E Diane Williamson

Summary

Affiliation: Porton Down
Country: UK

Publications

  1. pmc A recombinant carboxy-terminal domain of the protective antigen of Bacillus anthracis protects mice against anthrax infection
    Helen C Flick-Smith
    Dstl, Chemical and Biological Sciences, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom
    Infect Immun 70:1653-6. 2002
  2. doi request reprint The natural history and incidence of Yersinia pestis and prospects for vaccination
    E D Williamson
    Biomedical Sciences, Dstl Porton Down, Salisbury SP4 0JQ, UK
    J Med Microbiol 61:911-8. 2012
  3. pmc Protecting against plague: towards a next-generation vaccine
    E D Williamson
    Biomedical Sciences Department, Defence Science and Technology Laboratory, Salisbury, Wilts, UK
    Clin Exp Immunol 172:1-8. 2013
  4. pmc Immunogenicity of recombinant protective antigen and efficacy against aerosol challenge with anthrax
    E D Williamson
    Defence Science and Technology Laboratory Porton Down, Salisbury, Wilts SP4 0JQ, United Kingdom
    Infect Immun 73:5978-87. 2005
  5. ncbi request reprint Co-immunisation with a plasmid DNA cocktail primes mice against anthrax and plague
    E D Williamson
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 OJQ, UK
    Vaccine 20:2933-41. 2002
  6. ncbi request reprint Kinetics of the immune response to the (F1+V) vaccine in models of bubonic and pneumonic plague
    E D Williamson
    Dstl Porton Down, Salisbury, Wilts SP4 0JQ, UK
    Vaccine 25:1142-8. 2007
  7. ncbi request reprint Immunogenicity of the rF1+rV vaccine for plague with identification of potential immune correlates
    E D Williamson
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wilts UK SP4 0JQ, UK
    Microb Pathog 42:11-21. 2007
  8. doi request reprint Plague
    E D Williamson
    Defence Science and Technology Laboratory Dstl, Porton Down, Salisbury, Wilts SP4 0JQ, UK
    Vaccine 27:D56-60. 2009
  9. doi request reprint Predictive models and correlates of protection for testing biodefence vaccines
    E Diane Williamson
    Defence Science and Technology Laboratory, Porton Down, Salisbury, SP4 0JQ, UK
    Expert Rev Vaccines 9:527-37. 2010
  10. doi request reprint Recombinant (F1+V) vaccine protects cynomolgus macaques against pneumonic plague
    E D Williamson
    Dstl PortonDown, Salisbury, Wilts SP4 0JQ, UK
    Vaccine 29:4771-7. 2011

Collaborators

Detail Information

Publications45

  1. pmc A recombinant carboxy-terminal domain of the protective antigen of Bacillus anthracis protects mice against anthrax infection
    Helen C Flick-Smith
    Dstl, Chemical and Biological Sciences, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom
    Infect Immun 70:1653-6. 2002
    ..Results show that protection can be attributed to individual domains and imply that it is domain 4 which contains the dominant protective epitopes of PA...
  2. doi request reprint The natural history and incidence of Yersinia pestis and prospects for vaccination
    E D Williamson
    Biomedical Sciences, Dstl Porton Down, Salisbury SP4 0JQ, UK
    J Med Microbiol 61:911-8. 2012
    ..The considerable challenges in achieving a vaccine which is licensed for human use and which will comprehensively protect against this serious human pathogen are assessed...
  3. pmc Protecting against plague: towards a next-generation vaccine
    E D Williamson
    Biomedical Sciences Department, Defence Science and Technology Laboratory, Salisbury, Wilts, UK
    Clin Exp Immunol 172:1-8. 2013
    ..pestis, in order to develop a greater understanding of the protective immune responses required to protect against plague...
