J Doorbar

Summary

Affiliation: National Institute for Medical Research
Country: UK

Publications

  1. ncbi request reprint Molecular biology of human papillomavirus infection and cervical cancer
    John Doorbar
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Clin Sci (Lond) 110:525-41. 2006
  2. ncbi request reprint Intrabody strategies for the treatment of human papillomavirus-associated disease
    John Doorbar
    National Institute for Medical Research, Division of Virology, The Ridgeway, Mill Hill, London, NW7 1AA, UK
    Expert Opin Biol Ther 7:677-89. 2007
  3. pmc The biology of papillomavirus latency
    Gareth Adam Maglennon
    Pathology and Infectious Diseases, The Royal Veterinary College, North Mymms, AL9 7TA, UK
    Open Virol J 6:190-7. 2012
  4. pmc E4 antibodies facilitate detection and type-assignment of active HPV infection in cervical disease
    Heather Griffin
    National Institute for Medical Research, London, United Kingdom
    PLoS ONE 7:e49974. 2012
  5. doi request reprint The E4 protein; structure, function and patterns of expression
    John Doorbar
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom Electronic address
    Virology 445:80-98. 2013
  6. doi request reprint Latent papillomavirus infections and their regulation
    John Doorbar
    Division of Virology, National Institute for Medical Research, London, United Kingdom
    Curr Opin Virol 3:416-21. 2013
  7. doi request reprint The biology and life-cycle of human papillomaviruses
    John Doorbar
    Division of Virology, National Institute for Medical Research, London, United Kingdom
    Vaccine 30:F55-70. 2012
  8. pmc Persistence of viral DNA in the epithelial basal layer suggests a model for papillomavirus latency following immune regression
    Gareth Adam Maglennon
    Division of Virology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK
    Virology 414:153-63. 2011
  9. pmc Papillomavirus life cycle organization and biomarker selection
    John Doorbar
    National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK
    Dis Markers 23:297-313. 2007
  10. ncbi request reprint The papillomavirus life cycle
    John Doorbar
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    J Clin Virol 32:S7-15. 2005

Collaborators

  • Clare Davy
  • R C Elston
  • Neil Christensen
  • K Sotlar
  • Janet Brandsma
  • Jonathan B A Millar
  • Anco Molijn
  • Ignacio G Bravo
  • Lawrence Banks
  • Nancy M Cladel
  • David Jenkins
  • Nicholas Coleman
  • Xing Wu
  • Kiyofumi Egawa
  • John F Eccleston
  • J Dunlop
  • Qian Wang
  • Heather Griffin
  • Pauline B McIntosh
  • Gareth Adam Maglennon
  • Woei Ling Peh
  • Jameela Khan
  • Deborah J Jackson
  • Peter Laskey
  • Pauline McIntosh
  • G A Maglennon
  • Kenneth Raj
  • Tomomi Nakahara
  • Shirley Southern
  • Heather M Griffin
  • Kate Middleton
  • Erin R Isaacson
  • Deborah Jackson
  • Helena Sterlinko Grm
  • Woei L Peh
  • Merilyn Hibma
  • Kate Matthews
  • P B McIntosh
  • Rebecca Marnane
  • Christine Van Hoof
  • Zhonglin Wu
  • Vincent Dewar
  • Wim Quint
  • Frank Struyf
  • Brigitte Colau
  • Steven Hinz
  • Kate Sullivan
  • Alan Kennedy
  • Steven A Howell
  • Steven A Hinz
  • Papia Das
  • Stephen R Martin
  • Lesley Calder
  • Keith Ansell
  • Greg Winter
  • Michael Coleman
  • Denis Lee
  • Paul F Lambert
  • Malte Weber
  • Phillip J Masterson
  • Samuel Berguerand
  • M Bishr Omary
  • Ana Martin
  • Peter Beard
  • Philip A Walker
  • Rashmi Seth
  • Lindsay Baxter
  • Emma Inglis
  • Woei Peh
  • Cheng Mee Leong
  • Paul Lambert
  • Kankatsu Yun
  • B Thomas Bäckström
  • Lesley Morris
  • Amira El-Sherif
  • Alan Percival
  • Jon Lewis
  • Philip Nicholls
  • Wen Jun Liu

