Enrique Amaya

Summary

Affiliation: University of Manchester
Country: UK

Publications

  1. pmc Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis
    Shoko Ishibashi
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Biol Open 1:1273-6. 2012
  2. ncbi request reprint Xenomics
    Enrique Amaya
    The Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, CB2 1QN, United Kingdom
    Genome Res 15:1683-91. 2005
  3. pmc Amputation-induced reactive oxygen species are required for successful Xenopus tadpole tail regeneration
    Nick R Love
    Faculty of Life Sciences, University of Manchester, UK
    Nat Cell Biol 15:222-8. 2013
  4. pmc Characterisation of a new regulator of BDNF signalling, Sprouty3, involved in axonal morphogenesis in vivo
    Niki Panagiotaki
    Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester, UK
    Development 137:4005-15. 2010
  5. pmc Inositol kinase and its product accelerate wound healing by modulating calcium levels, Rho GTPases, and F-actin assembly
    Ximena Soto
    Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom
    Proc Natl Acad Sci U S A 110:11029-34. 2013
  6. pmc Temporal and spatial expression of FGF ligands and receptors during Xenopus development
    Robert Lea
    The Healing Foundation Centre, Michael Smith Building, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Dev Dyn 238:1467-79. 2009
  7. ncbi request reprint A secretory cell type develops alongside multiciliated cells, ionocytes and goblet cells, and provides a protective, anti-infective function in the frog embryonic mucociliary epidermis
    Eamon Dubaissi
    Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Development 141:1514-25. 2014
  8. pmc C/EBPalpha initiates primitive myelopoiesis in pluripotent embryonic cells
    Yaoyao Chen
    Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Blood 114:40-8. 2009
  9. pmc BDNF promotes target innervation of Xenopus mandibular trigeminal axons in vivo
    Jeffrey K Huang
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
    BMC Dev Biol 7:59. 2007
  10. ncbi request reprint A simple method of transgenesis using I-SceI meganuclease in Xenopus
    Shoko Ishibashi
    The Healing Foundation Centre, The Faculty of Life Sciences, University of Manchester, Manchester, England, UK
    Methods Mol Biol 917:205-18. 2012

