Martin M Knight
Affiliation: Queen Mary
- Chondrocyte deformation induces mitochondrial distortion and heterogeneous intracellular strain fieldsM M Knight
Medical Engineering Division, Dept of Engineering and IRC in Biomedical Materials, Queen Mary University of London, London, UK
Biomech Model Mechanobiol 5:180-91. 2006....
- Cell and nucleus deformation in compressed chondrocyte-alginate constructs: temporal changes and calculation of cell modulusM M Knight
Interdisciplinary Research Centre in Biomedical Materials and Department of Engineering, Queen Mary University of London, Mile End Rd, E1 4NS, London, UK
Biochim Biophys Acta 1570:1-8. 2002..This phenomenon enabled the calculation of a stress-strain relationship for the cell with an estimated Young's modulus value of approx. 3 kPa...
- Articular chondrocytes express connexin 43 hemichannels and P2 receptors - a putative mechanoreceptor complex involving the primary cilium?M M Knight
Queen Mary University of London, UK
J Anat 214:275-83. 2009..Furthermore, the expression of connexin 43 on the chondrocyte primary cilium suggests the possible involvement of the cilium in this pathway...
- Live cell imaging using confocal microscopy induces intracellular calcium transients and cell deathMartin M Knight
Interdisciplinary Research Centre, Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, United Kingdom
Am J Physiol Cell Physiol 284:C1083-9. 2003..Light-activated Ca2+ signaling may trigger alterations in numerous cell processes and thereby represent an important and hitherto overlooked artifact in fluorescent microscopy of viable cells...
- Mechanical compression and hydrostatic pressure induce reversible changes in actin cytoskeletal organisation in chondrocytes in agaroseM M Knight
IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary University of London, London, UK
J Biomech 39:1547-51. 2006..This reversible remodelling of actin cytoskeleton may therefore represent a mechanism through which the chondrocyte alters its mechanical properties and mechanosensitivity in response to physiological mechanical loading...
- Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strainDavid A Lee
IRC in Biomedical Materials and Medical Engineering Division, Department of Engineering, Queen Mary College, University of London, London, UK
Methods Mol Med 100:307-24. 2004..The chapter thus provides an introduction to the experimental techniques used to examine chondrocyte mechanotransduction and downstream cell function...
- Intracellular mechanics and mechanotransduction associated with chondrocyte deformation during pipette aspirationT Ohashi
Medical Engineering Division, Department of Engineering, Queen Mary University of London, London, UK
Biorheology 43:201-14. 2006..These findings have important implications for our understanding of cell mechanics and mechanotransduction as well as interpretation and modelling of pipette aspiration data...
- Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytesAngus K T Wann
School of Engineering and Materials Science, Queen Mary University of London, London, UK
FASEB J 26:1663-71. 2012....
- Cyclic loading opens hemichannels to release ATP as part of a chondrocyte mechanotransduction pathwayMariana Garcia
Queen Mary University of London, School of Engineering and Materials Science, Mile End Road, London E1 4NS, United Kingdom
J Orthop Res 28:510-5. 2010..In conclusion, mechanical loading triggers release of ATP via hemichannels. Hence, this study provides the first evidence of hemichannel involvement in chondrocyte mechanobiology...
- Viscoelastic cell mechanics and actin remodelling are dependent on the rate of applied pressurePriyanka Pravincumar
Institute of Bioengineering, School of Engineering and Material Science, Queen Mary University of London, London, United Kingdom
PLoS ONE 7:e43938. 2012..This study tested the hypothesis that the rate at which pressure (or stress) is applied influence the viscoelastic properties of the cell associated with differences in the dynamics of the actin cytoskeleton...
- Gap junction permeability between tenocytes within tendon fascicles is suppressed by tensile loadingEijiro Maeda
School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, E1 4NS, UK
Biomech Model Mechanobiol 11:439-47. 2012..This is therefore the first study to show that tenocyte gap junctions are not only important in transmitting mechanically activated signals but that mechanical loading directly regulates gap junction permeability...
- Confocal analysis of local and cellular strains in chondrocyte-agarose constructs subjected to mechanical shearYoshinori Sawae
Cell and Tissue Engineering Laboratories, Queen Mary University of London, London, United Kingdom
Ann Biomed Eng 32:860-70. 2004..This validated experimental system will enable future studies to examine the influence of shear on chondrocyte function and the associated mechanotransduction signalling pathways...
- Single photon counting fluorescence lifetime detection of pericellular oxygen concentrationsNeveen A Hosny
University of London, School of Engineering and Materials Science, Queen Mary, London, United Kingdom, E1 4NS
J Biomed Opt 17:016007. 2012..This technique provides a powerful tool for quantifying spatial oxygen gradients within three-dimensional cellular models...
- Biomechanical analysis of structural deformation in living cellsD L Bader
School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London, UK
Med Biol Eng Comput 46:951-63. 2008..The review focuses on deformation of chondrocytes with additional information on a variety of other cell types including neurons, red blood cells, epithelial cells and skin and muscle cells...
- Activation of chondrocytes calcium signalling by dynamic compression is independent of number of cyclesB Pingguan-Murphy
Medical Engineering Division, Department of Engineering and IRC in Biomedical Materials, Queen Mary University of London, London, UK
Arch Biochem Biophys 444:45-51. 2005..Therefore, Ca(2+) signalling may be part of a mechanotransduction pathway through which chondrocyte populations can modulate their metabolic activity in response to changing mechanical stimuli...
- Loading alters actin dynamics and up-regulates cofilin gene expression in chondrocytesJ J Campbell
Medical Engineering Division, School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK
Biochem Biophys Res Commun 361:329-34. 2007..Thus mechanical loading alters cortical actin dynamics, providing a potential mechanism through which chondrocytes can adapt their mechanical properties and mechanosensitivity to the local mechanical environment...
