James Sharpe

Summary

Affiliation: National Institute for Medical Research
Country: UK

Publications

  1. ncbi request reprint Optical projection tomography as a tool for 3D microscopy and gene expression studies
    James Sharpe
    Medical Research Council, Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK
    Science 296:541-5. 2002
  2. pmc 3 dimensional modelling of early human brain development using optical projection tomography
    Janet Kerwin
    Institute of Human Genetics, University of Newcastle upon Tyne, International Centre for Life, Central Parkway, Newcastle upon Tyne, NE1 3BZ, UK
    BMC Neurosci 5:27. 2004
  3. ncbi request reprint 3D modelling, gene expression mapping and post-mapping image analysis in the developing human brain
    Subrot Sarma
    Institute of Human Genetics, University of Newcastle, Central Parkway, UK
    Brain Res Bull 66:449-53. 2005
  4. pmc Visualizing plant development and gene expression in three dimensions using optical projection tomography
    Karen Lee
    Department of Cell and Developmental Biology, John Ines Centre, Norwich Research Park, Norwich, NR4 7UH United Kingdom
    Plant Cell 18:2145-56. 2006
  5. ncbi request reprint Tomographic molecular imaging and 3D quantification within adult mouse organs
    Tomas Alanentalo
    Umeå Center for Molecular Medicine, Umea University, S 901 87, Umea, Sweden
    Nat Methods 4:31-3. 2007
  6. pmc Optical projection tomography as a new tool for studying embryo anatomy
    James Sharpe
    Developmental Genetics, MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK
    J Anat 202:175-81. 2003
  7. ncbi request reprint Optical projection tomography
    James Sharpe
    MRC Human Genetics Unit, Western General Hospital, Crewe Road South, EH4 2XU, United Kingdom
    Annu Rev Biomed Eng 6:209-28. 2004
  8. ncbi request reprint EMAP and EMAGE: a framework for understanding spatially organized data
    Richard A Baldock
    MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, UK
    Neuroinformatics 1:309-25. 2003
  9. pmc Three-dimensional imaging of Drosophila melanogaster
    Leeanne McGurk
    Medical Research Council Human Genetics Unit, Edinburgh, United Kingdom
    PLoS ONE 2:e834. 2007
  10. doi request reprint In vitro whole-organ imaging: 4D quantification of growing mouse limb buds
    Marit J Boot
    Medical Research Council Human Genetics Unit, Crewe Road South, Edinburgh EH4 2XU, UK
    Nat Methods 5:609-12. 2008

