I R Grant

Summary

Affiliation: Queen's University Belfast
Country: UK

Publications

  1. ncbi request reprint Effect of chemical decontamination and refrigerated storage on the isolation of Mycobacterium avium subsp. paratuberculosis from heat-treated milk
    I R Grant
    Department of Food Science Food Microbiology, Queen s University of Belfast, Belfast, UK
    Lett Appl Microbiol 38:283-8. 2004
  2. pmc Phage display-derived binders able to distinguish Listeria monocytogenes from other Listeria species
    Josephine Morton
    Institute for Global Food Security, School of Biological Sciences, Queen s University, Belfast, Northern Ireland, United Kingdom
    PLoS ONE 8:e74312. 2013
  3. pmc Improved detection of Mycobacterium bovis infection in bovine lymph node tissue using immunomagnetic separation (IMS)-based methods
    Linda D Stewart
    Institute for Global Food Security, School of Biological Sciences, Queen s University Belfast, Belfast, Northern Ireland, United Kingdom
    PLoS ONE 8:e58374. 2013
  4. pmc Production and evaluation of antibodies and phage display-derived peptide ligands for immunomagnetic separation of Mycobacterium bovis
    Linda D Stewart
    School of Biological Sciences, Queen s University Belfast, Medical Biology Centre, Belfast, Northern Ireland, United Kingdom
    J Clin Microbiol 50:1598-605. 2012
  5. pmc Interaction between Mycobacterium avium subsp. paratuberculosis and environmental protozoa
    Lynne Whan
    Department of Food Science, Queen s University Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK
    BMC Microbiol 6:63. 2006
  6. ncbi request reprint Effect of higher pasteurization temperatures, and longer holding times at 72 degrees C, on the inactivation of Mycobacterium paratuberculosis in milk
    I R Grant
    Department of Food Science Food Microbiology, Queen s University of Belfast, UK
    Lett Appl Microbiol 28:461-5. 1999
  7. ncbi request reprint Comparative evaluation of the MGIT and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk
    I R Grant
    Department of Food Microbiology, Queen s University of Belfast, Belfast, UK
    J Appl Microbiol 95:196-201. 2003
  8. ncbi request reprint Zoonotic potential of Mycobacterium avium ssp. paratuberculosis: the current position
    I R Grant
    Department of Food Microbiology, Queen s University of Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK
    J Appl Microbiol 98:1282-93. 2005
  9. pmc Efficacy of various pasteurization time-temperature conditions in combination with homogenization on inactivation of Mycobacterium avium subsp. paratuberculosis in milk
    Irene R Grant
    Department of Food Microbiology, Queen s University of Belfast, Agriculture and Food Science Centre, Newforge Lane, Belfast BT9 5PX, United Kingdom
    Appl Environ Microbiol 71:2853-61. 2005
  10. ncbi request reprint Effect of high-temperature, short-time (HTST) pasteurization on milk containing low numbers of Mycobacterium paratuberculosis
    I R Grant
    Department of Food Science Food Microbiology, Queen s University of Belfast, Northern Ireland, UK
    Lett Appl Microbiol 26:166-70. 1998

Collaborators

  • M T Rowe
  • Leonid A Kulakov
  • M F Patterson
  • Stella Y Y Wong
  • M Friedman
  • Chen Situ
  • Lynne Whan
  • Antonio Foddai
  • Linda D Stewart
  • Christopher T Elliott
  • L D Stewart
  • Josephine Morton
  • Lyanne McCallan
  • James McNair
  • Leslie C Altic
  • Ciara E O'Reilly
  • Sangeeta Khare
  • A Foddai
  • C T Elliott
  • Ratthaphol Charlermroj
  • Alan Gordon
  • Nitsara Karoonuthaisiri
  • Suzan Thompson
  • L Dundee
  • Samuel Strain
  • Robert H Whitlock
  • Pat O'Mahony
  • Renato L Santos
  • Thomas A Ficht
  • L Garry Adams
  • Melissa Libal
  • Juan Romano
  • Lisa O'Connor
  • Wayne Anderson
  • Allison R Ficht
  • Shuping Zhang
  • John Donaghy
  • David Hunter
  • Peter Harvey
  • H J Ball

