Peter W Andrews

Summary

Affiliation: University of Sheffield
Country: UK

Publications

  1. pmc From teratocarcinomas to embryonic stem cells
    Peter W Andrews
    Department of Biomedical Science, Western Bank, University of Sheffield, Sheffield S10 2TN, UK
    Philos Trans R Soc Lond B Biol Sci 357:405-17. 2002
  2. pmc Surface antigens of human embryonic stem cells: changes upon differentiation in culture
    Jonathan S Draper
    Department of Biomedical Science, University of Sheffield, UK
    J Anat 200:249-58. 2002
  3. ncbi request reprint Expression of Wnt and Notch pathway genes in a pluripotent human embryonal carcinoma cell line and embryonic stem cell
    James Walsh
    Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    APMIS 111:197-210; discussion 210-1. 2003
  4. pmc Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors
    Jamie M Sperger
    Wisconsin National Primate Research Center and Department of Anatomy, School of Medicine, University of Wisconsin, Madison, WI 53715, USA
    Proc Natl Acad Sci U S A 100:13350-5. 2003
  5. ncbi request reprint Culture and characterization of human embryonic stem cells
    Jonathan S Draper
    Centre for Stem Cell Biology and Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Stem Cells Dev 13:325-36. 2004
  6. pmc Molecular mechanisms of pluripotency and reprogramming
    Jie Na
    School of Medicine, Tsinghua University, Beijing, 100084 China
    Stem Cell Res Ther 1:33. 2010
  7. pmc Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells
    Veronika Akopian
    The Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    In Vitro Cell Dev Biol Anim 46:247-58. 2010
  8. ncbi request reprint Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: opposite sides of the same coin
    P W Andrews
    The Centre for Stem Cell Biology and Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Biochem Soc Trans 33:1526-30. 2005
  9. ncbi request reprint The International Stem Cell Initiative: toward benchmarks for human embryonic stem cell research
    Peter W Andrews
    Department of Biomedical Sciences, University of Sheffield, Sheffield S10 2TN, UK
    Nat Biotechnol 23:795-7. 2005
  10. doi request reprint Pinacidil enhances survival of cryopreserved human embryonic stem cells
    Ivana Barbaric
    Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield, UK
    Cryobiology 63:298-305. 2011

Detail Information

Publications54

  1. pmc From teratocarcinomas to embryonic stem cells
    Peter W Andrews
    Department of Biomedical Science, Western Bank, University of Sheffield, Sheffield S10 2TN, UK
    Philos Trans R Soc Lond B Biol Sci 357:405-17. 2002
    ....
  2. pmc Surface antigens of human embryonic stem cells: changes upon differentiation in culture
    Jonathan S Draper
    Department of Biomedical Science, University of Sheffield, UK
    J Anat 200:249-58. 2002
    ..In all of these features the human ES cells, and their pattern of differentiation, resembled the pluripotent human EC cell line NTERA-2 although clearly the range of cells generated by the hES cells was considerably greater...
  3. ncbi request reprint Expression of Wnt and Notch pathway genes in a pluripotent human embryonal carcinoma cell line and embryonic stem cell
    James Walsh
    Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    APMIS 111:197-210; discussion 210-1. 2003
    ..We present a model in which interactions between and regulation of Wnt and Notch signalling are important in maintaining EC/ES stem cells and also controlling their differentiation...
  4. pmc Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors
    Jamie M Sperger
    Wisconsin National Primate Research Center and Department of Anatomy, School of Medicine, University of Wisconsin, Madison, WI 53715, USA
    Proc Natl Acad Sci U S A 100:13350-5. 2003
    ..These genes are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype...
  5. ncbi request reprint Culture and characterization of human embryonic stem cells
    Jonathan S Draper
    Centre for Stem Cell Biology and Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Stem Cells Dev 13:325-36. 2004
    ....