Collaborators

Detail Information

Publications16

  1. ncbi request reprint Optical projection tomography as a tool for 3D microscopy and gene expression studies
    James Sharpe
    Medical Research Council, Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK
    Science 296:541-5. 2002
    ..OPT microscopy allows the rapid mapping of the tissue distribution of RNA and protein expression in intact embryos or organ systems and can therefore be instrumental in studies of developmental biology or gene function...
  2. pmc 3 dimensional modelling of early human brain development using optical projection tomography
    Janet Kerwin
    Institute of Human Genetics, University of Newcastle upon Tyne, International Centre for Life, Central Parkway, Newcastle upon Tyne, NE1 3BZ, UK
    BMC Neurosci 5:27. 2004
    ..We have assessed the resolution of unstained neuronal structures within a Carnegie Stage (CS)17 OPT model and tested its use as a framework onto which anatomical structures can be defined and gene expression data mapped...
  3. ncbi request reprint 3D modelling, gene expression mapping and post-mapping image analysis in the developing human brain
    Subrot Sarma
    Institute of Human Genetics, University of Newcastle, Central Parkway, UK
    Brain Res Bull 66:449-53. 2005
    ....
  4. pmc Visualizing plant development and gene expression in three dimensions using optical projection tomography
    Karen Lee
    Department of Cell and Developmental Biology, John Ines Centre, Norwich Research Park, Norwich, NR4 7UH United Kingdom
    Plant Cell 18:2145-56. 2006
    ..We also describe tools and software that allow the 3D data to be readily quantified and visualized interactively in different ways...
  5. ncbi request reprint Tomographic molecular imaging and 3D quantification within adult mouse organs
    Tomas Alanentalo
    Umeå Center for Molecular Medicine, Umea University, S 901 87, Umea, Sweden
    Nat Methods 4:31-3. 2007
    ..Using the nonobese diabetic (NOD) mouse model, we demonstrate a correlation between total islet beta-cell volume and the onset of type-1 diabetes...
  6. pmc Optical projection tomography as a new tool for studying embryo anatomy
    James Sharpe
    Developmental Genetics, MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK
    J Anat 202:175-81. 2003
    ..This becomes particularly important for the visualization of the 3D shapes of specific organs and tissues as it allows the computer to automatically determine the outline of the desired structure...
  7. ncbi request reprint Optical projection tomography
    James Sharpe
    MRC Human Genetics Unit, Western General Hospital, Crewe Road South, EH4 2XU, United Kingdom
    Annu Rev Biomed Eng 6:209-28. 2004
    ....
  8. ncbi request reprint EMAP and EMAGE: a framework for understanding spatially organized data
    Richard A Baldock
    MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, UK
    Neuroinformatics 1:309-25. 2003
    ....
  9. pmc Three-dimensional imaging of Drosophila melanogaster
    Leeanne McGurk
    Medical Research Council Human Genetics Unit, Edinburgh, United Kingdom
    PLoS ONE 2:e834. 2007
    ..The data is displayed as 2D optical sections and also in 3D to provide detail on the shape and structure of the adult anatomy...
  10. doi request reprint In vitro whole-organ imaging: 4D quantification of growing mouse limb buds
    Marit J Boot
    Medical Research Council Human Genetics Unit, Crewe Road South, Edinburgh EH4 2XU, UK
    Nat Methods 5:609-12. 2008
    ..We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT)...
  11. ncbi request reprint Correction of artefacts in optical projection tomography
    Johnathon R Walls
    Mouse Imaging Centre, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
    Phys Med Biol 50:4645-65. 2005
    ..Implementation of these corrections and modifications increases the accuracy of the OPT imaging technique and extends its capabilities to obtain higher resolution data from within a whole specimen...
  12. pmc 3D representation of Wnt and Frizzled gene expression patterns in the mouse embryo at embryonic day 11.5 (Ts19)
    Kristen Summerhurst
    Department of Zoology, School of Natural Sciences, Trinity College Dublin, College Green, Dublin, Ireland
    Gene Expr Patterns 8:331-48. 2008
    ..It represents a core set of data that will be extended with additional stages of development and through addition of potentially interacting genes and ultimately other cross-regulatory communication pathways operating in the embryo...
  13. pmc FishNet: an online database of zebrafish anatomy
    Robert J Bryson-Richardson
    The Victor Chang Cardiac Research Institute, 384 Victoria Street, Darlinghurst, Sydney, 2010, Australia
    BMC Biol 5:34. 2007
    ....
  14. ncbi request reprint Resolution improvement in emission optical projection tomography
    Johnathon R Walls
    Mouse Imaging Centre, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON M5G 1X8, Canada
    Phys Med Biol 52:2775-90. 2007
    ..As an illustration, the visibility of fine details in the vasculature of a 9.5 day old mouse embryo is dramatically improved...
  15. pmc Spleen versus pancreas: strict control of organ interrelationship revealed by analyses of Bapx1-/- mice
    Amir Asayesh
    Umeå Centre for Molecular Medicine, Umea University, S 901 87, Umea, Sweden
    Genes Dev 20:2208-13. 2006
    ....
  16. ncbi request reprint Cell tracing reveals a dorsoventral lineage restriction plane in the mouse limb bud mesenchyme
    Carlos G Arques
    Departamento de Biología del Desarrollo Cardiovascular, Centro Nacional de Investigaciones Cardiovasculares, Instituto de Salud Carlos III, E 28029 Madrid, Spain
    Development 134:3713-22. 2007
    ..No lineage compartmentalization was found along the proximodistal or anteroposterior axes, indicating that patterning along these axes does not involve restriction of cell dispersion at specific axial positions...