Detail Information

Publications34

  1. ncbi request reprint Molecular biology of human papillomavirus infection and cervical cancer
    John Doorbar
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Clin Sci (Lond) 110:525-41. 2006
    ..In most individuals, immune regression eventually leads to clearance of the virus, or to its maintenance in a latent or asymptomatic state in the basal cells...
  2. ncbi request reprint Intrabody strategies for the treatment of human papillomavirus-associated disease
    John Doorbar
    National Institute for Medical Research, Division of Virology, The Ridgeway, Mill Hill, London, NW7 1AA, UK
    Expert Opin Biol Ther 7:677-89. 2007
    ..The clinical utility of the approach is considered alongside the general difficulties of using protein molecules as intracellular therapeutics...
  3. pmc The biology of papillomavirus latency
    Gareth Adam Maglennon
    Pathology and Infectious Diseases, The Royal Veterinary College, North Mymms, AL9 7TA, UK
    Open Virol J 6:190-7. 2012
    ....
  4. pmc E4 antibodies facilitate detection and type-assignment of active HPV infection in cervical disease
    Heather Griffin
    National Institute for Medical Research, London, United Kingdom
    PLoS ONE 7:e49974. 2012
    ..The unique characteristics of the E4 biomarker suggest a role in diagnosis and patient management particularly when used in combination...
  5. doi request reprint The E4 protein; structure, function and patterns of expression
    John Doorbar
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom Electronic address
    Virology 445:80-98. 2013
    ..Assembly into higher-order multimers and suppression of cell proliferation are common to all E4 proteins examined. Although not yet formally demonstrated, a role in virus release and transmission remains a likely function for E4...
  6. doi request reprint Latent papillomavirus infections and their regulation
    John Doorbar
    Division of Virology, National Institute for Medical Research, London, United Kingdom
    Curr Opin Virol 3:416-21. 2013
    ....
  7. doi request reprint The biology and life-cycle of human papillomaviruses
    John Doorbar
    Division of Virology, National Institute for Medical Research, London, United Kingdom
    Vaccine 30:F55-70. 2012
    ..This article forms part of a special supplement entitled "Comprehensive Control of HPV Infections and Related Diseases" Vaccine Volume 30, Supplement 5, 2012...
  8. pmc Persistence of viral DNA in the epithelial basal layer suggests a model for papillomavirus latency following immune regression
    Gareth Adam Maglennon
    Division of Virology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK
    Virology 414:153-63. 2011
    ..We hypothesize that these cells are epithelial stem cells and that reactivation of latency may be a source of recurrent disease...
  9. pmc Papillomavirus life cycle organization and biomarker selection
    John Doorbar
    National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK
    Dis Markers 23:297-313. 2007
    ....
  10. ncbi request reprint The papillomavirus life cycle
    John Doorbar
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    J Clin Virol 32:S7-15. 2005
    ..As with other DNA tumour viruses, such abortive infections can predispose to cancer...
  11. ncbi request reprint Characterization of events during the late stages of HPV16 infection in vivo using high-affinity synthetic Fabs to E4
    J Doorbar
    National Institute for Medical Research, London, United Kingdom
    Virology 238:40-52. 1997
    ..The Fabs will be of value in the evaluation of model systems for mimicking HPV infection in vitro...
  12. pmc The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus
    J Doorbar
    Division of Virology, National Institute for Medical Research, Mill Hill, London, United Kingdom
    J Virol 74:10081-95. 2000
    ..Identification of E4-DBP as an HPV16 E1E4-associated protein indicates a possible role for E1E4 in virus synthesis...
  13. ncbi request reprint The identification of a conserved binding motif within human papillomavirus type 16 E6 binding peptides, E6AP and E6BP
    R C Elston
    Division of Virology, National Institute for Medical Research, London, UK
    J Gen Virol 79:371-4. 1998
