John F Eccleston

Summary

Affiliation: National Institute for Medical Research
Country: UK

Publications

  1. ncbi request reprint The mechanism of Ras GTPase activation by neurofibromin
    Robert A Phillips
    National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
    Biochemistry 42:3956-65. 2003
  2. ncbi request reprint Rapid kinetic techniques
    John F Eccleston
    Division of Physical Biochemistry, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Methods Cell Biol 84:445-77. 2008
  3. ncbi request reprint The kinetic mechanism of the SufC ATPase: the cleavage step is accelerated by SufB
    John F Eccleston
    Medical Research Council National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Biol Chem 281:8371-8. 2006
  4. ncbi request reprint Oligomerization and kinetic mechanism of the dynamin GTPase
    John F Eccleston
    National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Eur Biophys J 31:275-82. 2002
  5. pmc Structure of mammalian AMPK and its regulation by ADP
    Bing Xiao
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 472:230-3. 2011
  6. pmc Hydrodynamic characterization of the SufBC and SufCD complexes and their interaction with fluorescent adenosine nucleotides
    Arsen Petrovic
    MRC National Institute for Medical Research, London NW7 1AA, United Kingdom
    Protein Sci 17:1264-74. 2008
  7. pmc Mechanism of interaction between single-stranded DNA binding protein and DNA
    Simone Kunzelmann
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Biochemistry 49:843-52. 2010
  8. ncbi request reprint Fluorescence-based assays
    John F Eccleston
    National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK
    Prog Med Chem 43:19-48. 2005
  9. ncbi request reprint Structural basis for AMP binding to mammalian AMP-activated protein kinase
    Bing Xiao
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 449:496-500. 2007
  10. pmc Structural analysis reveals an amyloid form of the human papillomavirus type 16 E1--E4 protein and provides a molecular basis for its accumulation
    Pauline B McIntosh
    Division of Virology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Virol 82:8196-203. 2008