  6. pmc Molecular mechanisms of pluripotency and reprogramming
    Jie Na
    School of Medicine, Tsinghua University, Beijing, 100084 China
    Stem Cell Res Ther 1:33. 2010
    ..Such differences and their underlying developmental pathways require clear definition and have major impacts on the preclinical test bed of pluripotent cells...
  7. pmc Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells
    Veronika Akopian
    The Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    In Vitro Cell Dev Biol Anim 46:247-58. 2010
    ....
  8. ncbi request reprint Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: opposite sides of the same coin
    P W Andrews
    The Centre for Stem Cell Biology and Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Biochem Soc Trans 33:1526-30. 2005
    ..In these features, the 'adapted' ES cells resembled malignant EC cells. The results suggest that ES cells may develop in culture in ways that mimic changes occurring in EC cells during tumour progression...
  9. ncbi request reprint The International Stem Cell Initiative: toward benchmarks for human embryonic stem cell research
    Peter W Andrews
    Department of Biomedical Sciences, University of Sheffield, Sheffield S10 2TN, UK
    Nat Biotechnol 23:795-7. 2005
  10. doi request reprint Pinacidil enhances survival of cryopreserved human embryonic stem cells
    Ivana Barbaric
    Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield, UK
    Cryobiology 63:298-305. 2011
    ..It is also considerably cheaper than Y-27632. Thus, the use of pinacidil offers an efficient method for recovery of cryopreserved dissociated human ES cells...
  11. doi request reprint Retinoic acid directs neuronal differentiation of human pluripotent stem cell lines in a non-cell-autonomous manner
    Peter D Tonge
    Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, Sheffield, UK
    Differentiation 80:20-30. 2010
    ..These data indicate that RA mediated neuronal differentiation of human EC and ES cell lines is not a cell autonomous program but comprises of a multi-staged program that requires intercellular input...
  12. pmc Prepatterning in the stem cell compartment
    Peter D Tonge
    Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 5:e10901. 2010
    ..Our results suggest that pluripotent NTERA2 stem cells oscillate between functionally distinct substates that are primed to select distinct lineages when differentiation is induced...
  13. pmc CD30 expression reveals that culture adaptation of human embryonic stem cells can occur through differing routes
    Neil J Harrison
    Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield, UK
    Stem Cells 27:1057-65. 2009
    ..In this cell line, CD30 expression did not segregate the normal and abnormal cells, and abnormal cells were not protected from apoptosis. These data suggest that culture adaptation can occur through a variety of mechanisms...
  14. ncbi request reprint Adaptation to culture of human embryonic stem cells and oncogenesis in vivo
    Duncan E C Baker
    Sheffield Regional Cytogenetics Service, Sheffield Children s Trust, Western Bank, Sheffield S10 2TH, UK
    Nat Biotechnol 25:207-15. 2007
    ..Identifying the genes critical for culture adaptation may thus reveal key players for both stem cell maintenance in vitro and germ cell tumorigenesis in vivo...
  15. doi request reprint Novel regulators of stem cell fates identified by a multivariate phenotype screen of small compounds on human embryonic stem cell colonies
    Ivana Barbaric
    Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield, UK
    Stem Cell Res 5:104-19. 2010
    ..The analysis of overlapping targets of pinacidil and the other survival compounds revealed that activity of PRK2, ROCK, MNK1, RSK1, and MSK1 kinases may contribute to the survival of hESCs...
  16. ncbi request reprint Cell-cell signaling through NOTCH regulates human embryonic stem cell proliferation
    Victoria Fox
    Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom
    Stem Cells 26:715-23. 2008
    ..The results indicate that cell-cell signaling through the NOTCH system provides a critical cue for the proliferation of human EC and ES cell in vitro...
  17. ncbi request reprint Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells
    Maryam M Matin
    The Centre for Stem Cell Biology, University of Sheffield, Western Bank, S10 2TN, U K
    Stem Cells 22:659-68. 2004
    ..NTERA2 hEC cells also differentiated, but not to trophectoderm, suggesting their equivalence to a later stage of embryogenesis than other hEC and hES cells...
