5 binding to ORC provides an origin-localized replication control switch that specifically prevents reinitiation at replicated origins...
- Analysis of protein phosphorylation by hypothesis-driven multiple-stage mass spectrometryEmmanuel J Chang
Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
Anal Chem 76:4472-83. 2004..Our results demonstrate that HMS-MS is a sensitive, highly specific tool for systematically surveying proteins for Ser/Thr phosphorylation, and represents a significant step toward our goal of comprehensive phosphorylation mapping...