D Carling

Summary

Affiliation: National Institute for Medical Research
Country: UK

Publications

  1. pmc ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein kinase
    Faith V Mayer
    MRC Clinical Sciences Centre, Cellular Stress Group, Hammersmith Hospital Campus, Imperial College, DuCane Road, London, UK
    Cell Metab 14:707-14. 2011
  2. ncbi request reprint Bypassing the glucose/fatty acid cycle: AMP-activated protein kinase
    D Carling
    MRC Clinical Sciences Centre, Cellular Stress Group, Imperial College, Hammersmith Hospital Campus, DuCane Road, London W12 ONN, U K
    Biochem Soc Trans 31:1157-60. 2003
  3. doi request reprint The regulation of AMP-activated protein kinase by upstream kinases
    D Carling
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, London, UK
    Int J Obes (Lond) 32:S55-9. 2008
  4. ncbi request reprint AMPK
    David Carling
    MRC Clinical Sciences Centre, Cellular Stress Group, Imperial College, Hammersmith Hospital, DuCane Road, London W12 0NN, UK
    Curr Biol 14:R220. 2004
  5. ncbi request reprint The AMP-activated protein kinase cascade--a unifying system for energy control
    David Carling
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London, W12 0NN, UK
    Trends Biochem Sci 29:18-24. 2004
  6. pmc Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding
    P C Cheung
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, U K
    Biochem J 346:659-69. 2000
  7. pmc The regulation of AMP-activated protein kinase by phosphorylation
    S C Stein
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, DuCane Road, London W12 0NN, U K
    Biochem J 345:437-43. 2000
  8. ncbi request reprint The SNF1 kinase complex from Saccharomyces cerevisiae phosphorylates the transcriptional repressor protein Mig1p in vitro at four sites within or near regulatory domain 1
    F C Smith
    MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, UK
    FEBS Lett 453:219-23. 1999
  9. ncbi request reprint Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle
    C Thornton
    Medical Research Council Clinical Sciences Centre, Cellular Stress Group, Imperial College School of Medicine, Hammersmith Hospital, DuCane Road, London W12 0NN, United Kingdom
    J Biol Chem 273:12443-50. 1998
  10. ncbi request reprint The alpha1 and alpha2 isoforms of the AMP-activated protein kinase have similar activities in rat liver but exhibit differences in substrate specificity in vitro
    A Woods
    MRC Molecular Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK
    FEBS Lett 397:347-51. 1996

Collaborators

Detail Information

Publications61

  1. pmc ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein kinase
    Faith V Mayer
    MRC Clinical Sciences Centre, Cellular Stress Group, Hammersmith Hospital Campus, Imperial College, DuCane Road, London, UK
    Cell Metab 14:707-14. 2011
    ..Mutagenesis experiments suggest that the general mechanism by which ADP protects against dephosphorylation is strongly conserved between SNF1 and AMPK...
  2. ncbi request reprint Bypassing the glucose/fatty acid cycle: AMP-activated protein kinase
    D Carling
    MRC Clinical Sciences Centre, Cellular Stress Group, Imperial College, Hammersmith Hospital Campus, DuCane Road, London W12 ONN, U K
    Biochem Soc Trans 31:1157-60. 2003
    ....
  3. doi request reprint The regulation of AMP-activated protein kinase by upstream kinases
    D Carling
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, London, UK
    Int J Obes (Lond) 32:S55-9. 2008
    ..This review focuses on the role of LKB1 and CaMKKbeta in the regulation of AMPK...
  4. ncbi request reprint AMPK
    David Carling
    MRC Clinical Sciences Centre, Cellular Stress Group, Imperial College, Hammersmith Hospital, DuCane Road, London W12 0NN, UK
    Curr Biol 14:R220. 2004
  5. ncbi request reprint The AMP-activated protein kinase cascade--a unifying system for energy control
    David Carling
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London, W12 0NN, UK
    Trends Biochem Sci 29:18-24. 2004
    ..AMPK could provide a link in human diseases of which the underlying cause is due to defects in energy metabolism...
