F A Drobniewski
Affiliation: Queen Mary
- Rapid diagnostics of tuberculosis and drug resistance in the industrialized world: clinical and public health benefits and barriers to implementationFrancis Drobniewski
Public Health England National Mycobacterium Reference Laboratory, 2 Newark Street, London E1 2AT, UK
BMC Med 11:190. 2013..For example, whole genome sequencing of Mycobacterium tuberculosis offers a powerful new approach to the identification of drug resistance and to map transmission at a community and population level. ..
- Diagnosis of tuberculosis and drug resistance: what can new tools bring us?F Drobniewski
UK Health Protection Agency National Mycobacterium Reference Laboratory, London, UK
Int J Tuberc Lung Dis 16:860-70. 2012..Although great progress has been made, we are not there yet...
- A genome wide association study of pulmonary tuberculosis susceptibility in IndonesiansEileen Png
Human Genetics, Genome Institute of Singapore, 60 Biopolis Street, Singapore
BMC Med Genet 13:5. 2012..In order to identify additional targets in genetically dissimilar populations, and to enhance our understanding of this disease, we performed a multi-stage GWAS in a Southeast Asian cohort from Indonesia...
- Detection of resistance to second-line antituberculosis drugs by use of the genotype MTBDRsl assay: a multicenter evaluation and feasibility studyOlga Ignatyeva
Samara Oblast Tuberculosis Dispensary, Samara, Russia
J Clin Microbiol 50:1593-7. 2012..The sensitivity for the detection of kanamycin resistance needs improvement...
- The Directly Observed Therapy Short-Course (DOTS) strategy in Samara Oblast, Russian FederationY Balabanova
HPA Mycobacterium Reference Unit, Clinical TB and HIV Group, St Bartholomew and Queen Mary School of Medicine, London E1 2AT, UK
Respir Res 7:44. 2006....
- Analysis of undiagnosed tuberculosis-related deaths identified at post-mortem among HIV-infected patients in Russia: a descriptive studyYanina Balabanova
National Mycobacterium Reference Laboratory, Center for Infectious Diseases, Institute of Cell and Molecular Sciences, Queen Mary College, Barts and the London School of Medicine, University of London, 2 Newark Street, E1 2AT, London, UK
BMC Infect Dis 11:276. 2011....
- Clinical and ultrasonographic features of abdominal tuberculosis in HIV positive adults in ZambiaEdford Sinkala
Tropical Gastroenterology and Nutrition Group, University of Zambia School of Medicine, Lusaka, Zambia
BMC Infect Dis 9:44. 2009..There is little information on abdominal TB in HIV infection. We estimated the prevalence and clinical features of abdominal (excluding genitourinary) TB in HIV infected adults attending the University Teaching Hospital, Zambia...
- Use of Gamma-interferon assays in low- and medium-prevalence countries in Europe: a consensus statement of a Wolfheze Workshop organised by KNCV/EuroTB, Vilnius Sept 2006F Drobniewski
Mycobacterium Reference Unit and, Health Protection Agency, London, United Kingdom
Euro Surveill 12:E070726.2. 2007
- Performance of the Genotype(R) MTBDRPlus assay in the diagnosis of tuberculosis and drug resistance in Samara, Russian FederationVladyslav Nikolayevskyy
UK HPA Mycobacterium Reference Unit, Clinical TB and HIV Research Group, Institute of Cell and Molecular Science, Barts and the London School of Medicine, Queen Mary University, 2 Newark Street, London, E1 2AT, UK
BMC Clin Pathol 9:2. 2009..CONCLUSION: High sensitivity, short turnaround times and the potential for screening large numbers of specimens rapidly, make the GenoType(R) MTBDRplus assay suitable as a first-line screening assay for drug resistant TB...
