Dirk Werling

Summary

Affiliation: Royal Veterinary College
Country: UK

Publications

  1. pmc Differential production of cytokines, reactive oxygen and nitrogen by bovine macrophages and dendritic cells stimulated with Toll-like receptor agonists
    Dirk Werling
    Institute of Veterinary Virology, University of Bern, CH 3012 Bern, Switzerland
    Immunology 111:41-52. 2004
  2. pmc Adaptive evolution of Toll-like receptor 5 in domesticated mammals
    Sarah A Smith
    School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington LE12 5RD, UK
    BMC Evol Biol 12:122. 2012
  3. pmc The bovine chemokine receptors and their mRNA abundance in mononuclear phagocytes
    Stephanie Widdison
    Institute for Animal Health, Compton, Newbury, Berkshire, UK
    BMC Genomics 11:439. 2010
  4. pmc Molecular evolution of bovine Toll-like receptor 2 suggests substitutions of functional relevance
    Oliver C Jann
    The Roslin Institute and Royal Dick School of Veterinary Studies, University of Edinburgh, Roslin Biocentre, Midlothian, UK
    BMC Evol Biol 8:288. 2008
  5. ncbi request reprint Ability to differentiate between cp and ncp BVDV by microarrays: towards an application in clinical veterinary medicine?
    Dirk Werling
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Vet Immunol Immunopathol 108:157-64. 2005
  6. ncbi request reprint Pattern recognition receptors in companion and farm animals - the key to unlocking the door to animal disease?
    Dirk Werling
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Vet J 174:240-51. 2007
  7. doi request reprint Variation matters: TLR structure and species-specific pathogen recognition
    Dirk Werling
    Royal Veterinary College Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield, AL9 7TA, UK
    Trends Immunol 30:124-30. 2009
  8. ncbi request reprint Innate immunity and vaccination
    Dirk Werling
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Vet Immunol Immunopathol 112:1. 2006
  9. ncbi request reprint Expression of TOLL-like receptors (TLR) by bovine antigen-presenting cells-potential role in pathogen discrimination?
    Dirk Werling
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Vet Immunol Immunopathol 112:2-11. 2006
  10. ncbi request reprint Binding and entry of respiratory syncytial virus into host cells and initiation of the innate immune response
    James Harris
    Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK
    Cell Microbiol 5:671-80. 2003