  6. pmc Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding
    P C Cheung
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, U K
    Biochem J 346:659-69. 2000
    ..Labelling studies, using the reactive AMP analogue 8-azido-[(32)P]AMP, indicate that the gamma subunit may participate directly in the binding of AMP within the complex...
  7. pmc The regulation of AMP-activated protein kinase by phosphorylation
    S C Stein
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, DuCane Road, London W12 0NN, U K
    Biochem J 345:437-43. 2000
    ..Furthermore, we provide evidence that phosphorylation of Thr(172) may be involved in the sensitivity of the AMPK complex to AMP...
  8. ncbi request reprint The SNF1 kinase complex from Saccharomyces cerevisiae phosphorylates the transcriptional repressor protein Mig1p in vitro at four sites within or near regulatory domain 1
    F C Smith
    MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, UK
    FEBS Lett 453:219-23. 1999
    ..The latter three are exact matches to the recognition motif we previously defined for SNF1 and lie within regions shown to be required for SNF1-dependent derepression and nuclear-to-cytoplasmic translocation...
  9. ncbi request reprint Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle
    C Thornton
    Medical Research Council Clinical Sciences Centre, Cellular Stress Group, Imperial College School of Medicine, Hammersmith Hospital, DuCane Road, London W12 0NN, United Kingdom
    J Biol Chem 273:12443-50. 1998
    ..These results suggest that the beta isoforms have tissue-specific roles, which may involve altered responses to upstream signaling and/or downstream targeting of the AMPK complex...
  10. ncbi request reprint The alpha1 and alpha2 isoforms of the AMP-activated protein kinase have similar activities in rat liver but exhibit differences in substrate specificity in vitro
    A Woods
    MRC Molecular Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK
    FEBS Lett 397:347-51. 1996
    ..Using variant peptides, however, we show that alpha1 and alpha2 exhibit slightly different substrate preferences, which suggest that the two isoforms could play different physiological roles within the cell...
  11. doi request reprint The regulation and function of mammalian AMPK-related kinases
    N J Bright
    Cellular Stress Group, MRC Clinical Sciences Centre, London, UK
    Acta Physiol (Oxf) 196:15-26. 2009
    ..This review outlines the general similarities in structure and function of the AMPK-related kinases before examining the specific characteristics of each, including a brief discussion of the SNRK family...
  12. ncbi request reprint AMP-activated protein kinase and the metabolic syndrome
    L G D Fryer
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, DuCane Road, London W12 0NN, UK
    Biochem Soc Trans 33:362-6. 2005
    ..Indeed, a number of physiological and pharmacological factors that are beneficial in these disorders have been shown to act, at least in part, through the activation of AMPK...
  13. ncbi request reprint Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro
    A Woods
    Medical Research Council Clinical Sciences Centre, Department of Molecular Medicine, Royal Postgraduate Medical School, DuCane Road, London W12 0NN, United Kingdom
    J Biol Chem 271:10282-90. 1996
    ..These results suggest that AMPKbeta mediates the association of the heterotrimeric AMPK complex in vitro, and will facilitate future studies aimed at investigating the regulation of AMPK in vivo...
  14. ncbi request reprint Ca2+/calmodulin-dependent protein kinase kinase-beta acts upstream of AMP-activated protein kinase in mammalian cells
    Angela Woods
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London W12 0NN, United Kingdom
    Cell Metab 2:21-33. 2005
    ..These results demonstrate that AMPK is regulated by at least two upstream kinases and suggest that AMPK may play a role in Ca(2+)-mediated signal transduction pathways...
  15. ncbi request reprint AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism
    Barbara B Kahn
    Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Cell Metab 1:15-25. 2005
    ..Through signaling, metabolic, and gene expression effects, AMPK enhances insulin sensitivity and fosters a metabolic milieu that may reduce the risk for obesity and type 2 diabetes...
