Research Topics
| S A BustinSummaryAffiliation: Queen Mary Country: UK Publications
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Publications
Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferencesStephen A Bustin
The Royal London Hospital, Centre for Academic Surgery, 4th Floor Alexandra Wing, London, E1 1BB, UK
Expert Rev Mol Diagn 5:493-8. 2005..These results emphasize the urgent need for the establishment of best practice guidelines for this technology, particularly in the context of its mounting adaptation as a high-throughput clinical diagnostic assay...- Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsiesCarmela Tricarico
Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy
Anal Biochem 309:293-300. 2002..Our results suggest that the use of internal standards comprising single housekeeping genes or rRNA is inappropriate for studies involving tissue biopsies... - Cytokeratin 20 is not a tissue-specific marker for the detection of malignant epithelial cells in the blood of colorectal cancer patientsS A Bustin
Academic Department of Surgery, St Bartholomew s, Royal London School of Medicine and Dentistry, London, England
Int J Surg Investig 2:49-57. 2000..Cytokeratin 20 has been proposed as a tissue-specific marker for the detection of micrometastases in the blood of colorectal cancer patients. However, recent reports have challenged its specificity, and hence the validity of its use... - The growth hormone-insulin-like growth factor-I axis and colorectal cancerS A Bustin
Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK E1 1BB
Trends Mol Med 7:447-54. 2001..However, the molecular mechanisms and signalling pathways responsible are only beginning to be unravelled, as are the relative contributions of the endocrine and autocrine or paracrine effects... - Expression of HLA class II in colorectal cancer: evidence for enhanced immunogenicity of microsatellite-instability-positive tumoursS A Bustin
Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, London, UK
Tumour Biol 22:294-8. 2001.... - Expression of the Ca2+-activated chloride channel genes CLCA1 and CLCA2 is downregulated in human colorectal cancerS A Bustin
Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
DNA Cell Biol 20:331-8. 2001..Our results suggest that CLCA1 could specify a new tumor suppressor and that, as in breast cancer, CLCA2 may function as a tumor suppressor in colorectal cancer... - Local expression of insulin-like growth factor-I affects angiogenesis in colorectal cancerStephen A Bustin
Academic Department of Surgery, Barts and The London Queen Mary s School of Medicine and Dentistry, London, UK
Tumour Biol 23:130-8. 2002..The biological relevance of this finding is suggested by the significant association between local IGF-I mRNA levels and microvessel density in the colorectal cancers... - Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysS A Bustin
Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London E1 1BB, UK
J Mol Endocrinol 25:169-93. 2000..It illustrates the usefulness of these assays by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH)... - The product of the primary response gene BRF1 inhibits the interaction between 14-3-3 proteins and cRaf-1 in the yeast trihybrid systemS A Bustin
Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, England
DNA Cell Biol 18:653-61. 1999.... - Molecular assessment of tumour stage and disease recurrence using PCR-based assaysS A Bustin
Academic Department of Surgery, St Bartholomew s, London, UK
Mol Med Today 4:389-96. 1998..Its role in the identification of minimal residual disease after curative surgical resection requires clinical validation in further prospective studies... - Increased levels of insulin-like growth factor binding protein-2 in sera and tumours from patients with colonic neoplasia with and without acromegalyF Miraki-Moud
Department of Endocrinology, St. Bartholomew's Hospital, London, UK
Clin Endocrinol (Oxf) 54:499-508. 2001.... - Cloning and characterization of ERF-1, a human member of the Tis11 family of early-response genesS A Bustin
Surgical Unit, London Hospital Medical College, University of London, UK
DNA Cell Biol 13:449-59. 1994..The mRNA contains a consensus polyadenylation signal... 
Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patientsS A Bustin
Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, UK
Br J Cancer 79:1813-20. 1999..We conclude that neither ck19 nor ck20 are reliable markers for the detection of colon epithelial cells in peripheral blood and that an evaluation of the usefulness of GCC awaits further longitudinal studies...- Tumour-infiltrating lymphocytes in colorectal cancer with microsatellite instability are activated and cytotoxicS M Phillips
Academic Department of Surgery, Royal London Hospital, London, UK
Br J Surg 91:469-75. 2004..020) and a significantly higher level of expression of IL-2Ralpha (P = 0.017). CONCLUSION: The results support the hypothesis that MSI-H colorectal cancers may be more immunogenic than MSS tumours... - Molecular quantification and mapping of lymph-node micrometastases in cervical cancerP O Van Trappen
Academic Department of Gynaecological Oncology, Queen Mary and Westfield College, St Bartholomew s Hospital, London, UK
Lancet 357:15-20. 2001..Reverse-transcriptase PCR (RT-PCR) is a highly sensitive method for detection of lymph-node micrometastases, but accurate quantitative assessment has been difficult... - Identification of differentially expressed genes associated with colorectal cancer liver metastasisS R Li
Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
Eur Surg Res 35:327-36. 2003.... - An enzyme-linked immunosorbent assay for the detection of agents which interfere with the DNA binding activities of transcription factors--exemplified by NF-IL6I A McKay
Centre for Cutaneous Research, London, England
Anal Biochem 265:28-34. 1998..Using the NF-IL6 transcription factor we show that the assay can detect reductions to 86% at significant levels. The assay may be readily adapted for robotic manipulation, making it ideal for high-throughput screening... - Altered monocyte cyclo-oxygenase response in non-obese diabetic miceH Beyan
Centre for Diabetes and Metabolic Medicine DMM, Institute of Cell and Molecular Science, London, UK
