Research Topics
Genomes and Genes | Dirk WerlingSummaryAffiliation: Royal Veterinary College Country: UK Publications
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Publications
Differential production of cytokines, reactive oxygen and nitrogen by bovine macrophages and dendritic cells stimulated with Toll-like receptor agonistsDirk Werling
Institute of Veterinary Virology, University of Bern, CH 3012 Bern, Switzerland
Immunology 111:41-52. 2004..Collectively, our results point to profound differences in pathogen-derived signal-response coupling occurring commensurate with distinct functions carried out by Mphi or DC...- The bovine chemokine receptors and their mRNA abundance in mononuclear phagocytesStephanie Widdison
Institute for Animal Health, Compton, Newbury, Berkshire, UK
BMC Genomics 11:439. 2010..Although chemokine receptors have been well characterised in humans, the chemokine receptor repertoire of cattle is not well characterised and many sequences are yet to be experimentally validated... - Molecular evolution of bovine Toll-like receptor 2 suggests substitutions of functional relevanceOliver C Jann
The Roslin Institute and Royal Dick School of Veterinary Studies, University of Edinburgh, Roslin Biocentre, Midlothian, UK
BMC Evol Biol 8:288. 2008.... 
Innate immunity and vaccinationDirk Werling
Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
Vet Immunol Immunopathol 112:1. 2006- Ability to differentiate between cp and ncp BVDV by microarrays: towards an application in clinical veterinary medicine?Dirk Werling
Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
Vet Immunol Immunopathol 108:157-64. 2005..These data will help to further understand our knowledge regarding BVDV infection, and will especially help to understand differences in cellular responses to cpe and ncp biotypes... - Binding and entry of respiratory syncytial virus into host cells and initiation of the innate immune responseJames Harris
Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK
Cell Microbiol 5:671-80. 2003..This review summarizes our current understanding of RSV binding and uptake in mammalian cells and how these initial interactions influence the subsequent innate immune response generated... 
Variation matters: TLR structure and species-specific pathogen recognitionDirk Werling
Royal Veterinary College Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield, AL9 7TA, UK
Trends Immunol 30:124-30. 2009..Here, we aim to summarize our current knowledge of species-specific TLR responses and briefly discuss that vaccine efficacy in relevant host species might be improved by considering the species-specific TLR responses...- Pattern recognition receptors in companion and farm animals - the key to unlocking the door to animal disease?Dirk Werling
Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
Vet J 174:240-51. 2007..In this review, current knowledge regarding these receptors in species of veterinary relevance is summarised... - Expression of TOLL-like receptors (TLR) by bovine antigen-presenting cells-potential role in pathogen discrimination?Dirk Werling
Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
Vet Immunol Immunopathol 112:2-11. 2006.... - Inflamm-aging and arachadonic acid metabolite differences with stage of tendon diseaseStephanie Georgina Dakin
Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hatfield, United Kingdom
PLoS ONE 7:e48978. 2012.... - TOLL-like receptors linking innate and adaptive immune responseDirk Werling
Institute of Veterinary Virology, University of Berne, , CH-3012 Bern, Switzerland
Vet Immunol Immunopathol 91:1-12. 2003.... - Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon responseAmanda Gibson
The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, UK
Dev Comp Immunol 36:332-41. 2012.... - Host species-specific usage of the TLR4-LPS receptor complexRegina Lizundia
Royal Veterinary College, Hawkshead Campus, Hatfield, UK
Innate Immun 14:223-31. 2008..However, no clear role for the RSV F protein of either human or bovine RSV alone in stimulating TLR4-dependent NF-kappaB activation was observed... - Expression and function of Toll-like receptor 4 in the endometrial cells of the uterusShan Herath
Royal Veterinary College, Department of Veterinary Clinical Sciences, University of London, North Mymms, Hatfield, Hertfordshire AL9 7TA, United Kingdom
Endocrinology 147:562-70. 2006..In conclusion, epithelial and stromal cells detect and respond to bacteria, which modulate their endocrine function... - Caveolae and caveolin in immune cells: distribution and functionsJames Harris
Sir William Dunn School of Pathology, South Parks Road, OX1 3RE, Oxford, UK
