S A Bustin

Summary

Affiliation: Queen Mary
Country: UK

Publications

  1. pmc Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
    Stephen A Bustin
    Centre for Academic Surgery Institute of Cell and Molecular Science Barts, University of London, UK
    J Biomol Tech 15:155-66. 2004
  2. ncbi request reprint Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences
    Stephen A Bustin
    The Royal London Hospital, Centre for Academic Surgery, 4th Floor Alexandra Wing, London, E1 1BB, UK
    Expert Rev Mol Diagn 5:493-8. 2005
  3. doi request reprint The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
    Stephen A Bustin
    Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK
    Clin Chem 55:611-22. 2009
  4. doi request reprint Molecular staging of colorectal cancer: new paradigm or waste of time?
    Jamie Murphy B
    Centre for Academic Surgery Barts and the London Queen Mary s School for Medicine and Dentistry, 3rd Floor Alexandra Wing, Royal London Hospital, London E1 1BB, UK Tel 44 20 7377 7000 extn 2431
    Expert Opin Med Diagn 1:31-45. 2007
  5. pmc Colorectal cancers with microsatellite instability display mRNA expression signatures characteristic of increased immunogenicity
    Ayan Banerjea
    Centre for Academic Surgery, Barts and the London Queen Mary School of Medicine and Dentistry, The Royal London Hospital, Whitechapel, London, E1 1BB, UK
    Mol Cancer 3:21. 2004
  6. ncbi request reprint Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
    S A Bustin
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, UK
    J Mol Endocrinol 29:23-39. 2002
  7. ncbi request reprint Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase C mRNA levels in lymph nodes may not predict treatment failure in colorectal cancer patients
    Stephen A Bustin
    Centre for Academic Surgery, Barts and The London Queen Mary s School of Medicine and Dentistry, London, United Kingdom
    Int J Cancer 108:412-7. 2004
  8. ncbi request reprint Tenth annual nucleic acid-based technologies: time to stop and think. Washington, DC, USA, 24-26 June, 2002
    Stephen A Bustin
    Queen Mary s School of Medicine, Academic Department of Surgery, University of London, London, E1 1BB, UK
    Expert Rev Mol Diagn 2:405-8. 2002
  9. ncbi request reprint Gene expression profiling for molecular staging and prognosis prediction in colorectal cancer
    Stephen A Bustin
    Royal London Hospital, London E1 1BB, UK
    Expert Rev Mol Diagn 4:599-607. 2004
  10. ncbi request reprint Quantitative real-time RT-PCR--a perspective
    S A Bustin
    Institute of Cellular and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK
    J Mol Endocrinol 34:597-601. 2005

Collaborators

Detail Information

Publications50

  1. pmc Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
    Stephen A Bustin
    Centre for Academic Surgery Institute of Cell and Molecular Science Barts, University of London, UK
    J Biomol Tech 15:155-66. 2004
    ....
  2. ncbi request reprint Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences
    Stephen A Bustin
    The Royal London Hospital, Centre for Academic Surgery, 4th Floor Alexandra Wing, London, E1 1BB, UK
    Expert Rev Mol Diagn 5:493-8. 2005
    ..These results emphasize the urgent need for the establishment of best practice guidelines for this technology, particularly in the context of its mounting adaptation as a high-throughput clinical diagnostic assay...
  3. doi request reprint The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
    Stephen A Bustin
    Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK
    Clin Chem 55:611-22. 2009
    ..The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments...
  4. doi request reprint Molecular staging of colorectal cancer: new paradigm or waste of time?
    Jamie Murphy B
    Centre for Academic Surgery Barts and the London Queen Mary s School for Medicine and Dentistry, 3rd Floor Alexandra Wing, Royal London Hospital, London E1 1BB, UK Tel 44 20 7377 7000 extn 2431
    Expert Opin Med Diagn 1:31-45. 2007
    ..Only then can the implementation of molecular staging assays into clinical practice be considered...
