Martin M Knight

Summary

Affiliation: Queen Mary
Country: UK

Publications

  1. pmc Depletion of chondrocyte primary cilia reduces the compressive modulus of articular cartilage
    Jerome Irianto
    Institute of Bioengineering and School of Engineering and Materials Science, Queen Mary University of London, Mile End Rd, London E1 4NS, United Kingdom
    J Biomech 47:579-82. 2014
  2. pmc Viscoelastic cell mechanics and actin remodelling are dependent on the rate of applied pressure
    Priyanka Pravincumar
    Institute of Bioengineering, School of Engineering and Material Science, Queen Mary University of London, London, United Kingdom
    PLoS ONE 7:e43938. 2012
  3. pmc The potential of pulsed low intensity ultrasound to stimulate chondrocytes matrix synthesis in agarose and monolayer cultures
    Natalie M Vaughan
    School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK
    Med Biol Eng Comput 48:1215-22. 2010
  4. pmc Articular chondrocytes express connexin 43 hemichannels and P2 receptors - a putative mechanoreceptor complex involving the primary cilium?
    M M Knight
    Queen Mary University of London, UK
    J Anat 214:275-83. 2009
  5. ncbi request reprint Chondrocyte deformation induces mitochondrial distortion and heterogeneous intracellular strain fields
    M M Knight
    Medical Engineering Division, Dept of Engineering and IRC in Biomedical Materials, Queen Mary University of London, London, UK
    Biomech Model Mechanobiol 5:180-91. 2006
  6. ncbi request reprint Mechanical compression and hydrostatic pressure induce reversible changes in actin cytoskeletal organisation in chondrocytes in agarose
    M M Knight
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, London, UK
    J Biomech 39:1547-51. 2006
  7. ncbi request reprint Cell and nucleus deformation in compressed chondrocyte-alginate constructs: temporal changes and calculation of cell modulus
    M M Knight
    Interdisciplinary Research Centre in Biomedical Materials and Department of Engineering, Queen Mary University of London, Mile End Rd, E1 4NS, London, UK
    Biochim Biophys Acta 1570:1-8. 2002
  8. ncbi request reprint Live cell imaging using confocal microscopy induces intracellular calcium transients and cell death
    Martin M Knight
    Interdisciplinary Research Centre, Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, United Kingdom
    Am J Physiol Cell Physiol 284:C1083-9. 2003
  9. ncbi request reprint Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strain
    David A Lee
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary College, University of London, London, UK
    Methods Mol Med 100:307-24. 2004
  10. ncbi request reprint Intracellular mechanics and mechanotransduction associated with chondrocyte deformation during pipette aspiration
    T Ohashi
    Medical Engineering Division, Department of Engineering, Queen Mary University of London, London, UK
    Biorheology 43:201-14. 2006