  18. ncbi request reprint The CDK inhibitor p27 enhances neural differentiation in pluripotent NTERA2 human EC cells, but does not permit differentiation of 2102Ep nullipotent human EC cells
    Ahmad R Bahrami
    Department of Biomedical Science, The Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Mech Dev 122:1034-42. 2005
    ....
  19. ncbi request reprint Culture adaptation of embryonic stem cells echoes germ cell malignancy
    Neil J Harrison
    Centre for Stem Cell Biology and the Department of Biomedical Science, University of Sheffield, Sheffield, UK
    Int J Androl 30:275-81; discussion 281. 2007
    ..Understanding the mechanisms that drive such culture adaptation of ES cells may also provide insights into the development and progression of GCT...
  20. doi request reprint Modeling the evolution of culture-adapted human embryonic stem cells
    Victor Olariu
    Department of Automatic Control and Systems Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK
    Stem Cell Res 4:50-6. 2010
    ..In particular, it highlights the effect of population size, suggesting that the maintenance of cells in small populations reduces the likelihood that abnormal cultures will develop...
  21. pmc Specific knockdown of OCT4 in human embryonic stem cells by inducible short hairpin RNA interference
    Gaetano Zafarana
    Department of Biomedical Science, Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield, United Kingdom
    Stem Cells 27:776-82. 2009
    ..stem cells 2009;27:776-782...
  22. doi request reprint High-content screening for chemical modulators of embryonal carcinoma cell differentiation and survival
    Ivana Barbaric
    Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, UK
    J Biomol Screen 16:603-17. 2011
    ..The effects of rottlerin were also investigated in human ES cells. Rottlerin inhibited the self-renewal ability of ES cells, caused the cell cycle arrest, and repressed the expression of pluripotency-associated genes...
  23. doi request reprint The role of SMAD4 in human embryonic stem cell self-renewal and stem cell fate
    Stuart Avery
    Department of Biomedical Science, Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield, S10 2TN, United Kingdom
    Stem Cells 28:863-73. 2010
    ..These results suggest that SMAD4 is not required for maintenance of the undifferentiated state of hESC, but rather to stabilize that state...
  24. ncbi request reprint Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells
    Jonathan S Draper
    Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Nat Biotechnol 22:53-4. 2004
    ..These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental...
  25. pmc Restoration of auditory evoked responses by human ES-cell-derived otic progenitors
    Wei Chen
    Centre for Stem Cell Biology, University of Sheffield, Sheffield S10 2TN, UK
    Nature 490:278-82. 2012
    ..These results should stimulate further research into the development of a cell-based therapy for deafness...
  26. doi request reprint New insights into the control of stem cell pluripotency
    Paul J Gokhale
    Centre for Stem Cell Biology, Department of Biomedical Science, The University of Sheffield, Sheffield S10 2TN, UK
    Cell Stem Cell 2:4-5. 2008
    ..New research published recently in Nature (Chambers et al., 2007) points to an unexpected role for Nanog in the maintenance of pluripotency in mouse ES cells...
  27. pmc STELLA facilitates differentiation of germ cell and endodermal lineages of human embryonic stem cells
    Patompon Wongtrakoongate
    Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 8:e56893. 2013
    ..Taken together, this finding suggests a role for STELLA in facilitating germline and endodermal differentiation of human ES cells...
  28. doi request reprint The development of pluripotent stem cells
    Paul J Gokhale
    Centre for Stem Cell Biology and the Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK
    Curr Opin Genet Dev 22:403-8. 2012
    ..More recently, the recognition of genetic change and culture adaptation of these cells after prolonged culture has returned us to those cancer roots...
  29. doi request reprint Human embryonic stem cells fail to activate CHK1 and commit to apoptosis in response to DNA replication stress
    Joëlle A Desmarais
    Centre for Stem Cell Biology, Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, United Kingdom
    Stem Cells 30:1385-93. 2012
    ....
