A A Pitsillides

Summary

Affiliation: Royal Veterinary College
Country: UK

Publications

  1. pmc Reduced chondrogenic matrix accumulation by 4-methylumbelliferone reveals the potential for selective targeting of UDP-glucose dehydrogenase
    C E Clarkin
    Division of Diabetes and Nutritional Sciences, School of Medicine, Kings College London, University of London, United Kingdom
    Matrix Biol 30:163-8. 2011
  2. pmc Early effects of embryonic movement: 'a shot out of the dark'
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, Royal Veterinary College, London, UK
    J Anat 208:417-31. 2006
  3. doi request reprint A critical evaluation of specific aspects of joint development
    A A Pitsillides
    Department of Veterinary Basic Sciences, Royal Veterinary College, London, United Kingdom
    Dev Dyn 237:2284-94. 2008
  4. doi request reprint Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College Street, London, UK
    Methods Mol Biol 816:593-619. 2012
  5. doi request reprint Cartilage biology in osteoarthritis--lessons from developmental biology
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
    Nat Rev Rheumatol 7:654-63. 2011
  6. ncbi request reprint Identifying and characterizing the joint cavity-forming cell
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, Royal Veterinary College, London, UK
    Cell Biochem Funct 21:235-40. 2003
  7. doi request reprint The effect of estradiol on COX-2, EP2, and EP4 mRNA expression and the extracellular matrix in the cervix of the hypogonadotrophic, ovariectomized ewe
    C M Kershaw-Young
    Department of Veterinary Clinical Sciences, The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, United Kingdom
    Theriogenology 73:620-8. 2010
  8. ncbi request reprint Differential regulation of GDF-5 and FGF-2/4 by immobilisation in ovo exposes distinct roles in joint formation
    E Kavanagh
    Department of Veterinary Basic Sciences, The Royal Veterinary College, London, UK
    Dev Dyn 235:826-34. 2006
  9. pmc Defining boundaries during joint cavity formation: going out on a limb
    K J Lamb
    Department of Veterinary Basic Sciences, Royal Veterinary Collage, University of London, Royal College Street, London, NW1 OTU, UK
    Int J Exp Pathol 84:55-67. 2003
  10. pmc Cartilage to bone transitions in health and disease
    K A Staines
    Comparative Biomedical Sciences, The Royal Veterinary College, Royal College Street, London NW1 0TU, UK
    J Endocrinol 219:R1-R12. 2013

