Research Topics
| Colin LongstaffSummaryAffiliation: National Institute for Biological Standards and Control Country: UK Publications
| Collaborators
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Detail Information
Publications
A collaborative study to establish the 3rd International Standard for tissue plasminogen activatorDawn Sands
Informatics Laboratory, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
Thromb Haemost 88:294-7. 2002..The standard was established by WHO in November 2000...- Characterization of cell-associated plasminogen activation catalyzed by urokinase-type plasminogen activator, but independent of urokinase receptor (uPAR, CD87)C Longstaff
The National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK
Blood 93:3839-46. 1999..This mechanism is likely to operate under conditions commonly used for in vitro studies and may have some significance in vivo... - How do we assure the quality of biological medicines?Colin Longstaff
Division of Biotherapeutics Section, National Institute for Biological Standards and Control, Blanche Lane, S Mimms, Herts EN6 3QG, UK
Drug Discov Today 14:50-5. 2009..This review includes historical and regulatory background and three examples of problems seen with biotherapeutics: streptokinase, heparin and TGN1412... - Plasminogen activation on the cell surfaceColin Longstaff
Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Herts UK, EN6 3QG
Front Biosci 7:d244-55. 2002.... - Fibrin binding and the regulation of plasminogen activators during thrombolytic therapyC Longstaff
Biotherapeutics Division, NIBSC, S Mimms, Herts, EN6 3QG, UK
Cardiovasc Hematol Agents Med Chem 6:212-23. 2008.... - An international collaborative study to investigate a proposed reference method for the determination of potency measurements of fibrinolytics in absolute unitsC Longstaff
Haemostasis Section, National Institute for Biological Standards and Control, South Mimms, UK
J Thromb Haemost 5:412-4. 2007 - Understanding the enzymology of fibrinolysis and improving thrombolytic therapyColin Longstaff
Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Herts, EN6 3QG, UK
FEBS Lett 579:3303-9. 2005..We have developed precise in vitro methods and with the application of computer simulations, we hope to improve our understanding of plasminogen activation to facilitate improvements in thrombolytic therapy... - An international collaborative study to replace the 1st international standard for prekallikrein activatorC Longstaff
Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Herts, UK
Vox Sang 88:143-51. 2005..The new standards were freeze dried 20% albumin solution containing PKA, the same solution that is tested using these standards. Aspects of the methodology for PKA determination were also examined as part of this study... - A proposed reference method for plasminogen activators that enables calculation of enzyme activities in SI unitsC Longstaff
Division of Haematology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
J Thromb Haemost 2:1416-21. 2004..The method would make a suitable reference method for PAs and provides a rigorous means of studying and modeling the enzymology of fibrinolysis and will be helpful in the rational design of third generation thrombolytic agents... - The interplay between tissue plasminogen activator domains and fibrin structures in the regulation of fibrinolysis: kinetic and microscopic studiesColin Longstaff
National Institute for Biological Standards and Control, South Mimms, UK
Blood 117:661-8. 2011..Thus, the regulation of fibrinolysis depends on the starting nature of fibrin fibers and complex dynamic interaction between tPA and fibrin structures that vary over time... - International collaborative study to establish the 3rd International Standard for StreptokinaseD Sands
Informatics Division, National Institute for Biological Standards and Control, Potters Bar, UK
J Thromb Haemost 2:1411-5. 2004.... - The regulation by fibrinogen and fibrin of tissue plasminogen activator kinetics and inhibition by plasminogen activator inhibitor 1C Thelwell
National Institute for Biological Standards and Control, South Mimms, Herts, UK
J Thromb Haemost 5:804-11. 2007.... - Differences between neonates and adults in tissue-type-plasminogen activator (t-PA)-catalyzed plasminogen activation with various effectors and in carbohydrate sequences of fibrinogen chainsM Ries
Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK
Thromb Res 103:173-84. 2001..In contrast to previous data, we conclude that catalytic efficiency of t-PA-catalyzed plasminogen activation in neonates is only slightly lower than in adults... - A reunification of the US ("NIH") and International Unit into a single standard for ThrombinColin Whitton
Division of Haematology, National Institute for Biological Standards and Control, Herts, UK
Thromb Haemost 93:261-6. 2005..Sample D was adopted as the IS for Thrombin (01/580) and the US Standard (lot K) with a potency of 110 IU/ampoule... - Collaborative study to establish a new biological reference preparation for prekallikrein activatorC Longstaff
National Institute for Biological Standards and Control, Division of Haematology, South Mimms, Potters Bar, Herts, EN6 3QG United Kingdom
Pharmeuropa Bio 2005:1-12. 2005..Detailed investigations conducted in this study show that the PKA in albumin used to make samples B and C is very stable and suitable for long-term storage as a reference material... - Release of proteolytic activity following reduction in therapeutic human serum albumin containing products: detection with a new neoepitope endopeptidase immunoassayRussell G A Jones
National Institute for Biological Standards and Control, Health Protection Agency, Division of Bacteriology, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
J Pharm Biomed Anal 54:74-80. 2011..Taken together, these findings indicate a new intrinsic proteolytic activity of the albumin molecule revealed under reducing conditions as the source of the unexpected Arg-Ala cleaving activity... - Differences between neonates and adults in carbohydrate sequences and reaction kinetics of plasmin and alpha(2)-antiplasminMartin Ries
Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK
Thromb Res 105:247-56. 2002..These findings are important for the understanding of the physiology of the fibrinolytic system in neonates and provide further evidence that differences in glycosylation could be associated with marked effects on protein function... - Commentary: biological standardisation in haemostasis and haematologyColin Longstaff
Biotherapeutics, Haemostasis Section, National Institute for Biological Standards and Control, South Mimms EN63QG, UK
Biologicals 38:421-2. 2010.... - Inhibition of cell surface mediated plasminogen activation by a monoclonal antibody against alpha-EnolaseRoser Lopez-Alemany
Centre d Oncologia Molecular C O M, Institut de Recerca Oncolgica IRO, Barcelona, Spain
Am J Hematol 72:234-42. 2003..These data suggest that, although leukocytic cells display several molecules that bind plasminogen, alpha-enolase is responsible for the majority of the promotion of plasminogen activation on the surfaces of leukocytic cells... - Kinetics of the inhibition of fusarium serine proteinases by barley (Hordeum vulgare L.) inhibitorsAnja I Pekkarinen
Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA
J Agric Food Chem 55:2736-42. 2007..Understanding the inhibition mechanisms will be valuable in designing improved strategies for increasing the resistance of the grains to fungal infections... - Regulatory frameworks in developing countriesColin Longstaff
Nat Biotechnol 23:413; author reply 413-4. 2005