Collaborators

Detail Information

Publications39

  1. pmc Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis
    Shoko Ishibashi
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Biol Open 1:1273-6. 2012
    ..These findings have far reaching implications in our quest to develop efficient reverse genetic approaches in this emerging amphibian model...
  2. ncbi request reprint Xenomics
    Enrique Amaya
    The Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, CB2 1QN, United Kingdom
    Genome Res 15:1683-91. 2005
    ....
  3. pmc Amputation-induced reactive oxygen species are required for successful Xenopus tadpole tail regeneration
    Nick R Love
    Faculty of Life Sciences, University of Manchester, UK
    Nat Cell Biol 15:222-8. 2013
    ..7), which, in turn, is essential for proper tail regeneration. These findings demonstrate that injury-induced ROS production is an important regulator of tissue regeneration...
  4. pmc Characterisation of a new regulator of BDNF signalling, Sprouty3, involved in axonal morphogenesis in vivo
    Niki Panagiotaki
    Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester, UK
    Development 137:4005-15. 2010
    ....
  5. pmc Inositol kinase and its product accelerate wound healing by modulating calcium levels, Rho GTPases, and F-actin assembly
    Ximena Soto
    Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, United Kingdom
    Proc Natl Acad Sci U S A 110:11029-34. 2013
    ....
  6. pmc Temporal and spatial expression of FGF ligands and receptors during Xenopus development
    Robert Lea
    The Healing Foundation Centre, Michael Smith Building, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Dev Dyn 238:1467-79. 2009
    ..Developmental Dynamics 238:1467-1479, 2009. (c) 2009 Wiley-Liss, Inc...
  7. ncbi request reprint A secretory cell type develops alongside multiciliated cells, ionocytes and goblet cells, and provides a protective, anti-infective function in the frog embryonic mucociliary epidermis
    Eamon Dubaissi
    Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Development 141:1514-25. 2014
    ....
  8. pmc C/EBPalpha initiates primitive myelopoiesis in pluripotent embryonic cells
    Yaoyao Chen
    Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Blood 114:40-8. 2009
    ..Thus, C/EPBalpha is the first known single factor capable of initiating an entire myelopoiesis pathway in pluripotent cells in the embryo...
  9. pmc BDNF promotes target innervation of Xenopus mandibular trigeminal axons in vivo
    Jeffrey K Huang
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
    BMC Dev Biol 7:59. 2007
    ..Brain derived neurotrophic factor, BDNF is known to stimulate axon outgrowth and branching. The goal of this study is to determine whether BDNF functions as the proposed target recognition signal in the Xenopus cement gland...
  10. ncbi request reprint A simple method of transgenesis using I-SceI meganuclease in Xenopus
    Shoko Ishibashi
    The Healing Foundation Centre, The Faculty of Life Sciences, University of Manchester, Manchester, England, UK
    Methods Mol Biol 917:205-18. 2012
    ....
  11. pmc ERK and phosphoinositide 3-kinase temporally coordinate different modes of actin-based motility during embryonic wound healing
    Jingjing Li
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    J Cell Sci 126:5005-17. 2013
    ..These findings reveal a new mechanism for coordinating different modes of actin-based motility in a complex tissue setting, namely embryonic wound healing...
  12. ncbi request reprint Expression cloning screening of a unique and full-length set of cDNA clones is an efficient method for identifying genes involved in Xenopus neurogenesis
    Jana Voigt
    Department of Anatomy, University of Cambridge, Downing Site, Cambridge CB2 3DY, UK
    Mech Dev 122:289-306. 2005
    ..These results demonstrate the effectiveness of a unique full-length set of cDNA clones for uncovering players in a developmental pathway...
  13. pmc pTransgenesis: a cross-species, modular transgenesis resource
    Nick R Love
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, UK
    Development 138:5451-8. 2011
    ..Thus, the pTransgenesis resource fosters a cross-model standardization of commonly used transgenesis elements, streamlines DNA construct creation and facilitates collaboration between researchers working on different model organisms...
  14. pmc Genome-wide analysis of gene expression during Xenopus tropicalis tadpole tail regeneration
    Nick R Love
    Faculty of Life Sciences, University of Manchester, UK
    BMC Dev Biol 11:70. 2011
    ..Here, we explore tadpole tail regeneration in Xenopus tropicalis, a diploid frog with a sequenced genome...
  15. ncbi request reprint FGF signal interpretation is directed by Sprouty and Spred proteins during mesoderm formation
    Jeremy M Sivak
    The Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge
    Dev Cell 8:689-701. 2005