Collaborators

Detail Information

Publications35

  1. pmc Reduced chondrogenic matrix accumulation by 4-methylumbelliferone reveals the potential for selective targeting of UDP-glucose dehydrogenase
    C E Clarkin
    Division of Diabetes and Nutritional Sciences, School of Medicine, Kings College London, University of London, United Kingdom
    Matrix Biol 30:163-8. 2011
    ..Understanding how GAG synthesis is controlled and the mechanism of 4-MU action may inform its future clinical success...
  2. pmc Early effects of embryonic movement: 'a shot out of the dark'
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, Royal Veterinary College, London, UK
    J Anat 208:417-31. 2006
    ..Immobilization also exerts differential impact along the proximo-distal axis of the limb. Finally, our preliminary results support the possibility that embryonic hyperactivity influences the potential for postnatal muscle growth...
  3. doi request reprint A critical evaluation of specific aspects of joint development
    A A Pitsillides
    Department of Veterinary Basic Sciences, Royal Veterinary College, London, United Kingdom
    Dev Dyn 237:2284-94. 2008
    ..Here we review current theories on the mechanism by which these events are achieved in the joints of the developing limb...
  4. doi request reprint Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College Street, London, UK
    Methods Mol Biol 816:593-619. 2012
    ....
  5. doi request reprint Cartilage biology in osteoarthritis--lessons from developmental biology
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
    Nat Rev Rheumatol 7:654-63. 2011
    ..The latest studies are summarized and we discuss the concepts emerging from these findings in cartilage biology, in the light of our understanding of the developmental processes involved...
  6. ncbi request reprint Identifying and characterizing the joint cavity-forming cell
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, Royal Veterinary College, London, UK
    Cell Biochem Funct 21:235-40. 2003
    ..It is therefore imperative that we attempt to bridge the gap between the cell biology of such phenomena on the one hand, and the morphological approach to this same problem on the other...
  7. doi request reprint The effect of estradiol on COX-2, EP2, and EP4 mRNA expression and the extracellular matrix in the cervix of the hypogonadotrophic, ovariectomized ewe
    C M Kershaw-Young
    Department of Veterinary Clinical Sciences, The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, United Kingdom
    Theriogenology 73:620-8. 2010
    ..05). In conclusion, we inferred that estradiol regulates cervical COX-2 and EP(4) mRNA expression and may regulate cervical relaxation via the synthesis of prostaglandin E(2) and activation of the PGE(2) receptors EP(2) and EP(4)...
  8. ncbi request reprint Differential regulation of GDF-5 and FGF-2/4 by immobilisation in ovo exposes distinct roles in joint formation
    E Kavanagh
    Department of Veterinary Basic Sciences, The Royal Veterinary College, London, UK
    Dev Dyn 235:826-34. 2006
    ..These findings support a direct mechano-dependent role for FGF-2, but not FGF-4, in the cavitation process and indicate that GDF-5 is likely to influence chondrogenesis positively without contributing directly to joint cavity formation...
  9. pmc Defining boundaries during joint cavity formation: going out on a limb
    K J Lamb
    Department of Veterinary Basic Sciences, Royal Veterinary Collage, University of London, Royal College Street, London, NW1 OTU, UK
    Int J Exp Pathol 84:55-67. 2003
    ..In our opinion, studies that examine early patterning and also focus on local developmental alterations in tissue architecture are required in order to help elucidate the fundamental principals regulating joint formation...
  10. pmc Cartilage to bone transitions in health and disease
    K A Staines
    Comparative Biomedical Sciences, The Royal Veterinary College, Royal College Street, London NW1 0TU, UK
    J Endocrinol 219:R1-R12. 2013
    ....
  11. doi request reprint Cervical expression of hyaluronan synthases varies with the stage of the estrous cycle in the ewe
    K Perry
    Department of Veterinary Clinical Sciences, The Royal Veterinary College, University of London, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire, UK
    Theriogenology 77:1100-10. 2012
    ..These findings are in accordance with our previous results and explain the differences observed in the HA content and differing HA fragment size at different stages of the estrous cycle...
  12. pmc Giant cells in arthritic synovium
    L S Wilkinson
    Department of Rheumatology, Faculty of Clinical Sciences, University College, London, United Kingdom
