Colin Longstaff

Summary

Affiliation: National Institute for Biological Standards and Control
Country: UK

Publications

  1. ncbi request reprint A collaborative study to establish the 3rd International Standard for tissue plasminogen activator
    Dawn Sands
    Informatics Laboratory, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    Thromb Haemost 88:294-7. 2002
  2. pmc Kinetic regulation of the binding of prothrombin to phospholipid membranes
    Emma Smith
    Division of Cardiovascular and Diabetes Research, The LIGHT Laboratories, University of Leeds, Leeds, UK
    Mol Cell Biochem 382:193-201. 2013
  3. doi request reprint How do we assure the quality of biological medicines?
    Colin Longstaff
    Division of Biotherapeutics Section, National Institute for Biological Standards and Control, Blanche Lane, S Mimms, Herts EN6 3QG, UK
    Drug Discov Today 14:50-5. 2009
  4. ncbi request reprint Fibrin binding and the regulation of plasminogen activators during thrombolytic therapy
    C Longstaff
    Biotherapeutics Division, NIBSC, S Mimms, Herts, EN6 3QG, UK
    Cardiovasc Hematol Agents Med Chem 6:212-23. 2008
  5. ncbi request reprint An international collaborative study to investigate a proposed reference method for the determination of potency measurements of fibrinolytics in absolute units
    C Longstaff
    Haemostasis Section, National Institute for Biological Standards and Control, South Mimms, UK
    J Thromb Haemost 5:412-4. 2007
  6. ncbi request reprint Understanding the enzymology of fibrinolysis and improving thrombolytic therapy
    Colin Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Herts, EN6 3QG, UK
    FEBS Lett 579:3303-9. 2005
  7. ncbi request reprint An international collaborative study to replace the 1st international standard for prekallikrein activator
    C Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Herts, UK
    Vox Sang 88:143-51. 2005
  8. ncbi request reprint A proposed reference method for plasminogen activators that enables calculation of enzyme activities in SI units
    C Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    J Thromb Haemost 2:1416-21. 2004
  9. ncbi request reprint Characterization of cell-associated plasminogen activation catalyzed by urokinase-type plasminogen activator, but independent of urokinase receptor (uPAR, CD87)
    C Longstaff
    The National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK
    Blood 93:3839-46. 1999
  10. ncbi request reprint Plasminogen activation on the cell surface
    Colin Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Herts UK, EN6 3QG
    Front Biosci 7:d244-55. 2002

