P C F Oyston

Summary

Affiliation: Porton Down
Country: UK

Publications

  1. doi request reprint Francisella virulence: significant advances, ongoing challenges and unmet needs
    Petra C F Oyston
    Biomedical Sciences, Dstl Porton Down, Salisbury, UK
    Expert Rev Vaccines 8:1575-85. 2009
  2. pmc A Yersinia pestis guaBA mutant is attenuated in virulence and provides protection against plague in a mouse model of infection
    Petra C F Oyston
    Department of Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom
    Microb Pathog 48:191-5. 2010
  3. doi request reprint The current challenges for vaccine development
    Petra Oyston
    Biomedical Sciences, Dstl Porton Down, Salisbury SP4 0JQ, UK
    J Med Microbiol 61:889-94. 2012
  4. pmc Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux
    Richard A Stabler
    Department of Infectious and Tropical Diseases, Keppel Street, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
    BMC Microbiol 8:177. 2008
  5. pmc Steps toward broad-spectrum therapeutics: discovering virulence-associated genes present in diverse human pathogens
    Chris J Stubben
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA
    BMC Genomics 10:501. 2009
  6. ncbi request reprint Tularemia vaccine: past, present and future
    Petra C F Oyston
    Defence Science and Technology Laboratories, Porton Down, Salisbury, Wiltshire, SP4 0JQ
    Antonie Van Leeuwenhoek 87:277-81. 2005
  7. ncbi request reprint Tularaemia: bioterrorism defence renews interest in Francisella tularensis
    Petra C F Oyston
    Dstl Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK
    Nat Rev Microbiol 2:967-78. 2004
  8. doi request reprint Francisella tularensis: unravelling the secrets of an intracellular pathogen
    Petra C F Oyston
    Biomedical Sciences, Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Med Microbiol 57:921-30. 2008
  9. ncbi request reprint Expression of heterologous O-antigen in Yersinia pestis KIM does not affect virulence by the intravenous route
    P C F Oyston
    Microbiology, Dstl, CBS Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Med Microbiol 52:289-94. 2003
  10. ncbi request reprint Yersinia: an update
    Petra C F Oyston
    Dstl Porton Down, Salisbury, Wiltshire, UK SP4 0JQ
    Trends Microbiol 10:550-1. 2002

Collaborators

Detail Information

Publications48

  1. doi request reprint Francisella virulence: significant advances, ongoing challenges and unmet needs
    Petra C F Oyston
    Biomedical Sciences, Dstl Porton Down, Salisbury, UK
    Expert Rev Vaccines 8:1575-85. 2009
    ..Better animal models to more accurately represent human responses to infection are also required...
  2. pmc A Yersinia pestis guaBA mutant is attenuated in virulence and provides protection against plague in a mouse model of infection
    Petra C F Oyston
    Department of Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom
    Microb Pathog 48:191-5. 2010
    ..pestis parental strain. These findings identify guaBA as a target for deletion to generate a live attenuated plague vaccine...
  3. doi request reprint The current challenges for vaccine development
    Petra Oyston
    Biomedical Sciences, Dstl Porton Down, Salisbury SP4 0JQ, UK
    J Med Microbiol 61:889-94. 2012
    ..In this review, we discuss many of the hurdles that new vaccines must overcome in order to reduce morbidity and mortality, and some of the initiatives that are being attempted to supply new vaccines to those that need them most...
  4. pmc Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux
    Richard A Stabler
    Department of Infectious and Tropical Diseases, Keppel Street, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
    BMC Microbiol 8:177. 2008
    ..We describe the construction and development of an Active Surveillance of Pathogens (ASP) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes...
  5. pmc Steps toward broad-spectrum therapeutics: discovering virulence-associated genes present in diverse human pathogens
    Chris J Stubben
    Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA
    BMC Genomics 10:501. 2009
    ..e., conserved across multiple pathogen species) and novel virulence-associated genes that may serve as targets for broad-spectrum countermeasures...