Trends Immunol 23:158-64. 2002..Here, we review the distribution and role of caveolae and caveolin in mammalian immune cells... - Gene expression of selected signature cytokines of T cell subsets in duodenal tissues of dogs with and without inflammatory bowel diseaseSilke Schmitz
Royal Veterinary College, University of London, Department of Veterinary Clinical Sciences, Hawkshead Campus, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK
Vet Immunol Immunopathol 146:87-91. 2012..Thus, no clear evidence for the involvement of Th-17 signature cytokines in canine IBD at the mRNA level could be shown. The contribution of specific T cell subsets to the pathogenesis of canine IBD warrants further investigation... - Identification and functional characterization of a bovine orthologue to DC-SIGNYoshika Yamakawa
Department of Pathology and Infectious Diseases, Royal Veterinary College, Hatfield, UK
J Leukoc Biol 83:1396-403. 2008..Thus, there is evidence of a DC-SIGN-like molecule expressed specifically by bovine DC. This molecule may play an important role in the infection of bovine (DC) cells with M. bovis... - Polymorphisms in the TLR4 and TLR5 gene are significantly associated with inflammatory bowel disease in German shepherd dogsAarti Kathrani
Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hatfield, United Kingdom
PLoS ONE 5:e15740. 2010..Further studies are required to confirm the functional importance of these polymorphisms in the pathogenesis of this disease... - Evaluation of the effect of phosphodiesterase on equine platelet activation and the effect of antigen challenge on platelet phosphodiesterase activity in horses with recurrent airway obstructionBettina Dunkel
Department of Veterinary Clinical Sciences, The Royal Veterinary College, North Mymms, Herts AL9 7TA, England
Am J Vet Res 71:534-40. 2010.... - Assessment and quantification of post-weaning multi-systemic wasting syndrome severity at farm levelPablo Alarcon
Department of Veterinary Clinical Sciences, Royal Veterinary College, Hatfield AL9 7TA, United Kingdom
Prev Vet Med 98:19-28. 2011..5. The identified farm level PMWS severities will be used to identify risk factors related to these, to assess the efficacy of PCV2 vaccination and investigating the economic impact of potential control measures... - Potential evidence for biotype-specific chemokine profile following BVDV infection of bovine macrophagesStephen Burr
Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
Vet Immunol Immunopathol 150:123-7. 2012..This study helps to further our understanding of the pathogenesis of BVDV infection, highlighting biotype-specific cellular responses... - Yeast-surface expressed BVDV E2 protein induces a Th1/Th2 response in naïve T cellsRobert Patterson
Royal Veterinary College, Department of Pathology and Infectious Diseases, Hawkshead Lane, Hatfield AL9 7TA, UK
Dev Comp Immunol 37:107-14. 2012..Our studies demonstrate a use for S. cerevisiae as a vehicle for transporting BVDV vaccine antigen to antigen-presenting cell in order to elicit cell-mediated immunity even in naïve animals... - Toll-like receptors in domestic animalsThomas W Jungi
Institute of Veterinary Virology, Vetsuisse Faculty, University of Bern, Laenggasstrasse 122, CH 3001 Bern, Switzerland
Cell Tissue Res 343:107-20. 2011..In a few cases, expression of a given amino acid in the extracellular (ligand-binding) portion of TLR members could be associated with infectious diseases. This will be discussed below... - Inhibition of cytosolic Phospholipase A2 prevents prion peptide-induced neuronal damage and co-localisation with Beta III TubulinVictoria Last
Department of Pathology and Infectious Diseases, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, UK
BMC Neurosci 13:106. 2012..Here we report the effects of the prion peptide fragment HuPrP106-126 on the PLA2 cascade in primary cortical neurons and translocation of cPLA2 to neurites... - Comparison of TNFα responses induced by Toll-like receptor ligands and probiotic Enterococcus faecium in whole blood and peripheral blood mononuclear cells of healthy dogsSilke Schmitz
Royal Veterinary College London, Department of Veterinary Clinical Sciences, Hawkshead Lane, North Mymms, AL9 7TA, United Kingdom Electronic address
Vet Immunol Immunopathol 153:170-4. 2013..In conclusion, WB stimulation should be considered a valid alternative to PBMC stimulation in the canine system... - Expression of trefoil factor genes in the duodenum and colon of dogs with inflammatory bowel disease and healthy dogsSilke Schmitz
Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hawkshead Campus, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire, AL9 7TA, United Kingdom