Collaborators

Detail Information

Publications24

  1. ncbi request reprint A collaborative study to establish the 3rd International Standard for tissue plasminogen activator
    Dawn Sands
    Informatics Laboratory, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    Thromb Haemost 88:294-7. 2002
    ..The standard was established by WHO in November 2000...
  2. pmc Kinetic regulation of the binding of prothrombin to phospholipid membranes
    Emma Smith
    Division of Cardiovascular and Diabetes Research, The LIGHT Laboratories, University of Leeds, Leeds, UK
    Mol Cell Biochem 382:193-201. 2013
    ..Existing data suggest that the weaker rapid interaction between prothrombin and membranes is the most important in vivo when considering the activation of prothrombin at the cell surface...
  3. doi request reprint How do we assure the quality of biological medicines?
    Colin Longstaff
    Division of Biotherapeutics Section, National Institute for Biological Standards and Control, Blanche Lane, S Mimms, Herts EN6 3QG, UK
    Drug Discov Today 14:50-5. 2009
    ..This review includes historical and regulatory background and three examples of problems seen with biotherapeutics: streptokinase, heparin and TGN1412...
  4. ncbi request reprint Fibrin binding and the regulation of plasminogen activators during thrombolytic therapy
    C Longstaff
    Biotherapeutics Division, NIBSC, S Mimms, Herts, EN6 3QG, UK
    Cardiovasc Hematol Agents Med Chem 6:212-23. 2008
    ....
  5. ncbi request reprint An international collaborative study to investigate a proposed reference method for the determination of potency measurements of fibrinolytics in absolute units
    C Longstaff
    Haemostasis Section, National Institute for Biological Standards and Control, South Mimms, UK
    J Thromb Haemost 5:412-4. 2007
  6. ncbi request reprint Understanding the enzymology of fibrinolysis and improving thrombolytic therapy
    Colin Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Herts, EN6 3QG, UK
    FEBS Lett 579:3303-9. 2005
    ..We have developed precise in vitro methods and with the application of computer simulations, we hope to improve our understanding of plasminogen activation to facilitate improvements in thrombolytic therapy...
  7. ncbi request reprint An international collaborative study to replace the 1st international standard for prekallikrein activator
    C Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Herts, UK
    Vox Sang 88:143-51. 2005
    ..The new standards were freeze dried 20% albumin solution containing PKA, the same solution that is tested using these standards. Aspects of the methodology for PKA determination were also examined as part of this study...
  8. ncbi request reprint A proposed reference method for plasminogen activators that enables calculation of enzyme activities in SI units
    C Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    J Thromb Haemost 2:1416-21. 2004
    ..The method would make a suitable reference method for PAs and provides a rigorous means of studying and modeling the enzymology of fibrinolysis and will be helpful in the rational design of third generation thrombolytic agents...
  9. ncbi request reprint Characterization of cell-associated plasminogen activation catalyzed by urokinase-type plasminogen activator, but independent of urokinase receptor (uPAR, CD87)
    C Longstaff
    The National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK
    Blood 93:3839-46. 1999
    ..This mechanism is likely to operate under conditions commonly used for in vitro studies and may have some significance in vivo...
  10. ncbi request reprint Plasminogen activation on the cell surface
    Colin Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Herts UK, EN6 3QG
    Front Biosci 7:d244-55. 2002
    ....
  11. pmc The interplay between tissue plasminogen activator domains and fibrin structures in the regulation of fibrinolysis: kinetic and microscopic studies
    Colin Longstaff
    National Institute for Biological Standards and Control, South Mimms, UK
    Blood 117:661-8. 2011
    ..Thus, the regulation of fibrinolysis depends on the starting nature of fibrin fibers and complex dynamic interaction between tPA and fibrin structures that vary over time...
  12. pmc Mechanical stability and fibrinolytic resistance of clots containing fibrin, DNA, and histones
    Colin Longstaff
    Biotherapeutics, Haemostasis Section, National Institute for Biological Standards and Control, South Mimms, Potters Bar EN6 3QG, United Kingdom
    J Biol Chem 288:6946-56. 2013
    ....
  13. ncbi request reprint The regulation by fibrinogen and fibrin of tissue plasminogen activator kinetics and inhibition by plasminogen activator inhibitor 1
    C Thelwell
    National Institute for Biological Standards and Control, South Mimms, Herts, UK
    J Thromb Haemost 5:804-11. 2007
    ....
  14. ncbi request reprint International collaborative study to establish the 3rd International Standard for Streptokinase
    D Sands
    Informatics Division, National Institute for Biological Standards and Control, Potters Bar, UK
    J Thromb Haemost 2:1411-5. 2004
    ....
  15. ncbi request reprint Differences between neonates and adults in tissue-type-plasminogen activator (t-PA)-catalyzed plasminogen activation with various effectors and in carbohydrate sequences of fibrinogen chains
    M Ries
    Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK
    Thromb Res 103:173-84. 2001
    ..In contrast to previous data, we conclude that catalytic efficiency of t-PA-catalyzed plasminogen activation in neonates is only slightly lower than in adults...
  16. ncbi request reprint A reunification of the US ("NIH") and International Unit into a single standard for Thrombin
    Colin Whitton
    Division of Haematology, National Institute for Biological Standards and Control, Herts, UK
    Thromb Haemost 93:261-6. 2005
    ..Sample D was adopted as the IS for Thrombin (01/580) and the US Standard (lot K) with a potency of 110 IU/ampoule...
  17. ncbi request reprint Collaborative study to establish a new biological reference preparation for prekallikrein activator
    C Longstaff
    National Institute for Biological Standards and Control, Division of Haematology, South Mimms, Potters Bar, Herts, EN6 3QG United Kingdom
    Pharmeuropa Bio 2005:1-12. 2005
    ..Detailed investigations conducted in this study show that the PKA in albumin used to make samples B and C is very stable and suitable for long-term storage as a reference material...
  18. ncbi request reprint Differences between neonates and adults in carbohydrate sequences and reaction kinetics of plasmin and alpha(2)-antiplasmin
    Martin Ries
    Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK
    Thromb Res 105:247-56. 2002
    ..These findings are important for the understanding of the physiology of the fibrinolytic system in neonates and provide further evidence that differences in glycosylation could be associated with marked effects on protein function...
  19. doi request reprint Release of proteolytic activity following reduction in therapeutic human serum albumin containing products: detection with a new neoepitope endopeptidase immunoassay
    Russell G A Jones
    National Institute for Biological Standards and Control, Health Protection Agency, Division of Bacteriology, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    J Pharm Biomed Anal 54:74-80. 2011
    ..Taken together, these findings indicate a new intrinsic proteolytic activity of the albumin molecule revealed under reducing conditions as the source of the unexpected Arg-Ala cleaving activity...
  20. doi request reprint Commentary: biological standardisation in haemostasis and haematology
    Colin Longstaff
    Biotherapeutics, Haemostasis Section, National Institute for Biological Standards and Control, South Mimms EN63QG, UK
    Biologicals 38:421-2. 2010
    ....
  21. ncbi request reprint Inhibition of cell surface mediated plasminogen activation by a monoclonal antibody against alpha-Enolase
    Roser Lopez-Alemany
    Centre d Oncologia Molecular C O M, Institut de Recerca Oncolgica IRO, Barcelona, Spain
    Am J Hematol 72:234-42. 2003
    ..These data suggest that, although leukocytic cells display several molecules that bind plasminogen, alpha-enolase is responsible for the majority of the promotion of plasminogen activation on the surfaces of leukocytic cells...
  22. ncbi request reprint Phospholipid barrier to fibrinolysis: role for the anionic polar head charge and the gel phase crystalline structure
    Balázs Váradi
    Department of Medical Biochemistry, Semmelweis University, 1088 Budapest, Hungary
    J Biol Chem 279:39863-71. 2004
    ..These findings show a phospholipid barrier, which should be overcome during lysis of arterial thrombi...
  23. ncbi request reprint Regulatory frameworks in developing countries
    Colin Longstaff
    Nat Biotechnol 23:413; author reply 413-4. 2005
  24. ncbi request reprint Kinetics of the inhibition of fusarium serine proteinases by barley (Hordeum vulgare L.) inhibitors
    Anja I Pekkarinen
    Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA
    J Agric Food Chem 55:2736-42. 2007
    ..Understanding the inhibition mechanisms will be valuable in designing improved strategies for increasing the resistance of the grains to fungal infections...