Conrad W Mullineaux

Summary

Affiliation: Queen Mary
Country: UK

Publications

  1. ncbi request reprint Probing the dynamics of photosynthetic membranes with fluorescence recovery after photobleaching
    Conrad W Mullineaux
    Dept of Biology, University College London, London, UK
    Trends Plant Sci 7:237-40. 2002
  2. ncbi request reprint Co-existence of photosynthetic and respiratory activities in cyanobacterial thylakoid membranes
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK Electronic address
    Biochim Biophys Acta 1837:503-11. 2014
  3. pmc The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
    S Shawn Morrison
    Department of Basic Medical Sciences, Biochemistry Section, The University of the West Indies, Mona Campus, Kingston 7, Jamaica
    BMC Microbiol 5:47. 2005
  4. doi request reprint Factors controlling the mobility of photosynthetic proteins
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, London, UK
    Photochem Photobiol 84:1310-6. 2008
  5. pmc Mechanism of intercellular molecular exchange in heterocyst-forming cyanobacteria
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, London, UK
    EMBO J 27:1299-308. 2008
  6. ncbi request reprint Phycobilisome-reaction centre interaction in cyanobacteria
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Photosynth Res 95:175-82. 2008
  7. pmc Diffusion of green fluorescent protein in three cell environments in Escherichia coli
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, UK
    J Bacteriol 188:3442-8. 2006
  8. ncbi request reprint Function and evolution of grana
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Trends Plant Sci 10:521-5. 2005
  9. pmc Photoprotective energy dissipation involves the reorganization of photosystem II light-harvesting complexes in the grana membranes of spinach chloroplasts
    Matthew P Johnson
    School of Biological and Chemical Sciences, Queen Mary University of London, London E14NS, United Kingdom
    Plant Cell 23:1468-79. 2011
  10. doi request reprint Clustering and dynamics of cytochrome bd-I complexes in the Escherichia coli plasma membrane in vivo
    Tchern Lenn
    School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Mol Microbiol 70:1397-407. 2008

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Probing the dynamics of photosynthetic membranes with fluorescence recovery after photobleaching
    Conrad W Mullineaux
    Dept of Biology, University College London, London, UK
    Trends Plant Sci 7:237-40. 2002
    ..Recent technological advances are making it increasingly easy to probe the dynamics of photosynthetic membranes using fluorescence recovery after photobleaching. Here we explain the potential and the limitations of the technique...
  2. ncbi request reprint Co-existence of photosynthetic and respiratory activities in cyanobacterial thylakoid membranes
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK Electronic address
    Biochim Biophys Acta 1837:503-11. 2014
    ..This article is part of a Special Issue entitled: Dynamic and ultrastructure of bioenergetic membranes and their components...
  3. pmc The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
    S Shawn Morrison
    Department of Basic Medical Sciences, Biochemistry Section, The University of the West Indies, Mona Campus, Kingston 7, Jamaica
    BMC Microbiol 5:47. 2005
    ..The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria...
  4. doi request reprint Factors controlling the mobility of photosynthetic proteins
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, London, UK
    Photochem Photobiol 84:1310-6. 2008
    ..However, there is sufficient free space to allow the long-range diffusion of some complexes. Finally, I discuss some future directions for research in this area...
  5. pmc Mechanism of intercellular molecular exchange in heterocyst-forming cyanobacteria
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, London, UK
    EMBO J 27:1299-308. 2008
    ..The phenotype of a null mutant identifies FraG (SepJ), a membrane protein localised at the cell-cell interface, as a strong candidate for the channel-forming protein...
  6. ncbi request reprint Phycobilisome-reaction centre interaction in cyanobacteria
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Photosynth Res 95:175-82. 2008
    ....
  7. pmc Diffusion of green fluorescent protein in three cell environments in Escherichia coli
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, UK
    J Bacteriol 188:3442-8. 2006
    ..coli periplasm. However, we measured a GFP diffusion coefficient only slightly smaller in the periplasm than that in the cytoplasm, showing that both cell compartments are relatively fluid environments...