Vet Immunol Immunopathol 151:168-72. 2013..Whether this is a cause or consequence of IBD could not be established... - Macrophage sub-populations and the lipoxin A4 receptor implicate active inflammation during equine tendon repairStephanie Georgina Dakin
Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hatfield, United Kingdom
PLoS ONE 7:e32333. 2012.... - Role of sugars in surface microbe-host interactions and immune reaction modulationDavid H Lloyd
Department of Veterinary Clinical Sciences, Royal Veterinary College, Hawkshead Campus, North Mymms, Hertfordshire, UK
Vet Dermatol 18:197-204. 2007..More complete characterization of sugar receptors and their ligands will provide further keys to use of carbohydrates in immunomodulation and infection control in skin... - TLR5 risk-associated haplotype for canine inflammatory bowel disease confers hyper-responsiveness to flagellinAarti Kathrani
Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, Hatfield, Hertfordshire, United Kingdom
PLoS ONE 7:e30117. 2012..Blocking the hyper-responsive receptor found in susceptible dogs with IBD may alleviate the inappropriate inflammation seen in this disease... - Peripheral blood-derived bovine dendritic cells promote IgG1-restricted B cell responses in vitroAnna A Bajer
Department of Veterinary Pathobiology, Program for Prevention of Animal Infectious Diseases, University of Missouri, Columbia, MO 65211, USA
J Leukoc Biol 73:100-6. 2003..Collectively, these results suggest that direct B cell-DC interactions may promote an immunoglobulin-isotype expression pattern consistent with type 2 responses, independent of direct T cell involvement... - Cytokine responses of bovine dendritic cells and T cells following exposure to live or inactivated bovine respiratory syncytial virusDirk Werling
Institute for Animal Health, Compton, Berks, United Kingdom
J Leukoc Biol 72:297-304. 2002..It is possible, however, that differences in the pattern of cytokines produced by MoDC exposed to live or inactivated virus may influence the development of the primary CD4(+) T cell response in vivo... - A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cellsJulien Guergnon
Laboratoire de Signalisation Immunoparasitaire, URA CNRS 1960, , Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
Cell Microbiol 5:709-16. 2003..Interestingly, both pharmacological inhibition of TNF synthesis and neutralizing anti-TNF antibodies lead to a reduction in proliferation and a 50% drop in NF-kappaB activation, without inducing apoptosis... - Differential responses of bovine macrophages to infection with bovine-specific and non-bovine specific mycobacteriaJenny Piercy
Institute for Animal Health, Compton, Berkshire, UK
Tuberculosis (Edinb) 87:415-20. 2007..Levels of nitric oxide and tumour necrosis factor production, markers of antimicrobial activity, were found to be associated with a specific mycobacterial strain, and varied between cell subsets... - c-Jun NH2-terminal kinase/c-Jun signaling promotes survival and metastasis of B lymphocytes transformed by TheileriaRegina Lizundia
Institut Cochin, , Paris, France
Cancer Res 66:6105-10. 2006..The similarity between Theileria-transformed B cells with human B lymphomas argues that exploiting the reversible nature of Theileria-induced transformation could throw light on the mechanisms underlying human malignancies... - Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytesMartin Baumgartner
Laboratoire de Signalisation Immunoparasitaire, , Institut Pasteur, Paris, France
Blood 101:1874-81. 2003..We propose that Theileria-induced lymphocyte proliferation and permanent activation of Hck stems from down-regulation of PAG/Cbp and the concomitant constitutive loss of the negative regulator Csk from the GEMs of transformed B cells... - Cross-reactivity of mAbs to human CD antigens with cells from cattlePaul Sopp
Institute for Animal Health, Compton RG20 7NN, UK
Vet Immunol Immunopathol 119:106-14. 2007..mAb to human CD9, CD11a, CD14, CD18, CD21, CD23, CD29, CD44, CD45R, CD47, CD49d and CD172a cross-reacted with bovine cells and mAb to CD22, CD88, CD119 and CD163 stained CD antigens that have not previously been identified in cattle... - Expression of caveolin by bovine lymphocytes and antigen-presenting cellsJames Harris
Division of Immunology and Pathology, Institute for Animal Health, Compton Laboratory, Near Newbury, Berkshire RG20 7NN, UK
Immunology 105:190-5. 2002..These data provide evidence for the presence of caveolin in bovine lymphocytes and antigen-presenting cells... - Lack of TNF alpha supports persistence of a plasmid encoding the bovine leukaemia virus in TNF(-/-) miceCatherine Muller
Institute of Animal Sciences, ETH Zurich, Schornenstrasse 16, CH 8603 Schwerzenbach, Switzerland
Vet Immunol Immunopathol 92:15-22. 2003....