David A Lee

Summary

Affiliation: Queen Mary
Country: UK

Publications

  1. pmc Mechanical regulation of nuclear structure and function
    Rui P Martins
    Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, United Kingdom
    Annu Rev Biomed Eng 14:431-55. 2012
  2. pmc Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs
    T T Chowdhury
    School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Arthritis Res Ther 10:R35. 2008
  3. doi request reprint Stem cell mechanobiology
    David A Lee
    University of London, London E1 4NS, UK
    J Cell Biochem 112:1-9. 2011
  4. ncbi request reprint Cellular utilization determines viability and matrix distribution profiles in chondrocyte-seeded alginate constructs
    Hannah K Heywood
    Medical Engineering Division and Interdisciplinary Research Centre in Biomedical Materials, Department of Engineering, Queen Mary, University of London, London, UK
    Tissue Eng 10:1467-79. 2004
  5. ncbi request reprint Cyclic tensile strain upregulates collagen synthesis in isolated tendon fascicles
    Hazel R C Screen
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Biochem Biophys Res Commun 336:424-9. 2005
  6. doi request reprint Differential regulation of gene expression in isolated tendon fascicles exposed to cyclic tensile strain in vitro
    Eijiro Maeda
    School of Engineering and Materials Science, Queen Mary, Uniersity of London, London, E1 4NS, UK
    J Appl Physiol 106:506-12. 2009
  7. ncbi request reprint Dynamic compressive strain influences chondrogenic gene expression in human mesenchymal stem cells
    Jonathan J Campbell
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary University of London, London, UK
    Biorheology 43:455-70. 2006
  8. doi request reprint The development of a bioreactor to perfuse radially-confined hydrogel constructs: design and characterization of mass transport properties
    Joshua O Eniwumide
    Medical Engineering Division and IRC in Biomedical Materials, School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, UK
    Biorheology 46:417-37. 2009
  9. ncbi request reprint Glucose concentration and medium volume influence cell viability and glycosaminoglycan synthesis in chondrocyte-seeded alginate constructs
    Hannah K Heywood
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary University of London, London, UK
    Tissue Eng 12:3487-96. 2006
  10. doi request reprint The metabolism of human mesenchymal stem cells during proliferation and differentiation
    Girish Pattappa
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    J Cell Physiol 226:2562-70. 2011