  8. ncbi request reprint Function and evolution of grana
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Trends Plant Sci 10:521-5. 2005
    ..Other organisms solve this problem in different ways. Land plants evolved from macroalgae that were adapted to high light conditions; they evolved grana as a new solution to the problem of efficient photosynthesis in shade...
  9. pmc Photoprotective energy dissipation involves the reorganization of photosystem II light-harvesting complexes in the grana membranes of spinach chloroplasts
    Matthew P Johnson
    School of Biological and Chemical Sciences, Queen Mary University of London, London E14NS, United Kingdom
    Plant Cell 23:1468-79. 2011
    ..It is demonstrated that these changes occur rapidly and reversibly within 5 min of illumination and dark relaxation, are dependent on ΔpH, and are enhanced by the deepoxidation of violaxanthin to zeaxanthin...
  10. doi request reprint Clustering and dynamics of cytochrome bd-I complexes in the Escherichia coli plasma membrane in vivo
    Tchern Lenn
    School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Mol Microbiol 70:1397-407. 2008
    ..coli plasma membrane. We hypothesize that respiration occurs in mobile membrane patches which we call 'respirazones'...
  11. doi request reprint Visualizing the mobility and distribution of chlorophyll proteins in higher plant thylakoid membranes: effects of photoinhibition and protein phosphorylation
    Tomasz K Goral
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK
    Plant J 62:948-59. 2010
    ..We conclude that protein phosphorylation switches the membrane system to a more fluid state, thus facilitating the photosystem-II repair cycle...
  12. pmc Control of electron transport routes through redox-regulated redistribution of respiratory complexes
    Lu Ning Liu
    School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, United Kingdom
    Proc Natl Acad Sci U S A 109:11431-6. 2012
    ....
  13. pmc Loss of the SPHF homologue Slr1768 leads to a catastrophic failure in the maintenance of thylakoid membranes in Synechocystis sp. PCC 6803
    Samantha J Bryan
    School of Biological and Chemical Sciences, Queen Mary University of London, London, United Kingdom
    PLoS ONE 6:e19625. 2011
    ..The mechanism driving the biogenesis of the thylakoid membranes is still an open question, with only two potential biogenesis factors, Vipp1 and Alb3 currently identified...
  14. ncbi request reprint Localization and mobility of bacterial proteins by confocal microscopy and fluorescence recovery after photobleaching
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, London, UK
    Methods Mol Biol 390:3-15. 2007
    ..The techniques are illustrated with reference to measurements on green fluorescent protein (GFP)-tagged proteins in Escherichia coli...
  15. ncbi request reprint Using fluorescence recovery after photobleaching to measure lipid diffusion in membranes
    Conrad W Mullineaux
    School of Biological and Chemical Sciences, Queen Mary, University of London, UK
    Methods Mol Biol 400:267-75. 2007
    ..Procedures for preparing the biological sample, performing the measurement, and quantitative data analysis are explained...
  16. doi request reprint Light-harvesting antenna composition controls the macrostructure and dynamics of thylakoid membranes in Arabidopsis
    Tomasz K Goral
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E14NS, UK
    Plant J 69:289-301. 2012
    ..Thus the presence of PsbS leads to an increase in the fluidity of the membrane, accelerating the re-organization of the photosystem II macrostructure that is necessary for induction of non-photochemical quenching...
  17. pmc Size dependence of protein diffusion in the cytoplasm of Escherichia coli
    Anja Nenninger
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, United Kingdom
    J Bacteriol 192:4535-40. 2010
    ..Two indigenous E. coli proteins were used to show that their specific interactions within the cell are the main controllers of the diffusion rate...
  18. pmc Mobilization of photosystem II induced by intense red light in the Cyanobacterium Synechococcus sp PCC7942
    Mary Sarcina
    School of Biological Sciences, Queen Mary, University of London, London E1 4NS, United Kingdom
    Plant Cell 18:457-64. 2006
    ..The localized concentrations of photosystem II seen after red light treatment may correspond to specific zones where the repair cycle is active...