    ..These differences, combined with the timing of their developmental expression, suggest a mechanism to switch FGFR signal interpretation to coordinate mesoderm formation and cell movements during gastrulation...
  16. ncbi request reprint Carbohydrate metabolism during vertebrate appendage regeneration: what is its role? How is it regulated?: A postulation that regenerating vertebrate appendages facilitate glycolytic and pentose phosphate pathways to fuel macromolecule biosynthesis
    Nick R Love
    Department of Molecular Biology, University of Bergen, Bergen, Norway The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester, UK Laboratory for Organogenesis and Neurogenesis, RIKEN Center for Developmental Biology, Chuo Ku, Kobe, Japan
    Bioessays 36:27-33. 2014
    ..We suggest that this metabolic shift is integral to the appendage regeneration program and that the Xenopus model is a powerful experimental system to further explore this phenomenon. Also watch the Video Abstract. ..
  17. pmc spib is required for primitive myeloid development in Xenopus
    Ricardo M B Costa
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Blood 112:2287-96. 2008
    ..Thus, we conclude that spib sits at the top of the known genetic hierarchy that leads to the specification of primitive myeloid cells in amphibians...
  18. doi request reprint Labeling primitive myeloid progenitor cells in Xenopus
    Ricardo Costa
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester, UK
    Methods Mol Biol 916:141-55. 2012
    ..Here we describe two methods for labeling primitive myeloid progenitor cells during early Xenopus development...
  19. ncbi request reprint Reverse genetic studies using antisense morpholino oligonucleotides
    Yanan Zhao
    The Healing Foundation Centre, The Faculty of Life Sciences, University of Manchester, Manchester, England, UK
    Methods Mol Biol 917:143-54. 2012
    ..The protocol we describe here is for both Xenopus laevis and Xenopus tropicalis...
  20. pmc Generating transgenic frog embryos by restriction enzyme mediated integration (REMI)
    Shoko Ishibashi
    The Healing Foundation Centre, The Faculty of Life Sciences, University of Manchester, Manchester, England, UK
    Methods Mol Biol 917:185-203. 2012
    ..This procedure generates non-mosaic transgenic embryos at high frequency and efficiency...
  21. pmc A functional genome-wide in vivo screen identifies new regulators of signalling pathways during early Xenopus embryogenesis
    Siwei Zhang
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    PLoS ONE 8:e79469. 2013
    ....
  22. pmc Maintenance of motor neuron progenitors in Xenopus requires a novel localized cyclin
    Jun An Chen
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    EMBO Rep 8:287-92. 2007
    ..Thus, we have identified, for the first time, a tissue-specific cell-cycle regulator that is essential for the maintenance of a pool of neural progenitors in the vertebrate spinal cord...
  23. pmc FGF signalling: diverse roles during early vertebrate embryogenesis
    Karel Dorey
    The Healing Foundation Centre, Michael Smith Building, Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK
    Development 137:3731-42. 2010
    ..Here, we summarise our current understanding of the regulation and roles of FGF signalling during early vertebrate development...
  24. ncbi request reprint Tadpole tail regeneration in Xenopus
    Yaoyao Chen
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, U K
    Biochem Soc Trans 42:617-23. 2014
    ..In the present article, we briefly examine the genetic, molecular and cellular mechanisms underlying the regeneration of the Xenopus tadpole tail. ..
  25. pmc cis-Regulatory remodeling of the SCL locus during vertebrate evolution
    Berthold Gottgens
    Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    Mol Cell Biol 30:5741-51. 2010
    ..Our data demonstrate remarkable cis-regulatory remodelling across the SCL locus and indicate that stable patterns of expression can mask extensive regulatory change. These insights illuminate our understanding of vertebrate evolution...
  26. ncbi request reprint A study of mesoderm patterning through the analysis of the regulation of Xmyf-5 expression
    Matthew Polli
    Wellcome Trust Cancer Research UK Institute, Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Development 129:2917-27. 2002
    ..Furthermore, the identification of HBX2 provides a tool with which to identify other molecules involved in the regulation of Xmyf-5 expression during gastrulation...
  27. doi request reprint Generation of Transgenic Xenopus laevis: III. Sperm Nuclear Transplantation
    Shoko Ishibashi
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    CSH Protoc 2007:pdb.prot4840. 2007
    ..Because the transgene integrates into the genome prior to fertilization, the resulting transgenic embryos are not chimeric and there is no need to breed to the next generation in order to obtain nonmosaic transgenic animals...
  28. doi request reprint Generation of Transgenic Xenopus laevis: II. Sperm Nuclei Preparation