Collaborators

Detail Information

Publications27

  1. pmc Mechanical regulation of nuclear structure and function
    Rui P Martins
    Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, United Kingdom
    Annu Rev Biomed Eng 14:431-55. 2012
    ..These phenomena are discussed in relation to the potential influence of nuclear architecture-mediated mechanoregulation of transcription and cell fate...
  2. pmc Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs
    T T Chowdhury
    School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Arthritis Res Ther 10:R35. 2008
    ....
  3. doi request reprint Stem cell mechanobiology
    David A Lee
    University of London, London E1 4NS, UK
    J Cell Biochem 112:1-9. 2011
    ....
  4. ncbi request reprint Cellular utilization determines viability and matrix distribution profiles in chondrocyte-seeded alginate constructs
    Hannah K Heywood
    Medical Engineering Division and Interdisciplinary Research Centre in Biomedical Materials, Department of Engineering, Queen Mary, University of London, London, UK
    Tissue Eng 10:1467-79. 2004
    ..This work indicates that cellular utilization determines the nonhomogeneous nature of cartilage formation in three-dimensional constructs and presents a guide to nonlimiting medium volumes for static culture conditions...
  5. ncbi request reprint Cyclic tensile strain upregulates collagen synthesis in isolated tendon fascicles
    Hazel R C Screen
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Biochem Biophys Res Commun 336:424-9. 2005
    ..These data provide evidence of altered anabolic activity within tendon in response to mechanical stimuli, and suggest the importance of cyclic tensile loading for the maintenance of the collagen hierarchy within tendon...
  6. doi request reprint Differential regulation of gene expression in isolated tendon fascicles exposed to cyclic tensile strain in vitro
    Eijiro Maeda
    School of Engineering and Materials Science, Queen Mary, Uniersity of London, London, E1 4NS, UK
    J Appl Physiol 106:506-12. 2009
    ..These findings demonstrate distinctive patterns of mechanoregulation for anabolic and catabolic genes and help our understanding of tenocyte response to mechanical stimulation...
  7. ncbi request reprint Dynamic compressive strain influences chondrogenic gene expression in human mesenchymal stem cells
    Jonathan J Campbell
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary University of London, London, UK
    Biorheology 43:455-70. 2006
    ..However, the combined effects of strain and TGFbeta on these markers were more complex than purely additive...
  8. doi request reprint The development of a bioreactor to perfuse radially-confined hydrogel constructs: design and characterization of mass transport properties
    Joshua O Eniwumide
    Medical Engineering Division and IRC in Biomedical Materials, School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, UK
    Biorheology 46:417-37. 2009
    ..More significantly was the 106% and 1603% increase of DNA and GAG, respectively, achieved at the central core of the 3D constructs...
  9. ncbi request reprint Glucose concentration and medium volume influence cell viability and glycosaminoglycan synthesis in chondrocyte-seeded alginate constructs
    Hannah K Heywood
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary University of London, London, UK
    Tissue Eng 12:3487-96. 2006
    ..8 to 3.2 mL/10(-6) cells, when maximal values of construct GAG content and mass were obtained. Therefore, medium volume remains an important consideration for the optimal culture of tissue-engineered cartilage...
  10. doi request reprint The metabolism of human mesenchymal stem cells during proliferation and differentiation
    Girish Pattappa
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    J Cell Physiol 226:2562-70. 2011
    ..Thus, the differences between the energy metabolism of chondrogenic and osteogenic cultures may be associated with the culture conditions and not necessarily their respective differentiation...
  11. doi request reprint Cell-generated forces influence the viability, metabolism and mechanical properties of fibroblast-seeded collagen gel constructs
    Catherine C Berry
    IRC in Biomedical Materials, School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    J Tissue Eng Regen Med 3:43-53. 2009
    ..The 10 mm model system induced a high level of cell and matrix orientation and may, therefore, represent the best option for tissue-engineered ligament repair involving an orientated fibroblast-seeded collagen gel...
  12. ncbi request reprint Time dependence of cyclic tensile strain on collagen production in tendon fascicles
    Eijiro Maeda
    Medical Engineering Division and IRC in Biomedical Materials, School of Engineering and Material Science, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Biochem Biophys Res Commun 362:399-404. 2007
    ..These results indicate that collagen synthesis by tenocytes is controlled by a complex mechanosensitive process with a temporal component...
  13. doi request reprint Functional analysis of tenocytes gene expression in tendon fascicles subjected to cyclic tensile strain
    Eijiro Maeda
    Medical Engineering Division and IRC in Biomedical Materials, School of Engineering and Materials Science, Queen Mary, University of London, London, UK
    Connect Tissue Res 51:434-44. 2010
    ..The present results suggest that the metabolic balance was shifted in favor of catabolism by the application of a small number of tensile strain cycles, whereas an extended number stimulates strong anti-catabolic effects...
  14. doi request reprint Gap junction permeability between tenocytes within tendon fascicles is suppressed by tensile loading
    Eijiro Maeda
    School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Biomech Model Mechanobiol 11:439-47. 2012
