Research Topics
| Alan StittSummaryAffiliation: Queen's University Belfast Country: UK Publications
| Collaborators
|
Detail Information
Publications
The 67-kd laminin receptor is preferentially expressed by proliferating retinal vessels in a murine model of ischemic retinopathyA W Stitt
Department of Ophthalmology, The Queen s University of Belfast, United Kingdom
Am J Pathol 152:1359-65. 1998..The differential expression of 67LR between proliferating and quiescent retinal vessels suggests that this laminin receptor is an important and novel target for future chemotherapeutic intervention during proliferative vasculopathies...
Advanced glycation endproduct modified basement membrane attenuates endothelin-1 induced [Ca2+]i signalling and contraction in retinal microvascular pericytesSarah-Jane Hughes
Centre of Vision Science, Institute of Clinical Sciences, The Royal Victoria Hospital, Belfast, Northern Ireland
Mol Vis 10:996-1004. 2004..AGE modification of vascular BMs may contribute to the retinal hemodynamic abnormalities observed during diabetes...- The maillard reaction in eye diseasesAlan W Stitt
Ophthalmic Research Centre, Queen s University Belfast, Royal Victoria Hospital, Grosvenor Road, Belfast, BT12 6BA, Northern Ireland
Ann N Y Acad Sci 1043:582-97. 2005..It also outlines recent pharmaceutical strategies to inhibit Maillard reaction products and provides links to how these may serve to limit ocular cell dysfunction... 
Advances in our understanding of diabetic retinopathyAlan W Stitt
Centre for Vision and Vascular Science, Queen s University of Belfast, The Royal Victoria Hospital, Belfast BT12 6BA, UK
Clin Sci (Lond) 125:1-17. 2013..We assess these findings and discuss how they could eventually lead to new therapeutic options for diabetic retinopathy...- Eyes open to stem cells: safety trial may pave the way for cell therapy to treat retinal disease in patientsReinhold J Medina
Centre for Vision and Vascular Science, School of Medicine, Dentistry and Biomedical Science, Queen s University Belfast, Royal Victoria Hospital, Belfast BT12 6BA, Northern Ireland, UK
Stem Cell Res Ther 2:47. 2011..While there are some hurdles to clear, the ACT trial is a fine example of translational research that could eventually pave the way for a range of stem cell therapies for the retina and other tissues... - Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identitiesReinhold J Medina
Centre for Vision and Vascular Science, School of Medicine, Dentistry and Biomedical Science, Queen s University Belfast, Belfast, BT12 6BA, UK
BMC Med Genomics 3:18. 2010..This study provides a detailed molecular fingerprint for two EPC subtypes: early EPCs (eEPCs) and outgrowth endothelial cells (OECs)... - Advanced glycation and retinal pathology during diabetesAlan W Stitt
Centre for Vision Science, Queen s University of Belfast, Royal Victoria Hospital, Grosvenor Road, Belfast, BT12 6BA, Northern Ireland, UK
Pharmacol Rep 57:156-68. 2005..It will also describe how ongoing pharmaceutical strategies to inhibit AGE formation and thereby attenuate their pathogenic influence during chronic hyperglycemia may play a significant role in the treatment of diabetic retinopathy... - Impaired retinal angiogenesis in diabetes: role of advanced glycation end products and galectin-3Alan W Stitt
Ophthalmic and Vision Science Research Centre, Queens University Belfast, Royal Victoria Hospital, Grosvenor Road, Belfast, BT12 6BA, Northern Ireland
Diabetes 54:785-94. 2005..The data suggest a significant suppression of angiogenesis by the retinal microvasculature during diabetes and implicate AGEs and AGE-receptor interactions in its causation... - Substrates modified by advanced glycation end-products cause dysfunction and death in retinal pericytes by reducing survival signals mediated by platelet-derived growth factorA W Stitt
Ophthalmic Research Centre, Ophthalmology and Vision Science, Queen s University Belfast, Royal Victoria Hospital, Belfast, Northern Ireland, UK
Diabetologia 47:1735-46. 2004..The current study used an in vitro model, whereby retinal pericytes were exposed to AGE-modified substrate and the mechanisms underlying pericyte death explored... - AGEs and diabetic retinopathyAlan W Stitt