    ..A synthetic peptide containing the ELLG motif blocked the interaction of E6 with both E6-AP and E6-BP. The data suggest that E6 interacts through a structurally similar binding domain present within a number of cellular proteins...
  14. doi request reprint A novel interaction between the human papillomavirus type 16 E2 and E1--E4 proteins leads to stabilization of E2
    Clare Davy
    Division of Virology, MRC National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK
    Virology 394:266-75. 2009
    ..In addition, the turnover of the E2 protein is decreased in the presence of E1--E4. All this raises the possibility that E1--E4 acts to influence E2 activity by varying the amount of available E2 in the cell...
  15. pmc E1--E4-mediated keratin phosphorylation and ubiquitylation: a mechanism for keratin depletion in HPV16-infected epithelium
    Pauline B McIntosh
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, N10 3UE, UK
    J Cell Sci 123:2810-22. 2010
    ....
  16. ncbi request reprint HPV16 E1--E4 protein is phosphorylated by Cdk2/cyclin A and relocalizes this complex to the cytoplasm
    Clare E Davy
    Division of Virology, MRC National Institute for Medical Research, London NW7 1AA, UK
    Virology 349:230-44. 2006
    ..16E1--E4 serine 32 was found to be phosphorylated by Cdk2/cyclin A. We hypothesize that the interaction of 16E1--E4 with cyclin A may serve to increase the efficiency with which 16E1--E4 is able to prevent mitotic entry...
  17. ncbi request reprint Molecular basis for advances in cervical screening
    John Doorbar
    National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK
    Mol Diagn 9:129-42. 2005
    ....
  18. pmc Human papillomavirus type 16 E1 E4-induced G2 arrest is associated with cytoplasmic retention of active Cdk1/cyclin B1 complexes
    Clare E Davy
    Division of Virology, National Institute for Medical Research, London, NW7 1AA, United Kingdom
    J Virol 79:3998-4011. 2005
    ..We hypothesize that E1 E4-induced G2 arrest may play a role in creating an environment optimal for viral DNA replication and that loss of E1 E4 expression may contribute to malignant progression...
  19. pmc Organization of human papillomavirus productive cycle during neoplastic progression provides a basis for selection of diagnostic markers
    Kate Middleton
    National Institute for Medical Research, Mill Hill, London
    J Virol 77:10186-201. 2003
    ..It appears from our work that the presence of such proteins in cells at the epithelial surface allows the severity of the underlying disease to be predicted and that markers of viral gene expression may improve cervical screening...
  20. pmc Functional analysis of the human papillomavirus type 16 E1=E4 protein provides a mechanism for in vivo and in vitro keratin filament reorganization
    Qian Wang
    Division of Virology, National Institute for Medical Research, London, United Kingdom
    J Virol 78:821-33. 2004
    ..The increase in avidity associated with multimeric binding may contribute to the ability of 16E1=E4 to sequester its cellular targets in the cytoplasm...
  21. pmc Immunosuppression facilitates the reactivation of latent papillomavirus infections
    G A Maglennon
    National Institute for Medical Research, Mill Hill, London, United Kingdom
    J Virol 88:710-6. 2014
    ..Our studies provide direct evidence that immunosuppression can trigger the reactivation of latent papillomavirus genomes, as previously proposed in humans. ..
  22. pmc Phosphorylation of the human papillomavirus type 16 E1--E4 protein at T57 by ERK triggers a structural change that enhances keratin binding and protein stability
    Qian Wang
    Division of Virology, MRC National Institute for Medical Research, London NW7 1AA, United Kingdom
    J Virol 83:3668-83. 2009
    ..Other forms of E1--E4, with alternative functional roles, may then increase in prevalence in the upper layers of the epithelium...
  23. pmc Identification of a G(2) arrest domain in the E1 wedge E4 protein of human papillomavirus type 16
    Clare E Davy
    Division of Virology, National Institute for Medical Research, London NW7 1AA
    J Virol 76:9806-18. 2002