Collaborators

Detail Information

Publications41

  1. pmc Differential production of cytokines, reactive oxygen and nitrogen by bovine macrophages and dendritic cells stimulated with Toll-like receptor agonists
    Dirk Werling
    Institute of Veterinary Virology, University of Bern, CH 3012 Bern, Switzerland
    Immunology 111:41-52. 2004
    ..Collectively, our results point to profound differences in pathogen-derived signal-response coupling occurring commensurate with distinct functions carried out by Mphi or DC...
  2. pmc Adaptive evolution of Toll-like receptor 5 in domesticated mammals
    Sarah A Smith
    School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington LE12 5RD, UK
    BMC Evol Biol 12:122. 2012
    ..To determine if this is the case, we examined the extent of adaptive evolution in the TLR5 gene in both individual species and defined clades of the mammalia...
  3. pmc The bovine chemokine receptors and their mRNA abundance in mononuclear phagocytes
    Stephanie Widdison
    Institute for Animal Health, Compton, Newbury, Berkshire, UK
    BMC Genomics 11:439. 2010
    ..Although chemokine receptors have been well characterised in humans, the chemokine receptor repertoire of cattle is not well characterised and many sequences are yet to be experimentally validated...
  4. pmc Molecular evolution of bovine Toll-like receptor 2 suggests substitutions of functional relevance
    Oliver C Jann
    The Roslin Institute and Royal Dick School of Veterinary Studies, University of Edinburgh, Roslin Biocentre, Midlothian, UK
    BMC Evol Biol 8:288. 2008
    ....
  5. ncbi request reprint Ability to differentiate between cp and ncp BVDV by microarrays: towards an application in clinical veterinary medicine?
    Dirk Werling
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Vet Immunol Immunopathol 108:157-64. 2005
    ..These data will help to further understand our knowledge regarding BVDV infection, and will especially help to understand differences in cellular responses to cpe and ncp biotypes...
  6. ncbi request reprint Pattern recognition receptors in companion and farm animals - the key to unlocking the door to animal disease?
    Dirk Werling
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Vet J 174:240-51. 2007
    ..In this review, current knowledge regarding these receptors in species of veterinary relevance is summarised...
  7. doi request reprint Variation matters: TLR structure and species-specific pathogen recognition
    Dirk Werling
    Royal Veterinary College Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield, AL9 7TA, UK
    Trends Immunol 30:124-30. 2009
    ..Here, we aim to summarize our current knowledge of species-specific TLR responses and briefly discuss that vaccine efficacy in relevant host species might be improved by considering the species-specific TLR responses...
  8. ncbi request reprint Innate immunity and vaccination
    Dirk Werling
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Vet Immunol Immunopathol 112:1. 2006
  9. ncbi request reprint Expression of TOLL-like receptors (TLR) by bovine antigen-presenting cells-potential role in pathogen discrimination?
    Dirk Werling
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Vet Immunol Immunopathol 112:2-11. 2006
    ....
  10. ncbi request reprint Binding and entry of respiratory syncytial virus into host cells and initiation of the innate immune response
    James Harris
    Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK
    Cell Microbiol 5:671-80. 2003
    ..This review summarizes our current understanding of RSV binding and uptake in mammalian cells and how these initial interactions influence the subsequent innate immune response generated...
  11. pmc Inflamm-aging and arachadonic acid metabolite differences with stage of tendon disease
    Stephanie Georgina Dakin
    Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hatfield, United Kingdom
    PLoS ONE 7:e48978. 2012
    ....
  12. pmc Potential evidence for biotype-specific chemokine profile following BVDV infection of bovine macrophages
    Stephen Burr
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Vet Immunol Immunopathol 150:123-7. 2012
    ..This study helps to further our understanding of the pathogenesis of BVDV infection, highlighting biotype-specific cellular responses...
  13. ncbi request reprint TOLL-like receptors linking innate and adaptive immune response
    Dirk Werling
    Institute of Veterinary Virology, University of Berne, Länggass Str 122, CH 3012 Bern, Switzerland
    Vet Immunol Immunopathol 91:1-12. 2003
    ....
  14. doi request reprint Species-specific PAMP recognition by TLR2 and evidence for species-restricted interaction with Dectin-1
    Sam Willcocks
    Department of Pathology and Infectious Diseases, Royal Veterinary College, Hatfield, United Kingdom
    J Leukoc Biol 94:449-58. 2013
    ....
  15. doi request reprint Expression of trefoil factor genes in the duodenum and colon of dogs with inflammatory bowel disease and healthy dogs
    Silke Schmitz
    Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hawkshead Campus, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire, AL9 7TA, United Kingdom
    Vet Immunol Immunopathol 151:168-72. 2013
    ..Whether this is a cause or consequence of IBD could not be established...
  16. doi request reprint Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response
    Amanda Gibson
    The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, UK