- Rates of latent tuberculosis in health care staff in RussiaFrancis Drobniewski
Health Protection Agency Mycobacterium Reference Unit and Clinical TB and HIV Group, Institute of Cell and Molecular Sciences, Queen Mary College, University of London, London, United Kingdom
PLoS Med 4:e55. 2007..The objective of the study was to estimate the prevalence and determine the relative risks and risk factors for infection, within a vertically organised TB service in a country with universal bacille Calmette-Guérin (BCG) vaccination...
- Recommended standards for modern tuberculosis laboratory services in EuropeF A Drobniewski
HPA National Mycobacterium Reference Unit and Clinical TB and HIV Group, London, UK
Eur Respir J 28:903-9. 2006..Improved integration of laboratory medicine as a core part of all tuberculosis programmes is needed to achieve and maximise the potential of new developments...
- Antimicrobial susceptibility testing of Mycobacterium tuberculosis (EUCAST document E.DEF 8.1)--report of the Subcommittee on Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis of the European Committee for Antimicrobial Susceptibility TesF Drobniewski
Health Protection Agency National Mycobacterium Reference Unit, Centre for Infections, Institute for Cell and Molecular Sciences, London, UK
Clin Microbiol Infect 13:1144-56. 2007..There is a need to expand international external quality assessment to include second-line drug susceptibility testing...
- Typing Mycobacterium tuberculosis using variable number tandem repeat analysisT J Brown
HPA MRU, Queen Mary s School of Medicine and Dentistry, 2 Newark Street, London E1 2AT, UK
Methods Mol Biol 465:371-94. 2009..This chapter describes a high-throughput automated method for VNTR analysis at 15 loci using a capillary fragment analyzer and a manual method using agarose gel analysis...
- Comparison of three molecular assays for rapid detection of rifampin resistance in Mycobacterium tuberculosisS A Watterson
Public Health Laboratory Service Mycobacterium Reference Unit, Department of Microbiology, King s College School of Medicine and Dentistry, King s College Hospital Dulwich, London, United Kingdom
J Clin Microbiol 36:1969-73. 1998..7 and 100%), respectively. For the 21 isolates submitted by clinicians, both assays correlated 100% with routine testing...
- Rapid molecular detection of tuberculosis and rifampicin drug resistance: retrospective analysis of a national U.K. molecular service over the last decadeN Seoudi
Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University, London, UK
Thorax 67:361-7. 2012..Fast and reliable detection of Mycobacterium tuberculosis complex (MTBC) and drug resistance is crucial in establishing effective treatment and enforcing timely public health measures...
- Molecular epidemiology and prevalence of mutations conferring rifampicin and isoniazid resistance in Mycobacterium tuberculosis strains from the southern UkraineV V Nikolayevskyy
HPA Mycobacterium Reference Unit, Institute of Cell and Molecular Sciences, Clinical TB and HIV Group, Queen Mary s School of Medicine and Dentistry, London, UK
Clin Microbiol Infect 13:129-38. 2007..4% vs. 45.7%; RR 1.74; 95% CI 1.17-2.57), suggesting that this group may be responsible for rapid transmission of MDR TB in the southern Ukraine...
- The use of macroarrays for the identification of MDR Mycobacterium tuberculosisT J Brown
Health Protection Agency Mycobacterium Reference Unit, Kings College Hospital Dulwich, East Dulwich Grove, London SE22 8QF, UK
J Microbiol Methods 65:294-300. 2006..All mutations within katG and mabA-inhA were correctly identified. We conclude that this low cost, rapid system can usefully detect the mutations associated with the vast majority of MDR-Mtb...
- An integrated approach to rapid diagnosis of tuberculosis and multidrug resistance using liquid culture and molecular methods in RussiaYanina Balabanova
National Mycobacterium Reference Laboratory, Institute of Cell and Molecular Sciences, Queen Mary College, Barts and the London School of Medicine, University of London, London, United Kingdom
PLoS ONE 4:e7129. 2009..To analyse the feasibility, cost and performance of rapid tuberculosis (TB) molecular and culture systems, in a high multidrug-resistant TB (MDR TB) middle-income region (Samara, Russia) and provide evidence for WHO policy change...