  16. ncbi request reprint Insulin antagonizes ischemia-induced Thr172 phosphorylation of AMP-activated protein kinase alpha-subunits in heart via hierarchical phosphorylation of Ser485/491
    Sandrine Horman
    Hormone and Metabolic Research Unit, Christian de Duve Institute of Cellular Pathology and Universite catholique de Louvain, Avenue Hippocrate 75, B 1200 Brussels, Belgium
    J Biol Chem 281:5335-40. 2006
    ....
  17. ncbi request reprint CNTF reverses obesity-induced insulin resistance by activating skeletal muscle AMPK
    Matthew J Watt
    Cellular and Molecular Metabolism Laboratory, School of Medical Sciences, Royal Melbourne Institute of Technology, PO Box 71, Bundoora, 3083, Australia
    Nat Med 12:541-8. 2006
    ....
  18. ncbi request reprint Activation of AMPK alpha- and gamma-isoform complexes in the intact ischemic rat heart
    Ji Li
    Section of Cardiovascular Medicine, 3 FMP, Yale Univ School of Medicine, 333 Cedar St, New Haven, CT 06520 8017, USA
    Am J Physiol Heart Circ Physiol 291:H1927-34. 2006
    ..Despite the pathophysiological importance of gamma(2)-isoform mutations, gamma(1)-isoform complexes account for most of the AMPK activity in the ischemic heart...
  19. ncbi request reprint Exercise in rats does not alter hypothalamic AMP-activated protein kinase activity
    Ulrika Andersson
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Campus, DuCane Road, London W12 0NN, UK
    Biochem Biophys Res Commun 329:719-25. 2005
    ..Thus, changes in energy metabolism during or after exercise are likely not coordinated by changes in hypothalamic AMPK activity...
  20. pmc Thrombin activates AMP-activated protein kinase in endothelial cells via a pathway involving Ca2+/calmodulin-dependent protein kinase kinase beta
    Nadine Stahmann
    Institute of Molecular Cell Biology, Friedrich Schiller University of Jena, Nonnenplan 2, 07743 Jena, Germany
    Mol Cell Biol 26:5933-45. 2006
    ..We also demonstrate that endothelial cells possess two pathways to activate AMPK, one Ca2+/CaMKKbeta dependent and one AMP/LKB1 dependent...
  21. pmc Investigating the mechanism for AMP activation of the AMP-activated protein kinase cascade
    Matthew J Sanders
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
    Biochem J 403:139-48. 2007
    ..This model accounts for activation of AMPK by two distinct signals: a Ca2+-dependent pathway, mediated by CaMKKbeta and an AMP-dependent pathway, mediated by LKB1...
  22. ncbi request reprint Adiponectin-induced endothelial nitric oxide synthase activation and nitric oxide production are mediated by APPL1 in endothelial cells
    Kenneth K Y Cheng
    Department of Medicine, University of Hong Kong, Hong Kong, China
    Diabetes 56:1387-94. 2007
    ..Taken together, these results suggest that APPL1 acts as a common downstream effector of AdipoR1 and -R2, mediating adiponectin-evoked endothelial NO production and endothelium-dependent vasodilation...
  23. doi request reprint Hypothalamic fatty acid metabolism mediates the orexigenic action of ghrelin
    Miguel Lopez
    Department of Physiology, School of Medicine, University of Santiago de Compostela, S Francisco s n, 15782 Santiago de Compostela A Coruña, Spain
    Cell Metab 7:389-99. 2008
    ..Overall, our results indicate that modulation of hypothalamic fatty acid metabolism specifically in the VMH in response to ghrelin is a physiological mechanism that controls feeding...