    Dev Comp Immunol 36:332-41. 2012
    ....
  17. ncbi request reprint Caveolae and caveolin in immune cells: distribution and functions
    James Harris
    Sir William Dunn School of Pathology, South Parks Road, OX1 3RE, Oxford, UK
    Trends Immunol 23:158-64. 2002
    ..Here, we review the distribution and role of caveolae and caveolin in mammalian immune cells...
  18. doi request reprint Host species-specific usage of the TLR4-LPS receptor complex
    Regina Lizundia
    Royal Veterinary College, Hawkshead Campus, Hatfield, UK
    Innate Immun 14:223-31. 2008
    ..However, no clear role for the RSV F protein of either human or bovine RSV alone in stimulating TLR4-dependent NF-kappaB activation was observed...
  19. pmc Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus
    Shan Herath
    Royal Veterinary College, Department of Veterinary Clinical Sciences, University of London, North Mymms, Hatfield, Hertfordshire AL9 7TA, United Kingdom
    Endocrinology 147:562-70. 2006
    ..In conclusion, epithelial and stromal cells detect and respond to bacteria, which modulate their endocrine function...
  20. doi request reprint Comparison of TNFα responses induced by Toll-like receptor ligands and probiotic Enterococcus faecium in whole blood and peripheral blood mononuclear cells of healthy dogs
    Silke Schmitz
    Royal Veterinary College London, Department of Veterinary Clinical Sciences, Hawkshead Lane, North Mymms, AL9 7TA, United Kingdom
    Vet Immunol Immunopathol 153:170-4. 2013
    ..In conclusion, WB stimulation should be considered a valid alternative to PBMC stimulation in the canine system...
  21. doi request reprint Gene expression of selected signature cytokines of T cell subsets in duodenal tissues of dogs with and without inflammatory bowel disease
    Silke Schmitz
    Royal Veterinary College, University of London, Department of Veterinary Clinical Sciences, Hawkshead Campus, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK
    Vet Immunol Immunopathol 146:87-91. 2012
    ..Thus, no clear evidence for the involvement of Th-17 signature cytokines in canine IBD at the mRNA level could be shown. The contribution of specific T cell subsets to the pathogenesis of canine IBD warrants further investigation...
  22. doi request reprint Identification and functional characterization of a bovine orthologue to DC-SIGN
    Yoshika Yamakawa
    Department of Pathology and Infectious Diseases, Royal Veterinary College, Hatfield, UK
    J Leukoc Biol 83:1396-403. 2008
    ..Thus, there is evidence of a DC-SIGN-like molecule expressed specifically by bovine DC. This molecule may play an important role in the infection of bovine (DC) cells with M. bovis...
  23. pmc Polymorphisms in the TLR4 and TLR5 gene are significantly associated with inflammatory bowel disease in German shepherd dogs
    Aarti Kathrani
    Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hatfield, United Kingdom
    PLoS ONE 5:e15740. 2010
    ..Further studies are required to confirm the functional importance of these polymorphisms in the pathogenesis of this disease...
  24. doi request reprint Evaluation of the effect of phosphodiesterase on equine platelet activation and the effect of antigen challenge on platelet phosphodiesterase activity in horses with recurrent airway obstruction
    Bettina Dunkel
    Department of Veterinary Clinical Sciences, The Royal Veterinary College, North Mymms, Herts AL9 7TA, England
    Am J Vet Res 71:534-40. 2010
    ....
  25. doi request reprint Assessment and quantification of post-weaning multi-systemic wasting syndrome severity at farm level
    Pablo Alarcon
    Department of Veterinary Clinical Sciences, Royal Veterinary College, Hatfield AL9 7TA, United Kingdom
    Prev Vet Med 98:19-28. 2011
    ..5. The identified farm level PMWS severities will be used to identify risk factors related to these, to assess the efficacy of PCV2 vaccination and investigating the economic impact of potential control measures...
  26. doi request reprint Yeast-surface expressed BVDV E2 protein induces a Th1/Th2 response in naïve T cells
    Robert Patterson
    Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
    Dev Comp Immunol 37:107-14. 2012
    ..Our studies demonstrate a use for S. cerevisiae as a vehicle for transporting BVDV vaccine antigen to antigen-presenting cell in order to elicit cell-mediated immunity even in naïve animals...
  27. doi request reprint Toll-like receptors in domestic animals
    Thomas W Jungi
    Institute of Veterinary Virology, Vetsuisse Faculty, University of Bern, Laenggasstrasse 122, CH 3001 Bern, Switzerland
    Cell Tissue Res 343:107-20. 2011
    ..In a few cases, expression of a given amino acid in the extracellular (ligand-binding) portion of TLR members could be associated with infectious diseases. This will be discussed below...
  28. pmc Inhibition of cytosolic Phospholipase A2 prevents prion peptide-induced neuronal damage and co-localisation with Beta III Tubulin
    Victoria Last
    Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, UK
    BMC Neurosci 13:106. 2012
    ..Here we report the effects of the prion peptide fragment HuPrP106-126 on the PLA2 cascade in primary cortical neurons and translocation of cPLA2 to neurites...
  29. pmc Macrophage sub-populations and the lipoxin A4 receptor implicate active inflammation during equine tendon repair
    Stephanie Georgina Dakin
    Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hatfield, United Kingdom