  5. pmc Colorectal cancers with microsatellite instability display mRNA expression signatures characteristic of increased immunogenicity
    Ayan Banerjea
    Centre for Academic Surgery, Barts and the London Queen Mary School of Medicine and Dentistry, The Royal London Hospital, Whitechapel, London, E1 1BB, UK
    Mol Cancer 3:21. 2004
    ..We aimed to compare the gene expression profiles of MSI-H and MSS cancers to provide evidence for an activated immune response in the former...
  6. ncbi request reprint Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
    S A Bustin
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, UK
    J Mol Endocrinol 29:23-39. 2002
    ....
  7. ncbi request reprint Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase C mRNA levels in lymph nodes may not predict treatment failure in colorectal cancer patients
    Stephen A Bustin
    Centre for Academic Surgery, Barts and The London Queen Mary s School of Medicine and Dentistry, London, United Kingdom
    Int J Cancer 108:412-7. 2004
    ..Instead, accurate prognostic stratification requires careful assessment of the likely metastatic potential of the primary cancer...
  8. ncbi request reprint Tenth annual nucleic acid-based technologies: time to stop and think. Washington, DC, USA, 24-26 June, 2002
    Stephen A Bustin
    Queen Mary s School of Medicine, Academic Department of Surgery, University of London, London, E1 1BB, UK
    Expert Rev Mol Diagn 2:405-8. 2002
  9. ncbi request reprint Gene expression profiling for molecular staging and prognosis prediction in colorectal cancer
    Stephen A Bustin
    Royal London Hospital, London E1 1BB, UK
    Expert Rev Mol Diagn 4:599-607. 2004
    ..In turn, this may allow a clinically relevant definition of patient subgroups based on individual molecular parameters for rational decision making regarding choice of therapy...
  10. ncbi request reprint Quantitative real-time RT-PCR--a perspective
    S A Bustin
    Institute of Cellular and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK
    J Mol Endocrinol 34:597-601. 2005
    ....
  11. ncbi request reprint The value of microarray techniques for quantitative gene profiling in molecular diagnostics
    Stephen A Bustin
    Academic Dept of Surgery, Barts, London Queen Mary s School of Medicine and Dentistry, University of London, London, UK, E1 1BB
    Trends Mol Med 8:269-72. 2002
    ..Therefore, numerous issues related to format, quality, validation and interpretation remain to be resolved before microarray profiling can become a diagnostic tool of clinical relevance for routine work...
  12. ncbi request reprint Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
    Stephen A Bustin
    Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, U K
    Clin Sci (Lond) 109:365-79. 2005
    ....
  13. ncbi request reprint Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer
    Stephen A Bustin
    Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, UK
    Mol Aspects Med 27:192-223. 2006
    ....
  14. doi request reprint In silico tools for qPCR assay design and data analysis
    Stephen Bustin
    Centre for Digestive Diseases, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, The Royal London Hospital, E1 1BB, London, UK
    Methods Mol Biol 760:283-306. 2011
    ..Some of the packages available for validation of the resulting Cq data and detailed statistical analysis are described...
  15. doi request reprint Developments in real-time PCR research and molecular diagnostics
    Stephen A Bustin
    Blizard Institute for Cell and Molecular Science, Bart s and The London School of Medicine and Dentistry, University of London, London, UK and 3rd Floor Alexandra Wing, Royal London Hospital, London, UK
    Expert Rev Mol Diagn 10:713-5. 2010
    ..It provided a fascinating snapshot of current trends and novel approaches, as well as important issues concerning assay design, optimization and quality control issues...
  16. doi request reprint Why the need for qPCR publication guidelines?--The case for MIQE
    Stephen A Bustin
    Blizard Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Whitechapel, London E1 1BB, UK
    Methods 50:217-26. 2010
    ..General implementation of these guidelines is an important requisite for the maturing of qPCR into a robust, accurate and reliable nucleic acid quantification technology...
  17. ncbi request reprint Molecular quantification and mapping of lymph-node micrometastases in cervical cancer
    P O Van Trappen
    Academic Department of Gynaecological Oncology, Queen Mary and Westfield College, St Bartholomew s Hospital, London, UK
    Lancet 357:15-20. 2001
    ..Reverse-transcriptase PCR (RT-PCR) is a highly sensitive method for detection of lymph-node micrometastases, but accurate quantitative assessment has been difficult...