Centre for Vision and Vascular Science, Queens University Belfast, Northern Ireland, UK
Invest Ophthalmol Vis Sci 51:4867-74. 2010.... - A new advanced glycation inhibitor, LR-90, prevents experimental diabetic retinopathy in ratsA Bhatwadekar
Centre for Vision Science, Queen s University Belfast, Royal Victoria Hospital, Grosvenor Road Belfast BT 126BA, Northern Ireland, UK
Br J Ophthalmol 92:545-7. 2008..Diabetic retinopathy is associated with accumulation of advanced glycation end products in the retinal microvasculature. LR-90 is an effective multistage inhibitor of advanced glycation with renoprotective and anti-inflammatory properties... - Vascular stem cells and ischaemic retinopathiesAlan W Stitt
Centre for Vision and Vascular Science, School of Medicine, Dentistry and Biomedical Science, Queen s University Belfast, Royal Victoria Hospital, Belfast BT12 6BA, Northern Ireland, UK
Prog Retin Eye Res 30:149-66. 2011..When some of the remaining questions surrounding the clinical use of EPCs are addressed, they may provide an exciting new therapeutic option for treating ischaemic retinopathies... - Characterization of the advanced glycation end-product receptor complex in human vascular endothelial cellsA W Stitt
Department of Opthalmology, Queen s University of Belfast, Royal Victoria Hospital, Belfast, BT12 6BA, Northern Ireland, United Kingdom
Biochem Biophys Res Commun 256:549-56. 1999..In response to AGE-modified molecules, this complex is subject to upregulation, while the AGE-R2 component also displays increased phosphorylation possibly leading to enhanced signal transduction... - Expression of vascular endothelial growth factor (VEGF) and its receptors is regulated in eyes with intra-ocular tumoursA W Stitt
Department of Ophthalmology, Queen s University of Belfast, Northern Ireland, U K
J Pathol 186:306-12. 1998..Retinal vascular endothelia may respond to high intra-ocular levels of VEGF by increasing expression of their VEGF receptors, a phenomenon which could have relevance to neoplasm-related ocular neovascularization... - Advanced glycation end-product receptor interactions on microvascular cells occur within caveolin-rich membrane domainsA W Stitt
Department of Ophthalmology, The Queen s University of Belfast, Northern Ireland, UK
FASEB J 14:2390-2. 2000..It is significant that the AGE-R complex localizes to these organelles, because this may have implications for AGE binding, internalization, signal transduction, and the modulation of AGE-receptor-mediated vascular cell dysfunction... - The AGE inhibitor pyridoxamine inhibits development of retinopathy in experimental diabetesAlan Stitt
Department of Ophthalmology, Institute of Clinical Science, The Royal Victoria Hospital, Queen s University of Belfast, Belfast, Northern Ireland
Diabetes 51:2826-32. 2002..These results indicate that the AGE/ALE inhibitor PM protected against a range of pathological changes in the diabetic retina and may be useful for treating diabetic retinopathy... - Ocular wounding prevents pre-retinal neovascularization and upregulates PEDF expression in the inner retinaAlan W Stitt
Ophthalmic Research Centre, Queen s University Belfast, Institute of Clinical Sciences, Royal Victoria Hospital, Belfast, Northern Ireland, UK
Mol Vis 10:432-8. 2004.... - Role of vascular endothelial growth factor and placental growth factors during retinal vascular development and hyaloid regressionSusan A Feeney
Department of Ophthalmology, The Queen s University of Belfast, The Royal Victoria Hospital, Belfast, Northern Ireland, United Kingdom
Invest Ophthalmol Vis Sci 44:839-47. 2003..This study has sought to elucidate the expression patterns and modulatory role of these growth factors during retinal vascular development and hyaloid regression in the neonatal mouse... - Retinopathy is reduced during experimental diabetes in a mouse model of outer retinal degenerationTanyth E de Gooyer
Centre for Vision Science, Queens University Belfast, Institute of Clinical Science Block A, Royal Victoria Hospital, Belfast, Northern Ireland
Invest Ophthalmol Vis Sci 47:5561-8. 2006..The study was designed to investigate whether degeneration of the outer retina has any impact on hypoxia, to examine diabetes-related retinal gene expression responses, and to assess lesions of diabetic retinopathy... - Recombinant alpha2(IV)NC1 domain of type IV collagen is an effective regulator of retinal capillary endothelial cell proliferation and inhibits pre-retinal neovascularisationGary Coleman