  6. ncbi request reprint Tularemia vaccine: past, present and future
    Petra C F Oyston
    Defence Science and Technology Laboratories, Porton Down, Salisbury, Wiltshire, SP4 0JQ
    Antonie Van Leeuwenhoek 87:277-81. 2005
    ..Therefore the focus of current research is on producing a new vaccine against tularemia that would be suitable for licensing...
  7. ncbi request reprint Tularaemia: bioterrorism defence renews interest in Francisella tularensis
    Petra C F Oyston
    Dstl Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK
    Nat Rev Microbiol 2:967-78. 2004
    ..This is a reflection of the increased funding of research on this pathogen, particularly in the United States...
  8. doi request reprint Francisella tularensis: unravelling the secrets of an intracellular pathogen
    Petra C F Oyston
    Biomedical Sciences, Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Med Microbiol 57:921-30. 2008
    ..tularensis pathogenicity, and will facilitate the development of a vaccine to protect against infection...
  9. ncbi request reprint Expression of heterologous O-antigen in Yersinia pestis KIM does not affect virulence by the intravenous route
    P C F Oyston
    Microbiology, Dstl, CBS Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Med Microbiol 52:289-94. 2003
    ..Assays showed no alteration in the ability of Y. pestis to resist lysis by cationic antimicrobial peptides, serum or polymyxin...
  10. ncbi request reprint Yersinia: an update
    Petra C F Oyston
    Dstl Porton Down, Salisbury, Wiltshire, UK SP4 0JQ
    Trends Microbiol 10:550-1. 2002
    ..The 8th International Symposium on Yersinia was held in Turku, Finland, 4-8 September 2002...
  11. doi request reprint Novel peptide therapeutics for treatment of infections
    P C F Oyston
    Microbiology, Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Med Microbiol 58:977-87. 2009
    ....
  12. doi request reprint Francisella tularensis vaccines
    Petra C F Oyston
    Biomedical Sciences, Dstl Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK
    Vaccine 27:D48-51. 2009
    ..Alternative approaches to produce subunit vaccines and defined attenuated strains are also in progress. However, the limitations of animal models may make licensing a candidate vaccine challenging...
  13. doi request reprint Q fever: the neglected biothreat agent
    P C F Oyston
    Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Med Microbiol 60:9-21. 2011
    ..burnetii: the first axenic growth medium capable of supporting cell-free growth, and the production of the first isogenic mutant. We are approaching an exciting time for expanding our knowledge of this organism in the next few years...
  14. ncbi request reprint The many and varied niches occupied by Yersinia pestis as an arthropod-vectored zoonotic pathogen
    Petra C F Oyston
    Microbiology, Dstl Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK
    Antonie Van Leeuwenhoek 87:171-7. 2005
    ....
  15. ncbi request reprint Polysaccharides and virulence of Burkholderia pseudomallei
    M Sarkar-Tyson
    Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK
    J Med Microbiol 56:1005-10. 2007
    ..However, immunization with killed polysaccharide mutant strains conferred different degrees of protection, demonstrating the immunological importance of the polysaccharide clusters on the surface of B. pseudomallei...
  16. ncbi request reprint Oral immunization with a dam mutant of Yersinia pseudotuberculosis protects against plague
    Victoria L Taylor
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    Microbiology 151:1919-26. 2005
    ..BALB/c mice inoculated with the mutant were protected against a subcutaneous challenge with 100 MLDs of Yersinia pestis strain GB and an intravenous challenge with 300 MLDs of Y. pseudotuberculosis IP32953...
  17. ncbi request reprint A Francisella tularensis subspecies novicida purF mutant, but not a purA mutant, induces protective immunity to tularemia in mice
    J E Quarry
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 OJQ, UK
    Vaccine 25:2011-8. 2007
    ..tularensis should be further evaluated as live attenuated vaccines against tularemia, but that differential effects are seen depending on which step in the biosynthetic pathway is inactivated...