  4. pmc Immunogenicity of recombinant protective antigen and efficacy against aerosol challenge with anthrax
    E D Williamson
    Defence Science and Technology Laboratory Porton Down, Salisbury, Wilts SP4 0JQ, United Kingdom
    Infect Immun 73:5978-87. 2005
    ..anthracis. These data provide some preliminary evidence for the existence of immune correlates of protection against anthrax infection in rhesus macaques immunized with rPA...
  5. ncbi request reprint Co-immunisation with a plasmid DNA cocktail primes mice against anthrax and plague
    E D Williamson
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 OJQ, UK
    Vaccine 20:2933-41. 2002
    ..pestis compared with priming only with plasmid DNA encoding the V antigen and boosting with rV. This enhancement may be due to the effect of CpG motifs known to be present in the plasmid DNA construct encoding PA...
  6. ncbi request reprint Kinetics of the immune response to the (F1+V) vaccine in models of bubonic and pneumonic plague
    E D Williamson
    Dstl Porton Down, Salisbury, Wilts SP4 0JQ, UK
    Vaccine 25:1142-8. 2007
    ....
  7. ncbi request reprint Immunogenicity of the rF1+rV vaccine for plague with identification of potential immune correlates
    E D Williamson
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wilts UK SP4 0JQ, UK
    Microb Pathog 42:11-21. 2007
    ..Serum samples from representative macaques within this time period also inhibited the Yersinia-mediated cytotoxicity of J774 macrophages in a qualitative in vitro assay of type three secretion...
  8. doi request reprint Plague
    E D Williamson
    Defence Science and Technology Laboratory Dstl, Porton Down, Salisbury, Wilts SP4 0JQ, UK
    Vaccine 27:D56-60. 2009
    ..This paper reviews the progress towards an improved vaccine for plague and assesses the likely impact of a prophylactic vaccine for bubonic and pneumonic plague...
  9. doi request reprint Predictive models and correlates of protection for testing biodefence vaccines
    E Diane Williamson
    Defence Science and Technology Laboratory, Porton Down, Salisbury, SP4 0JQ, UK
    Expert Rev Vaccines 9:527-37. 2010
    ..This review summarizes some of the immune correlates data reported for biodefence vaccines as well as some of the analytical approaches that can be applied in order to predict clinical efficacy...
  10. doi request reprint Recombinant (F1+V) vaccine protects cynomolgus macaques against pneumonic plague
    E D Williamson
    Dstl PortonDown, Salisbury, Wilts SP4 0JQ, UK
    Vaccine 29:4771-7. 2011
    ..This candidate vaccine, which has been evaluated as safe and immunogenic in clinical studies, has now been demonstrated to protect cynomolgus macaques, immunised in the clinical regimen, against pneumonic plague...
  11. doi request reprint N255 is a key residue for recognition by a monoclonal antibody which protects against Yersinia pestis infection
    Jim Hill
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    Vaccine 27:7073-9. 2009
    ..3 protects by binding to LcrV(Ype) and interfering with protein-protein interactions necessary for type three secretion...
  12. ncbi request reprint Stat 4 but not Stat 6 mediated immune mechanisms are essential in protection against plague
    Stephen J Elvin
    Defence Science and Technology Laboratories, Porton Down, Salisbury SP4 0JQ, UK
    Microb Pathog 37:177-84. 2004
    ..It appears therefore that type 1 immune mechanisms, activated following Stat 4 phosphorylation, are essential in protection against plague...
  13. ncbi request reprint Protection against bubonic and pneumonic plague with a single dose microencapsulated sub-unit vaccine
    Stephen J Elvin
    Dstl, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    Vaccine 24:4433-9. 2006
    ..Microencapsulation of these vaccine antigens has the added advantage that controlled release of the antigens occurs in vivo, so that protective immunity can be induced after only a single immunising dose...
  14. pmc Protection against heterologous Burkholderia pseudomallei strains by dendritic cell immunization
    Stephen J Elvin
    Biomedical Sciences, Dstl Porton Down, Salisbury SP4 0JQ, United Kingdom
    Infect Immun 74:1706-11. 2006
    ..These results show that a vaccine strategy that actively targets dendritic cells can evoke protective immune responses...