Collaborators

Detail Information

Publications35

  1. pmc Depletion of chondrocyte primary cilia reduces the compressive modulus of articular cartilage
    Jerome Irianto
    Institute of Bioengineering and School of Engineering and Materials Science, Queen Mary University of London, Mile End Rd, London E1 4NS, United Kingdom
    J Biomech 47:579-82. 2014
    ..This is consistent with histological analysis and confirms the importance of primary cilia in the development of a mechanically functional articular cartilage. ..
  2. pmc Viscoelastic cell mechanics and actin remodelling are dependent on the rate of applied pressure
    Priyanka Pravincumar
    Institute of Bioengineering, School of Engineering and Material Science, Queen Mary University of London, London, United Kingdom
    PLoS ONE 7:e43938. 2012
    ..This study tested the hypothesis that the rate at which pressure (or stress) is applied influence the viscoelastic properties of the cell associated with differences in the dynamics of the actin cytoskeleton...
  3. pmc The potential of pulsed low intensity ultrasound to stimulate chondrocytes matrix synthesis in agarose and monolayer cultures
    Natalie M Vaughan
    School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK
    Med Biol Eng Comput 48:1215-22. 2010
    ..These results suggest that PLIUS has a limited potential to provide an effective method of stimulating matrix production as part of a tissue engineering strategy for cartilage repair...
  4. pmc Articular chondrocytes express connexin 43 hemichannels and P2 receptors - a putative mechanoreceptor complex involving the primary cilium?
    M M Knight
    Queen Mary University of London, UK
    J Anat 214:275-83. 2009
    ..Furthermore, the expression of connexin 43 on the chondrocyte primary cilium suggests the possible involvement of the cilium in this pathway...
  5. ncbi request reprint Chondrocyte deformation induces mitochondrial distortion and heterogeneous intracellular strain fields
    M M Knight
    Medical Engineering Division, Dept of Engineering and IRC in Biomedical Materials, Queen Mary University of London, London, UK
    Biomech Model Mechanobiol 5:180-91. 2006
    ....
  6. ncbi request reprint Mechanical compression and hydrostatic pressure induce reversible changes in actin cytoskeletal organisation in chondrocytes in agarose
    M M Knight
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, London, UK
    J Biomech 39:1547-51. 2006
    ..This reversible remodelling of actin cytoskeleton may therefore represent a mechanism through which the chondrocyte alters its mechanical properties and mechanosensitivity in response to physiological mechanical loading...
  7. ncbi request reprint Cell and nucleus deformation in compressed chondrocyte-alginate constructs: temporal changes and calculation of cell modulus
    M M Knight
    Interdisciplinary Research Centre in Biomedical Materials and Department of Engineering, Queen Mary University of London, Mile End Rd, E1 4NS, London, UK
    Biochim Biophys Acta 1570:1-8. 2002
    ..This phenomenon enabled the calculation of a stress-strain relationship for the cell with an estimated Young's modulus value of approx. 3 kPa...
  8. ncbi request reprint Live cell imaging using confocal microscopy induces intracellular calcium transients and cell death
    Martin M Knight
    Interdisciplinary Research Centre, Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, United Kingdom
    Am J Physiol Cell Physiol 284:C1083-9. 2003
    ..Light-activated Ca2+ signaling may trigger alterations in numerous cell processes and thereby represent an important and hitherto overlooked artifact in fluorescent microscopy of viable cells...
  9. ncbi request reprint Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strain
    David A Lee
    IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary College, University of London, London, UK
    Methods Mol Med 100:307-24. 2004
    ..The chapter thus provides an introduction to the experimental techniques used to examine chondrocyte mechanotransduction and downstream cell function...
  10. ncbi request reprint Intracellular mechanics and mechanotransduction associated with chondrocyte deformation during pipette aspiration
    T Ohashi
    Medical Engineering Division, Department of Engineering, Queen Mary University of London, London, UK
    Biorheology 43:201-14. 2006
    ..These findings have important implications for our understanding of cell mechanics and mechanotransduction as well as interpretation and modelling of pipette aspiration data...
  11. pmc Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytes
    Angus K T Wann
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    FASEB J 26:1663-71. 2012
    ....
  12. ncbi request reprint Confocal analysis of local and cellular strains in chondrocyte-agarose constructs subjected to mechanical shear
    Yoshinori Sawae
    Cell and Tissue Engineering Laboratories, Queen Mary University of London, London, United Kingdom
    Ann Biomed Eng 32:860-70. 2004
    ..This validated experimental system will enable future studies to examine the influence of shear on chondrocyte function and the associated mechanotransduction signalling pathways...
