S A Bustin

Summary

Affiliation: Queen Mary
Country: UK

Publications

  1. ncbi request reprint Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences
    Stephen A Bustin
    The Royal London Hospital, Centre for Academic Surgery, 4th Floor Alexandra Wing, London, E1 1BB, UK
    Expert Rev Mol Diagn 5:493-8. 2005
  2. ncbi request reprint Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    Carmela Tricarico
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy
    Anal Biochem 309:293-300. 2002
  3. ncbi request reprint Cytokeratin 20 is not a tissue-specific marker for the detection of malignant epithelial cells in the blood of colorectal cancer patients
    S A Bustin
    Academic Department of Surgery, St Bartholomew s, Royal London School of Medicine and Dentistry, London, England
    Int J Surg Investig 2:49-57. 2000
  4. ncbi request reprint The growth hormone-insulin-like growth factor-I axis and colorectal cancer
    S A Bustin
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK E1 1BB
    Trends Mol Med 7:447-54. 2001
  5. ncbi request reprint Expression of HLA class II in colorectal cancer: evidence for enhanced immunogenicity of microsatellite-instability-positive tumours
    S A Bustin
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, London, UK
    Tumour Biol 22:294-8. 2001
  6. ncbi request reprint Expression of the Ca2+-activated chloride channel genes CLCA1 and CLCA2 is downregulated in human colorectal cancer
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
    DNA Cell Biol 20:331-8. 2001
  7. ncbi request reprint Local expression of insulin-like growth factor-I affects angiogenesis in colorectal cancer
    Stephen A Bustin
    Academic Department of Surgery, Barts and The London Queen Mary s School of Medicine and Dentistry, London, UK
    Tumour Biol 23:130-8. 2002
  8. ncbi request reprint Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London E1 1BB, UK
    J Mol Endocrinol 25:169-93. 2000
  9. ncbi request reprint The product of the primary response gene BRF1 inhibits the interaction between 14-3-3 proteins and cRaf-1 in the yeast trihybrid system
    S A Bustin
    Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, England
    DNA Cell Biol 18:653-61. 1999
  10. ncbi request reprint Molecular assessment of tumour stage and disease recurrence using PCR-based assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s, London, UK
    Mol Med Today 4:389-96. 1998