  19. doi request reprint Branching and intercellular communication in the Section V cyanobacterium Mastigocladus laminosus, a complex multicellular prokaryote
    Dennis J Nürnberg
    School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, E1 4NS, UK
    Mol Microbiol 91:935-49. 2014
    ..Cells in young, narrow trichomes and hormogonia exhibited faster exchange rates than cells in older, wider trichomes. Signal transduction to co-ordinate movement of hormogonia might be accelerated by reducing cell volume. ..
  20. doi request reprint Are Escherichia coli OXPHOS complexes concentrated in specialized zones within the plasma membrane?
    Tchern Lenn
    School of Biological and Chemical Sciences, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Biochem Soc Trans 36:1032-6. 2008
    ..The formulation and physiological significance of this hypothesis are discussed in this paper...
  21. doi request reprint Degradation of cyanobacterial biosignatures by ionizing radiation
    Lewis R Dartnell
    UCL Institute for Origins, University College London, London, UK
    Astrobiology 11:997-1016. 2011
    ..This allows estimation of the degradation rates of cyanobacterial biosignatures on the martian surface and the identification of promising detectable fluorescent break-down products...
  22. doi request reprint 2-epi-5-epi-Valiolone synthase activity is essential for maintaining phycobilisome composition in the cyanobacterium Anabaena variabilis ATCC 29413 when grown in the presence of a carbon source
    Edward Spence
    Institute of Pharmaceutical Science, King s College London, 150 Stamford Street, London, SE1 9NH, UK
    Photosynth Res 116:33-43. 2013
    ..A role of EVS in regulating sedoheptulose 7-phosphate concentrations in the photophysiology of coral symbiosis is yet to be determined. ..
  23. ncbi request reprint Mobility of the IsiA chlorophyll-binding protein in cyanobacterial thylakoid membranes
    Mary Sarcina
    Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 279:36514-8. 2004
    ..We discuss the implications for the structure and function of the cyanobacterial thylakoid membrane...
  24. ncbi request reprint The ycf27 genes from cyanobacteria and eukaryotic algae: distribution and implications for chloroplast evolution
    Mark K Ashby
    Department of Basic Medical Sciences, Biochemistry Section, University of the West Indies, Kingston, Jamaica
    FEMS Microbiol Lett 214:25-30. 2002
    ..The implications for chloroplast evolution are discussed along with the possible identity of the cognate histidine kinase gene in the plastid genomes...
  25. ncbi request reprint Membrane-specific targeting of green fluorescent protein by the Tat pathway in the cyanobacterium Synechocystis PCC6803
    Edward Spence
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AK, UK
    Mol Microbiol 48:1481-9. 2003
    ....
  26. pmc Protein diffusion and macromolecular crowding in thylakoid membranes
    Helmut Kirchhoff
    Institute of Botany, 48149 Munster, Germany
    Plant Physiol 146:1571-8. 2008
    ..Manipulation of the protein-lipid ratio and the ionic strength of the buffer reveals the roles of macromolecular crowding and protein-protein interactions in restricting the mobility of grana proteins...
  27. pmc FtsH is involved in the early stages of repair of photosystem II in Synechocystis sp PCC 6803
    Paulo Silva
    Department of Biological Sciences, Imperial College London, South Kensington Campus SW7 2AZ, United Kingdom
    Plant Cell 15:2152-64. 2003
    ..These results, together with the recent analysis of ftsH mutants of Arabidopsis, highlight the critical role played by FtsH proteases in the removal of damaged D1 from the membrane and the maintenance of PSII activity in vivo...
  28. pmc Phycobilisome diffusion is required for light-state transitions in cyanobacteria
    Sarah Joshua
    Department of Biology, University College London, London WC1E 6BT, United Kingdom
    Plant Physiol 135:2112-9. 2004
    ..This implies that phycobilisome diffusion is required for state transitions. The main physiological role for phycobilisome mobility may be to allow such flexibility in light harvesting...
  29. ncbi request reprint The FtsH protease slr0228 is important for quality control of photosystem II in the thylakoid membrane of Synechocystis sp. PCC 6803
    Josef Komenda
    Institute of Microbiology, Academy of Sciences, Opatovicky mlyn, Trebon, Czech Republic
    J Biol Chem 281:1145-51. 2006
    ..Overall, our results show that FtsH (slr0228) plays an important role in controlling the removal of PSII subunits from the thylakoid membrane and is not restricted to selective D1 turnover...