Collaborators

Detail Information

Publications17

  1. ncbi request reprint The mechanism of Ras GTPase activation by neurofibromin
    Robert A Phillips
    National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
    Biochemistry 42:3956-65. 2003
    ..In this model, the conformation change on P(i) release causes the large change in affinity of neurofibromin, which then dissociates rapidly...
  2. ncbi request reprint Rapid kinetic techniques
    John F Eccleston
    Division of Physical Biochemistry, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Methods Cell Biol 84:445-77. 2008
    ..We discuss the experimental design appropriate for the different experimental techniques and reaction mechanisms, as well as the fundamental theoretical concepts behind the analysis of the data obtained...
  3. ncbi request reprint The kinetic mechanism of the SufC ATPase: the cleavage step is accelerated by SufB
    John F Eccleston
    Medical Research Council National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Biol Chem 281:8371-8. 2006
    ..In the absence and presence of SufB, mantADP remains more tightly bound to SufC than mantATP. These studies provide a basis for how the SufB and -C proteins interact in the processes involved in regulating iron-sulfur transfer...
  4. ncbi request reprint Oligomerization and kinetic mechanism of the dynamin GTPase
    John F Eccleston
    National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Eur Biophys J 31:275-82. 2002
    ..New data on the interaction of dynamin with a fluorescent derivative of GTPgammaS are also presented. The possible mechanism by which assembly of dynamin leads to an increase in its GTPase activity is discussed...
  5. pmc Structure of mammalian AMPK and its regulation by ADP
    Bing Xiao
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 472:230-3. 2011
    ..From our biochemical and structural data we develop a model for how the energy status of a cell regulates AMPK activity...
  6. pmc Hydrodynamic characterization of the SufBC and SufCD complexes and their interaction with fluorescent adenosine nucleotides
    Arsen Petrovic
    MRC National Institute for Medical Research, London NW7 1AA, United Kingdom
    Protein Sci 17:1264-74. 2008
    ..Given that SufB and SufD have 20% sequence identity and similar predicted secondary structures, the different hydrodynamic properties and kinetic mechanisms of the two complexes are discussed...
  7. pmc Mechanism of interaction between single-stranded DNA binding protein and DNA
    Simone Kunzelmann
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Biochemistry 49:843-52. 2010
    ..1 microM at a high ionic strength (200 mM NaCl). Shorter lengths of ssDNA were tested for binding: only when the length is reduced to 20 bases is the affinity significantly reduced...
  8. ncbi request reprint Fluorescence-based assays
    John F Eccleston
    National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK
    Prog Med Chem 43:19-48. 2005
  9. ncbi request reprint Structural basis for AMP binding to mammalian AMP-activated protein kinase
    Bing Xiao
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 449:496-500. 2007
    ..The structure also suggests a mechanism for propagating AMP/ATP signalling whereby a phosphorylated residue from the alpha and/or beta subunits binds to the gamma subunit in the presence of AMP but not when ATP is bound...
  10. pmc Structural analysis reveals an amyloid form of the human papillomavirus type 16 E1--E4 protein and provides a molecular basis for its accumulation
    Pauline B McIntosh
    Division of Virology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Virol 82:8196-203. 2008
    ..Our results suggest a role for fibril formation in facilitating the accumulation of E1--E4 during HPV infection...
  11. ncbi request reprint Sedimentation equilibrium studies
    Ian A Taylor
    Division of Protein Structure, National Institute for Medical Research, London, England, UK
    Methods Mol Biol 261:119-36. 2004
    ..This technique is independent of the shape of the macromolecule under investigation and allows the determination of equilibrium constants for a monomer-multimer self-associating system...
  12. ncbi request reprint Structural and biochemical characterization of a fluorogenic rhodamine-labeled malarial protease substrate
    Michael J Blackman
    National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, U K
    Biochemistry 41:12244-52. 2002
    ..These data also indicate that the rhodamine dimers fluoresce and that the associated lifetimes are subnanosecond...
  13. pmc The 2'-O- and 3'-O-Cy3-EDA-ATP(ADP) complexes with myosin subfragment-1 are spectroscopically distinct
    Kazuhiro Oiwa
    Kanasi Advanced Research Center, Kobe 651 2492, Japan
    Biophys J 84:634-42. 2003
    ..Thus, when bound, 3'-O-Cy3-EDA-ADP's fluorescence is twofold brighter with motion more restricted and turnover slower than the 2'-O-isomer; these data are relevant for applications of these analogs in single molecule studies...
  14. ncbi request reprint Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging
    Tom Robinson
    Chemical Biology Centre, Department of Chemical Engineering, Imperial College London, London, UK
    Opt Lett 33:1887-9. 2008
    ..Following experimental and CFD optimization, mixing times down to 1.3+/-0.4 ms were achieved with the single-layer microfluidic device...
  15. ncbi request reprint H-NS oligomerization domain structure reveals the mechanism for high order self-association of the intact protein
    Diego Esposito
    Department of Biochemistry and Molecular Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    J Mol Biol 324:841-50. 2002
    ..A model is presented for the structural basis for DNA packaging in bacterial cells...
  16. pmc Cooperative hydrogen bond interactions in the streptavidin-biotin system
    David E Hyre
    Box 351721, Department of Bioengineering, University of Washington, Seattle, WA 98195 1721, USA
    Protein Sci 15:459-67. 2006
    ..Taken together, the thermodynamic and structural analyses support the conclusion that the wild-type hydrogen bond between D128-OD and biotin-N2 is thermodynamically stronger than that between S45-OG and biotin-N1...
  17. ncbi request reprint MgF(3)(-) as a transition state analog of phosphoryl transfer
    Debbie L Graham
    Computational and Structural Sciences, GlaxoSmithKline, Gunnels Wood Road, Stevenage, Herts, United Kingdom
    Chem Biol 9:375-81. 2002
    ..The structure adopted by GDP.MgF(-) possesses the stereochemistry and approximate charge expected for the transition state. We suggest that MgF3(-) may be the reagent of choice for studying phosphoryl transfer reactions...