Research Topics
| David A LeeSummaryAffiliation: Queen Mary Country: UK Publications
| Collaborators
|
Detail Information
Publications
Mechanical regulation of nuclear structure and functionRui P Martins
Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, United Kingdom
Annu Rev Biomed Eng 14:431-55. 2012..These phenomena are discussed in relation to the potential influence of nuclear architecture-mediated mechanoregulation of transcription and cell fate...- Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructsT T Chowdhury
School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
Arthritis Res Ther 10:R35. 2008.... 
Stem cell mechanobiologyDavid A Lee
University of London, London E1 4NS, UK
J Cell Biochem 112:1-9. 2011....
Cellular utilization determines viability and matrix distribution profiles in chondrocyte-seeded alginate constructsHannah K Heywood
Medical Engineering Division and Interdisciplinary Research Centre in Biomedical Materials, Department of Engineering, Queen Mary, University of London, London, UK
Tissue Eng 10:1467-79. 2004..This work indicates that cellular utilization determines the nonhomogeneous nature of cartilage formation in three-dimensional constructs and presents a guide to nonlimiting medium volumes for static culture conditions...- Cyclic tensile strain upregulates collagen synthesis in isolated tendon fasciclesHazel R C Screen
Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
Biochem Biophys Res Commun 336:424-9. 2005..These data provide evidence of altered anabolic activity within tendon in response to mechanical stimuli, and suggest the importance of cyclic tensile loading for the maintenance of the collagen hierarchy within tendon... - Differential regulation of gene expression in isolated tendon fascicles exposed to cyclic tensile strain in vitroEijiro Maeda
School of Engineering and Materials Science, Queen Mary, Uniersity of London, London, E1 4NS, UK
J Appl Physiol 106:506-12. 2009..These findings demonstrate distinctive patterns of mechanoregulation for anabolic and catabolic genes and help our understanding of tenocyte response to mechanical stimulation... - Dynamic compressive strain influences chondrogenic gene expression in human mesenchymal stem cellsJonathan J Campbell
Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary University of London, London, UK
Biorheology 43:455-70. 2006..However, the combined effects of strain and TGFbeta on these markers were more complex than purely additive... - The development of a bioreactor to perfuse radially-confined hydrogel constructs: design and characterization of mass transport propertiesJoshua O Eniwumide
Medical Engineering Division and IRC in Biomedical Materials, School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, UK
Biorheology 46:417-37. 2009..More significantly was the 106% and 1603% increase of DNA and GAG, respectively, achieved at the central core of the 3D constructs... - Glucose concentration and medium volume influence cell viability and glycosaminoglycan synthesis in chondrocyte-seeded alginate constructsHannah K Heywood
Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary University of London, London, UK
Tissue Eng 12:3487-96. 2006..8 to 3.2 mL/10(-6) cells, when maximal values of construct GAG content and mass were obtained. Therefore, medium volume remains an important consideration for the optimal culture of tissue-engineered cartilage... - The metabolism of human mesenchymal stem cells during proliferation and differentiationGirish Pattappa
School of Engineering and Materials Science, Queen Mary University of London, London, UK
J Cell Physiol 226:2562-70. 2011..Thus, the differences between the energy metabolism of chondrogenic and osteogenic cultures may be associated with the culture conditions and not necessarily their respective differentiation... - Cell-generated forces influence the viability, metabolism and mechanical properties of fibroblast-seeded collagen gel constructsCatherine C Berry
IRC in Biomedical Materials, School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
J Tissue Eng Regen Med 3:43-53. 2009..The 10 mm model system induced a high level of cell and matrix orientation and may, therefore, represent the best option for tissue-engineered ligament repair involving an orientated fibroblast-seeded collagen gel... - Time dependence of cyclic tensile strain on collagen production in tendon fasciclesEijiro Maeda
Medical Engineering Division and IRC in Biomedical Materials, School of Engineering and Material Science, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
Biochem Biophys Res Commun 362:399-404. 2007..These results indicate that collagen synthesis by tenocytes is controlled by a complex mechanosensitive process with a temporal component... - Functional analysis of tenocytes gene expression in tendon fascicles subjected to cyclic tensile strainEijiro Maeda
Medical Engineering Division and IRC in Biomedical Materials, School of Engineering and Materials Science, Queen Mary, University of London, London, UK
Connect Tissue Res 51:434-44. 2010..The present results suggest that the metabolic balance was shifted in favor of catabolism by the application of a small number of tensile strain cycles, whereas an extended number stimulates strong anti-catabolic effects... - Gap junction permeability between tenocytes within tendon fascicles is suppressed by tensile loadingEijiro Maeda
School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