Clin Exp Immunol 155:304-10. 2009..However, Vioxx failed to affect insulitis or diabetes. We conclude that monocyte responses are altered in diabetic NOD mice but COX-2 expression is unlikely to be critical to disease risk... - Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase mRNA in individuals with colorectal cancerB W Ogunkolade
Academic Department of Surgery, Barts and The London, Queen Mary's School of Medicine and Dentistry, University of London, London E1 1BB, UK
Lancet 359:1831-2. 2002.... - The expression of the growth hormone secretagogue receptor ligand ghrelin in normal and abnormal human pituitary and other neuroendocrine tumorsM Korbonits
Department of Endocrinology, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, United Kingdom EC1A 7BE
J Clin Endocrinol Metab 86:881-7. 2001..We suggest that the presence of ghrelin mRNA and peptide in the pituitary implies that the locally synthesized hormone may have an autocrine/paracrine modulatory effect on pituitary hormone release... - ERF-2, the human homologue of the murine Tis11d early response geneX F Nie
Surgical Unit, London Hospital Medical College, University of London, UK
Gene 152:285-6. 1995..The sequence has transactivation-like motifs, an unusual Cys-Ser-Ala-rich motif and displays sequence similarity at the extreme C-terminal end with another Tis11 family member, ERF-1... - Quantitative real-time RT-PCR--a perspectiveS A Bustin
Institute of Cellular and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK
J Mol Endocrinol 34:597-601. 2005.... - Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problemsS A Bustin
Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, UK
J Mol Endocrinol 29:23-39. 2002.... - Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase C mRNA levels in lymph nodes may not predict treatment failure in colorectal cancer patientsStephen A Bustin
Centre for Academic Surgery, Barts and The London Queen Mary s School of Medicine and Dentistry, London, United Kingdom
Int J Cancer 108:412-7. 2004..Instead, accurate prognostic stratification requires careful assessment of the likely metastatic potential of the primary cancer... - Quantification of mRNA using real-time RT-PCRTania Nolan
Sigma-Aldrich, Homefield Road, Haverhill, UK
Nat Protoc 1:1559-82. 2006..The entire protocol described here, encompassing all stages from initial assay design to reliable qPCR data analysis, requires approximately 15 h... - Altered monocyte cyclooxygenase response to lipopolysaccharide in type 1 diabetesHuriya Beyan
Department of Diabetes and Metabolic Medicine, Institute of Cell and Molecular Science, London E1 2AT, U K
Diabetes 55:3439-45. 2006..Because COX-2 response to LPS is proinflammatory, an inherited reduced response would predispose to chronic inflammatory diseases such as type 1 diabetes... - Vitamin D metabolism in peripheral blood mononuclear cells is influenced by chewing "betel nut" (Areca catechu) and vitamin D statusWilliam B Ogunkolade
Center for Diabetes and Metabolic Medicine, Royal London Hospital, London E1 1BB, United Kingdom
J Clin Endocrinol Metab 91:2612-7. 2006.... - SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparationsTania Nolan
Sigma-Aldrich, Haverhill CB9 8QP, UK
Anal Biochem 351:308-10. 2006 - Real-time reverse transcription PCR and the detection of occult disease in colorectal cancerStephen A Bustin
Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, UK
Mol Aspects Med 27:192-223. 2006.... - Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosisStephen A Bustin
Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, U K
Clin Sci (Lond) 109:365-79. 2005.... - The GH-IGF-I axis and breast cancerChristiana Laban
Department of Breast Surgery, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE, UK
Trends Endocrinol Metab 14:28-34. 2003..Here, we discuss the influence of the GH-IGF-I axis in normal mammary development and homeostasis, its putative role in breast tumorigenesis and its interactions with estrogen signalling... - Gene expression profiling for molecular staging and prognosis prediction in colorectal cancerStephen A Bustin
Royal London Hospital, London E1 1BB, UK
Expert Rev Mol Diagn 4:599-607. 2004..In turn, this may allow a clinically relevant definition of patient subgroups based on individual molecular parameters for rational decision making regarding choice of therapy... - Expression of PRPF31 mRNA in patients with autosomal dominant retinitis pigmentosa: a molecular clue for incomplete penetrance?Eranga N Vithana
Division of Molecular Genetics, Institute of Ophthalmology, University College London, London, UK
Invest Ophthalmol Vis Sci 44:4204-9. 2003..4 (RP11) with mutations in the PRPF31 gene is due to differentially expressed wild-type alleles in symptomatic and asymptomatic individuals... - Pitfalls of quantitative real-time reverse-transcription polymerase chain reactionStephen A Bustin
Centre for Academic Surgery Institute of Cell and Molecular Science Barts, University of London, UK
J Biomol Tech 15:155-66. 2004.... 
Colorectal cancers with microsatellite instability display mRNA expression signatures characteristic of increased immunogenicityAyan Banerjea
Centre for Academic Surgery, Barts and the London Queen Mary School of Medicine and Dentistry, The Royal London Hospital, Whitechapel, London, E1 1BB, UK
Mol Cancer 3:21. 2004..We aimed to compare the gene expression profiles of MSI-H and MSS cancers to provide evidence for an activated immune response in the former...- Differential expression patterns of the insulin-like growth factor 2 gene in human colorectal cancerShu Rui Li
Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, London, UK
Tumour Biol 25:62-8. 2004..Also, individuals with loss of genomic imprinting (LOI) causing over-expression of IGF 2 may be at greater risk of developing CRC. However, this LOI may be reversed in MSI-H patients... - Evidence for a link between IGF-I and cancerPaul J Jenkins
Department of Endocrinology, St Bartholomew s Hospital, London EC1A 7BE, UK
Eur J Endocrinol 151:S17-22. 2004..The increased understanding of the molecular mechanisms and signalling pathways regulated by the GH/IGF-I axis has started to provide significant insights into the aetiology, prevention and therapy for a number of common cancers...