- Mycobacterium tuberculosis and rifampin resistance, United KingdomI Ching Sam
Health Protection Agency, National Mycobacterium Reference Unit, Institute of Cell and Molecular Science, Barts and the London School of Medicine, 2 Newark Street, London, United Kingdom
Emerg Infect Dis 12:752-9. 2006..1%, 95.0%, 99.6%, 92.7%, and 99.7%, respectively. The assay enabled earlier diagnosis of MTBC and rifampin resistance (15.2 days) compared with culture-based techniques (30.7 days)...
- Human Mycobacterium bovis infections in London and Southeast EnglandM J Stone
National Mycobacterium Reference Laboratory, Health Protection Agency, London, United Kingdom
J Clin Microbiol 50:164-5. 2012..Low case numbers, despite a substantial animal reservoir, and resolution of all isolates provided no evidence of recent human-to-human transmission or recent significant infection from animals...
- Rifampin- and multidrug-resistant tuberculosis in Russian civilians and prison inmates: dominance of the beijing strain familyFrancis Drobniewski
Guy s King s amd St Thomas Medical School, King s College Hospital, London, United Kingdom
Emerg Infect Dis 8:1320-6. 2002..5%) were rifampin resistant. Rifampin-resistant Beijing isolates are dominant within the patient population, especially among prisoners, and threaten treatment programs...
- Molecular techniques in the diagnosis of Mycobacterium tuberculosis and the detection of drug resistanceM Caws
PHLS Mycobacterium Reference Unit, Guy s, King s and St Thomas School of Medicine, King s College Hospital Dulwich, London, UK
Ann N Y Acad Sci 953:138-45. 2001..Simple phenotypic methods for the detection of resistance to first-line drugs and genotypic kit-form assays for detection of rifampicin resistance have been developed that have become key tools in the containment of MDR TB...
- Antimicrobial prescribing patterns for respiratory diseases including tuberculosis in Russia: a possible role in drug resistance?Yanina Balabanova
KIL Consortium Sustainable TB Service Project, Mycobacterium Reference Unit, Department of Microbiology and Infection, Guy s King s and St Thomas Medical School, King s College, King s College Hospital Dulwich, East Dulwich Grove, London, UK
J Antimicrob Chemother 54:673-9. 2004..Inappropriate antibiotic prescribing exposes patients to the risk of side effects and encourages the development of drug resistance across antimicrobial groups used for respiratory infections including tuberculosis (TB)...
- Differentiation of tuberculosis strains in a population with mainly Beijing-family strainsVladyslav Nikolayevskyy
Barts and the London School of Medicine, University of London, London, United Kingdom
Emerg Infect Dis 12:1406-13. 2006..The expanded set of 25 MIRUs provided better discrimination than the original set of 15 (Hunter-Gaston diversity index 0.870 vs. 0.625). Loci MIRU 26, 1982, and 3232 were the most polymorphic in Beijing isolates...
- Rate of latent tuberculosis infection detected by occupational health screening of nurses new to a london teaching hospitalPriya Khanna
Department of Microbiology, Barts and The London National Health Service Trust, London, UK
Infect Control Hosp Epidemiol 30:581-4. 2009..On multivariate analysis, birth in a country with tuberculosis prevalence of more than 40 cases per 100,000 population was associated with positive results of both the IFN-gamma release assay and the tuberculin skin test...
- The 'bear trap': the colliding epidemics of tuberculosis and HIV in RussiaF A Drobniewski
Department of Infectious Diseases, Guy s King s and St Thomas Medical School, East Dulwich Grove, London, UK
Int J STD AIDS 15:641-6. 2004..Insights gained through the examination of this one region can tell us something of the magnitude of the challenge now faced by both the international community and Russia...