  18. ncbi request reprint Expression of the Ca2+-activated chloride channel genes CLCA1 and CLCA2 is downregulated in human colorectal cancer
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
    DNA Cell Biol 20:331-8. 2001
    ..Our results suggest that CLCA1 could specify a new tumor suppressor and that, as in breast cancer, CLCA2 may function as a tumor suppressor in colorectal cancer...
  19. ncbi request reprint Cytokeratin 20 is not a tissue-specific marker for the detection of malignant epithelial cells in the blood of colorectal cancer patients
    S A Bustin
    Academic Department of Surgery, St Bartholomew s, Royal London School of Medicine and Dentistry, London, England
    Int J Surg Investig 2:49-57. 2000
    ..Cytokeratin 20 has been proposed as a tissue-specific marker for the detection of micrometastases in the blood of colorectal cancer patients. However, recent reports have challenged its specificity, and hence the validity of its use...
  20. ncbi request reprint Expression of HLA class II in colorectal cancer: evidence for enhanced immunogenicity of microsatellite-instability-positive tumours
    S A Bustin
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, London, UK
    Tumour Biol 22:294-8. 2001
    ....
  21. ncbi request reprint Molecular assessment of tumour stage and disease recurrence using PCR-based assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s, London, UK
    Mol Med Today 4:389-96. 1998
    ..Its role in the identification of minimal residual disease after curative surgical resection requires clinical validation in further prospective studies...
  22. pmc Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, UK
    Br J Cancer 79:1813-20. 1999
    ..We conclude that neither ck19 nor ck20 are reliable markers for the detection of colon epithelial cells in peripheral blood and that an evaluation of the usefulness of GCC awaits further longitudinal studies...
  23. ncbi request reprint Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London E1 1BB, UK
    J Mol Endocrinol 25:169-93. 2000
    ..It illustrates the usefulness of these assays by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH)...
  24. ncbi request reprint Tumour-infiltrating lymphocytes in colorectal cancer with microsatellite instability are activated and cytotoxic
    S M Phillips
    Academic Department of Surgery, Royal London Hospital, London, UK
    Br J Surg 91:469-75. 2004
    ..The nature of tumour-infiltrating lymphocytes in colorectal cancers was investigated using quantitative real-time polymerase chain reaction (PCR) and immunohistochemistry...
  25. ncbi request reprint Increased levels of insulin-like growth factor binding protein-2 in sera and tumours from patients with colonic neoplasia with and without acromegaly
    F Miraki-Moud
    Department of Endocrinology, St. Bartholomew's Hospital, London, UK
    Clin Endocrinol (Oxf) 54:499-508. 2001
    ....
  26. ncbi request reprint The growth hormone-insulin-like growth factor-I axis and colorectal cancer
    S A Bustin
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK E1 1BB
    Trends Mol Med 7:447-54. 2001
    ..However, the molecular mechanisms and signalling pathways responsible are only beginning to be unravelled, as are the relative contributions of the endocrine and autocrine or paracrine effects...
  27. ncbi request reprint Differential expression of IGF-binding protein-3 in normal and malignant colon and its influence on apoptosis
    P J Jenkins
    Department of Endocrinology, William Harvey Research Institute, Barts and the London Queen Mary s School of Dentistry, John Vane Building, Charterhouse Square, UK
    Endocr Relat Cancer 12:891-901. 2005
    ..These effects are mediated in part by the PI-3K pathway in contrast to the MAP kinase pathway used by IGF-I...
  28. ncbi request reprint Immunogenic hsp-70 is overexpressed in colorectal cancers with high-degree microsatellite instability
    Ayan Banerjea
    Centre for Academic Surgery, Barts and the London Queen Mary School of Medicine and Dentistry, The Royal London Hospital, Whitechapel, London, United Kingdom
    Dis Colon Rectum 48:2322-8. 2005
    ..We aimed to explore the expression of heat shock proteins 70 and 110 and their relationship to microsatellite instability, survival, and other clinicopathologic parameters...