    ..We conclude that, like other papillomavirus proteins, 16E1 wedge E4 affects cell cycle progression and that it targets a conserved component of the cell cycle machinery...
  24. pmc Life cycle heterogeneity in animal models of human papillomavirus-associated disease
    Woei Ling Peh
    National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    J Virol 76:10401-16. 2002
    ..The persistence of abortive infections caused by CRPV offers advantages for the development of therapeutic vaccines...
  25. pmc The viral E4 protein is required for the completion of the cottontail rabbit papillomavirus productive cycle in vivo
    Woei L Peh
    Division of Virology, The National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Virol 78:2142-51. 2004
    ..These findings suggest that efficient amplification of the CRPV genome is dependent on the expression of a full-length CRPV E4 protein...
  26. ncbi request reprint Inhibition of papillomavirus protein function in cervical cancer cells by intrabody targeting
    Heather Griffin
    Division of Virology, National Institute for Medical Research, London NW7 1AA, UK
    J Mol Biol 355:360-78. 2006
    ....
  27. ncbi request reprint Detection of papillomavirus proteins and DNA in paraffin-embedded tissue sections
    Woei Ling Peh
    Division of Virology, The National Institute for Medical Research, Mill Hill, London, UK
    Methods Mol Med 119:49-59. 2005
    ..Visualization of both nucleic acid and protein targets can be done simultaneously by combining in situ hybridization and immuno-detection methods...
  28. pmc Role of calpain in the formation of human papillomavirus type 16 E1^E4 amyloid fibers and reorganization of the keratin network
    Jameela Khan
    MRC National Institute for Medical Research, London, UK
    J Virol 85:9984-97. 2011
    ....
  29. pmc Structural analysis reveals an amyloid form of the human papillomavirus type 16 E1--E4 protein and provides a molecular basis for its accumulation
    Pauline B McIntosh
    Division of Virology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    J Virol 82:8196-203. 2008
    ..Our results suggest a role for fibril formation in facilitating the accumulation of E1--E4 during HPV infection...
  30. ncbi request reprint G2/M cell cycle arrest in the life cycle of viruses
    Clare Davy
    Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK
    Virology 368:219-26. 2007
    ..However, a number of effects of arrest are being investigated and it will be interesting to see to what extent perturbation of the G2/M transition is involved in viral infections...
  31. pmc E1 empty set E4 protein of human papillomavirus type 16 associates with mitochondria
    Kenneth Raj
    Swiss Institute for Experimental Cancer Research and NCRR Molecular Oncology, 1066 Epalinges, Switzerland
    J Virol 78:7199-207. 2004
    ....
  32. pmc Depletion of Langerhans cells in human papillomavirus type 16-infected skin is associated with E6-mediated down regulation of E-cadherin
    Kate Matthews
    Virus Research Unit, Department of Microbiology, Malaghan Institute of Medical Research, Wellington School of Medicine, University of Otago, Dunedin, New Zealand
    J Virol 77:8378-85. 2003
    ..Through this pathway, E6 expression in HPV16-infected KC may limit presentation of viral antigens by LC to the immune system, thus preventing the initiation of a cell-mediated immune response and promoting survival of the virus...
  33. pmc Human papillomavirus type 16 E1circumflexE4 contributes to multiple facets of the papillomavirus life cycle
    Tomomi Nakahara
    McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, 53706, USA
    J Virol 79:13150-65. 2005
    ..These data support the hypothesis that E1(circumflex)E4 is a multifunctional protein and that the different properties of E1(circumflex)E4 contribute to different processes in both the early and late stages of the virus life cycle...
  34. ncbi request reprint Inhibition of E6-induced degradation of its cellular substrates by novel blocking peptides
    Helena Sterlinko Grm
    International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I 34012 Trieste, Italy
    J Mol Biol 335:971-85. 2004
    ..These results suggest that the site on E6 bound by E6-AP is also most likely occupied by other, as yet unidentified, ubiquitin ligases...