  13. doi request reprint Cyclic loading opens hemichannels to release ATP as part of a chondrocyte mechanotransduction pathway
    Mariana Garcia
    Queen Mary University of London, School of Engineering and Materials Science, Mile End Road, London E1 4NS, United Kingdom
    J Orthop Res 28:510-5. 2010
    ..In conclusion, mechanical loading triggers release of ATP via hemichannels. Hence, this study provides the first evidence of hemichannel involvement in chondrocyte mechanobiology...
  14. doi request reprint Gap junction permeability between tenocytes within tendon fascicles is suppressed by tensile loading
    Eijiro Maeda
    School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
    Biomech Model Mechanobiol 11:439-47. 2012
    ..This is therefore the first study to show that tenocyte gap junctions are not only important in transmitting mechanically activated signals but that mechanical loading directly regulates gap junction permeability...
  15. doi request reprint Single photon counting fluorescence lifetime detection of pericellular oxygen concentrations
    Neveen A Hosny
    University of London, School of Engineering and Materials Science, Queen Mary, London, United Kingdom, E1 4NS
    J Biomed Opt 17:016007. 2012
    ..This technique provides a powerful tool for quantifying spatial oxygen gradients within three-dimensional cellular models...
  16. ncbi request reprint Deformation properties of articular chondrocytes: a critique of three separate techniques
    D L Bader
    IRC in Biomedical Materials and Medical Engineering Division, Queen Mary, University of London, UK
    Biorheology 39:69-78. 2002
    ..7 kPa. The study highlights the difficulties in establishing unique mechanical parameters, which reflect the deformation behaviour of articular chondrocytes...
  17. doi request reprint Biomechanical analysis of structural deformation in living cells
    D L Bader
    School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, UK
    Med Biol Eng Comput 46:951-63. 2008
    ..The review focuses on deformation of chondrocytes with additional information on a variety of other cell types including neurons, red blood cells, epithelial cells and skin and muscle cells...
  18. ncbi request reprint Activation of chondrocytes calcium signalling by dynamic compression is independent of number of cycles
    B Pingguan-Murphy
    Medical Engineering Division, Department of Engineering and IRC in Biomedical Materials, Queen Mary University of London, London, UK
    Arch Biochem Biophys 444:45-51. 2005
    ..Therefore, Ca(2+) signalling may be part of a mechanotransduction pathway through which chondrocyte populations can modulate their metabolic activity in response to changing mechanical stimuli...
  19. ncbi request reprint Loading alters actin dynamics and up-regulates cofilin gene expression in chondrocytes
    J J Campbell
    Medical Engineering Division, School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK
    Biochem Biophys Res Commun 361:329-34. 2007
    ..Thus mechanical loading alters cortical actin dynamics, providing a potential mechanism through which chondrocytes can adapt their mechanical properties and mechanosensitivity to the local mechanical environment...
  20. pmc Osmotic challenge drives rapid and reversible chromatin condensation in chondrocytes
    Jerome Irianto
    Institute of Bioengineering, School of Engineering and Material Science, Queen Mary, University of London, London, United Kingdom
    Biophys J 104:759-69. 2013
    ..Alterations in chromatin structure are expected to influence gene expression and thereby regulate chondrocyte activity in response to osmotic changes...
  21. doi request reprint Both superficial and deep zone articular chondrocyte subpopulations exhibit the Crabtree effect but have different basal oxygen consumption rates
    Hannah K Heywood
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    J Cell Physiol 223:630-9. 2010
    ..The work has further application to the optimisation of bioreactor design and engineered tissues...
  22. ncbi request reprint Chondrocyte deformation within mechanically and enzymatically extracted chondrons compressed in agarose
    M M Knight
    Interdisciplinary Research Centre in Biomedical Materials, Queen Mary University of London, Mile End Rd, London E1 4NS, UK
    Biochim Biophys Acta 1526:141-6. 2001
    ..Although these differences may be reduced with time in culture, characterisation of mechanically isolated chondrons suggests that the stiffness of the chondrons in situ may be greater than previous estimates...
  23. doi request reprint The effect of mechanical strain or hypoxia on cell death in subpopulations of rat dorsal root ganglion neurons in vitro
    S J Gladman
    Centre for Neuroscience and Trauma, Blizard Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, E1 2AT, UK
    Neuroscience 171:577-87. 2010
    ..These results provide detailed information on the response of adult DRG subpopulations to hypoxia and mechanical strain, and describe in vitro models that could be useful for screening potential neuroprotective agents...
  24. ncbi request reprint Purinergic pathway suppresses the release of .NO and stimulates proteoglycan synthesis in chondrocyte/agarose constructs subjected to dynamic compression