  29. ncbi request reprint Extended silencing of the insulin-like growth factor-I receptor gene in MCF-7 cells by serial transfection with siRNA
    Clare McVittie
    Department of Breast Surgery, St Bartholomew s and The London Hospitals, London EC1A 7BE, UK
    Anal Biochem 357:305-7. 2006
  30. doi request reprint Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?
    Jamie Murphy
    Centre for Academic Surgery, Royal London Hospital, London, UK
    Expert Rev Mol Diagn 9:187-97. 2009
    ..This serves as a timely reminder of the indispensable requirement for careful experimental design, validation and analysis...
  31. ncbi request reprint Evidence for a link between IGF-I and cancer
    Paul J Jenkins
    Department of Endocrinology, St Bartholomew s Hospital, London EC1A 7BE, UK
    Eur J Endocrinol 151:S17-22. 2004
    ..The increased understanding of the molecular mechanisms and signalling pathways regulated by the GH/IGF-I axis has started to provide significant insights into the aetiology, prevention and therapy for a number of common cancers...
  32. ncbi request reprint Age and diet act through distinct isoforms of the class II transactivator gene in mouse intestinal epithelium
    Ian R Sanderson
    Research Centre for Gastroenterology, and Research Centre for Surgery, Institute of Cell and Molecular Science, Barts, England
    Gastroenterology 127:203-12. 2004
    ..Because the class II transactivator protein (CIITA) induces Ia and Ii in most cell types, we hypothesized that diet-induced expression of these genes was through CIITA...
  33. ncbi request reprint Differential expression patterns of the insulin-like growth factor 2 gene in human colorectal cancer
    Shu Rui Li
    Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, London, UK
    Tumour Biol 25:62-8. 2004
    ..Also, individuals with loss of genomic imprinting (LOI) causing over-expression of IGF 2 may be at greater risk of developing CRC. However, this LOI may be reversed in MSI-H patients...
  34. ncbi request reprint Colorectal cancers with mononucleotide microsatellite instability can be identified using microfabricated chip technology
    Ayan Banerjea
    Academic Department of Surgery, Barts and The London Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, UK
    Anal Biochem 322:130-3. 2003
  35. ncbi request reprint Identification of differentially expressed genes associated with colorectal cancer liver metastasis
    S R Li
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
    Eur Surg Res 35:327-36. 2003
    ....
  36. pmc Altered monocyte cyclo-oxygenase response in non-obese diabetic mice
    H Beyan
    Centre for Diabetes and Metabolic Medicine DMM, Institute of Cell and Molecular Science, London, UK
    Clin Exp Immunol 155:304-10. 2009
    ..However, Vioxx failed to affect insulitis or diabetes. We conclude that monocyte responses are altered in diabetic NOD mice but COX-2 expression is unlikely to be critical to disease risk...
  37. ncbi request reprint Local expression of insulin-like growth factor-I affects angiogenesis in colorectal cancer
    Stephen A Bustin
    Academic Department of Surgery, Barts and The London Queen Mary s School of Medicine and Dentistry, London, UK
    Tumour Biol 23:130-8. 2002
    ..The biological relevance of this finding is suggested by the significant association between local IGF-I mRNA levels and microvessel density in the colorectal cancers...
  38. ncbi request reprint Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase mRNA in individuals with colorectal cancer
    B W Ogunkolade
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, UK
    Lancet 359:1831-2. 2002
    ....
  39. ncbi request reprint Validation of housekeeping genes for normalizing RNA expression in real-time PCR
    Keertan Dheda
    Royal Free Medical School, London, UK
    Biotechniques 37:112-4, 116, 118-9. 2004
    ..Validations of housekeeping genes are highly specific for a particular experimental model and are a crucial component in assessing any new model...
  40. ncbi request reprint Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians
    Babatunji William Ogunkolade
    Department of Diabetes and Metabolic Medicine, Barts and The London Queen Mary s School of Medicine and Dentistry, University of London, Royal London Hospital, Whitechapel, London E1 1BB, UK
    Diabetes 51:2294-300. 2002
    ..Furthermore, VDR expression has been shown to be a determinant of insulin secretory capacity...