    PLoS ONE 7:e32333. 2012
    ....
  30. ncbi request reprint Role of sugars in surface microbe-host interactions and immune reaction modulation
    David H Lloyd
    Department of Veterinary Clinical Sciences, Royal Veterinary College, Hawkshead Campus, North Mymms, Hertfordshire, UK
    Vet Dermatol 18:197-204. 2007
    ..More complete characterization of sugar receptors and their ligands will provide further keys to use of carbohydrates in immunomodulation and infection control in skin...
  31. doi request reprint LRRfinder2.0: a webserver for the prediction of leucine-rich repeats
    Victoria Offord
    Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, Hatfield, UK
    Innate Immun 19:398-402. 2013
    ..0 utilizes scoring matrices comprising more than 60,000 LRR motifs from more than 200 species. The underlying TLR database tLRRdb contains more than 3500 manually annotated sequences, augmenting identification of irregular LRR motifs. ..
  32. pmc TLR5 risk-associated haplotype for canine inflammatory bowel disease confers hyper-responsiveness to flagellin
    Aarti Kathrani
    Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hatfield, Hertfordshire, United Kingdom
    PLoS ONE 7:e30117. 2012
    ..Blocking the hyper-responsive receptor found in susceptible dogs with IBD may alleviate the inappropriate inflammation seen in this disease...
  33. ncbi request reprint A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cells
    Julien Guergnon
    Laboratoire de Signalisation Immunoparasitaire, URA CNRS 1960, Departement de Parasitologie, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Cell Microbiol 5:709-16. 2003
    ..Interestingly, both pharmacological inhibition of TNF synthesis and neutralizing anti-TNF antibodies lead to a reduction in proliferation and a 50% drop in NF-kappaB activation, without inducing apoptosis...
  34. ncbi request reprint Differential responses of bovine macrophages to infection with bovine-specific and non-bovine specific mycobacteria
    Jenny Piercy
    Institute for Animal Health, Compton, Berkshire, UK
    Tuberculosis (Edinb) 87:415-20. 2007
    ..Levels of nitric oxide and tumour necrosis factor production, markers of antimicrobial activity, were found to be associated with a specific mycobacterial strain, and varied between cell subsets...
  35. ncbi request reprint Cytokine responses of bovine dendritic cells and T cells following exposure to live or inactivated bovine respiratory syncytial virus
    Dirk Werling
    Institute for Animal Health, Compton, Berks, United Kingdom
    J Leukoc Biol 72:297-304. 2002
    ..It is possible, however, that differences in the pattern of cytokines produced by MoDC exposed to live or inactivated virus may influence the development of the primary CD4(+) T cell response in vivo...
  36. ncbi request reprint Peripheral blood-derived bovine dendritic cells promote IgG1-restricted B cell responses in vitro
    Anna A Bajer
    Department of Veterinary Pathobiology, Program for Prevention of Animal Infectious Diseases, University of Missouri, Columbia, MO 65211, USA
    J Leukoc Biol 73:100-6. 2003
    ..Collectively, these results suggest that direct B cell-DC interactions may promote an immunoglobulin-isotype expression pattern consistent with type 2 responses, independent of direct T cell involvement...
  37. ncbi request reprint Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes
    Martin Baumgartner
    Laboratoire de Signalisation Immunoparasitaire, Departement d Immunologie, Institut Pasteur, Paris, France
    Blood 101:1874-81. 2003
    ..We propose that Theileria-induced lymphocyte proliferation and permanent activation of Hck stems from down-regulation of PAG/Cbp and the concomitant constitutive loss of the negative regulator Csk from the GEMs of transformed B cells...
  38. pmc Expression of caveolin by bovine lymphocytes and antigen-presenting cells
    James Harris
    Division of Immunology and Pathology, Institute for Animal Health, Compton Laboratory, Near Newbury, Berkshire RG20 7NN, UK
    Immunology 105:190-5. 2002
    ..These data provide evidence for the presence of caveolin in bovine lymphocytes and antigen-presenting cells...
  39. ncbi request reprint c-Jun NH2-terminal kinase/c-Jun signaling promotes survival and metastasis of B lymphocytes transformed by Theileria
    Regina Lizundia
    Institut Cochin, Departement de Maladies Infectieuses, Paris, France
    Cancer Res 66:6105-10. 2006
    ..The similarity between Theileria-transformed B cells with human B lymphomas argues that exploiting the reversible nature of Theileria-induced transformation could throw light on the mechanisms underlying human malignancies...
  40. ncbi request reprint Lack of TNF alpha supports persistence of a plasmid encoding the bovine leukaemia virus in TNF(-/-) mice
    Catherine Muller
    Institute of Animal Sciences, ETH Zurich, Schornenstrasse 16, CH 8603 Schwerzenbach, Switzerland
    Vet Immunol Immunopathol 92:15-22. 2003
    ....
  41. ncbi request reprint Cross-reactivity of mAbs to human CD antigens with cells from cattle
    Paul Sopp
    Institute for Animal Health, Compton RG20 7NN, UK
    Vet Immunol Immunopathol 119:106-14. 2007
    ..mAb to human CD9, CD11a, CD14, CD18, CD21, CD23, CD29, CD44, CD45R, CD47, CD49d and CD172a cross-reacted with bovine cells and mAb to CD22, CD88, CD119 and CD163 stained CD antigens that have not previously been identified in cattle...