- Deformation properties of articular chondrocytes: a critique of three separate techniquesD L Bader
IRC in Biomedical Materials and Medical Engineering Division, Queen Mary, University of London, UK
Biorheology 39:69-78. 2002..7 kPa. The study highlights the difficulties in establishing unique mechanical parameters, which reflect the deformation behaviour of articular chondrocytes...
- The potential of pulsed low intensity ultrasound to stimulate chondrocytes matrix synthesis in agarose and monolayer culturesNatalie M Vaughan
School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK
Med Biol Eng Comput 48:1215-22. 2010..These results suggest that PLIUS has a limited potential to provide an effective method of stimulating matrix production as part of a tissue engineering strategy for cartilage repair...
- Both superficial and deep zone articular chondrocyte subpopulations exhibit the Crabtree effect but have different basal oxygen consumption ratesHannah K Heywood
School of Engineering and Materials Science, Queen Mary University of London, London, UK
J Cell Physiol 223:630-9. 2010..The work has further application to the optimisation of bioreactor design and engineered tissues...
- Stem cell mechanobiologyDavid A Lee
University of London, London E1 4NS, UK
J Cell Biochem 112:1-9. 2011....
- Purinergic pathway suppresses the release of .NO and stimulates proteoglycan synthesis in chondrocyte/agarose constructs subjected to dynamic compressionT T Chowdhury
Medical Engineering Division, Department of Engineering, Queen Mary, University of London, Mile End Road, London, UK
J Cell Physiol 209:845-53. 2006..The current study demonstrates for the first time the importance of a purinergic pathway in mediating the metabolic response to dynamic compression and suppressing an inflammatory effect...
- The effect of mechanical strain or hypoxia on cell death in subpopulations of rat dorsal root ganglion neurons in vitroS J Gladman
Centre for Neuroscience and Trauma, Blizard Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, E1 2AT, UK
Neuroscience 171:577-87. 2010..These results provide detailed information on the response of adult DRG subpopulations to hypoxia and mechanical strain, and describe in vitro models that could be useful for screening potential neuroprotective agents...
- Chondrocyte deformation within mechanically and enzymatically extracted chondrons compressed in agaroseM M Knight
Interdisciplinary Research Centre in Biomedical Materials, Queen Mary University of London, Mile End Rd, London E1 4NS, UK
Biochim Biophys Acta 1526:141-6. 2001..Although these differences may be reduced with time in culture, characterisation of mechanically isolated chondrons suggests that the stiffness of the chondrons in situ may be greater than previous estimates...
- Measurement of the deformation of isolated chondrocytes in agarose subjected to cyclic compressionM M Knight
IRC in Biomedical Materials, Queen Mary and Westfield College, London, UK
Med Eng Phys 20:684-8. 1998..72), although during static compression the cell deformation remained constant. This system may be used to examine cellular events under a range of dynamic mechanical stimuli...
- Eicosapentaenoic acid and docosahexaenoic acid reduce interleukin-1β-mediated cartilage degradationAngus K T Wann
School of Engineering and Materials Science, Queen Mary University of London, Mile End, London, E1 4NS, UK
Arthritis Res Ther 12:R207. 2010..The aim of this study was to examine the therapeutic potential of n-3 polyunsaturated fatty acids (PUFAs) in an in vitro model of cartilage inflammation...
- Osmotic challenge drives rapid and reversible chromatin condensation in chondrocytesJerome Irianto
Institute of Bioengineering, School of Engineering and Material Science, Queen Mary, University of London, London, United Kingdom Electronic address
Biophys J 104:759-69. 2013..Alterations in chromatin structure are expected to influence gene expression and thereby regulate chondrocyte activity in response to osmotic changes...
- Improved outcome after peripheral nerve injury in mice with increased levels of endogenous ω-3 polyunsaturated fatty acidsStacy J Gladman
Centre for Neuroscience and Trauma, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, E1 2AT, UK
J Neurosci 32:563-71. 2012..Fat-1 animals also had some protection against muscle atrophy after injury. In conclusion, both in vitro and in vivo experiments support the idea that a higher endogenous ω-3 PUFA could lead to beneficial effects after a PNI...
- An improved confocal FRAP technique for the measurement of long-term actin dynamics in individual stress fibersJ J Campbell
Department of Engineering, Medical Engineering Division, Queen Mary University of London, London E1 4NS, United Kingdom
Microsc Res Tech 70:1034-40. 2007..In conclusion, the present FRAP methodology overcomes many of the limitations of previous studies in order to provide new data describing long-term actin dynamics within individual stress fibers...
- Mitochondrial dynamics in chondrocytes and their connection to the mechanical properties of the cytoplasmZe ev Bomzon
Faculty of Civil and Environmental Engineering, Technion Israel Institute of Technology, Haifa 32000, Israel
J Biomech Eng 128:674-9. 2006..This paper presents a study that characterizes the diffusive motion of mitochondria in chondrocytes seeded in agarose constructs and what this implies about the mechanical properties of the cytoplasm...
- Ultrastructure of insect and spider cocoon silksOsnat Hakimi
Interdisciplinary Research Center in Biomedical Materials, Materials Department, and Center for Academic Surgery, Queen Mary University of London, London E1 4NS. o.hakimi@ qmul.ac.uk
Biomacromolecules 7:2901-8. 2006..Observed separation of the microfibrils in fractured brins suggests that the multifibrillar structure of the silk fiber contributes to toughness by allowing dissipation of energy in the controlled propagation of cracks...