  41. ncbi request reprint Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase, and vitamin D receptor mRNA in normal and malignant breast tissue
    Kathryn McCarthy
    Section of Endocrine Oncology, Department of Endocrinology, Barts and The London, Queen Mary School of Medicine, St Bartholomew s Hospital, London EC1A 6BT, UK
    Anticancer Res 29:155-7. 2009
    ..The decreased expression of 1alphaOHase in normal tissue from women with breast cancer may be important in their predisposition to the development of cancer...
  42. ncbi request reprint Expression of PRPF31 mRNA in patients with autosomal dominant retinitis pigmentosa: a molecular clue for incomplete penetrance?
    Eranga N Vithana
    Division of Molecular Genetics, Institute of Ophthalmology, University College London, London, UK
    Invest Ophthalmol Vis Sci 44:4204-9. 2003
    ..4 (RP11) with mutations in the PRPF31 gene is due to differentially expressed wild-type alleles in symptomatic and asymptomatic individuals...
  43. ncbi request reprint Vitamin D metabolism in peripheral blood mononuclear cells is influenced by chewing "betel nut" (Areca catechu) and vitamin D status
    William B Ogunkolade
    Center for Diabetes and Metabolic Medicine, Royal London Hospital, London E1 1BB, United Kingdom
    J Clin Endocrinol Metab 91:2612-7. 2006
    ..We previously reported that circulating 1,25(OH)(2)D contributed to determination of VDR-protein levels and VDR genotype was a determinant of both VDR mRNA and VDR-protein in South Asians...
  44. ncbi request reprint Quantification of mRNA using real-time RT-PCR
    Tania Nolan
    Sigma Aldrich, Homefield Road, Haverhill, UK
    Nat Protoc 1:1559-82. 2006
    ..The entire protocol described here, encompassing all stages from initial assay design to reliable qPCR data analysis, requires approximately 15 h...
  45. ncbi request reprint Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    Carmela Tricarico
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy
    Anal Biochem 309:293-300. 2002
    ..Our results suggest that the use of internal standards comprising single housekeeping genes or rRNA is inappropriate for studies involving tissue biopsies...
  46. ncbi request reprint The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans
    Sharmilee Gnanapavan
    Department of Endocrinology, St Bartholomew s and The Royal London Hospital, London ECIA 7BE, UK
    J Clin Endocrinol Metab 87:2988. 2002
    ..These data suggest that ghrelin might have widespread physiological effects via different, partly unidentified, subtypes of the GHS-R in endocrine and non-endocrine tissues...
  47. pmc Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte
    Radek Sindelka
    Laboratory of Gene Expression, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic
    Nucleic Acids Res 36:387-92. 2008
    ..mRNA expression profiles did not change following in vitro fertilization and we conclude that the cortical rotation that follows fertilization has no detectable effect on intracellular mRNA gradients...
  48. ncbi request reprint Altered monocyte cyclooxygenase response to lipopolysaccharide in type 1 diabetes
    Huriya Beyan
    Department of Diabetes and Metabolic Medicine, Institute of Cell and Molecular Science, London E1 2AT, U K
    Diabetes 55:3439-45. 2006
    ..Because COX-2 response to LPS is proinflammatory, an inherited reduced response would predispose to chronic inflammatory diseases such as type 1 diabetes...
  49. ncbi request reprint The GH-IGF-I axis and breast cancer
    Christiana Laban
    Department of Breast Surgery, St Bartholomew s Hospital, West Smithfield, London EC1A 7BE, UK
    Trends Endocrinol Metab 14:28-34. 2003
    ..Here, we discuss the influence of the GH-IGF-I axis in normal mammary development and homeostasis, its putative role in breast tumorigenesis and its interactions with estrogen signalling...
  50. ncbi request reprint SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
    Tania Nolan
    Sigma Aldrich, Haverhill CB9 8QP, UK
    Anal Biochem 351:308-10. 2006