  24. doi request reprint Muscarinic receptor activation of AMP-activated protein kinase inhibits orexigenic neuropeptide mRNA expression
    Claire Thornton
    Medical Research Council MRC Cellular Stress Group, MRC Clinical Sciences Centre, Du Cane Road, London W12 0NN, United Kingdom
    J Biol Chem 283:17116-22. 2008
    ..These data support a hypothesis that modulating basal AMPK activity in the hypothalamus is essential for maintaining tight regulation of pathways contributing to food intake...
  25. pmc Investigating the regulation of brain-specific kinases 1 and 2 by phosphorylation
    Nicola J Bright
    Medical Research Council Cellular Stress Group, MRC Clinical Sciences Centre, Du Cane Road, London, United Kingdom
    J Biol Chem 283:14946-54. 2008
    ..Furthermore, stimuli that activate AMPK had no effect on BRSK1/2. Finally, we provide evidence suggesting that protein phosphatase 2C is a likely candidate for catalyzing the dephosphorylation and inactivation of BRSK1/2...
  26. ncbi request reprint The role of the AMP-activated protein kinase in the regulation of energy homeostasis
    David Carling
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
    Novartis Found Symp 286:72-81; discussion 81-5, 162-3, 196-203. 2007
    ..AMPK, therefore, provides a mechanism for monitoring changes in energy metabolism within individual cells and at the level of the whole body...
  27. ncbi request reprint Biochemical and genetic evaluation of the role of AMP-activated protein kinase in polysaccharide storage myopathy in Quarter Horses
    Patricia K Dranchak
    Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA
    Am J Vet Res 68:1079-84. 2007
    ..To evaluate whether biochemical or genetic alterations in AMP-activated protein kinase (AMPK) play a role in the development of polysaccharide storage myopathy (PSSM) in Quarter Horses...
  28. ncbi request reprint Structural basis for AMP binding to mammalian AMP-activated protein kinase
    Bing Xiao
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 449:496-500. 2007
    ..The structure also suggests a mechanism for propagating AMP/ATP signalling whereby a phosphorylated residue from the alpha and/or beta subunits binds to the gamma subunit in the presence of AMP but not when ATP is bound...
  29. ncbi request reprint Defining the mechanism of activation of AMP-activated protein kinase by the small molecule A-769662, a member of the thienopyridone family
    Matthew J Sanders
    Cellular Stress Group, Medical Research Council Clinical Sciences Centre, Hammersmith Hospital, Imperial College, DuCane Road, London, United Kingdom
    J Biol Chem 282:32539-48. 2007
    ..Importantly, this model may provide clues for understanding the mechanism by which AMP leads to activation of AMPK, which in turn may help in the identification of other AMPK activators...
  30. pmc AMPK is essential for energy homeostasis regulation and glucose sensing by POMC and AgRP neurons
    Marc Claret
    Centre for Diabetes and Endocrinology, Rayne Institute, University College London, London, United Kingdom
    J Clin Invest 117:2325-36. 2007
    ....
  31. ncbi request reprint S6 kinase deletion suppresses muscle growth adaptations to nutrient availability by activating AMP kinase
    Victor Aguilar
    INSERM, U845, Paris F 75015, France Université Paris Descartes, UMRS 845, Paris F 75015, France
    Cell Metab 5:476-87. 2007
    ..These data indicate that S6K1 controls the energy state of the cell and the AMPK-dependent metabolic program, providing a mechanism for cell mass accumulation under high-calorie diet...
  32. ncbi request reprint AMP-activated protein kinase: balancing the scales
    David Carling
    MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, DuCane Road, London, W12 0NN, UK
    Biochimie 87:87-91. 2005
    ..Taken together these findings indicate that AMPK has a pivotal role in regulating pathways that control both energy expenditure and energy intake...
  33. pmc Transgenic mouse model of ventricular preexcitation and atrioventricular reentrant tachycardia induced by an AMP-activated protein kinase loss-of-function mutation responsible for Wolff-Parkinson-White syndrome
    Jasvinder S Sidhu
    Baylor College of Medicine, Houston, Tex, USA
    Circulation 111:21-9. 2005
    ..The human phenotype consists of ventricular preexcitation, conduction abnormalities, and cardiac hypertrophy...