  15. ncbi request reprint Immunological responses after immunisation of mice with microparticles containing antigen and single stranded RNA (polyuridylic acid)
    Angie Westwood
    Building 7A, Dstl, Porton Down, Salisbury SP4 0JQ, UK
    Vaccine 24:1736-43. 2006
    ..05). These data demonstrate, for the first time, that appropriately formulated ssRNA can act as a potent adjuvant and modulator of adaptive immunological responses...
  16. ncbi request reprint Mucosal delivery of microparticle encapsulated ESAT-6 induces robust cell-mediated responses in the lung milieu
    Zoë K Carpenter
    Biomedical Sciences, Dstl, Porton Down, Salisbury, SP4 0JQ, UK
    J Control Release 104:67-77. 2005
    ..Furthermore, our data indicate that, for efficient activation of cell-mediated responses, antigens must be presented to the immune system in an appropriate formulation...
  17. pmc Mucosal or parenteral administration of microsphere-associated Bacillus anthracis protective antigen protects against anthrax infection in mice
    Helen C Flick-Smith
    Dstl, Chemical and Biological Sciences, Porton Down, Salisbury, Wiltshire, SP4 0JQ, United Kingdom
    Infect Immun 70:2022-8. 2002
    ....
  18. pmc Synergistic protection of mice against plague with monoclonal antibodies specific for the F1 and V antigens of Yersinia pestis
    Jim Hill
    Defence Science and Technology Laboratory, Porton Down, Wiltshire SP4 OJQ, United Kingdom
    Infect Immun 71:2234-8. 2003
    ..Antibodies showed synergy when administered prophylactically and as a therapy 48 h postinfection. Monoclonal antibodies therefore have potential as a treatment for plague...
  19. ncbi request reprint Yersinia pestis (plague) vaccines
    Richard W Titball
    Dstl Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK
    Expert Opin Biol Ther 4:965-73. 2004
    ..Of these, an injected subunit vaccine is likely to offer the best near-term solution to the provision of a vaccine that protects against both bubonic and pneumonic plague...
  20. pmc Humoral and cell-mediated adaptive immune responses are required for protection against Burkholderia pseudomallei challenge and bacterial clearance postinfection
    Gareth D Healey
    Host Pathogen Analysis, Bldg 7A, Rm 201, Dstl, Porton Down, Salisbury SP4 0JQ, United Kingdom
    Infect Immun 73:5945-51. 2005
    ..These results indicate the importance of both cell-mediated and humoral immune mechanisms in protection against intracellular pathogens...
  21. pmc Antibiotic-free plasmid stabilization by operator-repressor titration for vaccine delivery by using live Salmonella enterica Serovar typhimurium
    Helen S Garmory
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, United Kingdom
    Infect Immun 73:2005-11. 2005
    ..This technology can easily be used to convert any suitable attenuated strain to an antibiotic-free ORT strain for recombinant protein vaccine delivery in humans...
  22. ncbi request reprint Second and third generation plague vaccines
    Richard W Titball
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    Adv Exp Med Biol 529:397-406. 2003
  23. ncbi request reprint The use of live attenuated bacteria as a delivery system for heterologous antigens
    Helen S Garmory
    Department of Biomedical Sciences, Salisbury, UK
    J Drug Target 11:471-9. 2003
    ..In this review, these strategies and their use in the development of a delivery system for the Yersinia pestis V antigen are described...
  24. ncbi request reprint Evolutionary genetics: Ambiguous role of CCR5 in Y. pestis infection
    Stephen J Elvin
    Defence Science and Technology Laboratories, Porton Down, Salisbury SP4 0JQ, UK
    Nature 430:417. 2004
    ..pestis by Ccr5-deficient macrophages in vitro. Our results indicate that the role of Ccr5 in Y. pestis infection may therefore be more complex than previously thought...