Centre for Vision Science, School of Biomedical Science, Queen s University Belfast, Royal Victoria Hospital, Grosvenor Road, Belfast, BT12 6BA, Northern Ireland, UK
Graefes Arch Clin Exp Ophthalmol 245:581-7. 2007..In the current investigation we examined the potential for alpha2(IV)NC1 to regulate retinal microvascular endothelial cell function using a range of in vitro and in vivo assay systems... - Retinal endothelial cell apoptosis stimulates recruitment of endothelial progenitor cellsAshay D Bhatwadekar
Centre for Vision Science, Queen s University Belfast, Royal Victoria Hospital, Belfast, Northern Ireland, United Kingdom
Invest Ophthalmol Vis Sci 50:4967-73. 2009..This study was conducted to determine how the presence of apoptotic bodies at sites of endothelial damage may influence participation of EPCs in retinal microvascular repair... - Inhibition of advanced glycation and absence of galectin-3 prevent blood-retinal barrier dysfunction during short-term diabetesPaul Canning
Centre for Vision Science, Queen s University Belfast, Royal Victoria Hospital, Grosvenor Road, Belfast BT12 6BA, Northern Ireland, UK
Exp Diabetes Res 2007:51837. 2007..Prevention of AGE formation or genetic deletion of Gal-3 can effectively prevent these acute diabetic retinopathy changes... - Inhibition of tumor necrosis factor-alpha improves physiological angiogenesis and reduces pathological neovascularization in ischemic retinopathyTom A Gardiner
Ophthalmology and Vision Science, Queen s University Belfast, Royal Victoria Hospital, Grosvenor Road, Belfast BT12 6BA, Northern Ireland, UK
Am J Pathol 166:637-44. 2005..HP provides a useful tool for mapping and quantifying tissue hypoxia in experimental ischemic retinopathy... - Outgrowth endothelial cells: characterization and their potential for reversing ischemic retinopathyReinhold J Medina
Centre for Vision and Vascular Science, School of Medicine, Dentistry and Biomedical Science, Queen s University Belfast, Belfast, United Kingdom
Invest Ophthalmol Vis Sci 51:5906-13. 2010.... - The role of advanced glycation end products in retinal microvascular leukostasisTara C B Moore
Department of Ophthalmology, Royal Victoria Hospital, The Queen s University of Belfast, Northern Ireland
Invest Ophthalmol Vis Sci 44:4457-64. 2003.... - Rod photoreceptor loss in Rho-/- mice reduces retinal hypoxia and hypoxia-regulated gene expressionTanyth E de Gooyer
Centre for Vision Science, Queen s University Belfast, Royal Victoria Hospital, Belfast, Northern Ireland, United Kingdom
Invest Ophthalmol Vis Sci 47:5553-60. 2006..This study was conducted to evaluate whether regions of the retinal neuropile become hypoxic during periods of high oxygen consumption and whether depletion of the outer retina reduces hypoxia and related changes in gene expression... - Differential modulation of angiogenesis by erythropoiesis-stimulating agents in a mouse model of ischaemic retinopathyCarmel M McVicar
Centre for Vision and Vascular Science, Queen s University Belfast, Belfast, Northern Ireland, United Kingdom
PLoS ONE 5:e11870. 2010..In the current study we have assessed the angiogenic potential of three ESAs; epoetin delta, darbepoetin alfa and epoetin beta using in vitro and in vivo models... - Advanced glycation end product (AGE) accumulation on Bruch's membrane: links to age-related RPE dysfunctionJosephine V Glenn
Centre for Vision and Vascular Science, School of Medicine, Dentistry and Biomedical Science, Queen s University, Northern Ireland, United Kingdom
Invest Ophthalmol Vis Sci 50:441-51. 2009..In the present study the impact of AGEs at the RPE-Bruch's membrane interface was evaluated, to establish how these modifications may contribute to age-related disease... - Advanced glycation and advanced lipoxidation: possible role in initiation and progression of diabetic retinopathyAlan W Stitt
Ophthalmic Research Centre, Queen s University of Belfast, BT12 6BA Northern Ireland, UK