  34. pmc The AMP-activated protein kinase alpha2 catalytic subunit controls whole-body insulin sensitivity
    Benoit Viollet
    Department of Genetic, Development, and Molecular Pathology, Institut National de la Sante et de la Recherche Medicale INSERM, Centre National de la Recherche Scientifique CNRS, Rene Descartes University, Paris, France
    J Clin Invest 111:91-8. 2003
    ..Therefore, extramuscular AMPKalpha2 catalytic subunit is important for whole-body insulin action in vivo, probably through modulation of sympathetic nervous activity...
  35. ncbi request reprint Regulation of glycogen synthase by glucose and glycogen: a possible role for AMP-activated protein kinase
    Reza Halse
    School of Biochemistry and Genetics, Medical School, University of Newcastle, Newcastle upon Tyne, U K
    Diabetes 52:9-15. 2003
    ..These data support a role for AMPK in both stimulating glucose uptake and inhibiting GS in intact cells, thus promoting glucose flux through glycolysis...
  36. ncbi request reprint Protein kinase inhibitors block the stimulation of the AMP-activated protein kinase by 5-amino-4-imidazolecarboxamide riboside
    Lee G D Fryer
    Cellular Stress Group, MRC Clinical Sciences Centre, Hammersmith Hospital, DuCane Road, London, UK
    FEBS Lett 531:189-92. 2002
    ..Our results demonstrate that careful interpretation is required when using AICA riboside in conjunction with protein kinase inhibitors to investigate the physiological role of AMPK...
  37. ncbi request reprint Functional analysis of mutations in the gamma 2 subunit of AMP-activated protein kinase associated with cardiac hypertrophy and Wolff-Parkinson-White syndrome
    Tyrone Daniel
    Cellular Stress Group, MRC Clinical Sciences Centre, Hammersmith Hospital, DuCane Road, London W12 0NN, United Kingdom
    J Biol Chem 277:51017-24. 2002
    ..These results indicate that mutations in gamma(2) have different effects on AMPK function, suggesting that they may lead to abnormal development of the heart through distinct mechanisms...
  38. ncbi request reprint Isoform-specific regulation of 5' AMP-activated protein kinase in skeletal muscle from obese Zucker (fa/fa) rats in response to contraction
    Brian R Barnes
    Department of Clinical Physiology and Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden
    Diabetes 51:2703-8. 2002
    ..Activation of AMPKalpha2 in response to muscle contraction/ exercise is associated with a parallel and normal increase in glucose transport in insulin-resistant skeletal muscle...
  39. ncbi request reprint Activation of GLUT1 by metabolic and osmotic stress: potential involvement of AMP-activated protein kinase (AMPK)
    Kay Barnes
    School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    J Cell Sci 115:2433-42. 2002
    ..These enzymes, which have been implicated in stress-regulated pathways in other cell types, are therefore unlikely to play a role in transport regulation by stress in Clone 9 cells...
  40. ncbi request reprint The Anti-diabetic drugs rosiglitazone and metformin stimulate AMP-activated protein kinase through distinct signaling pathways
    Lee G D Fryer
    Cellular Stress Group, Medical Research Council Clinical Sciences Centre, Hammersmith Hospital, DuCane Road, London W12 0NN, United Kingdom
    J Biol Chem 277:25226-32. 2002
    ..Our results demonstrate that AMPK can be activated by at least two distinct signaling mechanisms and suggest that it may play a wider role in the cellular stress response than was previously understood...
  41. pmc AMP-activated kinase regulates cytoplasmic HuR
    Wengong Wang
    Laboratory of Cellular and Molecular Biology, National Institute on Aging Internal Research Program, National Institutes of Health, Baltimore, Mayland 21224 6825, USA
    Mol Cell Biol 22:3425-36. 2002
    ..We therefore propose a novel function for AMPK as a regulator of cytoplasmic HuR levels, which in turn influences the mRNA-stabilizing function of HuR and the expression of HuR target transcripts...