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences
    Stephen A Bustin
    The Royal London Hospital, Centre for Academic Surgery, 4th Floor Alexandra Wing, London, E1 1BB, UK
    Expert Rev Mol Diagn 5:493-8. 2005
    ..These results emphasize the urgent need for the establishment of best practice guidelines for this technology, particularly in the context of its mounting adaptation as a high-throughput clinical diagnostic assay...
  2. ncbi request reprint Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    Carmela Tricarico
    Clinical Biochemistry Unit, Department of Clinical Physiopathology, University of Florence, Florence, Italy
    Anal Biochem 309:293-300. 2002
    ..Our results suggest that the use of internal standards comprising single housekeeping genes or rRNA is inappropriate for studies involving tissue biopsies...
  3. ncbi request reprint Cytokeratin 20 is not a tissue-specific marker for the detection of malignant epithelial cells in the blood of colorectal cancer patients
    S A Bustin
    Academic Department of Surgery, St Bartholomew s, Royal London School of Medicine and Dentistry, London, England
    Int J Surg Investig 2:49-57. 2000
    ..Cytokeratin 20 has been proposed as a tissue-specific marker for the detection of micrometastases in the blood of colorectal cancer patients. However, recent reports have challenged its specificity, and hence the validity of its use...
  4. ncbi request reprint The growth hormone-insulin-like growth factor-I axis and colorectal cancer
    S A Bustin
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK E1 1BB
    Trends Mol Med 7:447-54. 2001
    ..However, the molecular mechanisms and signalling pathways responsible are only beginning to be unravelled, as are the relative contributions of the endocrine and autocrine or paracrine effects...
  5. ncbi request reprint Expression of HLA class II in colorectal cancer: evidence for enhanced immunogenicity of microsatellite-instability-positive tumours
    S A Bustin
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, London, UK
    Tumour Biol 22:294-8. 2001
    ....
  6. ncbi request reprint Expression of the Ca2+-activated chloride channel genes CLCA1 and CLCA2 is downregulated in human colorectal cancer
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
    DNA Cell Biol 20:331-8. 2001
    ..Our results suggest that CLCA1 could specify a new tumor suppressor and that, as in breast cancer, CLCA2 may function as a tumor suppressor in colorectal cancer...
  7. ncbi request reprint Local expression of insulin-like growth factor-I affects angiogenesis in colorectal cancer
    Stephen A Bustin
    Academic Department of Surgery, Barts and The London Queen Mary s School of Medicine and Dentistry, London, UK
    Tumour Biol 23:130-8. 2002
    ..The biological relevance of this finding is suggested by the significant association between local IGF-I mRNA levels and microvessel density in the colorectal cancers...
  8. ncbi request reprint Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London E1 1BB, UK
    J Mol Endocrinol 25:169-93. 2000
    ..It illustrates the usefulness of these assays by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH)...
  9. ncbi request reprint The product of the primary response gene BRF1 inhibits the interaction between 14-3-3 proteins and cRaf-1 in the yeast trihybrid system
    S A Bustin
    Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, England
    DNA Cell Biol 18:653-61. 1999
    ....
  10. ncbi request reprint Molecular assessment of tumour stage and disease recurrence using PCR-based assays
    S A Bustin
    Academic Department of Surgery, St Bartholomew s, London, UK
    Mol Med Today 4:389-96. 1998
    ..Its role in the identification of minimal residual disease after curative surgical resection requires clinical validation in further prospective studies...
  11. ncbi request reprint Increased levels of insulin-like growth factor binding protein-2 in sera and tumours from patients with colonic neoplasia with and without acromegaly
    F Miraki-Moud
    Department of Endocrinology, St. Bartholomew's Hospital, London, UK
    Clin Endocrinol (Oxf) 54:499-508. 2001
    ....
  12. ncbi request reprint Cloning and characterization of ERF-1, a human member of the Tis11 family of early-response genes
    S A Bustin
    Surgical Unit, London Hospital Medical College, University of London, UK
    DNA Cell Biol 13:449-59. 1994
    ..The mRNA contains a consensus polyadenylation signal...
  13. pmc Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients
    S A Bustin
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, UK
    Br J Cancer 79:1813-20. 1999
    ..We conclude that neither ck19 nor ck20 are reliable markers for the detection of colon epithelial cells in peripheral blood and that an evaluation of the usefulness of GCC awaits further longitudinal studies...
  14. ncbi request reprint Tumour-infiltrating lymphocytes in colorectal cancer with microsatellite instability are activated and cytotoxic
    S M Phillips
    Academic Department of Surgery, Royal London Hospital, London, UK
    Br J Surg 91:469-75. 2004
    ..The nature of tumour-infiltrating lymphocytes in colorectal cancers was investigated using quantitative real-time polymerase chain reaction (PCR) and immunohistochemistry...
  15. ncbi request reprint Molecular quantification and mapping of lymph-node micrometastases in cervical cancer
    P O Van Trappen
    Academic Department of Gynaecological Oncology, Queen Mary and Westfield College, St Bartholomew s Hospital, London, UK
    Lancet 357:15-20. 2001
    ..Reverse-transcriptase PCR (RT-PCR) is a highly sensitive method for detection of lymph-node micrometastases, but accurate quantitative assessment has been difficult...
  16. ncbi request reprint Identification of differentially expressed genes associated with colorectal cancer liver metastasis
    S R Li
    Academic Department of Surgery, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, UK
    Eur Surg Res 35:327-36. 2003
    ....
  17. ncbi request reprint An enzyme-linked immunosorbent assay for the detection of agents which interfere with the DNA binding activities of transcription factors--exemplified by NF-IL6
    I A McKay
    Centre for Cutaneous Research, London, England
    Anal Biochem 265:28-34. 1998
    ..Using the NF-IL6 transcription factor we show that the assay can detect reductions to 86% at significant levels. The assay may be readily adapted for robotic manipulation, making it ideal for high-throughput screening...
  18. pmc Altered monocyte cyclo-oxygenase response in non-obese diabetic mice
    H Beyan
    Centre for Diabetes and Metabolic Medicine DMM, Institute of Cell and Molecular Science, London, UK
    Clin Exp Immunol 155:304-10. 2009
    ..However, Vioxx failed to affect insulitis or diabetes. We conclude that monocyte responses are altered in diabetic NOD mice but COX-2 expression is unlikely to be critical to disease risk...