Curr Pharm Des 10:3349-60. 2004..It will also outline how recent pharmaceutical strategies to inhibit AGE/ALE formation or limit their pathogenic influence during chronic hyperglycaemia may play a significant role in the treatment of diabetic retinopathy... - Effects of an endothelin receptor antagonist on a model of hypertensive retinopathyLouise McDonald
Centre for Vision and Vascular Science, Queen s University Belfast, Belfast, Northern Ireland
Ophthalmic Res 43:99-107. 2010..ET-mediated vasoregulation abnormalities in the retinal microvasculature could play an associative role in lesion formation during hypertensive retinopathy... - The role of advanced glycation end products in retinal ageing and diseaseJosephine V Glenn
Centre for Vision and Vascular Science, School of Medicine, Dentistry and Biomedical Sciences, Queen s University Belfast, Belfast, BT12 6BA, Northern Ireland, UK
Biochim Biophys Acta 1790:1109-16. 2009..The therapeutic potential for novel agents that can ameliorate AGE formation of attenuate RAGE signalling in the retina is also discussed... - Upregulation of oxidative stress markers in human microvascular endothelial cells by complexes of serum albumin and digestion products of glycated caseinPermal Deo
Human Nutrition and Health Group, School of Biological Sciences, Queen s University Belfast, Belfast, Northern Ireland
J Biochem Mol Toxicol 23:364-72. 2009..In conclusion, in an in vitro cell system, digested dietary AGEs complexed with serum albumin play a role in the regulation of RAGE and downstream inflammatory pathways. AGE-R3 may protect against these effects... - Advanced glycation as a basis for understanding retinal aging and noninvasive risk predictionAnna M Pawlak
Centre for Vision Science, School of Biomedical Science, Queen s University Belfast, Belfast, UK
Ann N Y Acad Sci 1126:59-65. 2008.... - Proteomic profiling of the retinal dysplasia and degeneration chick retinaSorcha Finnegan
Centre for Vision Sciences, Institute of Clinical Science, Queen s University of Belfast, Belfast, Northern Ireland, UK
Mol Vis 16:7-17. 2010..The pathology displayed by the rdd chick resembles that observed in some of the more severe forms of human retinitis pigmentosa... - The role of advanced glycation in the pathogenesis of diabetic retinopathyAlan W Stitt
Department of Ophthalmology, Queen's University of Belfast, Royal Victoria Hospital, Belfast BT12 6BA, Northern Ireland, UK
Exp Mol Pathol 75:95-108. 2003..It will also outline how recent strategies to inhibit AGE formation or limit their pathogenic influence during chronic diabetes may have an important role to play in the treatment of retinopathy... - Advanced glycation end products and diabetic complicationsAlan W Stitt
Dept of Ophthalmology, Royal Victoria Hospital, Queen's University of Belfast, Northern Ireland
Expert Opin Investig Drugs 11:1205-23. 2002.... - Synthetic peptides interacting with the 67-kd laminin receptor can reduce retinal ischemia and inhibit hypoxia-induced retinal neovascularizationDorota Gebarowska
Centre of Ophthalmology and Vision Science and the School of Biology and Biochemistry, The Queen's University of Belfast, Royal Victoria Hospital, Belfast, Northern Ireland, United Kingdom
Am J Pathol 160:307-13. 2002..Agonism of this receptor may be valuable in reducing the hypoxia-stimulated release of angiogenic growth factors which drives retinal angiogenesis... - The pleiotropic effects of simvastatin on retinal microvascular endothelium has important implications for ischaemic retinopathiesReinhold J Medina
Centre for Vision Science, Queen s University Belfast, Belfast, United Kingdom
PLoS ONE 3:e2584. 2008..The objective of this study was to investigate the effects of clinically relevant concentrations of simvastatin on retinal endothelium in vitro and in vivo... - Advanced glycation of fibronectin impairs vascular repair by endothelial progenitor cells: implications for vasodegeneration in diabetic retinopathyAshay D Bhatwadekar
Centre for Vision Science, Queen s University Belfast, Royal Victoria Hospital, Northern Ireland, UK
Invest Ophthalmol Vis Sci 49:1232-41. 2008..The hypothesis for the study was that progressive basement membrane (BM) modification by advanced glycation end products (AGEs) contributes to impairment of EPC reparative function after diabetes-related endothelial injury... - Sources of PDGF expression in murine retina and the effect of short-term diabetesOrla T Cox