- Specificity of IS6110-based DNA fingerprinting and diagnostic techniques for Mycobacterium tuberculosis complexW A Githui
PHLS Mycobacterium Reference Unit, Dulwich Public Health Laboratory and Department of Microbiology, Kings College School of Medicine and Dentistry, Dulwich Hospital, London, United Kingdom
J Clin Microbiol 37:1224-6. 1999..tuberculosis complex. In addition, DNA amplification with IS6110-specific primers yielded a 181-bp fragment only in DNA from M. tuberculosis complex isolates...
- Tuberculosis and the role of war in the modern eraF A Drobniewski
Dulwich Public Health Laboratory, Guy s King s and St Thomas Medical School, King s College Hospital Dulwich, London, UK
Int J Tuberc Lung Dis 4:1120-5. 2000..Tuberculosis (TB) remains a major global health problem; historically, major wars have increased TB notifications. This study evaluated whether modern conflicts worldwide affected TB notifications between 1975 and 1995...
- Management of multiple drug-resistant tuberculosisD C S Hutchison
Department of Respiratory Medicine, King s College Hospital, London, UK
Respir Med 97:65-70. 2003..The prognosis for patients treated at specialised centres is good (and better than is generally believed). We describe a new protocol for the detection and management of MDR-TB...
- High coverage with HAART is required to substantially reduce the number of deaths from tuberculosis: system dynamics simulationRifat A Atun
Centre for Health Management, Tanaka Business School, Imperial College London, South Kensington Campus, London, UK
Int J STD AIDS 18:267-73. 2007..High coverage with HAART is required to substantially reduce the number of deaths from tuberculosis...
- Increasing antituberculosis drug resistance in the United Kingdom: analysis of National Surveillance DataMichelle E Kruijshaar
Respiratory Diseases Department, Centre for Infections, Health Protection Agency, London NW9 5EQ
BMJ 336:1231-4. 2008..To identify recent trends in, and factors associated with, resistance to antituberculosis drugs in England, Wales, and Northern Ireland...
- High rates of clustering of strains causing tuberculosis in Harare, Zimbabwe: a molecular epidemiological studyPhilippa J Easterbrook
Department of HIV GU Medicine, The Guy s, King s and St Thomas s School of Medicine, King s College Hospital, Denmark Hill Campus, London, United Kingdom
J Clin Microbiol 42:4536-44. 2004..Geographic linkage could be detected in a proportion of these clusters. A small group of actively circulating strains accounted for most of the cases of TB transmission...
- Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by INNO-LiPA Rif.TB assay: retrospective analyses of Health Protection Agency National Mycobacterium Reference Laboratory dataSrinivasulu Reddy
Health Protection Agency National Mycobacterium Reference Laboratory, Institute of Cell and Molecular Science, 2 Newark Street, London E1 2AT, UK
J Med Microbiol 59:563-6. 2010..Referring clinicians should be aware of the limitations of this assay on PETs, and should request molecular testing only in patients with a high clinical probability of TB and when acid-fast bacilli are seen in tissue specimens...
- Evaluation of two molecular assays for rapid detection of mycobacterium tuberculosis resistance to fluoroquinolones in high-tuberculosis and -multidrug-resistance SettingsI Kontsevaya
Health Protection Agency National Mycobacterium Reference Laboratory and Clinical and HIV Group, Institute for Cell and Molecular Sciences, Barts and the London Medical School, Queen Mary, University of London, 2 Newark Street, London E12AT, United Kingdom
J Clin Microbiol 49:2832-7. 2011..9% of the analyzed strains were unreadable when MTBDRsl was used. The MTBDRsl and pyrosequencing assays offer a rapid and accurate means for diagnosing resistance to FQs in high-TB-burden areas...
- Clinical features, diagnosis, and management of multiple drug-resistant tuberculosis since 2002Francis Drobniewski
Mycobacterium Reference Unit, Department of Microbiology and Infection, Guy s, King s, and St Thomas Medical School, King s College Hospital Dulwich, London, UK
Curr Opin Pulm Med 10:211-7. 2004..Multiple drug-resistant tuberculosis is increasing globally, particularly in Eastern Europe. This review summarizes advances in our understanding of the epidemiology, diagnosis, and treatment of MDRTB in 2002-2003...