Detail Information

Publications30

  1. ncbi request reprint Effect of chemical decontamination and refrigerated storage on the isolation of Mycobacterium avium subsp. paratuberculosis from heat-treated milk
    I R Grant
    Department of Food Science Food Microbiology, Queen s University of Belfast, Belfast, UK
    Lett Appl Microbiol 38:283-8. 2004
    ..To assess the impact of chemical decontamination and refrigerated storage before culture on the recovery of Mycobacterium avium subsp. paratuberculosis from heat-treated milk...
  2. pmc Phage display-derived binders able to distinguish Listeria monocytogenes from other Listeria species
    Josephine Morton
    Institute for Global Food Security, School of Biological Sciences, Queen s University, Belfast, Northern Ireland, United Kingdom
    PLoS ONE 8:e74312. 2013
    ..L. monocytogenes-specific binders were obtained which could have potential application in novel detection tests for L. monocytogenes, benefiting both the food and medical industries. ..
  3. pmc Improved detection of Mycobacterium bovis infection in bovine lymph node tissue using immunomagnetic separation (IMS)-based methods
    Linda D Stewart
    Institute for Global Food Security, School of Biological Sciences, Queen s University Belfast, Belfast, Northern Ireland, United Kingdom
    PLoS ONE 8:e58374. 2013
    ..bovis infection in cattle. Adoption of the IMS-based methods for lymph node testing would have the potential to improve M. bovis detection in clinical samples...
  4. pmc Production and evaluation of antibodies and phage display-derived peptide ligands for immunomagnetic separation of Mycobacterium bovis
    Linda D Stewart
    School of Biological Sciences, Queen s University Belfast, Medical Biology Centre, Belfast, Northern Ireland, United Kingdom
    J Clin Microbiol 50:1598-605. 2012
    ..7 CFU/ml of lymph node tissue homogenate for IMS-PCR and IMS-culture, respectively). The optimized IMS method therefore has the potential to improve isolation of M. bovis from lymph nodes and hence the diagnosis of bovine tuberculosis...
  5. pmc Interaction between Mycobacterium avium subsp. paratuberculosis and environmental protozoa
    Lynne Whan
    Department of Food Science, Queen s University Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK
    BMC Microbiol 6:63. 2006
    ..The potential impact of ingestion of Map by two naturally occurring Acanthamoeba spp. on this pathogen's survival and chlorine resistance was investigated...
  6. ncbi request reprint Effect of higher pasteurization temperatures, and longer holding times at 72 degrees C, on the inactivation of Mycobacterium paratuberculosis in milk
    I R Grant
    Department of Food Science Food Microbiology, Queen s University of Belfast, UK
    Lett Appl Microbiol 28:461-5. 1999
    ..It was concluded that a longer holding time is more likely to achieve the complete inactivation of Myco. paratuberculosis in milk than a higher pasteurization temperature...
  7. ncbi request reprint Comparative evaluation of the MGIT and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk
    I R Grant
    Department of Food Microbiology, Queen s University of Belfast, Belfast, UK
    J Appl Microbiol 95:196-201. 2003
    ..paratuberculosis from milk...
  8. ncbi request reprint Zoonotic potential of Mycobacterium avium ssp. paratuberculosis: the current position
    I R Grant
    Department of Food Microbiology, Queen s University of Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK
    J Appl Microbiol 98:1282-93. 2005
  9. pmc Efficacy of various pasteurization time-temperature conditions in combination with homogenization on inactivation of Mycobacterium avium subsp. paratuberculosis in milk
    Irene R Grant
    Department of Food Microbiology, Queen s University of Belfast, Agriculture and Food Science Centre, Newforge Lane, Belfast BT9 5PX, United Kingdom
    Appl Environ Microbiol 71:2853-61. 2005
    ..avium subsp. paratuberculosis cells would have been present as predominantly declumped cells, which may possibly explain the greater inactivation achieved by the combination of pasteurization and homogenization...
  10. ncbi request reprint Effect of high-temperature, short-time (HTST) pasteurization on milk containing low numbers of Mycobacterium paratuberculosis
    I R Grant
    Department of Food Science Food Microbiology, Queen s University of Belfast, Northern Ireland, UK
    Lett Appl Microbiol 26:166-70. 1998
    ..IS900-based PCR confirmed that these acid-fast survivors were Myco. paratuberculosis. No viable Myco. paratuberculosis were isolated from HTST-pasteurized milk initially containing either 10 cfu ml-1 or 10 cfu 50 ml-1...
  11. pmc Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation
    I R Grant
    Department of Food Science Food Microbiology, The Queen s University of Belfast, Belfast BT9 5PX, United Kingdom
    Appl Environ Microbiol 64:3153-8. 1998
    ..The potential value of our IMS method is as an aid for rapid detection of M. paratuberculosis in milk when it is used in conjunction with end point detection methods, such as IS900 PCR or an enzyme-linked immunosorbent assay...
  12. ncbi request reprint Improved detection of Mycobacterium avium subsp. paratuberculosis In milk by immunomagnetic PCR
    I R Grant
    Department of Food Science Food Microbiology, The Queen s University of Belfast, Newforge Lane, Belfast, UK
    Vet Microbiol 77:369-78. 2000
    ..5% of the same 40 milk samples (sensitivity 23%, specificity 100%). IMS-PCR was also shown to be capable of detecting natural M. ptb infection in raw sheep's milk, and raw and commercially pasteurised cows' milk...
  13. ncbi request reprint Development of an IMS-PCR assay for the detection of Mycobacterium avium ssp. paratuberculosis in water
    L Whan
    Food Microbiology, The Queen s University of Belfast, Belfast, UK
    Lett Appl Microbiol 40:269-73. 2005
    ..To develop a sensitive detection method for Mycobacterium avium ssp. paratuberculosis (Map) in water by modifying and optimizing an existing immunomagnetic separation polymerase chain reaction (IMS-PCR) technique...
  14. ncbi request reprint Incidence of Mycobacterium paratuberculosis in raw sheep and goats' milk in England, Wales and Northern Ireland
    I R Grant
    Department of Food Science Microbiology, The Queen s University of Belfast, Newforge Lane, Belfast BT9 5PX, UK
    Vet Microbiol 79:123-31. 2001
    ..paratuberculosis were isolated by culture. The results of this study suggest that bulk raw sheep and goats' milk from these regions of the UK may not represent significant vehicles of transmission of M. paratuberculosis to humans...
  15. ncbi request reprint Comparative evaluation of four decontamination protocols for the isolation of Mycobacterium avium subsp. paratuberculosis from milk
    L Dundee
    Department of Food Science (Food Microbiology, Queen's University of Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK
    Lett Appl Microbiol 33:173-7. 2001
    ..The "best" decontamination protocol only recovered a fraction of the M.a. paratuberculosis cells present in a milk sample...
  16. ncbi request reprint Bactericidal effect of chlorine on Mycobacterium paratuberculosis in drinking water
    L B Whan
    Department of Food Science (Food Microbiology, Queen's University Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK
    Lett Appl Microbiol 33:227-31. 2001
    ..SIGNIFICANCE AND IMPACT OF THE STUDY: This work highlights the need for further research into the survival of M. paratuberculosis during water treatment...
  17. pmc Antibacterial activities of naturally occurring compounds against Mycobacterium avium subsp. paratuberculosis
    Stella Y Y Wong
    Institute of Agri food and Land Use, School of Biological Sciences, Queen s University Belfast, David Keir Building, Stranmillis Road, Belfast BT9 5AG, United Kingdom
    Appl Environ Microbiol 74:5986-90. 2008
    ..No difference in compound activity was observed between the three M. avium subsp. paratuberculosis strains studied. Possible mechanisms of the antimicrobial effects are discussed...
  18. pmc Effect of commercial-scale high-temperature, short-time pasteurization on the viability of Mycobacterium paratuberculosis in naturally infected cows' milk
    Irene R Grant
    Department of Food Science Food Microbiology, Queen s University of Belfast, Newforge Lane, Belfast BT9 5PX, N Ireland, UK
    Appl Environ Microbiol 68:602-7. 2002
    ..This study provides clear evidence that M. paratuberculosis bacteria in naturally infected milk are capable of surviving commercial high-temperature, short-time pasteurization if they are present in raw milk in sufficient numbers...
  19. ncbi request reprint Mycobacterium paratuberculosis and milk
    I R Grant
    Department of Food Science Microbiology, Queen s University, Belfast, N Ireland, UK
    Acta Vet Scand 44:261-6. 2003
    ..Hence, both raw and pasteurised cows' milk are potential vehicles of transmission of M. paratuberculosis to humans...
  20. pmc UV light inactivation of Mycobacterium avium subsp. paratuberculosis in milk as assessed by FASTPlaqueTB phage assay and culture
    Leslie C Altic
    Institute of Agri food and Land Use, School of Biological Sciences, Queen s University Belfast, UK
    Appl Environ Microbiol 73:3728-33. 2007
    ..avium subsp. paratuberculosis derived using the phage assay and culture results were not significantly different (P = 0.077)...