  18. pmc Protecting against plague: towards a next-generation vaccine
    E D Williamson
    Biomedical Sciences Department, Defence Science and Technology Laboratory, Salisbury, Wilts, UK
    Clin Exp Immunol 172:1-8. 2013
    ..pestis, in order to develop a greater understanding of the protective immune responses required to protect against plague...
  19. ncbi request reprint Protection afforded against aerosol challenge by systemic immunisation with inactivated Francisella tularensis live vaccine strain (LVS)
    J E Eyles
    Defence Science and Technology Laboratory, Porton Down, Salisbury, UK
    Microb Pathog 44:164-8. 2008
    ..These data indicate that parenterally administered non-living vaccines can be used for effective immunisation against aerosol challenges with subspecies holarctica, although not high virulence strains of F. tularensis...
  20. doi request reprint RelA regulates virulence and intracellular survival of Francisella novicida
    R E Dean
    Biomedical Sciences, Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    Microbiology 155:4104-13. 2009
    ..Therefore, (p)ppGpp appears to be an important intracellular signal, integral to the pathogenesis of F. novicida...
  21. doi request reprint Deletion of the Bacillus anthracis capB homologue in Francisella tularensis subspecies tularensis generates an attenuated strain that protects mice against virulent tularaemia
    Stephen L Michell
    Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Med Microbiol 59:1275-84. 2010
    ..These data have important implications for the development of a defined and efficacious tularaemia vaccine...
  22. ncbi request reprint Passive protection against Burkholderia pseudomallei infection in mice by monoclonal antibodies against capsular polysaccharide, lipopolysaccharide or proteins
    S M Jones
    Microbiology, Dstl, CBS Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Med Microbiol 51:1055-62. 2002
    ..However, at a higher B. pseudomallei challenge level of 10(6) cfu none of the MAbs afforded protection and only the anti-exopolysaccharide MAbs produced a significantly delayed time to death...
  23. pmc Pathogenesis of Yersinia pestis infection in BALB/c mice: effects on host macrophages and neutrophils
    Roman A Lukaszewski
    Department of Biomedical Sciences, Dstl Porton Down, Salisbury, Wiltshire, SP4 0JQ, United Kingdom
    Infect Immun 73:7142-50. 2005
    ..pestis is able to grow and escape during the later stages of infection. This macrophage compliance can be overcome in vitro by stimulation with a combination of gamma interferon and tumor necrosis factor alpha...
  24. ncbi request reprint A vaccine for tularaemia
    R W Titball
    Microbiology, Dstl Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK
    Expert Opin Biol Ther 3:645-53. 2003
    ..The approaches which are currently being considered include the production of subunit vaccines and the development of defined isogenic attenuated mutant strains of F. tularensis...
  25. doi request reprint An intracellularly inducible gene involved in virulence and polyphosphate production in Francisella
    Mark I Richards
    Microbiology, Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    J Med Microbiol 57:1183-92. 2008
    ..The mutants exhibited defects for intracellular growth in macrophages and were attenuated in mice, indicating a key role for the putative polyphosphate kinase in the virulence of Francisella...
  26. pmc Tularemia
    Jill Ellis
    Defence Science and Technology Laboratory, CBS Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom
    Clin Microbiol Rev 15:631-46. 2002
    ..tularensis. In the absence of a licensed vaccine, aminoglycoside antibiotics play a key role in the prevention and treatment of tularemia...
  27. ncbi request reprint A dam mutant of Yersinia pestis is attenuated and induces protection against plague
    Victoria L Robinson
    Defence Science and Technology Laboratory, Biomedical Sciences, Salisbury, Wilts, UK
    FEMS Microbiol Lett 252:251-6. 2005
    ..These results confirm the key role of Dam in the regulation of virulence, and its potential role as a target for the generation of attenuated strains of pathogenic bacteria...