- Discriminatory ability of hypervariable variable number tandem repeat loci in population-based analysis of Mycobacterium tuberculosis strains, London, UKPreya Velji
Barts and the London School of Medicine, Queen Mary, University of London, London, UK
Emerg Infect Dis 15:1609-16. 2009..909. Hypervariable loci should be included in standardized panels because they can provide consistent comparable results at multiple settings, provided the proposed conditions are adhered to...
- Comparison of interferon-gamma release assays and tuberculin skin test in predicting active tuberculosis (TB) in children in the UK: a paediatric TB network studyAlasdair R J Bamford
Academic Department of Paediatrics, Imperial College London, London, UK
Arch Dis Child 95:180-6. 2010..The authors examined the sensitivity of commercially available IGRA to diagnose active TB in children in the UK compared with the tuberculin skin test (TST)...
- Impact of an effective multidrug-resistant tuberculosis control programme in the setting of an immature HIV epidemic: system dynamics simulation modelRifat A Atun
Centre for Health Management, The Business School, Imperial College London, South Kensington Campus, London, UK
Int J STD AIDS 16:560-70. 2005..The epidemiological starting point for these scenarios is present in many regions within the former Soviet Union and this analysis suggests control of MDRTB should be an urgent priority...
- Can 15-locus mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis provide insight into the evolution of Mycobacterium tuberculosis?Andrea Gibson
Mycobacterium Reference Unit, Health Protection Agency, Barts and The London Queen Mary s School of Medicine and Dentistry, London, United Kingdom
Appl Environ Microbiol 71:8207-13. 2005..MIRU-VNTR is a valuable tool for phylogenetic studies and could define an evolutionarily uncharacterized population of M. tuberculosis complex organisms...
- Tuberculosis transmission attributable to close contacts and HIV status, MalawiAmelia C Crampin
London School of Hygiene and Tropical Medicine, Keppel Street, London, United Kingdom
Emerg Infect Dis 12:729-35. 2006..We estimate that 9%-13% of TB cases were attributable to recent transmission from identifiable close contacts and that nearly half of the TB cases arising from recent infection had acquired the infection from HIV-positive patients...
- Silent nucleotide polymorphisms and a phylogeny for Mycobacterium tuberculosisLucy Baker
Health Protection Agency, London, United Kingdom
Emerg Infect Dis 10:1568-77. 2004..tuberculosis strains and provides a unifying framework for both epidemiologic and evolutionary analysis of M. tuberculosis populations...
- Drug-resistant tuberculosis, clinical virulence, and the dominance of the Beijing strain family in RussiaFrancis Drobniewski
HPA Mycobacterium Reference Unit, Department of Microbiology and Infection, Guy s King s and St Thomas Medical School, London, England
JAMA 293:2726-31. 2005..Tuberculosis and multidrug-resistant tuberculosis is a serious public health problem in Russia...
- Health-systems efficiency in the Russian Federation: tuberculosis controlKatherine Floyd
Stop TB Department, World Health Organization, Geneva, Switzerland
Bull World Health Organ 84:43-51. 2006..We also sought to quantify the extent to which efficiency could be improved by reducing hospitalization rates and re-profiling hospital beds available in the tuberculosis-control system...
- Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110Jeremy W Dale
School of Biomedical and Life Sciences, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom
J Bacteriol 185:2555-62. 2003..These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110...
- The importance of recent infection with Mycobacterium tuberculosis in an area with high HIV prevalence: a long-term molecular epidemiological study in Northern MalawiJudith R Glynn
Department of Infectious Diseases, London School of Hygiene and Tropical Medicine, UK
J Infect Dis 192:480-7. 2005..Here, we present what is, to our knowledge, the first such study in an area with high human immunodeficiency virus (HIV) prevalence...