    T T Chowdhury
    Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London, UK
    J Cell Physiol 209:845-53. 2006
    ..The current study demonstrates for the first time the importance of a purinergic pathway in mediating the metabolic response to dynamic compression and suppressing an inflammatory effect...
  25. ncbi request reprint Measurement of the deformation of isolated chondrocytes in agarose subjected to cyclic compression
    M M Knight
    IRC in Biomedical Materials, Queen Mary and Westfield College, London, UK
    Med Eng Phys 20:684-8. 1998
    ..72), although during static compression the cell deformation remained constant. This system may be used to examine cellular events under a range of dynamic mechanical stimuli...
  26. doi request reprint Stem cell mechanobiology
    David A Lee
    University of London, London E1 4NS, UK
    J Cell Biochem 112:1-9. 2011
    ....
  27. pmc Eicosapentaenoic acid and docosahexaenoic acid reduce interleukin-1β-mediated cartilage degradation
    Angus K T Wann
    School of Engineering and Materials Science, Queen Mary University of London, Mile End, London, E1 4NS, UK
    Arthritis Res Ther 12:R207. 2010
    ..The aim of this study was to examine the therapeutic potential of n-3 polyunsaturated fatty acids (PUFAs) in an in vitro model of cartilage inflammation...
  28. pmc Super-resolution imaging strategies for cell biologists using a spinning disk microscope
    Neveen A Hosny
    Blizard Institute, Barts and the Royal London School of Medicine and Dentistry, Queen Mary University London, London, United Kingdom
    PLoS ONE 8:e74604. 2013
    ..We have shown that it was possible to obtain two coloured images, which highlights the potential this technique has for high-content screening, imaging of multiple epitopes and live cell imaging. ..
  29. doi request reprint Quantification of chromatin condensation level by image processing
    Jerome Irianto
    Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary, University of London, London, United Kingdom Electronic address
    Med Eng Phys 36:412-7. 2014
    ..This image processing technique thereby provides a validated unbiased parameter for rapid and highly reproducible quantification of the level of chromatin condensation. ..
  30. pmc Interleukin-1β sequesters hypoxia inducible factor 2α to the primary cilium
    Angus Kt Wann
    Institute of Bioengineering and School of Engineering and Materials Science, Queen Mary University of London, Mile End, London, E1 4NS, UK
    Cilia 2:17. 2013
    ..The present study tests the hypothesis that an association exists between the primary cilium and HIFs in inflammatory signalling...
  31. pmc Heat shock induces rapid resorption of primary cilia
    Natalia V Prodromou
    Centre for Endocrinology, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London EC1M 6BQ, UK
    J Cell Sci 125:4297-305. 2012
    ..We hypothesise that ciliary resorption serves to attenuate cilia-mediated signalling pathways in response to extracellular stress, and that this mechanism is regulated in part by HDAC6 and Hsp90...
  32. doi request reprint Improved outcome after peripheral nerve injury in mice with increased levels of endogenous ω-3 polyunsaturated fatty acids
    Stacy J Gladman
    Centre for Neuroscience and Trauma, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, E1 2AT, UK
    J Neurosci 32:563-71. 2012
    ..Fat-1 animals also had some protection against muscle atrophy after injury. In conclusion, both in vitro and in vivo experiments support the idea that a higher endogenous ω-3 PUFA could lead to beneficial effects after a PNI...
  33. ncbi request reprint An improved confocal FRAP technique for the measurement of long-term actin dynamics in individual stress fibers
    J J Campbell
    Department of Engineering, Medical Engineering Division, Queen Mary University of London, London E1 4NS, United Kingdom
    Microsc Res Tech 70:1034-40. 2007
    ..In conclusion, the present FRAP methodology overcomes many of the limitations of previous studies in order to provide new data describing long-term actin dynamics within individual stress fibers...
  34. ncbi request reprint Mitochondrial dynamics in chondrocytes and their connection to the mechanical properties of the cytoplasm
    Ze ev Bomzon
    Faculty of Civil and Environmental Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
    J Biomech Eng 128:674-9. 2006
    ..This paper presents a study that characterizes the diffusive motion of mitochondria in chondrocytes seeded in agarose constructs and what this implies about the mechanical properties of the cytoplasm...
  35. ncbi request reprint Ultrastructure of insect and spider cocoon silks
    Osnat Hakimi
    Interdisciplinary Research Center in Biomedical Materials, Materials Department, and Center for Academic Surgery, Queen Mary University of London, London E1 4NS o hakimi qmul ac uk
    Biomacromolecules 7:2901-8. 2006
    ..Observed separation of the microfibrils in fractured brins suggests that the multifibrillar structure of the silk fiber contributes to toughness by allowing dissipation of energy in the controlled propagation of cracks...