  42. pmc Expression and regulation of the AMP-activated protein kinase-SNF1 (sucrose non-fermenting 1) kinase complexes in yeast and mammalian cells: studies using chimaeric catalytic subunits
    Tyrone Daniel
    Cellular Stress Group, MRC Clinical Sciences Centre, Hammersmith Hospital, Du Cane Road, London W12 0NN, U K
    Biochem J 365:629-38. 2002
    ..In yeast, the glucose signalling pathway is able to activate AMPK, suggesting that the mammalian and yeast kinase pathways are conserved...
  43. pmc Characterization of the role of the AMP-activated protein kinase in the stimulation of glucose transport in skeletal muscle cells
    Lee G D Fryer
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, DuCane Road London, W12 0NN, UK
    Biochem J 363:167-74. 2002
    ..Our results add further support to the hypothesis that long-term activation of AMPK is involved in the adaptive response of muscle to exercise training...
  44. ncbi request reprint Leptin stimulates fatty-acid oxidation by activating AMP-activated protein kinase
    Yasuhiko Minokoshi
    Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Nature 415:339-43. 2002
    ..Blocking AMPK activation inhibits the phosphorylation of ACC stimulated by leptin. Our data identify AMPK as a principal mediator of the effects of leptin on fatty-acid metabolism in muscle...
  45. ncbi request reprint Increased AMP:ATP ratio and AMP-activated protein kinase activity during cellular senescence linked to reduced HuR function
    Wengong Wang
    Laboratory of Cellular and Molecular Biology, NIA Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 278:27016-23. 2003
    ..Together, our results indicate that AMPK activation can cause premature fibroblast senescence through mechanisms that likely involve reduced HuR function...
  46. ncbi request reprint Hyperglycemia-induced apoptosis in human umbilical vein endothelial cells: inhibition by the AMP-activated protein kinase activation
    Yasuo Ido
    Boston Medical Center, EBRC 820, Diabetes and Metabolism Unit, Section of Endocrinology and Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Diabetes 51:159-67. 2002
    ..They also suggest that AMPK could play an important role in protecting the endothelial cell against the adverse effects of sustained hyperglycemia...
  47. ncbi request reprint AMP-activated protein kinase plays a role in the control of food intake
    Ulrika Andersson
    Medical Research Council Clinical Sciences Centre, Cellular Stress Group and Endocrine Unit, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 ONN, United Kingdom
    J Biol Chem 279:12005-8. 2004
    ..These findings demonstrate that AMPK plays a role in the regulation of feeding and identify AMPK as a novel target for anti-obesity drugs...
  48. ncbi request reprint Thr2446 is a novel mammalian target of rapamycin (mTOR) phosphorylation site regulated by nutrient status
    Susan W Y Cheng
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    J Biol Chem 279:15719-22. 2004
    ..These data suggest differential phosphorylation Thr2446 and Ser2448 could act as a switch mechanism to integrate signals from nutrient status and growth factors to control the regulation of protein translation...
  49. ncbi request reprint LKB1 is the upstream kinase in the AMP-activated protein kinase cascade
    Angela Woods
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, Du Cane Road, London W12 0NN, United Kingdom
    Curr Biol 13:2004-8. 2003
    ..These results identify a link between two protein kinases, previously thought to lie in unrelated, distinct pathways, that are associated with human diseases...
  50. ncbi request reprint Mammalian AMP-activated protein kinase: functional, heterotrimeric complexes by co-expression of subunits in Escherichia coli
    Dietbert Neumann
    Institute of Cell Biology, Swiss Federal Institute of Technology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Protein Expr Purif 30:230-7. 2003
    ....
  51. pmc Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases
    Seung Pyo Hong
    Department of Genetics and Development, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 100:8839-43. 2003
    ..Thus, the identification of the yeast upstream kinases should facilitate identification of the corresponding, physiologically important mammalian upstream kinases...