- Implementing WHO DOTS strategy in the Russian Federation: stakeholder attitudesRifat A Atun
Centre for Health Management, Tanaka Business School, Imperial College London, South Kensington Campus, London SW7 2PG, UK
Health Policy 74:122-32. 2005..Cultural issues and capacity constraints, especially in laboratory equipment and personnel, would impede introduction and sustainability of the DOTS strategy...
- Worldwide emergence of extensively drug-resistant tuberculosisN Sarita Shah
Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Emerg Infect Dis 13:380-7. 2007..9%) met criteria for XDR TB. Further investigation of population-based trends and expanded efforts to prevent drug resistance and effectively treat patients with MDR TB are crucial for protection of public health and control of TB...
- Analysis of association of the TIRAP (MAL) S180L variant and tuberculosis in three populationsSergey Nejentsev
Nat Genet 40:261-2; author reply 262-3. 2008
- Analysis of how the health systems context shapes responses to the control of human immunodeficiency virus: case-studies from the Russian FederationRifat A Atun
Centre for Health Management, Tanaka Business School, Imperial College London, South Kensington Campus, London SW7 2AZ, England, UK
Bull World Health Organ 83:730-8. 2005..To develop a methodology and an instrument that allow the simultaneous rapid and systematic examination of the broad public health context, the health care systems, and the features of disease-specific programmes...
- Risk factors for pulmonary tuberculosis in Russia: case-control studyRichard Coker
European Centre on Health of Societies in Transition, Department of Public Health and Policy, London School of Hygiene and Tropical Medicine, London WC1E 7HT
BMJ 332:85-7. 2006..To determine risk factors for pulmonary tuberculosis in Russia...
- Global distribution of Mycobacterium tuberculosis spoligotypesIngrid Filliol
Unite de la Tuberculose et des Mycobacteries, Institut Pasteur, Pointe a Pitre, Guadeloupe, French West Indies
Emerg Infect Dis 8:1347-9. 2002..The 11708 spoligotypes were clustered into 813 shared types. A total of 1300 orphan patterns (clinical isolates showing a unique spoligotype) were also detected...
- Medical and social analysis of prisoners with tuberculosis in a Russian prison colony: an observational studyFrancis Drobniewski
Clin Infect Dis 36:234-5. 2003
- Origins and properties of Mycobacterium tuberculosis isolates in LondonJeremy W Dale
School of Biomedical and Molecular Sciences, University of Surrey, Guildford GU2 7XH, UK
J Med Microbiol 54:575-82. 2005....
- Snapshot of moving and expanding clones of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an international studyIngrid Filliol
Unite de la Tuberculose et des Mycobacteries, Institut Pasteur de Guadeloupe, Pointe a Pitre, Guadeloupe
J Clin Microbiol 41:1963-70. 2003..Intercontinental matches of shared types among eight continents and subcontinents (Africa, North America, Central America, South America, Europe, the Middle East and Central Asia, and the Far East) are analyzed and discussed...
- Resequencing and association analysis of the SP110 gene in adult pulmonary tuberculosisJeffrey S Szeszko
Juvenile Diabetes Research Foundation Wellcome Trust Diabetes and Inflammation Laboratory, Cambridge Institute for Medical Research, Department of Medical Genetics, University of Cambridge, WT MRC building, Addenbrooke s Hospital, Cambridge, CB2 2XY, UK
Hum Genet 121:155-60. 2007..Our results indicate that common polymorphisms of the SP110 gene have no major effect on susceptibility to tuberculosis in this population...
- Mycobacterium bovis infection, United KingdomRobert M M Smith
Communicable Disease Surveillance Centre, National Public Health Service for Wales, Abton House, Wedal Road, Cardiff CF14 3QX, Wales, United Kingdom
Emerg Infect Dis 10:539-41. 2004..This finding suggests that there may be a small risk for transmission to humans, making continued vigilance particularly necessary...