    Ann Rheum Dis 52:182-4. 1993
    ..CONCLUSIONS: Giant cells in arthritic synovium appear to be of two types, one related to true synoviocytes and one to macrophages...
  13. pmc Mechanoadaptation of developing limbs: shaking a leg
    A S Pollard
    Comparative Biomedical Sciences, Royal Veterinary College, London, UK
    J Anat 224:615-23. 2014
    ....
  14. ncbi request reprint The mRNA expression of prostaglandin E receptors EP2 and EP4 and the changes in glycosaminoglycans in the sheep cervix during the estrous cycle
    C M Kershaw-Young
    Department of Veterinary Clinical Sciences, The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, United Kingdom
    Theriogenology 72:251-61. 2009
    ..We propose that prostaglandin E(2) binds to EP(2) and EP(4) stimulating hyaluronan synthesis, which may cause remodeling of the cervical extracellular matrix, culminating in cervical relaxation...
  15. doi request reprint Spontaneous osteoarthritis in Str/ort mice is unlikely due to greater vulnerability to mechanical trauma
    B Poulet
    Lifestyle Research Group, The Royal Veterinary College, Royal College Street, University of London, NW1 0TU, UK
    Osteoarthritis Cartilage 21:756-63. 2013
    ..We also develop finite element (FE) models to examine whether AC thickness may explain any differential vulnerability to load-induced lesions...
  16. ncbi request reprint Mechanical strain and fluid movement both activate extracellular regulated kinase (ERK) in osteoblast-like cells but via different signaling pathways
    H L Jessop
    Department of Veterinary Basic Sciences, The Royal Veterinary College, London, UK
    Bone 31:186-94. 2002
    ....
  17. doi request reprint Expression of endothelial nitric oxide synthase protein is not necessary for mechanical strain-induced nitric oxide production by cultured osteoblasts
    V Das-Gupta
    Department of Veterinary Basic Science, Royal Veterinary College, London, NW1 0TU, UK
    Osteoporos Int 23:2635-47. 2012
    ..We examined whether osteoblast endothelial NO synthase (eNOS)-derived NO production was regulated by HSP90. We found that HSP90 is essential for strain-related NO release but appears to be independent of eNOS in cultured osteoblasts...
  18. ncbi request reprint Acromial spur formation in patients with rotator cuff tears
    A F W Chambler
    Department of Orthopaedics, St Mary s Hospital, London, United Kingdom
    J Shoulder Elbow Surg 12:314-21. 2003
    ..The sites of oxytetracycline labeling appear to correlate with the sites of high ALP and G6PD activity, which supports the concept of spur formation being a secondary phenomenon in the presence of established rotator cuff tears...
  19. doi request reprint Characterizing a novel and adjustable noninvasive murine joint loading model
    Blandine Poulet
    Department of Veterinary Basic Sciences, The Royal Veterinary College, London, UK
    Arthritis Rheum 63:137-47. 2011
    ..The present study was undertaken to develop and characterize a novel nonsurgical murine model in which applied loads to the knee joint are highly adjustable...
  20. ncbi request reprint Methods for analyzing bone cell responses to mechanical loading using in vitro monolayer and organ culture models
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, The Royal Veterinary College, London, UK
    Methods Mol Med 80:399-422. 2003
  21. ncbi request reprint Influence of epitopes CD44v3 and CD44v6 in the invasive behavior of fibroblast-like synoviocytes derived from rheumatoid arthritic joints
    Auragun Wibulswas
    University College London, London, UK
    Arthritis Rheum 46:2059-64. 2002
    ..To investigate the functional implications of CD44 splice variant expression in fibroblast-like synoviocytes (FLS) obtained from patients with rheumatoid arthritis (RA)...
  22. ncbi request reprint Keratan sulfate epitopes exhibit a conserved distribution during joint development that remains undisclosed on the basis of glycosaminoglycan charge density
    Emma Kavanagh
    Department of Veterinary Basic Sciences, The Royal Veterinary College, London, United Kingdom
    J Histochem Cytochem 50:1039-47. 2002
    ..This is particularly evident for KS, which exhibits immunolabeling patterns in joints from different species that is consistent with a conserved functional role in chondrogenesis...
  23. ncbi request reprint Age-related changes in the response of human articular cartilage to IL-1alpha and transforming growth factor-beta (TGF-beta): chondrocytes exhibit a diminished sensitivity to TGF-beta
    Mark S Hickery
    Department of Cell and Molecular Biology, Section for Connective Tissue Research, BMC C12, 221 84, Lund, Sweden