  30. doi request reprint Human embryonic stem cells: 10 years on
    Paul J Gokhale
    Department of Biomedical Science, Centre for Stem Cell Biology, University of Sheffield, Sheffield, UK
    Lab Invest 89:259-62. 2009
    ..The recent appearance of technology to convert somatic cells to 'induced Pluripotent Stem Cells' closely resembling ES cells will certainly speed up these developments...
  31. doi request reprint Human fetal auditory stem cells can be expanded in vitro and differentiate into functional auditory neurons and hair cell-like cells
    Wei Chen
    Centre for Stem Cell Biology, University of Sheffield, Sheffield, UK
    Stem Cells 27:1196-204. 2009
    ....
  32. doi request reprint High-content screening of small compounds on human embryonic stem cells
    Ivana Barbaric
    Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Biochem Soc Trans 38:1046-50. 2010
    ....
  33. ncbi request reprint mGluR5 is involved in dendrite differentiation and excitatory synaptic transmission in NTERA2 human embryonic carcinoma cell-derived neurons
    Hyeonseon Park
    Biomedical Science, School of Medicine and Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK
    Neuropharmacology 52:1403-14. 2007
    ..Thus, mGluR5 activity at early stages of differentiation is likely to play a role in the development of multipotent cell-derived neurons...
  34. doi request reprint Altered patterns of differentiation in karyotypically abnormal human embryonic stem cells
    Alireza Fazeli
    The University of Sheffield, Academic Unit of Reproductive and Developmental Medicine, Sheffield, UK
    Int J Dev Biol 55:175-80. 2011
    ....
  35. pmc Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage
    Katherine Amps
    Centre for Stem Cell Biology, Department of Biomedical Science, The University of Sheffield, Sheffield, UK
    Nat Biotechnol 29:1132-44. 2011
    ..21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells...
  36. doi request reprint Functionally defined substates within the human embryonic stem cell compartment
    Peter D Tonge
    Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, UK
    Stem Cell Res 7:145-53. 2011
    ..These data suggest that ES cells with pluripotent/self-renewal capacities can exhibit different responses to induction of differentiation...
  37. ncbi request reprint A prospective on stem cell research
    Paul J Gokhale
    The Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, United Kingdom
    Semin Reprod Med 24:289-97. 2006
    ....
  38. ncbi request reprint Transient and stable transgene expression in human embryonic stem cells
    Chee Gee Liew
    Centre for Stem Cell Biology, Department of Biomedical Science, Western Bank, University of Sheffield, Sheffield S10 2TN, United Kingdom
    Stem Cells 25:1521-8. 2007
    ..Our data also demonstrate that pUbiC, pR26, pCAGG, and pCAG are more superior to the pCMV for generation of stable transfectants in hES cells. Disclosure of potential conflicts of interest is found at the end of this article...
  39. doi request reprint Surface marker antigens in the characterization of human embryonic stem cells
    Andrew J Wright
    BioServ, The University of Sheffield, Sheffield S10 2TN, UK
    Stem Cell Res 3:3-11. 2009
    ..Many of these were first identified using both mouse and human EC cells, although the cell surface antigen phenotype of human EC and ES cells has proved to be significantly different from that of murine EC and ES cells...
  40. ncbi request reprint Hybrids of pluripotent and nullipotent human embryonal carcinoma cells: partial retention of a pluripotent phenotype
    C Duran
    Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, United Kingdom
    Int J Cancer 93:324-32. 2001
    ..Thus, the mechanisms that restrict the differentiation capacity of the nullipotent 2102Ep line are complex and include both recessive and dominant acting factors...
  41. ncbi request reprint Cytotrophoblast stem cell lines derived from human embryonic stem cells and their capacity to mimic invasive implantation events
    R Harun
    Centre for Stem Cell Biology, University of Sheffield, Western Bank, Sheffield, UK
    Hum Reprod 21:1349-58. 2006
    ..Research is severely constrained by ethical and practical considerations; therefore, we aimed to generate cytotrophoblast stem (CTBS) cell lines from human embryonic stem cells (HESCs)...