  28. ncbi request reprint Animal usage in vaccine development and production: maximising results while minimizing use
    Martin P Smith
    Dstl Biomedical Sciences, CBS Porton Down, Salisbury, SP4OJQ, Wiltshire, UK
    Trends Microbiol 10:62-4. 2002
    ....
  29. doi request reprint Potential of the beta-glucans to enhance innate resistance to biological agents
    Iain J Thompson
    Defence Science and Technology Laboratory, Porton Down, Wiltshire, SP4 OJQ, UK
    Expert Rev Anti Infect Ther 8:339-52. 2010
    ..Overall, the benefits of dietary supplementation with beta-glucans in order to enhance innate resistance to biological agents are evaluated...
  30. ncbi request reprint A 55 kDa hypothetical membrane protein is an iron-regulated virulence factor of Francisella tularensis subsp. novicida U112
    Timothy S Milne
    Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK
    J Med Microbiol 56:1268-76. 2007
    ..Furthermore, the deltaFTT0025 mutant was attenuated in a BALB/c mouse model of infection relative to wild-type F. tularensis subsp. novicida U112...
  31. ncbi request reprint The identification of surface proteins of Burkholderia pseudomallei
    Sarah V Harding
    Dstl, Porton Down, Salisbury, Wiltshire SP4 0JQ, UK
    Vaccine 25:2664-72. 2007
    ..Nine of these proteins were identified by both methods indicating that they may be both surface located and immunogenic: these proteins will be evaluated further as vaccine candidates and diagnostic targets...
  32. ncbi request reprint Immunodominant Francisella tularensis antigens identified using proteome microarray
    Jim E Eyles
    Defence Science and Technology Laboratory, Porton Down, Salisbury, UK
    Proteomics 7:2172-83. 2007
    ..These data give insight into the protective immune response and have potentially important implications for the rational design of non-living vaccines for tularemia and other intracellular pathogens...
  33. ncbi request reprint A liquid-based method for the assessment of bacterial pathogenicity using the nematode Caenorhabditis elegans
    Martin P Smith
    Dstl, Chemical and Biological Sciences, Porton Down, Salisbury SP4 OJQ, UK
    FEMS Microbiol Lett 210:181-5. 2002
    ..Using this assay,P. aeruginosa PA01 inhibited the feeding of C. elegans mutant phm-2. Such liquid-based feeding assays are ideally suited to the high-throughput screening of mutants of bacterial pathogens...
  34. ncbi request reprint Francisella tularensis vaccines
    Kate F Griffin
    Defence Science and Technology Laboratory, Wiltshire, UK
    FEMS Immunol Med Microbiol 49:315-23. 2007
    ..The prospects for a killed whole-cell subunit of live attenuated vaccine are reviewed. A rationally attenuated mutant seems the most likely route to a new tularaemia vaccine...
  35. pmc The immunologically distinct O antigens from Francisella tularensis subspecies tularensis and Francisella novicida are both virulence determinants and protective antigens
    Rebecca M Thomas
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, SP4 0JQ, United Kingdom
    Infect Immun 75:371-8. 2007
    ..The O antigen of F. tularensis subsp. tularensis appeared to be important for intracellular survival whereas the O antigen of F. novicida appeared to be critical for serum resistance and less important for intracellular survival...
  36. ncbi request reprint Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis
    Timothy Atkins
    Defence Science and Technology Laboratory, Salisbury, Wiltshire
    J Med Microbiol 51:539-47. 2002
    ..Mice were dosed with the mutant and subsequently challenged with wild-type B. pseudomallei, but the mutant failed to induce a protective immune response...
  37. doi request reprint The natural history and incidence of Yersinia pestis and prospects for vaccination
    E D Williamson
    Biomedical Sciences, Dstl Porton Down, Salisbury SP4 0JQ, UK
    J Med Microbiol 61:911-8. 2012
    ..The considerable challenges in achieving a vaccine which is licensed for human use and which will comprehensively protect against this serious human pathogen are assessed...