    Shoko Ishibashi
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    CSH Protoc 2007:pdb.prot4839. 2007
    ..Sperm suspensions are prepared by filtration and centrifugation, and then treated with lysolecithin to disrupt the plasma membrane of the cells. Sperm nuclei can be stored frozen in small aliquots at -80°C...
  29. doi request reprint Generation of Transgenic Xenopus laevis: I. High-Speed Preparation of Egg Extracts
    Shoko Ishibashi
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    CSH Protoc 2007:pdb.prot4838. 2007
    ..This fraction promotes swelling of sperm nuclei, but does not promote DNA replication. By adding the egg extract to the reaction, the sperm chromatin partially decondenses, facilitating integration of plasmid DNA into the genome...
  30. ncbi request reprint Techniques and probes for the study of Xenopus tropicalis development
    Mustafa K Khokha
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, USA
    Dev Dyn 225:499-510. 2002
    ..We show that MOs function in X. tropicalis. Finally, X. tropicalis offers the possibility for forward genetics and genomic analysis...
  31. ncbi request reprint Molecular components of the endoderm specification pathway in Xenopus tropicalis
    Anjali D'Souza
    Cincinnati Children s Hospital Medical Center, Division of Developmental Biology, Cincinnati, Ohio 45229 3039, USA
    Dev Dyn 226:118-27. 2003
    ....
  32. ncbi request reprint Novel gene expression domains reveal early patterning of the Xenopus endoderm
    Ricardo M B Costa
    Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, Tennis Court Road, Cambridge, CB2 1QR, UK
    Gene Expr Patterns 3:509-19. 2003
    ..These new genes provide valuable tools for studying endodermal patterning and organogenesis in Xenopus...
  33. ncbi request reprint Pilot morpholino screen in Xenopus tropicalis identifies a novel gene involved in head development
    Sue Kenwrick
    Cambridge Institute for Medical Research, Hills Road, Cambridge United Kingdom
    Dev Dyn 229:289-99. 2004
    ..Together with the early embryonic expression in the anterior neural plate, these data indicate that pinhead is a novel gene involved in controlling head development...
  34. pmc Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system
    Katharine O Hartley
    Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Proc Natl Acad Sci U S A 99:1377-82. 2002
    ..Finally, we exploit this system to characterize the neural and mesodermal defects obtained from early misexpression of Xvent-2. These results emphasize the potential of this system for the controlled analyses of gene function in Xenopus...
  35. ncbi request reprint Identification of novel genes affecting mesoderm formation and morphogenesis through an enhanced large scale functional screen in Xenopus
    Jun An Chen
    Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK
    Mech Dev 122:307-31. 2005
    ..We determined the expression pattern of the 82 genes and found that most of the genes were regionalized and expressed in mesoderm. We expect that many of the genes identified in this screen will be important in mesoderm formation...
  36. ncbi request reprint A Xenopus tropicalis oligonucleotide microarray works across species using RNA from Xenopus laevis
    Andrew D Chalmers
    Wellcome Trust Cancer Research UK Gurdon Institute, Tennis Court Road, CB2 1QR, Cambridge, UK
    Mech Dev 122:355-63. 2005
    ..tropicalis set of experiments was only 12% less than the overlap between two sets of X. tropicalis experiments. Therefore researchers can work with X. laevis and still make use of the advantages offered by X. tropicalis microarrays...
  37. ncbi request reprint Local tissue interactions across the dorsal midline of the forebrain establish CNS laterality
    Miguel L Concha
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Neuron 39:423-38. 2003
    ..From these data, we propose that laterality is determined by a competitive interaction between the left and right epithalamus and that Nodal signaling biases the outcome of this competition...
  38. ncbi request reprint 1.15 A crystal structure of the X. tropicalis Spred1 EVH1 domain suggests a fourth distinct peptide-binding mechanism within the EVH1 family
    Nicholas J Harmer
    Department of Biochemistry, 80 Tennis Court Road, Cambridge, CB2 1GA, UK
    FEBS Lett 579:1161-6. 2005
    ..We propose that Spred1 will bind peptides that are less proline-rich than other EVH1 domains, with conformational changes indicating an induced fit...
  39. ncbi request reprint Defining a large set of full-length clones from a Xenopus tropicalis EST project
    Michael J Gilchrist
    Wellcome Trust Cancer Research UK Gurdon Institute, Cambridge CB21QR, UK
    Dev Biol 271:498-516. 2004
    ..The creation of the unique set of expressed sequences and the FL clone set pave the way toward a large-scale systematic analysis of gene sequence, gene expression, and gene function in this vertebrate species...