  42. ncbi request reprint Embryonic stem cells: advances toward potential therapeutic use
    Jonathan S Draper
    Department of Biomedical Science, University of Sheffield, Sheffield, UK
    Curr Opin Obstet Gynecol 14:309-15. 2002
    ..Recent studies have focused on identifying method for culture of these cells and inducing their differentiation into specific cell types...
  43. ncbi request reprint Brachyury is expressed by human teratocarcinoma cells in the absence of mesodermal differentiation
    P J Gokhale
    Department of Biomedical Science, University of Sheffield, United Kingdom
    Cell Growth Differ 11:157-62. 2000
    ..Thus, in human teratocarcinomas, Brachyury expression is not necessarily an indicator of commitment to mesodermal differentiation...
  44. ncbi request reprint Dysfunction of the mitotic:meiotic switch as a potential cause of neoplastic conversion of primordial germ cells
    D J B Adamah
    Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, UK
    Int J Androl 29:219-27. 2006
    ..One possibility is that abnormal regulation of such proteins involved in the synaptonemal complex could also lead to genetic instability in PGC and so also initiate tumour development...
  45. ncbi request reprint Meeting summary: European Workshop on Stem Cells, European Centre for the Validation of Biomedical Testing Methods, Institute for Health and Consumer Protection, Joint Research Centre, Ispra, Italy, November 21-23, 2001
    Laura Gribaldo
    European Centre for the Validation of Biomedical Testing Methods, Institute for Health and Consumer Protection, Joint Research Centre, Ispra, Italy
    Exp Hematol 30:628-33. 2002
  46. ncbi request reprint Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells
    Tariq Enver
    MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, UK
    Hum Mol Genet 14:3129-40. 2005
    ..As this balance is also an essential feature of cancer, the mechanisms of culture adaptation may mirror those of oncogenesis and tumour progression...
  47. ncbi request reprint Reprogramming in inter-species embryonal carcinoma-somatic cell hybrids induces expression of pluripotency and differentiation markers
    Marzena Flasza
    Intercytex Ltd, Manchester, United Kingdom
    Cloning Stem Cells 5:339-54. 2003
    ....
  48. ncbi request reprint LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells
    Laurence Daheron
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
    Stem Cells 22:770-8. 2004
    ..Feeder-free culture conditions that maintain hESCs in an undifferentiated state do not show activation of STAT3, suggesting that distinct signaling mechanisms govern the self-renewal of hESCs...
  49. ncbi request reprint Differentiation of human embryonal carcinomas in vitro and in vivo reveals expression profiles relevant to normal development
    Rolf I Skotheim
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital and University of Oslo, Norway
    Cancer Res 65:5588-98. 2005
    ....
  50. ncbi request reprint The selfish stem cell
    Peter W Andrews
    Nat Biotechnol 24:325-6. 2006
  51. ncbi request reprint Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research
    Richard Josephson
    GlobalStem, Inc, 6 Taft Court, Rockville, Maryland 20850, USA
    Stem Cells 25:437-46. 2007
    ..On the basis of global gene and microRNA expression, 2102Ep is somewhat less similar to hESC than the alternatives; however, 2102Ep expresses more hESC-associated microRNAs than NTERA-2 does, and fewer markers of differentiated fates...
  52. ncbi request reprint Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
    Oluseun Adewumi
    UK Stem Cell Bank, Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Herts, EN6 3QG, UK
    Nat Biotechnol 25:803-16. 2007
    ..Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected...
  53. ncbi request reprint The terminology of teratocarcinomas and teratomas
    Ivan Damjanov
    Nat Biotechnol 25:1212; discussion 1212. 2007
  54. pmc PAX4 enhances beta-cell differentiation of human embryonic stem cells
    Chee Gee Liew
    Centre for Stem Cell Biology, Department of Biomedical Science, The University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 3:e1783. 2008
    ....