  30. ncbi request reprint Lipid diffusion in the thylakoid membranes of the cyanobacterium Synechococcus sp.: effect of fatty acid desaturation
    Mary Sarcina
    Department of Biology, University College London, Darwin Building, Gower Street, WC1E 6BT London, UK
    FEBS Lett 553:295-8. 2003
    ..The transformant showed a six-fold increase in the diffusion coefficient for the fluorescent marker at growth temperature. This is the first direct measurement of lipid diffusion in a photosynthetic membrane...
  31. ncbi request reprint FRAP analysis of photosynthetic membranes
    Conrad W Mullineaux
    Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    J Exp Bot 55:1207-11. 2004
    ..This review explains the potential and the problems, and gives a brief guide to performing FRAP measurements and analysing the data...
  32. ncbi request reprint State transitions: an example of acclimation to low-light stress
    Conrad W Mullineaux
    Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    J Exp Bot 56:389-93. 2005
    ..Thus state transitions are a way to maximize the efficiency of light-harvesting at low light intensities...
  33. ncbi request reprint Location and mobility of twin arginine translocase subunits in the Escherichia coli plasma membrane
    Nicola Ray
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Biol Chem 280:17961-8. 2005
    ..The significance of the punctate TatA/B/C-GFP is unclear; fluorescence recovery after photobleaching measurements show that these pools of proteins are immobile, whereas nonaggregated proteins are highly mobile in the plasma membrane...
  34. ncbi request reprint The trimeric organisation of photosystem I is not necessary for the iron-stress induced CP43' protein to functionally associate with this reaction centre
    Caroline L Aspinwall
    Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    FEBS Lett 574:126-30. 2004
    ..We therefore conclude that the trimeric nature of cyanobacterial PSI is not required for the assembly of the CP43' antenna system under iron-deficient conditions...
  35. pmc Involvement of phycobilisome diffusion in energy quenching in cyanobacteria
    Sarah Joshua
    Department of Biology, University College London, London WC1E 6BT United Kingdom
    Plant Physiol 138:1577-85. 2005
    ..We propose as a working hypothesis that a major component of the fluorescence quenching observed in iron-starved cyanobacteria arises from the coupling of free phycobilisomes to IsiA...
  36. ncbi request reprint The rpaC gene product regulates phycobilisome-photosystem II interaction in cyanobacteria
    Sarah Joshua
    Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    Biochim Biophys Acta 1709:58-68. 2005
    ..Based on these results, we propose that the rpaC gene product controls the stability of the phycobilisome-Photosystem II supercomplex, and is probably a structural component of the complex...
  37. ncbi request reprint The PsbU subunit of photosystem II stabilizes energy transfer and primary photochemistry in the phycobilisome-photosystem II assembly of Synechocystis sp. PCC 6803
    John Veerman
    Department of Biological Sciences, Brock University, St Catharines, Ontario L2S 3A1, Canada
    Biochemistry 44:16939-48. 2005
    ..Our results are consistent with the assumption that PsbU stabilizes both energy transfer and electron transport in the PBS/PSII assembly...
  38. ncbi request reprint Photophysical properties of Prochlorococcus marinus SS120 divinyl chlorophylls and phycoerythrin in vitro and in vivo
    Claudia Steglich
    Institut für Biologie Genetik, Humboldt Universitat zu Berlin, Germany
    FEBS Lett 553:79-84. 2003
    ..Thus, the capacity of PE to function primarily as a photosynthetic light-harvesting pigment in P. marinus SS120 is low...
  39. ncbi request reprint A critical role for the Var2 FtsH homologue of Arabidopsis thaliana in the photosystem II repair cycle in vivo
    Shaun Bailey
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, London WC1E 6BT, United Kingdom
    J Biol Chem 277:2006-11. 2002
    ..We conclude, therefore, that the Var2 FtsH homologue is required for the cleavage of the D1 polypeptide in vivo. In addition, we identify a conserved lumenal domain in Var2 that is unique to FtsH homologues from oxygenic phototrophs...