  25. ncbi request reprint Activation of dendritic cells by microparticles containing Bacillus anthracis protective antigen
    Angie Westwood
    Biomedical Sciences, Dstl, Porton Down, Salisbury SP4 0JQ, UK
    Vaccine 23:3857-63. 2005
    ....
  26. pmc Protection against experimental melioidosis following immunization with live Burkholderia thailandensis expressing a manno-heptose capsule
    Andrew E Scott
    Defence Science and Technology Laboratory, Porton Down, Salisbury, United Kingdom
    Clin Vaccine Immunol 20:1041-7. 2013
    ..E555-immunized mice had significantly higher levels of IgG than mice immunized with noncapsulated B. thailandensis, and these antibody responses were primarily directed against the capsule. ..
  27. ncbi request reprint Distribution of productive antigen-processing activity for MHC class II presentation in macrophages
    A von Delwig
    Musculoskeletal Research Group, Clinical Medical Sciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, UK
    Scand J Immunol 62:243-50. 2005
    ..The data suggest that endosomal compartments expressing Rab5 guanosine triphosphatase can productively process protein antigens for presentation by mature MHC class II molecules...
  28. ncbi request reprint A biocompatible microdevice for core body temperature monitoring in the early diagnosis of infectious disease
    E D Williamson
    Dstl Porton Down, Salisbury, Wilts, UK, SP4 0JQ
    Biomed Microdevices 9:51-60. 2007
    ....
  29. pmc The fraction 1 and V protein antigens of Yersinia pestis activate dendritic cells to induce primary T cell responses
    R Kingston
    Antigen Presentation Research Group, Imperial College London, Northwick Park and St Mark s Campus, Watford Road, Harrow, UK
    Clin Exp Immunol 149:561-9. 2007
    ..This study suggests an important role for DC, or factors secreted by them, in the induction of protective immunity to plague by the rF1 and rV antigens...
  30. pmc Human immune response to a plague vaccine comprising recombinant F1 and V antigens
    E D Williamson
    Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom
    Infect Immun 73:3598-608. 2005
    ..Potential serological immune correlates of protection have been investigated, but no trends specific to vaccination could be detected in cellular markers...
  31. ncbi request reprint An aroA mutant of Yersinia pestis is attenuated in guinea-pigs, but virulent in mice
    P C Oyston
    Chemical and Biological Defence Establishment, Salisbury, Wiltshire, UK
    Microbiology 142:1847-53. 1996
    ..Unusually for an aro-defective mutant, the Y. pestis aroA mutant was virulent in mice, with a median dose which induced morbidity of death similar to that of the wild-type, although time to death was significantly prolonged...
  32. ncbi request reprint Tissue distribution of radioactivity following intranasal administration of radioactive microspheres
    J E Eyles
    Pharmaceutical Sciences, Life and Health Sciences, Aston University, Birmingham, UK
    J Pharm Pharmacol 53:601-7. 2001
    ..This effect may contribute to the effectiveness of pulmonary delivered antigen-loaded microparticles as humoral immunogens...
  33. ncbi request reprint Passive transfer of protection against Bacillus anthracis infection in a murine model
    R J Beedham
    Pathobiology, CBD, DERA Porton Down, Salisbury, SP4 0JQ, Wiltshire, UK
    Vaccine 19:4409-16. 2001
    ..The results demonstrated that an antibody response maybe important in protection against B. anthracis infection, under the conditions tested. The results provide further data for the development of an improved anthrax vaccine...
  34. ncbi request reprint Microsphere translocation and immunopotentiation in systemic tissues following intranasal administration
    J E Eyles
    DERA (Chemical and Biological Defence Sector, Porton Down, Wiltshire SP4 OJQ, Salisbury, UK
    Vaccine 19:4732-42. 2001
    ....
  35. ncbi request reprint Molecular variation between the alpha-toxins from the type strain (NCTC 8237) and clinical isolates of Clostridium perfringens associated with disease in man and animals
    A Ginter
    Division Immunologie Animale, Centre d Economie Rurale, Marloie, Belgium
    Microbiology 142:191-8. 1996
    ..The changes in amino acid sequence did not affect the ability of a C-terminal domain vaccine, derived from the alpha-toxin of strain NCTC 8237, to induce protection against the alpha-toxin from a bovine enteric strain of C. perfringens...