Ophthalmology Vision Science, Queen s University of Belfast, Institute of Clinical Science, Royal Victoria Hospital Belfast, Northern Ireland
Mol Vis 9:665-72. 2003..The retinal cell source(s) of PDGF remain unknown. It is important to understand how diabetes alters expression of this important growth factor... - Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitusCon Mallidis
Department of Obstetrics and Gynaecology, Institute of Clinical Sciences, School of Medicine, Queen s University of Belfast, Grosvenor Road, Belfast, UK
Hum Reprod 22:2169-77. 2007..As the receptor for AGEs (RAGE) is important to oxidative stress and cell dysfunction, we hypothesise, that it may be involved in sperm nDNA damage... - Confocal Raman microscopy can quantify advanced glycation end product (AGE) modifications in Bruch's membrane leading to accurate, nondestructive prediction of ocular agingJosephine V Glenn
Centre for Vision Science, School of Biomedical Science, Faculty of Medicine and Health Sciences, Queen s University Belfast, Belfast BT12 6BA, Northern Ireland
FASEB J 21:3542-52. 2007..000001). With further development, this Raman-based approach has the potential for noninvasive examination of AGE adducts in living eyes and ultimately to assess their precise pathogenic role in age-related diseases... - Advanced glycation of the Arg-Gly-Asp (RGD) tripeptide motif modulates retinal microvascular endothelial cell dysfunctionDenise M McDonald
Centre for Vision and Vascular Science, Queen s University Belfast, Royal Victoria Hospital, Grosvenor Road, Belfast, Northern Ireland, UK
Mol Vis 15:1509-20. 2009.... - Arteriolar involvement in the microvascular lesions of diabetic retinopathy: implications for pathogenesisTom A Gardiner
Centre for Vision Science, The Queen s University of Belfast, The Royal Victoria Hospital, Institute of Clinical Science, Belfast, Northern Ireland
Microcirculation 14:25-38. 2007..In particular, an emphasis will be placed on the relatively unappreciated role of arteriolar dysfunction in the clinical manifestations and pathology of this disease... - Intervention with an erythropoietin-derived peptide protects against neuroglial and vascular degeneration during diabetic retinopathyCarmel M McVicar
Centre for Vision and Vascular Science, Queen s University Belfast, Belfast, Northern Ireland, UK
Diabetes 60:2995-3005. 2011..A peptide based on the EPO helix-B domain (helix B-surface peptide [pHBSP]) is nonerythrogenic but retains tissue-protective properties, and this study evaluates its therapeutic potential in diabetic retinopathy... - Multiplex analysis of age-related protein and lipid modifications in human Bruch's membraneJ Renwick Beattie
Centre for Vision and Vascular Science, School of Medicine and Dentistry, Queen s University Belfast, Belfast, UK
FASEB J 24:4816-24. 2010..We present the first directly recorded evidence of the key role of heme in AGE/ALE formation... - Homodimerization is essential for the receptor for advanced glycation end products (RAGE)-mediated signal transductionHongliang Zong
Centre for Vision and Vascular Science, Queen s University Belfast, Royal Victoria Hospital, Belfast BT12 6BA, Northern Ireland, United Kingdom
J Biol Chem 285:23137-46. 2010..Thus, the data indicate that dimerization of RAGE represents an important component of RAGE-mediated cell signaling... - Inhibition of platelet-derived growth factor promotes pericyte loss and angiogenesis in ischemic retinopathyJennifer L Wilkinson-Berka
Department of Physiology, University of Melbourne, Parkville, Victoria, Australia
Am J Pathol 164:1263-73. 2004..In conclusion, PDGF is required for pericyte viability and the subsequent prevention of VEGF/VEGFR-2 overexpression and angiogenesis in ROP... - Involvement of MAPKs in endostatin-mediated regulation of blood-retinal barrier functionMatthew Campbell
UCD School of Biomolecular Biomedical Sciences, Conway Institute, University College Dublin, Dublin, Ireland
Curr Eye Res 31:1033-45. 2006..In order to determine the mechanism of endostatin action on BRB integrity, we have examined the effects of endostatin on a number of intracellular pathways implicated in endothelial cell physiology...