    ..This is therefore the first study to show that tenocyte gap junctions are not only important in transmitting mechanically activated signals but that mechanical loading directly regulates gap junction permeability...
  15. ncbi request reprint Dynamic compression counteracts IL-1beta induced iNOS and COX-2 activity by human chondrocytes cultured in agarose constructs
    Tina T Chowdhury
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Biorheology 43:413-29. 2006
    ..These data indicate interactions exist between the NOS and COX pathways, a finding which will provide new insights in the development of pharmacological or biophysical treatments for cartilage disorders such as OA...
  16. ncbi request reprint The influence of swelling and matrix degradation on the microstructural integrity of tendon
    Hazel R C Screen
    Department of Engineering, Medical Engineering Division and IRC in Biomedical Materials, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Acta Biomater 2:505-13. 2006
    ..It also highlights the necessity to maintain samples within a suitable and controlled environment prior to testing...
  17. doi request reprint Single photon counting fluorescence lifetime detection of pericellular oxygen concentrations
    Neveen A Hosny
    University of London, School of Engineering and Materials Science, Queen Mary, London, United Kingdom, E1 4NS
    J Biomed Opt 17:016007. 2012
    ..This technique provides a powerful tool for quantifying spatial oxygen gradients within three-dimensional cellular models...
  18. doi request reprint Both superficial and deep zone articular chondrocyte subpopulations exhibit the Crabtree effect but have different basal oxygen consumption rates
    Hannah K Heywood
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    J Cell Physiol 223:630-9. 2010
    ..The work has further application to the optimisation of bioreactor design and engineered tissues...
  19. doi request reprint Low oxygen reduces the modulation to an oxidative phenotype in monolayer-expanded chondrocytes
    Hannah K Heywood
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    J Cell Physiol 222:248-53. 2010
    ..Although the shift towards an oxidative energy metabolism is often accompanied by morphological changes, this study does not support the hypothesis that it is driven by them...
  20. ncbi request reprint Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strain
    David A Lee
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary College, University of London, London, UK
    Methods Mol Med 100:307-24. 2004
    ..The chapter thus provides an introduction to the experimental techniques used to examine chondrocyte mechanotransduction and downstream cell function...
  21. ncbi request reprint Influence of external uniaxial cyclic strain on oriented fibroblast-seeded collagen gels
    Catherine C Berry
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, London E1 4NS, UK
    Tissue Eng 9:613-24. 2003
    ..By contrast, the initial failure loads for cyclically strained constructs preloaded at 10 mN were reduced, potentially because of enhanced catabolic activity...
  22. doi request reprint Quantification of mRNA using real-time PCR and Western blot analysis of MAPK events in chondrocyte/agarose constructs
    David A Lee
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    Methods Mol Biol 695:77-97. 2011
    ..Ultimately, development of methods used to analyse mechano-sensitive pathways will provide important information for the identification of appropriate pharmacological and physiotherapeutic agents for the treatment of osteoarthritis...
  23. ncbi request reprint Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression
    Tina T Chowdhury
    Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London E1 4NS, UK
    Arch Biochem Biophys 417:105-11. 2003
    ..The uncoupled nature of the metabolic response determined in this study suggests that mechanical conditioning regimes may be fine tuned to selectively stimulate key metabolic parameters of relevance to cartilage tissue engineering...
  24. ncbi request reprint Live cell imaging using confocal microscopy induces intracellular calcium transients and cell death
    Martin M Knight
    Interdisciplinary Research Centre, Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, United Kingdom
    Am J Physiol Cell Physiol 284:C1083-9. 2003
    ..Light-activated Ca2+ signaling may trigger alterations in numerous cell processes and thereby represent an important and hitherto overlooked artifact in fluorescent microscopy of viable cells...
  25. ncbi request reprint The influence of noncollagenous matrix components on the micromechanical environment of tendon fascicles
    Hazel R C Screen
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, London, E1 4NS, United Kingdom
    Ann Biomed Eng 33:1090-9. 2005
    ..This highlights the importance of maintaining fascicles within their natural environment to prevent structural or mechanical changes prior to subsequent analysis...
  26. ncbi request reprint Expansion of chondrocytes for tissue engineering in alginate beads enhances chondrocytic phenotype compared to conventional monolayer techniques
    David A Lee
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Acta Orthop Scand 74:6-15. 2003
    ..In conclusion, expansion of cells for autologous cartilage repair systems, using subculture within alginate beads, provides a potentially attractive alternative to monolayer expansion...
  27. ncbi request reprint Mechanical conditioning influences the metabolic response of cell-seeded constructs
    Julia C Shelton
    Medical Engineering Division and IRC in Biomedical Materials, Department of Engineering, Queen Mary, University of London, London, UK
    Cells Tissues Organs 175:140-50. 2003
    ..The other critical factors in determining response to both types of conditioning are associated with the age of the cell and its original location within the tissue...