  38. pmc A mutant of Burkholderia pseudomallei, auxotrophic in the branched chain amino acid biosynthetic pathway, is attenuated and protective in a murine model of melioidosis
    T Atkins
    Defence Science and Technology Laboratory, Salisbury, Wiltshire SP4 OJQ, United Kingdom
    Infect Immun 70:5290-4. 2002
    ..Mice inoculated intraperitoneally with the auxotrophic mutant, 35 days prior to challenge, were protected against a challenge dose of 6,000 median lethal doses of wild-type B. pseudomallei...
  39. pmc Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei
    Matthew T G Holden
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 101:14240-5. 2004
    ..mallei. We propose that variable horizontal gene acquisition by B. pseudomallei is an important feature of recent genetic evolution and that this has resulted in a genetically diverse pathogenic species...
  40. ncbi request reprint Structural characterization of lipo-oligosaccharide (LOS) from Yersinia pestis: regulation of LOS structure by the PhoPQ system
    Paul G Hitchen
    Department of Biological Sciences, Wolfson Building, Imperial College, London, SW7 2AY, UK
    Mol Microbiol 44:1637-50. 2002
    ..Thus, LOS modification is under the control of the PhoPQ regulatory system and the ability to alter LOS structure may be required for survival of Y. pestis within the mammalian and/or flea host...
  41. ncbi request reprint DNA adenine methylation
    Victoria L Taylor
    Adv Exp Med Biol 529:13-7. 2003
  42. ncbi request reprint Direct repeat-mediated deletion of a type IV pilin gene results in major virulence attenuation of Francisella tularensis
    Anna Lena Forslund
    Department of Medical Countermeasures, Division of NBC Defence, Swedish Defence Research Agency, Umea
    Mol Microbiol 59:1818-30. 2006
    ..Our findings indicate that this putative pilin is critical for Francisella infections that occur via peripheral routes...
  43. ncbi request reprint Phenotypic characterization of a virulence-associated protein, VagH, of Yersinia pseudotuberculosis reveals a tight link between VagH and the type III secretion system
    Sara Garbom
    Department of Molecular Biology, Umea University, S 90187 Umea, Sweden
    Microbiology 153:1464-73. 2007
    ..In conclusion, the vagH mutant exhibits a virulence phenotype similar to that of a T3SS-negative mutant, indicating a tight link between VagH and type III secretion in Y. pseudotuberculosis...
  44. ncbi request reprint Vaccines against Francisella tularensis
    J Wayne Conlan
    National Research Council Canada, Institute for Biological Sciences, Ottawa, On K1C 2M7, Canada
    Ann N Y Acad Sci 1105:325-50. 2007
    ..These issues and possible solutions are highlighted, along with more modern molecular approaches to vaccine development against this highly virulent pathogen...
  45. ncbi request reprint A plague upon fibrin
    Richard W Titball
    Nat Med 13:253-4. 2007
  46. pmc Application of DNA microarrays to study the evolutionary genomics of Yersinia pestis and Yersinia pseudotuberculosis
    Stewart J Hinchliffe
    The London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK
    Genome Res 13:2018-29. 2003
    ..pseudotuberculosis strains. This study reports chromosomal differences between species, biovars, serotypes, and strains of Y. pestis and Y. pseudotuberculosis that may relate to the evolution of these species in their respective niches...
  47. ncbi request reprint The complete genome sequence of Francisella tularensis, the causative agent of tularemia
    Par Larsson
    Swedish Defence Research Agency, SE 901 82 Umea, Sweden
    Nat Genet 37:153-9. 2005
    ..tularensis is an obligate host-dependent bacterium in its natural life cycle. Our results have implications for our understanding of how highly virulent human pathogens evolve and will expedite strategies to combat them...
  48. ncbi request reprint Signature-tagged mutagenesis of Yersinia pestis
    Petra C F Oyston
    London School of Hygiene and Tropical Medicine, University of London
    Adv Exp Med Biol 529:39-41. 2003