  21. ncbi request reprint Mycobacterium avium ssp. paratuberculosis and its potential survival tactics
    M T Rowe
    Food Microbiology Branch, Agriculture, Food and Environmental Science Division, Department of Agriculture and Rural Development for Northern Ireland, Belfast, UK
    Lett Appl Microbiol 42:305-11. 2006
    ..It then focuses on the potential mechanisms Map might employ to survive and disseminate in the environment, including interaction with protozoa and insects, dormancy, biofilm formation and aerosolization...
  22. ncbi request reprint A numerical taxonomic study of lactic acid bacteria isolated from irradiated pork and chicken packaged under various gas atmospheres
    I R Grant
    Faculty of Agriculture and Food Science, Queen s University of Belfast, UK
    J Appl Bacteriol 70:302-7. 1991
    ..Clusters 5 and 6 contained four pork and two chicken strains respectively and no type strains. Four test strains remained unclustered as did the other reference strains included in the study...
  23. doi request reprint Development of a novel phage-mediated immunoassay for the rapid detection of viable Mycobacterium avium subsp. paratuberculosis
    L D Stewart
    Institute for Global Food Security, School of Biological Sciences, Queen s University Belfast, Belfast, Northern Ireland, UK
    J Appl Microbiol 115:808-17. 2013
    ..The objective of this study was to develop a novel screening method for detection of viable Mycobacterium avium subsp. paratuberculosis (Map) in milk and faeces, as a rapid alternative to Map culture...
  24. pmc Incidence of Mycobacterium paratuberculosis in bulk raw and commercially pasteurized cows' milk from approved dairy processing establishments in the United Kingdom
    Irene R Grant
    Department of Food Science, Food Microbiology, Queen s University of Belfast, Northern Ireland, United Kingdom
    Appl Environ Microbiol 68:2428-35. 2002
    ..It was concluded that viable M. paratuberculosis is occasionally present at low levels in commercially pasteurized cows' milk in the United Kingdom...
  25. pmc Rapid assessment of the viability of Mycobacterium avium subsp. paratuberculosis cells after heat treatment, using an optimized phage amplification assay
    Antonio Foddai
    Institute of Agri food and Land Use, School of Biological Sciences, Queen s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, United Kingdom
    Appl Environ Microbiol 76:1777-82. 2010
    ..avium subsp. paratuberculosis-spiked samples...
  26. pmc Occurrence of Mycobacterium avium subsp. paratuberculosis in untreated water in Northern Ireland
    Lynne Whan
    Department of Food Microbiology, Queen s University of Belfast, Belfast BT9 5PX, Northern Ireland, United Kingdom
    Appl Environ Microbiol 71:7107-12. 2005
    ..This work indicates the need to determine the efficacy of water treatment processes to either kill or remove M. avium subsp. paratuberculosis from untreated water and the possible risks posed by contact with recreational water sources...
  27. pmc Application of a peptide-mediated magnetic separation-phage assay for detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples
    Antonio Foddai
    Institute of Agri food and Land Use, School of Biological Sciences, Queen s University Belfast, Medical Biology Centre, Belfast, Northern Ireland, UK
    J Clin Microbiol 49:2017-9. 2011
    ..Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay...
  28. pmc Optimization of a phage amplification assay to permit accurate enumeration of viable Mycobacterium avium subsp. paratuberculosis cells
    Antonio Foddai
    Institute of Agri food and Land Use, Queen s University Belfast, Medical Biology Centre, Northern Ireland, United Kingdom
    Appl Environ Microbiol 75:3896-902. 2009
    ..smegmatis mc(2) 155 in 7H9 agar. The optimized phage amplification assay was able to detect 1 to 10 CFU/ml of M. avium subsp. paratuberculosis in spiked milk or broth within 48 h, as demonstrated by the results of several blind trials...
  29. pmc Rapid and sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separation-conventional PCR and real-time PCR
    Sangeeta Khare
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843 4467, USA
    J Clin Microbiol 42:1075-81. 2004
    ....
  30. pmc Surveillance of bulk raw and commercially pasteurized cows' milk from approved Irish liquid-milk pasteurization plants to determine the incidence of Mycobacterium paratuberculosis
    Ciara E O'Reilly
    Food Safety Authority of Ireland, Abbey Court, Lower Abbey St, Dublin 1, Ireland
    Appl Environ Microbiol 70:5138-44. 2004
    ..However, since no viable M. paratuberculosis was isolated from commercially pasteurized cows' milk on retail sale in the Republic of Ireland, current pasteurization procedures are considered to be effective...