Biomech Model Mechanobiol 11:439-47. 2012..This is therefore the first study to show that tenocyte gap junctions are not only important in transmitting mechanically activated signals but that mechanical loading directly regulates gap junction permeability... - Dynamic compression counteracts IL-1beta induced iNOS and COX-2 activity by human chondrocytes cultured in agarose constructsTina T Chowdhury
Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
Biorheology 43:413-29. 2006..These data indicate interactions exist between the NOS and COX pathways, a finding which will provide new insights in the development of pharmacological or biophysical treatments for cartilage disorders such as OA... - The influence of swelling and matrix degradation on the microstructural integrity of tendonHazel R C Screen
Department of Engineering, Medical Engineering Division and IRC in Biomedical Materials, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
Acta Biomater 2:505-13. 2006..It also highlights the necessity to maintain samples within a suitable and controlled environment prior to testing... - Single photon counting fluorescence lifetime detection of pericellular oxygen concentrationsNeveen A Hosny
University of London, School of Engineering and Materials Science, Queen Mary, London, United Kingdom, E1 4NS
J Biomed Opt 17:016007. 2012..This technique provides a powerful tool for quantifying spatial oxygen gradients within three-dimensional cellular models... - Both superficial and deep zone articular chondrocyte subpopulations exhibit the Crabtree effect but have different basal oxygen consumption ratesHannah K Heywood
School of Engineering and Materials Science, Queen Mary University of London, London, UK
J Cell Physiol 223:630-9. 2010..The work has further application to the optimisation of bioreactor design and engineered tissues... - Low oxygen reduces the modulation to an oxidative phenotype in monolayer-expanded chondrocytesHannah K Heywood
School of Engineering and Materials Science, Queen Mary University of London, London, UK
J Cell Physiol 222:248-53. 2010..Although the shift towards an oxidative energy metabolism is often accompanied by morphological changes, this study does not support the hypothesis that it is driven by them... - Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strainDavid A Lee
IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary College, University of London, London, UK
Methods Mol Med 100:307-24. 2004..The chapter thus provides an introduction to the experimental techniques used to examine chondrocyte mechanotransduction and downstream cell function... - Influence of external uniaxial cyclic strain on oriented fibroblast-seeded collagen gelsCatherine C Berry
IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, London E1 4NS, UK
Tissue Eng 9:613-24. 2003..By contrast, the initial failure loads for cyclically strained constructs preloaded at 10 mN were reduced, potentially because of enhanced catabolic activity... - Quantification of mRNA using real-time PCR and Western blot analysis of MAPK events in chondrocyte/agarose constructsDavid A Lee
School of Engineering and Materials Science, Queen Mary University of London, London, UK
Methods Mol Biol 695:77-97. 2011..Ultimately, development of methods used to analyse mechano-sensitive pathways will provide important information for the identification of appropriate pharmacological and physiotherapeutic agents for the treatment of osteoarthritis... - Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compressionTina T Chowdhury
Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
Arch Biochem Biophys 417:105-11. 2003..The uncoupled nature of the metabolic response determined in this study suggests that mechanical conditioning regimes may be fine tuned to selectively stimulate key metabolic parameters of relevance to cartilage tissue engineering... - Live cell imaging using confocal microscopy induces intracellular calcium transients and cell deathMartin M Knight
Interdisciplinary Research Centre, Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, United Kingdom
Am J Physiol Cell Physiol 284:C1083-9. 2003..Light-activated Ca2+ signaling may trigger alterations in numerous cell processes and thereby represent an important and hitherto overlooked artifact in fluorescent microscopy of viable cells... - The influence of noncollagenous matrix components on the micromechanical environment of tendon fasciclesHazel R C Screen
Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, London, E1 4NS, United Kingdom
Ann Biomed Eng 33:1090-9. 2005..This highlights the importance of maintaining fascicles within their natural environment to prevent structural or mechanical changes prior to subsequent analysis... - Expansion of chondrocytes for tissue engineering in alginate beads enhances chondrocytic phenotype compared to conventional monolayer techniquesDavid A Lee
IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
Acta Orthop Scand 74:6-15. 2003..In conclusion, expansion of cells for autologous cartilage repair systems, using subculture within alginate beads, provides a potentially attractive alternative to monolayer expansion... - Mechanical conditioning influences the metabolic response of cell-seeded constructsJulia C Shelton
Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, London, UK
Cells Tissues Organs 175:140-50. 2003..The other critical factors in determining response to both types of conditioning are associated with the age of the cell and its original location within the tissue...