    J Biol Chem 278:53063-71. 2003
    ..The antianabolic effects of IL-1alpha are, on the other hand, likely to be independent of any direct modification in UDPGD activity and manifest equally in human cartilage of all ages...
  24. doi request reprint Heterotypic contact reveals a COX-2-mediated suppression of osteoblast differentiation by endothelial cells: A negative modulatory role for prostanoids in VEGF-mediated cell: cell communication?
    Claire E Clarkin
    Department of Veterinary Basic Sciences, Royal Veterinary College, University of London, London, UK
    Exp Cell Res 314:3152-61. 2008
    ....
  25. ncbi request reprint Non-invasive axial loading of mouse tibiae increases cortical bone formation and modifies trabecular organization: a new model to study cortical and cancellous compartments in a single loaded element
    Roberto L de Souza
    Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
    Bone 37:810-8. 2005
    ....
  26. ncbi request reprint Sympathetic nervous system does not mediate the load-induced cortical new bone formation
    Roberto L de Souza
    Department of Veterinary Basic Sciences, Royal Veterinary College, London, United Kingdom
    J Bone Miner Res 20:2159-68. 2005
    ....
  27. ncbi request reprint Osteoblast-like cells from estrogen receptor alpha knockout mice have deficient responses to mechanical strain
    Helen L Jessop
    Department of Veterinary Basic Sciences, The Royal Veterinary College, London, United Kingdom
    J Bone Miner Res 19:938-46. 2004
    ..This proliferative response can be blocked by selective estrogen receptor modulators and increased by transfection of additional ERalpha...
  28. ncbi request reprint A specific mechanomodulatory role for p38 MAPK in embryonic joint articular surface cell MEK-ERK pathway regulation
    Jo C Lewthwaite
    Department of Veterinary Basic Sciences, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, United Kingdom
    J Biol Chem 281:11011-8. 2006
    ....
  29. pmc Diverse range of fixed positional deformities and bone growth restraint provoked by flaccid paralysis in embryonic chicks
    Katherine J Lamb
    Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College Street, London, UK
    Int J Exp Pathol 84:191-9. 2003
    ..Whilst in ovo avian development clearly differs from human, our findings may have implications for the fetus, premature and term neonate receiving such non-depolarizing neuromuscular blocking drugs...
  30. ncbi request reprint Osteocytes use estrogen receptor alpha to respond to strain but their ERalpha content is regulated by estrogen
    Gul Zaman
    Department of Basic Sciences, The Royal Veterinary College, University of London, London, United Kingdom
    J Bone Miner Res 21:1297-306. 2006
    ....
  31. ncbi request reprint Aging enhances a mechanically-induced reduction in tendon strength by an active process involving matrix metalloproteinase activity
    Jayesh Dudhia
    Department of Veterinary Clinical Sciences, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK
    Aging Cell 6:547-56. 2007
    ....
  32. ncbi request reprint Evaluation of VEGF-mediated signaling in primary human cells reveals a paracrine action for VEGF in osteoblast-mediated crosstalk to endothelial cells
    Claire E Clarkin
    Department of Veterinary Basic Sciences, Royal Veterinary College, University of London, London, UK
    J Cell Physiol 214:537-44. 2008
    ..These findings may offer novel regimes for modulating pathological bone remodeling anomalies through the control of the closely coupled vascular supply...
  33. ncbi request reprint Selective activation of the MEK-ERK pathway is regulated by mechanical stimuli in forming joints and promotes pericellular matrix formation
    Edward R Bastow
    Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College St, London, NW1 0TU, United Kingdom
    J Biol Chem 280:11749-58. 2005
    ....
  34. doi request reprint Heparan sulfate-dependent ERK activation contributes to the overexpression of fibrotic proteins and enhanced contraction by scleroderma fibroblasts
    Yunliang Chen
    University College London, London, UK
    Arthritis Rheum 58:577-85. 2008
    ....
  35. ncbi request reprint Human osteoblasts' proliferative responses to strain and 17beta-estradiol are mediated by the estrogen receptor and the receptor for insulin-like growth factor I
    Ming Zhao Cheng
    The Royal Veterinary College, University of London, United Kingdom
    J Bone Miner Res 17:593-602. 2002
    ..In both human cell types (unlike rat cells) the effects of strain and IGF-II as well as estradiol and IGF-I can be blocked at the IGF-I receptor...