  52. ncbi request reprint Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids
    Hilary Clark
    Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK
    Eur J Biochem 271:2215-24. 2004
    ..The concept is discussed that activation of AMPK by an unknown fatty acid-driven signalling process provides a mechanism for a 'feed-forward' activation of fatty acid oxidation...
  53. ncbi request reprint Characterization of the role of gamma2 R531G mutation in AMP-activated protein kinase in cardiac hypertrophy and Wolff-Parkinson-White syndrome
    Joanna K Davies
    Cellular Stress Group, Medical Research Council Clinical Sciences Centre, Hammersmith Campus, Imperial College London, London W12 ONN, UK
    Am J Physiol Heart Circ Physiol 290:H1942-51. 2006
    ..The subsequent decrease in AMPK activity provides a mechanism that may explain the development of cardiac hypertrophy in this model...
  54. ncbi request reprint Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis
    Angela Woods
    Hormone and Metabolic Research Unit, Christian de Duve Institute of Cellular Pathology and Université de Louvain, Avenue Hippocrate 75, B 1200 Brussels, Belgium
    J Biol Chem 278:28434-42. 2003
    ..The newly identified phosphorylation sites could play a subtle role in the regulation of AMPK, e.g. in subcellular localization or substrate recognition...
  55. ncbi request reprint Neuregulin signaling on glucose transport in muscle cells
    Carles Canto
    Departament de Bioquímica i Biologia Molecular and Parc Científic de Barcelona, Universitat de Barcelona, Avda Diagonal 645, E 08028 Barcelona, Spain
    J Biol Chem 279:12260-8. 2004
    ..The PI3K, PDK1, and PKCzeta pathway can be considered as an alternative mechanism, independent of insulin, to induce glucose uptake...
  56. ncbi request reprint Cellular energy sensor balances the scales
    Caroline J Small
    Nat Med 10:681-2. 2004
  57. ncbi request reprint LKB1: a sweet side to Peutz-Jeghers syndrome?
    David Carling
    Cellular Stress Group, MRC Clinical Sciences Centre, Imperial College, Hammersmith Hospital, DuCane Road, London W12 0NN, UK
    Trends Mol Med 12:144-7. 2006
    ..These findings should help solve the mystery surrounding the function of metformin, which has lasted for >30 years...
  58. ncbi request reprint Low utilization of circulating glucose after food withdrawal in Snell dwarf mice
    Natasha L Brooks
    Department of Nutrition, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:35069-77. 2007
    ....
  59. ncbi request reprint Tumor necrosis factor alpha-induced skeletal muscle insulin resistance involves suppression of AMP-kinase signaling
    Gregory R Steinberg
    St Vincent s Institute and Department of Medicine, University of Melbourne, Fitzroy, Vic, 3065, Australia
    Cell Metab 4:465-74. 2006
    ..Our data demonstrate that AMPK is an important TNFalpha signaling target and is a contributing factor to the suppression of fatty-acid oxidation and the development of lipid-induced insulin resistance in obesity...
  60. pmc A conserved sequence immediately N-terminal to the Bateman domains in AMP-activated protein kinase gamma subunits is required for the interaction with the beta subunits
    Rosa Viana
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, 46010 Valencia, Spain
    J Biol Chem 282:16117-25. 2007
    ..In addition, sequential deletions from the C termini of the gamma subunits indicate that deletion of any of the CBS (cystathionine beta-synthase) motifs prevents the formation of a functional complex with the alpha and beta subunits...
  61. pmc Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase
    Nobuyuki Kuramoto
    Department of Neuroscience, University of Pennsylvania, Philadelphia, PA 19104, USA
    Neuron 53:233-47. 2007
    ..Together our results provide evidence of a neuroprotective mechanism, which, under conditions of metabolic stress or after ischemia, increases GABA(B) receptor function to reduce excitotoxicity and thereby promotes neuronal survival...