  19. ncbi request reprint Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase mRNA in individuals with colorectal cancer
    B W Ogunkolade
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, UK
    Lancet 359:1831-2. 2002
    ....
  20. ncbi request reprint The expression of the growth hormone secretagogue receptor ligand ghrelin in normal and abnormal human pituitary and other neuroendocrine tumors
    M Korbonits
    Department of Endocrinology, St Bartholomew s and the Royal London School of Medicine and Dentistry, London, United Kingdom EC1A 7BE
    J Clin Endocrinol Metab 86:881-7. 2001
    ..We suggest that the presence of ghrelin mRNA and peptide in the pituitary implies that the locally synthesized hormone may have an autocrine/paracrine modulatory effect on pituitary hormone release...
  21. ncbi request reprint ERF-2, the human homologue of the murine Tis11d early response gene
    X F Nie
    Surgical Unit, London Hospital Medical College, University of London, UK
    Gene 152:285-6. 1995
    ..The sequence has transactivation-like motifs, an unusual Cys-Ser-Ala-rich motif and displays sequence similarity at the extreme C-terminal end with another Tis11 family member, ERF-1...
  22. ncbi request reprint Quantitative real-time RT-PCR--a perspective
    S A Bustin
    Institute of Cellular and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London, UK
    J Mol Endocrinol 34:597-601. 2005
    ....
  23. ncbi request reprint Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
    S A Bustin
    Academic Department of Surgery, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, UK
    J Mol Endocrinol 29:23-39. 2002
    ....
  24. ncbi request reprint Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase C mRNA levels in lymph nodes may not predict treatment failure in colorectal cancer patients
    Stephen A Bustin
    Centre for Academic Surgery, Barts and The London Queen Mary s School of Medicine and Dentistry, London, United Kingdom
    Int J Cancer 108:412-7. 2004
    ..Instead, accurate prognostic stratification requires careful assessment of the likely metastatic potential of the primary cancer...
  25. ncbi request reprint Quantification of mRNA using real-time RT-PCR
    Tania Nolan
    Sigma Aldrich, Homefield Road, Haverhill, UK
    Nat Protoc 1:1559-82. 2006
    ..The entire protocol described here, encompassing all stages from initial assay design to reliable qPCR data analysis, requires approximately 15 h...
  26. ncbi request reprint Altered monocyte cyclooxygenase response to lipopolysaccharide in type 1 diabetes
    Huriya Beyan
    Department of Diabetes and Metabolic Medicine, Institute of Cell and Molecular Science, London E1 2AT, U K
    Diabetes 55:3439-45. 2006
    ..Because COX-2 response to LPS is proinflammatory, an inherited reduced response would predispose to chronic inflammatory diseases such as type 1 diabetes...
  27. ncbi request reprint Vitamin D metabolism in peripheral blood mononuclear cells is influenced by chewing "betel nut" (Areca catechu) and vitamin D status
    William B Ogunkolade
    Center for Diabetes and Metabolic Medicine, Royal London Hospital, London E1 1BB, United Kingdom
    J Clin Endocrinol Metab 91:2612-7. 2006
    ..We previously reported that circulating 1,25(OH)(2)D contributed to determination of VDR-protein levels and VDR genotype was a determinant of both VDR mRNA and VDR-protein in South Asians...
  28. ncbi request reprint SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
    Tania Nolan
    Sigma Aldrich, Haverhill CB9 8QP, UK
    Anal Biochem 351:308-10. 2006
  29. ncbi request reprint Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer
    Stephen A Bustin
    Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, UK
    Mol Aspects Med 27:192-223. 2006
    ....
  30. ncbi request reprint Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
    Stephen A Bustin
    Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, University of London, London E1 1BB, U K
    Clin Sci (Lond) 109:365-79. 2005
    ....
  31. ncbi request reprint The GH-IGF-I axis and breast cancer
    Christiana Laban
    Department of Breast Surgery, St Bartholomew s Hospital, West Smithfield, London EC1A 7BE, UK
    Trends Endocrinol Metab 14:28-34. 2003
    ..Here, we discuss the influence of the GH-IGF-I axis in normal mammary development and homeostasis, its putative role in breast tumorigenesis and its interactions with estrogen signalling...
  32. ncbi request reprint Gene expression profiling for molecular staging and prognosis prediction in colorectal cancer
    Stephen A Bustin
    Royal London Hospital, London E1 1BB, UK
    Expert Rev Mol Diagn 4:599-607. 2004
    ..In turn, this may allow a clinically relevant definition of patient subgroups based on individual molecular parameters for rational decision making regarding choice of therapy...
  33. ncbi request reprint Expression of PRPF31 mRNA in patients with autosomal dominant retinitis pigmentosa: a molecular clue for incomplete penetrance?
    Eranga N Vithana
    Division of Molecular Genetics, Institute of Ophthalmology, University College London, London, UK
    Invest Ophthalmol Vis Sci 44:4204-9. 2003
    ..4 (RP11) with mutations in the PRPF31 gene is due to differentially expressed wild-type alleles in symptomatic and asymptomatic individuals...
  34. pmc Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
    Stephen A Bustin
    Centre for Academic Surgery Institute of Cell and Molecular Science Barts, University of London, UK
    J Biomol Tech 15:155-66. 2004
    ....
  35. pmc Colorectal cancers with microsatellite instability display mRNA expression signatures characteristic of increased immunogenicity
    Ayan Banerjea
    Centre for Academic Surgery, Barts and the London Queen Mary School of Medicine and Dentistry, The Royal London Hospital, Whitechapel, London, E1 1BB, UK
    Mol Cancer 3:21. 2004
    ..We aimed to compare the gene expression profiles of MSI-H and MSS cancers to provide evidence for an activated immune response in the former...
  36. ncbi request reprint Differential expression patterns of the insulin-like growth factor 2 gene in human colorectal cancer
    Shu Rui Li
    Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and The London, Queen Mary s School of Medicine and Dentistry, London, UK
    Tumour Biol 25:62-8. 2004
    ..Also, individuals with loss of genomic imprinting (LOI) causing over-expression of IGF 2 may be at greater risk of developing CRC. However, this LOI may be reversed in MSI-H patients...
  37. ncbi request reprint Evidence for a link between IGF-I and cancer
    Paul J Jenkins
    Department of Endocrinology, St Bartholomew s Hospital, London EC1A 7BE, UK
    Eur J Endocrinol 151:S17-22. 2004
    ..The increased understanding of the molecular mechanisms and signalling pathways regulated by the GH/IGF-I axis has started to provide significant insights into the aetiology, prevention and therapy for a number of common cancers...