  36. ncbi request reprint Protection against plague following immunisation with microencapsulated V antigen is reduced by co-encapsulation with IFN-gamma or IL-4, but not IL-6
    K F Griffin
    Dstl Biomedical Sciences, Porton Down, Salisbury, SP4 0JQ, Wiltshire, UK
    Vaccine 20:3650-7. 2002
    ..pestis strain GB; however protective efficacy was impaired by co-encapsulating either IFN-gamma or IL-4 with rV...
  37. ncbi request reprint Stimulation of spleen cells in vitro by nanospheric particles containing antigen
    J E Eyles
    Dstl, Porton Down, SP4 0JQ, Salisbury, UK
    J Control Release 86:25-32. 2003
    ..This mechanism is likely to be distinct from non-specific effects caused by components of the delivery vehicle itself...
  38. ncbi request reprint Protective efficacy of a fully recombinant plague vaccine in the guinea pig
    S M Jones
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    Vaccine 21:3912-8. 2003
    ..Cross-protection of the mouse with guinea pig IgG suggests that the recognition of neutralising epitopes in the F1 and V proteins is conserved between these two species...
  39. ncbi request reprint Vaccine development for potential bioterrorism agents
    R W Titball
    Defence Science and Technology Laboratory, Porton Down, Salisbury, UK
    Curr Drug Targets Infect Disord 3:255-62. 2003
    ..The prospects for the development of a new generation of bioterrorism vaccines which exploit these technologies are reviewed in this manuscript...
  40. ncbi request reprint Immunological aspects of polymer microsphere vaccine delivery systems
    J E Eyles
    Biomedical Sciences, Dstl, Porton Downs, Salisbury, UK
    J Drug Target 11:509-14. 2003
    ..These data support the tenet that microencapsulation serves to modify the uptake, trafficking and processing of antigens...
  41. ncbi request reprint Immunisation against plague by transcutaneous and intradermal application of subunit antigens
    J E Eyles
    Biomedical Sciences, Dstl, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    Vaccine 22:4365-73. 2004
    ..These data suggest that transcutaneous immunisation may be a simple and non-invasive method for immunising individuals against plague...
  42. ncbi request reprint Mouse model characterisation for anthrax vaccine development: comparison of one inbred and one outbred mouse strain
    H C Flick-Smith
    Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK
    Microb Pathog 38:33-40. 2005
    ..An assessment of protection in the TO mouse against aerosol challenge with the fully virulent strain of B. anthracis, Ames, was also made...
  43. pmc Probing molecular interactions in intact antibody: antigen complexes, an electrospray time-of-flight mass spectrometry approach
    M A Tito
    Oxford Centre for Molecular Sciences, New Chemistry Laboratory, Oxford OX1 3QT, United Kingdom
    Biophys J 81:3503-9. 2001
    ..More generally this work demonstrates a rapid means of assessing antigen subunit interactions as well as the stoichiometry and specificity of binding in antibody-antigen complexes...
  44. pmc Macromolecular organization of the Yersinia pestis capsular F1 antigen: insights from time-of-flight mass spectrometry
    M A Tito
    Oxford Centre for Molecular Sciences, New Chemistry Laboratory, Oxford, OX1 3QT, United Kingdom
    Protein Sci 10:2408-13. 2001
    ..More generally, the data show that the symmetry and packing of macromolecular complexes can be determined solely from mass spectrometry, without any prior knowledge of higher order structure..
  45. ncbi request reprint Vaccines against dangerous pathogens
    E D Williamson
    Dstl, Chemical and Biological Sciences, Porton Down, Salisbury, UK
    Br Med Bull 62:163-73. 2002
    ..Emphasis is also placed on the derivation of surrogate markers of efficacy and a demonstration that these correlate with protection in the animal model...