R G A Jones

Summary

Affiliation: National Institute for Biological Standards and Control
Country: UK

Publications

  1. ncbi request reprint A review of WHO International Standards for botulinum antitoxins
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
    Biologicals 34:223-6. 2006
  2. ncbi request reprint A protocol for 'enhanced pepsin digestion': a step by step method for obtaining pure antibody fragments in high yield from serum
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control NIBSC, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    J Immunol Methods 275:239-50. 2003
  3. ncbi request reprint Botulinum type A toxin neutralisation by specific IgG and its fragments: a comparison of mouse systemic toxicity and local flaccid paralysis assays
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    Toxicon 48:246-54. 2006
  4. ncbi request reprint Development of improved SNAP25 endopeptidase immuno-assays for botulinum type A and E toxins
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    J Immunol Methods 329:92-101. 2008
  5. doi request reprint An improved method for development of toxoid vaccines and antitoxins
    Russell G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    J Immunol Methods 337:42-8. 2008
  6. doi request reprint New highly specific botulinum type C1 endopeptidase immunoassays utilising SNAP25 or Syntaxin substrates
    Russell G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Hertfordshire, UK
    J Immunol Methods 343:21-7. 2009
  7. doi request reprint Phrenic nerve-hemidiaphragm as a highly sensitive replacement assay for determination of functional botulinum toxin antibodies
    C Rasetti-Escargueil
    Division of Bacteriology and Biostatistics Section, National Institute for Biological Standards and Control NIBSC, Health Protection Agency HPA, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    Toxicon 57:1008-16. 2011
  8. doi request reprint Measurement of botulinum types A, B and E neurotoxicity using the phrenic nerve-hemidiaphragm: improved precision with in-bred mice
    C Rasetti-Escargueil
    Division of Bacteriology, National Institute for Biological Standards and Control Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    Toxicon 53:503-11. 2009
  9. ncbi request reprint Detection of antibodies against botulinum toxins
    Dorothea Sesardic
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, United Kingdom
    Mov Disord 19:S85-91. 2004

Collaborators

Detail Information

Publications9

  1. ncbi request reprint A review of WHO International Standards for botulinum antitoxins
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
    Biologicals 34:223-6. 2006
    ..We describe here the production and characterization of stable freeze dried potential candidate replacements along with a new prospective first IS for type G antitoxin. Available toxin A reference preparations are also briefly reviewed...
  2. ncbi request reprint A protocol for 'enhanced pepsin digestion': a step by step method for obtaining pure antibody fragments in high yield from serum
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control NIBSC, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    J Immunol Methods 275:239-50. 2003
    ..If required, a mild reducing agent may be used finally to create Fab fragments...
  3. ncbi request reprint Botulinum type A toxin neutralisation by specific IgG and its fragments: a comparison of mouse systemic toxicity and local flaccid paralysis assays
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    Toxicon 48:246-54. 2006
    ..These findings show that a local flaccid paralysis assay provides a less time consuming and more humane alternative to the lethal assay for the potency testing of botulinum IgG and F(ab')(2) antitoxins...
  4. ncbi request reprint Development of improved SNAP25 endopeptidase immuno-assays for botulinum type A and E toxins
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    J Immunol Methods 329:92-101. 2008
    ..The principle of using a detecting antibody to a substrate sequence buried within the native substrates alpha-helix may be further expanded to other specific enzyme cleavage reactions in the future...
  5. doi request reprint An improved method for development of toxoid vaccines and antitoxins
    Russell G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    J Immunol Methods 337:42-8. 2008
    ..Toxoid produced by the new method should substantially improve both antitoxin and vaccine production and be applicable to other toxins and immunogens...
  6. doi request reprint New highly specific botulinum type C1 endopeptidase immunoassays utilising SNAP25 or Syntaxin substrates
    Russell G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Hertfordshire, UK
    J Immunol Methods 343:21-7. 2009
    ..These new immunoassays will therefore provide highly specific tools for monitoring botulinum toxin light chain endopeptidase activity and serotype identity...
  7. doi request reprint Phrenic nerve-hemidiaphragm as a highly sensitive replacement assay for determination of functional botulinum toxin antibodies
    C Rasetti-Escargueil
    Division of Bacteriology and Biostatistics Section, National Institute for Biological Standards and Control NIBSC, Health Protection Agency HPA, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    Toxicon 57:1008-16. 2011
    ..964, respectively). These findings confirm that the mouse nerve-diaphragm preparation can provide a functional ex vivo replacement assay for specific, sensitive and precise assessment of toxin and antitoxin activity...
  8. doi request reprint Measurement of botulinum types A, B and E neurotoxicity using the phrenic nerve-hemidiaphragm: improved precision with in-bred mice
    C Rasetti-Escargueil
    Division of Bacteriology, National Institute for Biological Standards and Control Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    Toxicon 53:503-11. 2009
    ..These findings indicate that when fully optimised the mouse nerve-diaphragm preparation can provide a functional in vitro model for accurate and reproducible assessment of toxin activity...
  9. ncbi request reprint Detection of antibodies against botulinum toxins
    Dorothea Sesardic
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, United Kingdom
    Mov Disord 19:S85-91. 2004
    ..Most recent promising developments are focused on cellular assays utilising primary rat embryonic cord cells or more conveniently in vitro differentiated established cell lines such as human neuroblastoma cells...